Article Details: Received: 2020-11-30 | Accepted: 2020-12-09 | Available online: 2021-06-30 https://doi.org/10.15414/afz.2021.24.02.117-123 Multi-environment trials were conducted in two locations (Algiers and Setif) during two crop seasons in order to assess the responses of 17 genotype of barley (Hordeum vulgare L.) by evaluation of genotype-by-environment interactions (GEI) on grain yield and determine the stable genotypes. Results showed significant (p <0.001) effects of environment and genotypes and their interaction on grain yield. The genotypes had different behavior conducting to yield variation in the tested locations. So, selection could consider a specific adaptation of the genotypes and their yield stability. The Additive main effects and multiplicative interaction analysis is a useful tool allowing to explore important information on the obtained results; it revealed that ‘Plaisant/ charan01’ is the most stable genotype followed by ‘Barberousse’ and ‘Barberousse/Chorokhod’, while ‘Begonia’ and ‘Plaisant’ were unstable with specific adaptation to Setif location during 2018/19. the cultivar ‘Express’ presented a high productivity. Keywords: AMMI analysis, barley, genotype by environment interaction, grain yield, stability References Abdipur, M. & Vaezi, B. (2014). Analysis of the genotype-by-environment interaction of winter barley tested in the rain-fed regions of Iran by AMMi adjustment. Bulgarian Journal of Agricultural Science, 20(2), 421–427. https://www.agrojournal.org/20/02-27.html Chalak, L. et al. (2015). Performance of 50 Lebanese barley landraces (Hordeum vulgare L. subsp. vulgare) in two locations under rainfed conditions. 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