Estuary Environment Research Articles

Influence of Aquaculture Environments on the Muscle Quality of Golden Pompano (Trachinotus ovatus) in the Beibu Gulf: A Multifaceted Analysis of Nutritional, Textural, and Flavor Profiles

Golden Pompano (Trachinotus ovatus) is a primary source of high-quality protein, yet its muscle quality is significantly influenced by varying aquaculture environments, impacting both nutritional value and marketability. This study aimed to assess the muscle quality of T. ovatus across estuary, nearshore, and offshore aquaculture environments in the Beibu Gulf. We employed standardized protocols to evaluate key quality parameters including basic nutritional components, amino acids, fatty acids, volatile substances, flavor nucleotides, and thermal stability through heat analysis. Data analysis was conducted using statistical comparisons across different environments to identify significant differences. Our results indicated that offshore aquaculture environments yielded T. ovatus with superior nutritional profiles, exhibiting higher moisture, ash, crude protein, and crude fat contents. Additionally, offshore-cultivated fish demonstrated better textural qualities and higher levels of essential amino acids, unsaturated fatty acids, and umami-flavor nucleotides. These findings highlight that offshore aquaculture environments optimize the muscle quality of T. ovatus, suggesting implications for environmental management and enhancing fish meat quality in aquaculture practices.

Pollen evidence of enhanced Yellow River provenance into the South Yellow Sea after the Middle Pleistocene Transition

Quaternary sediment of the South Yellow Sea (SYS) is usually assumed to record climatic changes, tectonic activities and major river evolutions. However, as an important terrigenous indicator, a long sequence of pollen proxy in the SYS lacks for studies of river provenance and climate changes. In this study, we focused on a 150-m pollen record in the SYS that covered most of the Quaternary period over the past 1.8 Ma. The most significant changes of pollen assemblage were caused by river provenance, that high pollen percentages of broadleaved trees such as Castanea/Castanopsis reflect a dominant provenance of the Yangtze River since ca. 1.8 Ma, with a decline trend during the Mid-Pleistocene transition (MPT) mainly between 1.1 and 0.8 Ma. The sharp increase in coniferous pollen, particularly Picea, originated from the Yellow River drainage areas, suggesting a major alteration in terrigenous pollen source from the Yangtze River to the Yellow River at ∼0.8 Ma, which agrees well with the timing of the final integration of the modern Yellow River. The high pollen quantities of Artemisia and Ephedra also shows the enhanced Yellow River provenance where most landscape was covered by semi-arid open vegetation (e.g. steppe). The alternate appearances of marine foraminifera and dinoflagellate cysts, and freshwater wetland plants (Cyperaceae, Poaceae) through the interglacial intervals from MIS 19/21 demonstrate different estuary sedimentary environments and diverse salinity, associated with the sea-level fluctuations. The pollen concentration suggests an increase in terrigenous materials towards the interglacial MIS 11 and the preliminary delta formation of the Yellow River. We propose that the terrigenous input from the Yellow River accelerated after the MPT, and combined effects of tectonic movement drove the evolution of the river delta plains in eastern China. Our data also reveal some secondary periodic changes in evergreen broadleaved forest during the past 1.8 Ma and cold-tolerant coniferous forest since particularly the MPT, which are generally linked with the 400-ka long eccentricity cycle and the variability of monsoon climate.

Benign water quality and phytoplankton status during the operation of Guishan offshore wind farm in the Pearl River Estuary, China

Wind power is a clean and renewable source of energy that plays a crucial role in the global effort to combat climate change. Yet, the effects of offshore wind farms on the marine environment remain poorly understood and there is a lack of field data analysis in this area. This study aims to address these gaps by conducting a year-long examination of water quality and phytoplankton communities around the Guishan offshore wind farm (GS-OWF) in the Lingdingyang Bay, Pearl River Estuary, China. The analysis of water quality indicators reveals repelling effect on algae dispersion but no significant contamination resulting from the running of the wind farm over the course of one year. These indicators encompassed various parameters, such as water depth (4.1 − 17.4 m), salinity (16.9 – 28.4 ‰), pH (7.41 – 8.35), temperature (20.5 − 31.2 °C), transparency (⁓ 1.5 m), dissolved oxygen (⁓ 7.5 mg L−1), chemical oxygen demand (⁓ 0.81 mg L−1), chlorophyll-a (14.7 − 0.139 μg L−1), petroleum (0 – 0.0527 mg L−1), and various heavy metals. The cell composition (4 phyla, 158 species, dominated by Bacillariophyta) and density (3 – 1809 cells m−3) demonstrate a healthy phytoplankton community. These results indicate a benign estuary environment after the construction of GS-OWF. This study provides valuable insights into the environmental effects of offshore wind farms, principles for site selection, and the integration of aquaculture convergence with offshore wind farms.

Spatial and temporal distribution of juvenile Gulf Sturgeon in the Pearl River, Louisiana, and adjacent estuaries of the Gulf of Mexico

AbstractObjectiveAcoustic telemetry was used to examine the spatial‐temporal distribution and anadromous migration pattern of juvenile Gulf Sturgeon Acipenser desotoi within the Pearl River and associated coastal estuaries of Louisiana and Mississippi.MethodsAcoustic transmitters were surgically implanted in 52 juvenile Gulf Sturgeon (<890 mm fork length) from the Pearl River between 2016 and 2019. Sturgeon were monitored on 247 acoustic receivers in the lower Pearl River and adjacent estuaries across numerous partnering arrays.ResultJuveniles were detected on receivers from Lake Pontchartrain, Louisiana, east to Bay St. Louis, and Biloxi Bay, Mississippi. The 3‐year study found that juvenile Gulf Sturgeon migration in the Pearl River occurred on cycles similar to those reported from other populations. Juveniles were rarely observed in the estuary and nearshore marine environments from May through mid‐October of each year, during which time most sturgeon inhabited the Pearl River. Conversely, most of the juveniles inhabited coastal estuaries from December through February of each year and were rarely observed in freshwater environments during this period. Migration from the river into the estuary occurred during late‐October through November, and migration from the estuary into the river occurred in March and April. Distribution patterns varied spatially within the river and estuary ecosystems during each phase of migration. Tagged juveniles occupied the lowest 40 km of the Pearl River while in freshwater, and they occupied the northeastern shoreline of Lake Pontchartrain and Bay St. Louis while in the estuary. Interestingly, juveniles seemed to target specific areas in the estuary during winter that were farther away from the mouth of natal rivers than has been previously reported.ConclusionThis study provides a new assessment of juvenile Gulf Sturgeon migration and seasonal distribution for the Pearl River and provides important information for designing and assessing the potential benefits and challenges of habitat restoration for Gulf Sturgeon.

Bioaccumulation and trophic transfer of per- and polyfluoroalkyl substances in a subtropical mangrove estuary food web

Mangrove estuaries are an important land-sea transitional ecosystem that is currently under various pollution pressures, while there is a lack of research on per- and polyfluoroalkyl substances (PFAS) in the organisms of mangrove estuaries. In this study, we investigated the distribution and seasonal variation of PFAS in the tissues of organisms from a mangrove estuary. The PFAS concentrations in fish tissues varied from 0.45 ng/g ww to 17.67 ng/g ww and followed the order of viscera > head > carcass > muscle, with the highest tissue burden found in the fish carcass (39.59 ng). The log BAF values of PFDoDA, PFUnDA, and PFDA in the whole fish exceeded 3.70, indicating significant bioaccumulation. The trophic transfer of PFAS in the mangrove estuary food web showed a dilution effect, which was mainly influenced by the spatial heterogeneity of PFAS distribution in the estuarine environment, and demonstrated that the gradient dilution of PFAS in the estuary habitat environment can disguise the PFAS bio-magnification in estuarine organisms, and the larger the swimming ranges of organisms, the more pronounced the bio-dilution effect. The PFOA-equivalent HRs of category A and B fish were 3.48–5.17 and 2.59–4.01, respectively, indicating that mangrove estuarine residents had a high PFAS exposure risk through the intake of estuarine fish.

Heavy metals assessment for sustainable management in estuaries of Ba Ria - Vung Tau province

Heavy metal concentration in the aquatic environment of estuaries is a significant environmental concern with potential consequences for ecosystems and human health. Heavy metals are naturally occurring elements that can enter aquatic environments through both natural processes and human activities. The interplay between natural processes and human activities, particularly urbanization and industrialization, often exacerbates heavy metal pollution in aquatic ecosystems. Some of the most common heavy metals of concern in estuarine pollution include arsenic (As), cadmium (Cd), mercury (Hg), lead (Pb), zinc (Zn), and iron (Fe). Based on the results of two surveys in rainy and dry seasons, the paper aims to assess the concentrations of heavy metals in the estuarine environment of Ba Ria - Vung Tau Province, Vietnam, to understand the extent of contamination and its potential impacts on the ecosystem and human health. The average concentrations As, Cd, Hg, Pb, Zn, and Fe were 2.01, 0.024, 0.028, 1.63, 4.71, and 492.9 µg/L in water, and 6.70, 0.175, 0.060, 11.78, 41.94, and 3,108.9 mg/kg in sediment, respectively. Heavy metal concentrations in water have an increased trend during the rainy season. In contrast, values in sediment exhibit an opposite pattern, which can be explained by various environmental factors and processes. For water and sediment quality, individual metals, and overall metal contamination levels (the contamination factor - Cf, contamination degree - Cd, and metal pollution index - MPI) may appear to be low, whereas the assessment of the combined effects of multiple metals (Metal quality index - MI > 1) indicates that there is a potential risk to aquatic life in the estuaries. This assessment may underscore the importance of considering not only individual metal concentrations but also their cumulative effects when evaluating water and sediment quality and making decisions regarding protecting aquatic ecosystems. Further investigations and measures may be needed to mitigate potential harm to aquatic organisms and for sustainable management in estuaries.

Dynamic Modeling of Coastal Compound Flooding Hazards Due to Tides, Extratropical Storms, Waves, and Sea-Level Rise: A Case Study in the Salish Sea, Washington (USA)

The Puget Sound Coastal Storm Modeling System (PS-CoSMoS) is a tool designed to dynamically downscale future climate scenarios (i.e., projected changes in wind and pressure fields and temperature) to compute regional water levels, waves, and compound flooding over large geographic areas (100 s of kilometers) at high spatial resolutions (1 m) pertinent to coastal hazard assessments and planning. This research focuses on advancing robust and computationally efficient approaches to resolving the coastal compound flooding components for complex, estuary environments and their application to the Puget Sound region of Washington State (USA) and the greater Salish Sea. The modeling system provides coastal planners with projections of storm hazards and flood exposure for recurring flood events, spanning the annual to 1-percent annual chance of flooding, necessary to manage public safety and the prioritization and cost-efficient protection of critical infrastructure and valued ecosystems. The tool is applied and validated for Whatcom County, Washington, and includes a cross-shore profile model (XBeach) and overland flooding model (SFINCS) and is nested in a regional tide–surge model and wave model. Despite uncertainties in boundary conditions, hindcast simulations performed with the coupled model system accurately identified areas that were flooded during a recent storm in 2018. Flood hazards and risks are expected to increase exponentially as the sea level rises in the study area of 210 km of shoreline. With 1 m of sea-level rise, annual flood extents are projected to increase from 13 to 33 km2 (5 and 13% of low-lying Whatcom County) and flood risk (defined in USD) is projected to increase fifteenfold (from 14 to USD 206 million). PS-CoSMoS, like its prior iteration in California (CoSMoS), provides valuable coastal hazard projections to help communities plan for the impacts of sea-level rise and storms.

Diel Patterns in the Composition and Activity of Planktonic Microbes in a Subtropical Bay

Planktonic microbes play a crucial role in the matter transformation and energy transfer of marine ecosystems. Marine microbial communities are profoundly influenced by various environmental factors, and their variations are typically investigated in a time-dependent manner. However, studies conducted on a monthly or annual basis do not offer sufficient temporal resolution to reveal changes that can occur on an hourly basis. We conducted a high-resolution time-series study using amplicon sequencing on seawater samples taken at 2-h intervals over a 3-d period from the subtropical Daya Bay to investigate changes in the microbial community composition and activity. The results showed that 46.8% of the microbial rRNA sequences exhibited circadian rhythms, including phytoplankton (e.g., Bacillariophyta, Cryptophyta, and Dinophyceae), heterotrophic bacteria (e.g., Actinomarinaceae, Flavobacteriaceae, and SAR11_clade), and zooplankton (e.g., Bestiolina and Phyllopharyngea). The genera of Bacillariophyta (e.g., Chaetoceros and Pseudo-nitzschia ) exhibited higher activity than those of Chlorophyta (e.g., Micromonas ). A similar trend was observed for zooplankton, where Choanozoa (e.g., Copepoda ) showed a strong circadian rhythm in abundance whereas Ciliophora (e.g., Pithites ) were more active. Light intensity and tides were identified as the driving factors behind the periodic succession and activity of the heterotrophic bacteria and phytoplankton with the exception of picocyanobacteria, which were mainly influenced by fluctuations in temperature, nutrients, and salinity. These high-resolution time-dependent observations showed that, despite the dynamic coastal environment, the periodic changes in the microbial community and activity were distinct and phylogenetically conserved. The findings may help in the development of ecosystem models for predicting microbial abundance and activity in rapidly changing coastal and estuary environments.

Application of TRMM for Spatio-Temporal Analysis of Precipitation in the Taiwan Strait and Its Adjacent Regions

Precipitation patterns are highly valued in the fields of weather forecasting, water resource management, and estuary environment research. In this study, daily and monthly precipitation TRMM data from 1998 to 2019 were selected, and EOF analysis was employed to analyze the precipitation patterns of the Taiwan Strait and its neighboring regions. We obtained the following results: (1) The rainy season (May–June) is the main contributor to precipitation in the study area. The EOF first mode reflected the overall consistency of the precipitation spatial distribution. However, within each river basin, the magnitude of precipitation variation is spatially different. The magnitude of precipitation variation is significant in the northwestern part of the Minjiang River basin, the southwestern part of the Jiulong River basin, and the southwestern corner of the Hanjiang River basin. These areas happen to correspond to the mountain areas, revealing that topographic precipitation plays a role in the spatial distribution of precipitation in the three river basins. (2) The spatial distributions of the EOF first mode and of precipitation during El Niño in the Minjiang River basin are consistent. This reveals that ENSO is probably the dominant factor in precipitation in the Minjiang River basin. The significant increase in precipitation during El Niño compared with a normal year in the Minjiang River basin confirms this point. (3) In all three strong El Niño years, 1998, 2010, and 2016, the Minjiang River basin experienced significant heavy precipitation in the fall and winter, whereas the Jiulong River and Hanjiang River basins did not (except in 2016). In other words, the Minjiang River basin is more affected by ENSO, while the Jiulong River and Hanjiang River basins are only limitedly impacted by ENSO.

Microbial iron acquisition is influenced by spatial and temporal conditions in a glacial influenced river and estuary system.

In Arctic regions, glaciers are major sources of iron to rivers and streams; however, estuaries are considered iron sinks due to the coagulation and flocculation processes that occur at higher salinities. It is unknown how iron dynamics in a glacial influenced river and estuary environment affect microbial mechanisms for iron acquisition. Microbial taxonomic and functional sequencing was performed on samples taken throughout the year from the Kenai River and the estuary, Alaska. Despite distinct iron, sodium, and other nutrient concentrations, the river and estuary did not have statistically different microbial communities nor was time of sampling significant. However, ferrous iron transport (Feo) system genes were more abundant in river environments, while siderophore genes were more abundant and diverse in estuary environments. Siderophore transport and iron storage genes were found in all samples, but gene abundance and distribution were potentially influenced by physical drivers such as discharge rates and nutrient distributions. Differences in iron metabolism between river and estuary ecosystems indicate environmental conditions drive microbial mechanisms to sequester iron. This could have implications for iron transport as the Arctic continues to warm.

Environmental and vegetation dynamics through the Oligocene to Early Miocene of North Africa (Egypt)

This study documents palynomorph assemblages from the Oligocene through the Early Miocene, across the significant Warm-house to Cool-house transition, using a subsurface section from the AG-5 well, north Western Desert of Egypt. Although the Paleogene and Neogene strata occupied a large area of the land, little about vegetation and ecosystem processes that shaped the climate and biodiversity during this time window is known. Here, based on a comprehensive palynological analysis of the Oligocene to Early Miocene Dabaa and Moghra formations encountered in the AG-5 well, we show that the studied area was characterized by a humid tropical to subtropical climate during the Oligocene-Miocene time interval. The obtained results provide clear evidence of the poorly known Paleogene-Neogene ecosystem in North Africa. In terms of palynostratigraphical framework, three palynozones covering the late Oligocene-Early Miocene interval are recognized. Moreover, the recovered palynomorphs inferred a deposition of the Dabaa Formation under a normal marine, inner shelf to littoral environment. However, for the basal part of the Moghra Formation, a deposition in an offshore marine environment, outer shelf (high sea level, 100–200 m depth) is assumed, whereas a deposition in shallow coastal lagoons, estuary, or delta environment (low sea level) is presumed for its uppermost part.

A comprehensive study of the estuary sea environment in the Bay of Bengal, near the Mahanadi River confluence

This study focuses on the environmental conditions of the Mahanadi Estuary near Paradeep Harbor and the adjacent sea. Data collected from May 2013 to April 2020 from 32 GPS fixed stations was analyzed to assess the water quality in different zones (estuarine, mixed zone, mixed zone south, and mixed zone north) of study area. Parameters such as pH, SST, TSS, nitrite, phosphate, silicate, TOC, chlorophyll, fecal coliform, and heavy metals were used to estimate the Water Quality Index (WQI) for each zone. The study found a deterioration (> 30%) in the overall water quality of the Mahanadi Estuary from 2013 to 2020, potentially attributed to river inflows, port activities, and industrial outflows in to the coastal ecosystem. Seasonal variations in temperature, salinity, turbidity, nitrite, nitrate, and ammonia were observed. The water quality showed a deteriorating trend in estuarine, mixed zone, mixed zone south, and mixed zone north. Based on the water quality indices, the ecosystem shows moderate levels of stress. The degraded water quality highlights the need for a targeted mitigation plan to reduce external pressures and enhance the overall ecosystem quality.Graphical

Uncovering the distribution patterns and origins of organophosphate esters (OPEs) in the Yellow River Estuary via high-resolution mass spectrometry

Limited information is available regarding the pollution status of organophosphate esters (OPEs) in the environment of the Yellow River estuary. Here, n = 51 sediment samples were collected from the Yellow River estuary in 2021, and further analyzed by using the integrated target, suspect, and feature fragment-dependent nontarget OPE screening strategy developed in our laboratory. Among the 30 target OPEs, 19 were detectable in at least one of the analyzed samples, with total concentrations (Σ19OPEs) ranging from of 41.4 to 1930 ng/g dry weight (dw). On the basis of an in-house suspect compound database, we further tentatively identified 11 suspect OPEs, and they were semi-quantified. Furthermore, four other interesting findings were observed and described as follows: 1) a statistically significant difference existed in the concentrations of OPEs in sediment samples between the lower reaches of the Yellow River (n = 5 samples), and the Yellow River estuary (n = 46 samples) (unpaired t-test, p < 0.001); 2) tris(2,4-di-tert-butylphenyl)phosphate (TDTBPP) exhibited the greatest concentrations (ranging from 30.7 to 1920 ng/g dw) among all OPEs detected in the sediment samples; 3) samples from the north of the Yellow River estuary had higher OPE concentrations than those from the south; and 4) a suspect screening strategy allowed us to identify a novel OPE structure (tert-butyl)phenyl (ethyne-oxidane) bis(2,4-di-tert-butylphenyl) phosphate (TPBDTP) that exhibited a highly positive correlation relationship with TDTBPP (r = 0.749; p < 0.001). Overall, this study provided evidence that OPEs (especially TDTBPP) were ubiquitous in the sediment environment of the Yellow River estuary; thus, we emphasize that continuous monitoring of OPE pollution should be conducted in this region.

Growth retardation and suppression of ubiquitin-dependent catabolic processes in the brackish water clam Corbicula japonica in response to salinity changes and bioaccumulation of toxic heavy metals

The brackish water clam (Corbicula japonica) is constantly exposed to stressful salinity gradients and high levels of heavy metals in the freshwater-saltwater interface of estuary environments, which are introduced from upstream regions and land. To identify the key molecular pathways involved in the response to salinity changes and heavy metal bioaccumulation, we obtained the transcriptomes of C. japonica inhabiting different salinities and heavy metal distributions in Gwangyang Bay (Korea) using RNA sequencing. Among a total of 404,486 assembled unigenes, 5534 differentially expressed genes were identified in C. japonica inhabiting different conditions, 1549 of which were significantly upregulated and 1355 were significantly downregulated. Correlation analyses revealed distinct gene expression patterns between the low and high conditions of salinity and heavy metal bioaccumulation. Functional annotation revealed significant downregulation of genes involved in “ubiquitin-dependent protein catabolic process,” “tricarboxylic acid cycle,” and “intracellular protein transport” in C. japonica from the high condition compared to the low condition. Transcription and translation pathways were significantly enriched in the high condition. Additionally, upon comparison of the low and high conditions by qRT-PCR and proteasome enzyme activity analyses, our findings demonstrated that environmental stress could suppress the ubiquitin-proteasome complex (UPC). Additionally, transcriptomic changes under high salinity stress conditions may be related to an increase in cellular protection by defense enzymes, which leads to more energy being required and a disruption of energy homeostasis. Ultimately, this could cause growth retardation in the clam C. japonica. In summary, this study provides the first evidence of UPC suppression induced by a combination of high salinity and heavy metal bioaccumulation stress in C. japonica, which could compromise the survival and growth of estuarine bivalves.

Influence of the spatial variation of upstream velocity on a vertical-axis tidal turbine performance

Assessment of the upstream flow available to the tidal energy converters (TEC) is key to evaluate its performance. Simultaneously, TEC technology has been innovating on its concepts and designs to expand the potential sites to harvest energy generated by tidal currents and rivers. The Gkinetic CEFA 12 is an easy-to-deploy device suitable to operate on estuary environments. The design consists of two 1.2 m vertical axis turbines attached to the sides of a buoyant platform, which uses a bluff body to accelerate the incoming flow to the rotors. The device was deployed on a single point mooring enabling passive flow alignment. As part of the Vertical axis tidal turbines in Strangford lough project (VATTS), the upstream flow has been measured using acoustic doppler profilers (ADP) mounted on the TEC during its operation in Strangford lough. Recommendations of IEC-200 were followed when mounting the ADPs relative to Gkinetic. However, the continuous repositioning of the TEC according to the prevailing tidal regime affects the ADPs heading. The implications of the spatial variation of the rotor’s upstream velocity on the resource assessment and device performance are not clear since this situation is not typical.To investigate the spatial variation of the upstream velocity two ADCP deployment locations were made. A seabed (upward facing) ADCP as per the current standards and a device mounted (downward facing) ADCP upstream of the rotor plane. Evaluation of the influence of the following three factors were made: i) the ADPs direction repositioning according to prevailing tidal regime, ii) the proximity of the sensors to the sea-surface, and iii) the proximity of the sensors to the TEC.These three factors will be evaluated using ADP datasets collected at Strangford narrows during the VATTS project. The datasets were obtained at approximately the same location of the TEC operation. They enable the study of the following scenarios 1) incoming flow to the rotors during operation, 2) incoming flow on undisturbed conditions (no turbine operation), and 3) a harmonic analysis prediction of the undisturbed incoming flow, which solely captures the tidal-driven flow.The investigation of these three scenarios will provide a better understanding on the rotor’s upstream flow spatial variation, and the influence of the device’s proximity and near sea surface conditions on the mean flow. These findings would benefit developers of alternative TEC designs that operate near the sea-surface.

Occurrence, spatiotemporal distribution patterns,partitioning and risk assessments of multiple pesticide residues in typical estuarine water environments in eastern China

The low terrain and the prosperous agriculture in the east of China, have caused the accumulation of pesticide residues in the estuaries. Therefore, this study analyzed the spatiotemporal distribution and partition tendency of 106 pesticides based on their abundance, frequencies, and concentrations in the aquatic environment of 16 river estuaries in 7 major basins in the eastern China by using solid-phase extraction (SPE) with high-performance liquid chromatography tandem mass spectrometry (HPLC‒MS/MS) and gas chromatography tandem mass spectrometry (GC‒MS/MS). In addition, potential risk of multiple pesticides was also evaluated. The results showed that herbicides were the dominant pesticide type, while triazines were the predominate substance group of pesticide. In addition, triadimenol, vinclozolin, diethylatrazine, prometryn, thiamethoxam, atrazine, and metalachlor were the major pesticides in the water, while prometryn, metalachlor, and atrazine were the main pesticides in the sediment. The average total concentration of pesticide was 751.15 ng/L in the dry season, 651.17 ng/L in the wet season, and 617.37 ng/L in the normal season, respectively. The estuaries of the Huai River Basin, the Yangtze River Basin, the Hai River Basin, and the Yellow River Basin have been affected by the low pollution treatment efficiency, weak infrastructure, and agricultural/non-agricultural activities in eastern China, resulting in relatively serious pesticide pollution. The estuaries of Huaihe River, Yangtze River, Xiaoqing River, and Luanhe River had large pesticide abundance and comparatively severe pesticide pollution, while the estuaries of Tuhai River and Haihe River had heavy pesticide contamination in the sediment, which might be induced by historical sedimentary factors. The log KOC values showed that except for thioketone, other pesticides were relatively stable due to the adsorption by sediment. The ecological risk assessment results indicated that insecticides had a high risk. Teenagers were the most severely affected by the noncarcinogenic risk of pesticides, while adults were mostly affected by the carcinogenic risk of pesticides. Therefore, pesticide hazards in the water environment of estuaries in eastern China needs to be further close supervision.

Growth of Bacillus spp. Isolated From Nipah Worm Intestine (Namalycastis rhodochorde) With Different Combination of pH and Salinity

Bacillus spp. (NrLtF1, NrLtF5, and NrLtG2) isolated from the digestive tract of the Nypa palm worm (Namalycastis rhodochorde) were assumed to have growth characteristics according to the habitat conditions of the nypa worm. Nypa worms live in estuary environments that are affected by seawater intrusion. Salinity and pH are two environmental factors affecting gastrointestinal isolates' growth from nypa palm worms. This study aimed to determine the effect of pH and salinity on the media on growth patterns and determine the optimum combination of pH and salinity for Bacillus spp. The study was conducted using a spectrophotometry method using a microplate reader with Glucose Yeast Peptone (GYP) as a medium for bacterial growth. The medium's pH and salinity values were adjusted by adding 5M NaOH and 5M HCl to obtain pH values of 4, 5, 6, and 7, respectively. Addition of NaCl to the growing medium to get salinity values of 5%, 10%, and 15%. Optimization was determined by incubation at a density of 595 nm at a temperature of 31oC for 24 hours. The results showed that Bacillus spp. could grow well at pH 6 with a salinity value of 15%, pH 6 with a salinity value of 10%, and pH 6 with a salinity value of 5% based on contour plot design. The Optimum growth of Bacillus spp. with pH and salinity expects to be a reference for developing feed products based on indigenous nypa palm worms.

Calcium Sulfate Nanoparticles in Surface Sediments of Lingding Bay of the Pearl River Estuary, China: Implications for the Nonclassical Crystallization Pathway of Gypsum in the Natural Estuary Environment

The mechanism of the nonclassical crystallization pathway of calcium sulfate dihydrate (gypsum) with calcium sulfate hemihydrate (bassanite) as a precursor has been considered in many studies. However, studies on the crystallization of gypsum in natural environments have rarely been reported, especially with regard to natural estuaries, which are one of the most important precipitation environments for calcium sulfate. Here, surface sediments (0–5 cm) of Lingding Bay of the Pearl River Estuary in China were sampled and analyzed. X-ray powder diffraction (XRD) analysis showed that calcium sulfate in the surface sediments mainly existed in the form of gypsum. In high-resolution transmission electron microscopy (HR-TEM) analysis, calcium sulfate nanoparticles were observed in the surface sediments. These particles mainly included spherical calcium sulfate nanoparticles (diameter ranging from 10–50 nm) and bassanite nanorod clusters (sizes ranging from 30 nm × 150 nm to 100 nm × 650 nm), and their main elements included O, S and Ca, with small amounts of N, Si, Na and Mg. The bassanite nanorods self-assembled into aggregates primarily co-oriented along the c axis (i.e., [001] direction). In epitaxial growth into larger bassanite nanorods (100 nm × 650 nm), the crystal form of gypsum could be observed. Based on the observations and analyses, we proposed that the crystallization of gypsum in surface sediments of the natural estuary environment could occur through the nonclassical crystallization pathway. In this pathway, bassanite nanoparticles and nanorods appear as precursors (nanoscale precursors), grow via self-assembly, and are finally transformed into gypsum. This work provided evidence supporting and enhancing the understanding of the crystallization pathway of calcium sulfate phases in the natural estuary environment. Furthermore, the interactions between calcium sulfate nanoparticles and the natural estuary environment were examined.

Microplastic Contamination in the Coastal Environment: A Case Study from the Mae Klong Estuary, Samut Songkhram

The mangrove sediment from the Mae Klong River (MK) and Klong Khon Canal (KK) revealed the microplastics contamination in the Mae Klong Estuary environment. Microplastic analyses were analyzed by using ZnCl2 density separation and H2O2 digestion process. The average concentrations of microplastic was 580 and 1690 items kg-1 dry weight in the samples from MK and KK. MK sediment contained more coarse grain than KK sediment. The lower microplastic concentration in MK was mainly related to the runoff through the sea. In contrast, the abundant microplastic in KK was possibly caused by the low transportation energy of sediment in the tidal flat that associated with deposition of fine grain. In addition, the microplastics were mainly polyester fiber originating from laundry processes or transport from the vicinity. The contamination in mangrove sediment in this study may encourage communities and the government to be more aware of waste management.

Community structure characteristics of ichthyoplankton during the water-sediment regulation scheme in the Yellow River in 2020 and 2021

The Water-Sediment Regulation Scheme (WSRS) will deliver large amounts of water and sand to the Yellow River basin within a short period of time. This will significantly change the physicochemical environment of the Yellow River estuary and the surrounding marine ecosystem. Its effects on the spatial and temporal distribution patterns of ichthyoplankton are still unknown. In this study, six surface horizontal trawl surveys of ichthyoplankton were conducted during the WSRS in 2020 and 2021 using plankton nets. The results were as follows: (1) the estuarine sedentary fish Cynoglossus joyeri was the main species controlling the succession pattern of summer ichthyoplankton communities in the Yellow River estuary. (2) The WSRS influenced the ichthyoplankton community structure by changing the runoff, salinity, and suspension environment in the estuary. (3) The northern and southeastern parts of the estuary near Laizhou Bay were the main aggregation areas of the ichthyoplankton community.