The purpose of this study is to identify the differences in the perception of the therapeutic effects of interior environmental factors in relation to the stress levels of young adults in their 20s, who are the generation experiencing the highest stress levels. This research seeks to provide evidence-based results for a user-centered therapeutic environmental plan. Five studies from abroad examining therapeutic environmental factors were analyzed and subsequently reorganized to be utilized as an investigative tool. The Psychosocial Wellbeing Index Scale-Short Form (PWI-SF) was employed to measure the stress levels of young adults in their 20s. Further, 411 adults in this age group completed an online questionnaire to assess their general perception of stressful environments and that of the therapeutic effects of interior environmental factors. The results are as follows: First, the group with higher stress levels had a higher level of stress regarding the environment and a lower level of satisfaction with space. Second, the higher the stress, the more the participants tended to perceive the therapeutic effects of interior environmental factors negatively. Third, regardless of their stress levels, the respondents noted that the protection of privacy and provision of personal territory helped alleviate stress. Fourth, different perceptions of therapeutic effects were revealed toward positive distraction and interaction-supporting space factors in relation to stress levels. Fifth, there was a high level of perception that factors related to humidity control, sanitation, and finishing materials did not help with stress relief.