Entrainment Factor Research Articles

A Balanced Diet Is Necessary for Proper Entrainment Signals of the Mouse Liver Clock

BackgroundThe peripheral circadian clock in mice is entrained not only by light-dark cycles but also by daily restricted feeding schedules. Behavioral and cell culture experiments suggest an increase in glucose level as a factor in such feeding-induced entrainment. For application of feeding-induced entrainment in humans, nutrient content and dietary variations should be considered.Principal FindingTo elucidate the food composition necessary for dietary entrainment, we examined whether complete or partial substitution of dietary nutrients affected phase shifts in liver clocks of mice. Compared with fasting mice or ad libitum fed mice, the liver bioluminescence rhythm advanced by 3–4 h on the middle day in Per2::luciferase knock-in mice that were administered a standard mouse diet, i.e. AIN-93M formula [0.6–0.85 g/10 g mouse BW] (composition: 14% casein, 47% cornstarch, 15% gelatinized cornstarch, 10% sugar, 4% soybean oil, and 10% other [fiber, vitamins, minerals, etc.]), for 2 days. When each nutrient was tested alone (100% nutrient), an insignificant weak phase advance was found to be induced by cornstarch and soybean oil, but almost no phase advance was induced by gelatinized cornstarch, high-amylose cornstarch, glucose, sucrose, or casein. A combination of glucose and casein without oil, vitamin, or fiber caused a significant phase advance. When cornstarch in AIN-93M was substituted with glucose, sucrose, fructose, polydextrose, high-amylose cornstarch, or gelatinized cornstarch, the amplitude of phase advance paralleled the increase in blood glucose concentration.ConclusionsOur results strongly suggest the following: (1) balanced diets containing carbohydrates/sugars and proteins are good for restricted feeding-induced entrainment of the peripheral circadian clock and (2) a balanced diet that increases blood glucose, but not by sugar alone, is suitable for entrainment. These findings may assist in the development of dietary recommendations for on-board meals served to air travelers and shift workers to reduce jet lag-like symptoms.

The entrainment factor in froth flotation: Model for particle size and other operating parameter effects

Accurately estimating entrainment is crucial when predicting flotation performance as it is essential for determining the concentrate grade achieved. It has been found previously that the amount of gangue entrained is proportional to the water recovery; this proportionality is referred to as the entrainment factor. Experimentally it has been found that entrainment is a strong function of particle size, as well as being dependent on other cell operating parameters such as froth depth and air rate. A simplified theoretical model for entrainment is developed which includes the effects of liquid motion and content, particle settling and particle dispersion. First, a detailed one-dimensional differential model for the entrainment factor is developed and solved numerically. Thereafter, a simplified analytical expression for the entrainment factor is produced which is a good approximation to the more detailed one-dimensional model. Both these models are shown to predict closely experimental trends for entrainment as a function of particle size and froth depth.

A correlation model of thermal entrainment factor for air curtain in a vertical open display cabinet

Thermal entrainment factor (TEF) is an important parameter to describe the cold preservation performance of a display cabinet. In order to calculate the TEF of a display cabinet rapidly and accurately, the method of establishing a correlation model based on computational fluid dynamics (CFD) simulation results is presented and the correlation model of TEF for typical single and double air curtain in a vertical open display cabinet is developed in this paper. The TEF of display cabinet predicted by the correlation model gives a maximum deviation of −9.4% and a mean deviation of −0.1% compared with the published experimental data. In addition, the correlation model can be used to calculate the air curtain return temperature, showing a maximum deviation of 0.9 °C and an average deviation of 0.1 °C compared with the published experimental data.

Direct measurement of entrainment in large flotation cells

A key response variable to describe the metallurgical performance in a flotation cell is the concentrate grade. The mineral feed characteristics and conditioning determine the grade of the particles attached directly to the surfaces of the bubbles by true flotation, while the operating conditions, such as gas rate, bubble size, froth depth and others, determine the amount of gangue recovered by entrainment, which finally decreases the concentrate grade. In this paper, the recovery of liquid and solids by entrainment was evaluated by direct measurement of the fraction of liquid and solids reported to the concentrate in a 130 m 3 mechanical flotation cell. The liquid and solids entrainment, per size classes (+ 150; − 150 + 45; − 45 µm), was measured by the radioactive tracer technique. The procedure consisted of introducing a tracer impulse at the cell feed entrance. The tracer time response was monitored on-line at the concentrate overflow and at the tailings discharge. Also, in order to obtain the quantitative distribution of the feed, samples were taken periodically from the concentrate and tailings streams, for a period of 4 residence times, during the tracer tests. This allowed the quantification of the mass of tracer reporting to both streams. Experimental results confirmed that solids entrainment with the froth was strongly dependent on particle size, and that the entrainment factor (EF = solid/water recovery ratio) was similar to that reported for smaller size cells under similar operations conditions.

Ocean Sequestration of Carbon Dioxide: Modeling the Deep Ocean Release of a Dense Emulsion of Liquid CO2-in-Water Stabilized by Pulverized Limestone Particles

The release into the deep ocean of an emulsion of liquid carbon dioxide-in-seawater stabilized by fine particles of pulverized limestone (CaCO3) is modeled. The emulsion is denser than seawater, hence, it will sink deeper from the injection point, increasing the sequestration period. Also, the presence of CaCO3 will partially buffer the carbonic acid that results when the emulsion eventually disintegrates. The distance that the plume sinks depends on the density stratification of the ocean, the amount of the released emulsion, and the entrainment factor. When released into the open ocean, a plume containing the CO2 output of a 1000 MW(el) coal-fired power plant will typically sink hundreds of meters below the injection point. When released from a pipe into a valley on the continental shelf, the plume will sink about twice as far because of the limited entrainment of ambient seawater when the plume flows along the valley. A practical system is described involving a static mixer for the in situ creation of the CO2/seawater/pulverized limestone emulsion. The creation of the emulsion requires significant amounts of pulverized limestone, on the order of 0.5 tons per ton of liquid CO2. That increases the cost of ocean sequestration by about $13/ ton of CO2 sequestered. However, the additional cost may be compensated by the savings in transportation costs to greater depth, and because the release of an emulsion will not acidify the seawater around the release point.

Bidirectional CLOCK/BMAL1-dependent circadian gene regulation by retinoic acid in vitro

A central circadian clock located in the suprachiasmatic nucleus (SCN) of the mammalian hypothalamus entrains peripheral clocks through both neural and humoral factors. Although candidates for entrainment factors have been described, their details remain obscure. Here, we screened ligands for nuclear receptors that affect CLOCK/BMAL1-dependent transactivation of the mouse Period1 ( mPer1) gene in NIH3T3 cells. We found that retinoic acids (RAs) significantly up-regulate mPer1 expression in an E-box-dependent manner. We also found that RAs up-regulate the expression of other E-box-dependent circadian genes such as mPer2, arginine vasopressin ( mAVP), and peroxisome proliferator-activated receptor α ( mPPARα). Surprisingly, the effect of RAs on CLOCK/BMAL1 (E-box)-dependent mRNA expression was bidirectional and depended on the presence of exogenous retinoic acid receptor α (RARα). These results suggest that RAs regulate the CLOCK/BMAL1-dependent transcription of circadian genes in a complex manner.

Moon orientation in sandhoppers: effects of lighting treatments on the persistence of orientation ability

The moon orientation rhythm persists in sandhoppers removed from environmental entrainments relative to moon periodicity. In order to investigate the underlying mechanism, the crustacean amphipods Talorchestia capensis and Talitrus saltator were collected at new moon and exposed in the laboratory to an artificial light during the day, but to a variety of lighting treatments during the night: (1) continuous dark, (2) dim light in phase with the natural moon, (3) continuous dim light. The animals were tested under the moon 9–21 days later by using a new type of arena. The ability to orientate in a direction perpendicular to the shore persisted in sandhoppers under treatments 1 and 2, but not under treatment 3. A disturbance due to a phototactic tendency in Talorchestia was also observed in animals captured on the day of the test when exposed to unnatural lighting, but not when they were kept under natural light. The present findings show that the timing mechanism allowing compensation for changes in the moon’s position also persists in animals that have been long removed from entraining factors. The dependence of this orientation ability on nocturnal lighting and the disturbing effect of sudden changes in light intensity support the idea that the phasal lighting of the moon, and perhaps sunrise and sunset act as resetting factors for the moon’s orientation rhythm.

The in vitro real-time oscillation monitoring system identifies potential entrainment factors for circadian clocks

BackgroundCircadian rhythms are endogenous, self-sustained oscillations with approximately 24-hr rhythmicity that are manifested in various physiological and metabolic processes. The circadian organization of these processes in mammals is governed by the master oscillator within the suprachiasmatic nuclei (SCN) of the hypothalamus. Recent findings revealed that circadian oscillators exist in most organs, tissues, and even in immortalized cells, and that the oscillators in peripheral tissues are likely to be coordinated by SCN, the master oscillator. Some candidates for endogenous entrainment factors have sporadically been reported, however, their details remain mainly obscure.ResultsWe developed the in vitro real-time oscillation monitoring system (IV-ROMS) by measuring the activity of luciferase coupled to the oscillatory gene promoter using photomultiplier tubes and applied this system to screen and identify factors able to influence circadian rhythmicity. Using this IV-ROMS as the primary screening of entrainment factors for circadian clocks, we identified 12 candidates as the potential entrainment factor in a total of 299 peptides and bioactive lipids. Among them, four candidates (endothelin-1, all-trans retinoic acid, 9-cis retinoic acid, and 13-cis retinoic acid) have already been reported as the entrainment factors in vivo and in vitro. We demonstrated that one of the novel candidates, 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2), a natural ligand of the peroxisome proliferator-activated receptor-γ (PPAR-γ), triggers the rhythmic expression of endogenous clock genes in NIH3T3 cells. Furthermore, we showed that 15d-PGJ2 transiently induces Cry1, Cry2, and Rorα mRNA expressions and that 15d-PGJ2-induced entrainment signaling pathway is PPAR-γ – and MAPKs (ERK, JNK, p38MAPK)-independent.ConclusionHere, we identified 15d-PGJ2 as an entrainment factor in vitro. Using our developed IV-ROMS to screen 299 compounds, we found eight novel and four known molecules to be potential entrainment factors for circadian clocks, indicating that this assay system is a powerful and useful tool in initial screenings.

Photoperiod regulates clock gene rhythms in the ovine liver

To investigate the photoperiodic entrainment of peripheral rhythms in ruminants, we studied the expression of clock genes in the liver in the highly seasonal Soay sheep. Animals were kept under long (LD 16:8) or short photoperiod (LD 8:16). Daily rhythms in locomotor activity were recorded, and blood concentrations of melatonin and cortisol were measured by RIA. Per2, Bmal1, and Cry1 gene expression was determined by Northern blot analyses using ovine RNA probes in liver collected every 4 h for 24 h. Liver Per2 and Bmal1, but not Cry1, expression was rhythmic in all treatments. Under long days, peak Per2 expression occurred at end of the night with a similar timing to Bmal1, whereas, under short days the Per2 maximum was in the early night with an inverse pattern to Bmal1. There was a photoperiod × time interaction for only Per2 ( P < 0.001). The 24-h pattern in plasma cortisol matched the observed phasing of Per2 expression, suggesting that it may act as an endocrine entraining factor. The clock gene rhythms in the peripheral tissues were different in timing compared with the ovine suprachiasmatic nucleus (SCN, central pacemaker) and pars tuberalis (melatonin target tissue), and the hepatic rhythms were of lower amplitude compared with photoperiodic rodents. Thus, there are likely to be important species differences in the way the central and peripheral clockwork encodes external photoperiod.

Voltage‐dependent calcium entry underlies propagation of slow waves in canine gastric antrum

Electrical slow waves in gastrointestinal (GI) muscles are generated by interstitial cells of Cajal (ICC), and these events actively propagate through networks of ICC within the walls of GI organs. The mechanism by which spontaneously active pacemaker sites throughout ICC networks are entrained to produce orderly propagation of slow waves is unresolved. A three-chambered partition bath was used to test the effects of agents that affect metabolism, membrane potential and voltage-dependent Ca(2+) entry on slow wave propagation in canine antral smooth muscle strips. Slow waves evoked by electrical field stimulation actively propagated from end to end of antral muscle strips with a constant latency between two points of recording. When the central chamber of the bath was perfused with low-temperature solutions, mitochondrial inhibitors, reduced extracellular Ca(2+) or blockers of voltage-dependent Ca(2+) channels, active propagation failed. Depolarization or hyperpolarization of the tissue within the central chamber also blocked propagation. Blockade of propagation by reduced extracellular Ca(2+) and inhibitors of dihydropyridine-resistant Ca(2+) channels suggests that voltage-dependent Ca(2+) entry may be the 'entrainment factor' that facilitates active propagation of slow waves in the gastric antrum.

Experimental study of the performance of single-band air curtains for a multi-deck refrigerated display cabinet

Experiments were conducted to study the effects of several important factors on the performance of a refrigerated display cabinet, including ambient air temperature, indoor relative humidity, ambient air flow, air supply velocity, air flow from perforated back panels and night covers. Both inside temperature distribution and heat load were investigated. Furthermore, the thermal entrainment factor is correlated with Reynolds number and Richardson number. The results show the thermal entrainment tends to increase with increasing momentum force but decrease when the gravitational force is dominant.

Circadian Rhythms in Infants

After completing this article, readers should be able to: 1. Describe how circadian rhythms are generated. 2. Describe how lighting influences the circadian system. 3. Delineate the period during which the origin of circadian rhythms develops. 4. Describe the relationship between maternal circadian rhythms and the development of circadian rhythms in infants. 5. Characterize the potential sequelae of impaired fetal and early neonatal circadian rhythms related to sleep patterns. 6. Describe beneficial lighting patterns for preterm infants in the neonatal intensive care nursery. Circadian rhythms are endogenously generated rhythms that have a period length of about 24 hours. Evidence gathered over the past decade indicates that the circadian timing system develops prenatally, with the suprachiasmatic nuclei (SCN) in the anterior hypothalamus, the site of a circadian clock, present by mid-gestation in primates. Recent evidence also shows that the circadian system of primate infants is responsive to light at very early stages (as early as 25 to 28 weeks’ gestation in humans) and that low-intensity lighting can regulate the developing clock. After birth, circadian system outputs mature progressively, with rhythms in sleep-wake cycles, body temperature, and hormone production generally developing between 1 and 3 months of age. The importance of light in regulation of circadian rhythm in infants is highlighted by the early establishment of rest-activity patterns that are in phase with the 24-hour light-dark cycle in preterm infants exposed to low-intensity cycled lighting. With the continued elucidation of circadian system development and influences on human physiology and illness, it is anticipated that consideration of circadian biology will become an increasingly important component of neonatal care. ### The Circadian Timing System Notable examples of circadian rhythms include the sleep-wake cycle and daily rhythms in body temperature and hormone production. Circadian rhythms are also involved in the pathogenesis of illnesses, such as reactive airway disease (eg, asthma) and myocardial infarction. The system responsible …

Sleep-wake states and their regulatory mechanisms throughout early human development

The emergence of sleep states is one of the most significant aspects of development. Descriptions of both neonatal and late fetal behavior and studies on the organization of sleep have shown that fetus and newborns exhibit spontaneously discrete and cyclic patterns of active sleep (AS) and quiet sleep (QS). Human fetuses and neonates sleep most of their life, and AS is the prevailing state even during the first postnatal months. Several hypotheses to explain central nervous system development consider that AS is the expression of a basic activation program for the central nervous system that increases the functional competence of neurons, circuits, and complex patterns before the organism is called on to use them. Current results indicate the maturation of QS not only coincides with the formation of thalamocortical and intracortical patterns of innervation and periods of heightened synaptogenesis, since this sleep state is also associated with important processes in synaptic remodeling. In fact, several studies suggest that the information acquired during wakefulness is further processed during AS and QS. This article reviews the processes involved in the timing of both AS/QS and sleep/wake alternating patterns throughout early human development. A growing body of evidence indicates that the duration of unmodulated parental care and noncircadian environmental conditions may be detrimental for the establishment of these basic rhythmicities. As a consequence, alterations in parental/environmental entraining factors may well contribute to disturb sleep and feeding commonly experienced by preterm infants. Further knowledge on the early establishment of sleep-wake states regulatory mechanisms is needed to improve modalities for appropriate stimulation in the developing human being.

Changes in L1 and NCAM expression in the rat suprachiasmatic nucleus during growth and after orbital enucleation

Mammals possess a master circadian clock in the hypothalamic suprachiasmatic nucleus (SCN). In order to clarify the roles of the L1 adhesion molecule (L1) and neural cell adhesion molecule (NCAM), both members of the immunoglobulin superfamily, in the organization of the clock core, changes in the expression of these molecules in the SCN during the growth of rats were examined by immunohistochemistry. On postnatal day 7, L1 and NCAM were chiefly expressed in the region surrounding the SCN, but not in the SCN itself. In subsequent weeks, however, expression of both molecules shifted predominately to the SCN. This change seemed to parallel immunoreactivity increases in the SCN of synaptotagmin, a synapse marker, and of phosphotyrosine, a possible factor in the photic entrainment of the SCN clock. To further elucidate the roles of the L1 and NCAM adhesion molecules in the formation and maintenance of retinal neural projection into the SCN, the effects of orbital enucleation on their expression in the SCN were examined. L1 expression decreased on days 1 and 2 after the operation, in parallel with reductions in the tyrosine phosphorylation of several proteins, but recovered to the control level by the second week. In contrast, the expression of NCAM showed little change following orbital enucleation. These results suggest that L1 and NCAM are involved in the morphological organization of the SCN during the developmental stage, and that expression of L1 also contributes to the formation of the SCN network in a manner that is dependent on the retinal neural input to it.

Transport of salts and micron-sized particles entrained from a boiling water pool

The entrainment of soluble (KI, CsI) and non-soluble (Al 2O 3) substances through droplets, which are produced by disintegrating steam bubbles at the surface of a boiling water pool, is determined in a pilot-scale facility. Integral measurements are conducted at steady-state conditions in an atmosphere of either pure steam or an air–steam mixture. The ratio of the entrained liquid mass flow and the gas mass flow through the pool, the entrainment factor, is determined for air–steam ratios between 0 and 0.47 kg/kg in the gas atmosphere and at constant total pressures between 2 and 6 bar. The influence of the vertical temperature profile in the gas atmosphere on the convective velocity field is demonstrated by phase Doppler anemometry and particle image velocimetry measurements at a location 2.1 m above the pool surface. The influences of nucleation and natural convection are demonstrated during slow de-pressurization of the facility at rates below 420 Pa/s.

CHARACTERIZING FREE-LIVING LIGHT EXPOSURE IN A WORK ENVIRONMENT USING A WRIST-WORN LIGHT MONITOR

PURPOSE: The purpose of this study was to pilot the use of a new wrist-worn light monitoring device (MTI AM 7164–2.3, Manufacturing Technology, Inc., Fort Walton Beach, FL) to document that indoor lighting for a particular day-shift work environment could serve as the primary light exposure (LE) dosage in healthy free-living humans. METHODS: Eleven employees (8F, 3M) of a local hospital volunteered to wear a light monitoring device on the non-dominant wrist during all waking hours for an entire workweek (Monday-Friday, August-September). The light monitors, recording average light intensity (units of lux) over 1-min epochs, were downloaded to a computer and analyzed using a custom Visual Basic program. The raw data was summarized as the number of mins and the dose of LE (mins of exposure × mid-point LE category) within six LE categories (< 1, 1–100, 101–200, 201–500, 501–1000, > 1000 lux). The LE variables, summarized across both work and non-work environment time periods (tracked by self-report), were analyzed using a 3-Factor RM ANOVA. RESULTS: LE duration was significantly greater in the LE categories common to the non-work environment (Mean ± SE:594 ± 16 mins in < 1 lux, and 168 ± 22 mins in 1–100 lux; p < 0.01). LE dose, however, was greatest (p < 0.01) in the 101–200 (350 ± 23 lux-hrs) and 201–500 lux (1406 ± 152 lux-hrs) categories, which was the light intensity range most common to the hospital environment. CONCLUSIONS: The MTI light monitor appeared to provide a reasonable means for evaluating free-living light exposure in the work environment. These data support the premise of others that long term exposure to dim indoor light intensities (i.e. the dose and not time of light exposure) commonly experienced within a variety of work environments may werve as the primary entrainment factor for physiological and behavioral processes following a circadian rhythm.

Characterizing free-living light exposure using a wrist-worn light monitor

It was the purpose of this study to pilot the use of a new wrist-worn light monitoring device to document that indoor lighting for a particular day-shift work environment could serve as the primary light exposure dosage in healthy free-living humans. Twelve employees of a local hospital volunteered to wear a wrist-worn light monitor during all waking hours for an entire workweek (Monday–Friday). Light data were analyzed (3-Factor RM ANOVA) for the dose of light exposure (minutes of exposure x light intensity) within six light intensity categories (<1, 1–100, 101–200, 201–500, 501–1000, >1000 lx) relative to time spent within their work environment and all other time of the day. The greatest dose of exposure occurred within the 201–500 lx range during the subjects’ work shift. These data support the premise of others that long term exposure to dim indoor light intensities commonly experienced within a variety of work environments may serve as the primary entrainment factor for physiological and behavioral processes following a circadian rhythm.

A circadian system model with feedback of cross-correlation between sleep-wake rhythm and oscillator.

Recent findings suggest that social entrainment factors work on an oscillator other than the temperature and melatonin rhythms. This indicates that there is a feedback loop affected on oscillators. In the present study, a circadian system model, including mutually coupled two oscillators and sleep-wake rhythm, is constructed, where cross-correlation between a sleep-wake rhythm and an oscillator is set to modify the coupling from one oscillator to the other. Based on the model simulation, a mechanism underlying delayed sleep phase syndrome is elucidated by the reduction in the cross-correlation resulting from dissociation between the delayed sleep phase and the oscillator.

Effect of aging on circadian activity in gray mouse lemurs

Microcebus murinus s a very photoperiod-dependent primate with a potentially extended longevity (13 years). Reduction of artificial seasonal cycles allows acceleration of the aging process. Under these conditions, age is defined according to the number of seasonal cycles. We conducted experiments in order to assess the effects of aging upon (1) the main parameters (period: τ duration: α) of the circadian activity–rest rhythm; and (2) the plasticity of the response to light, which is the main entraining factor of the internal clock. We studied the evolution of τ and α through two types of experiments: a transverse one comparing 36 males of various ages (1–13 seasonal cycles) and a longitudinal one following 2 pairs of males from the same litter (one from each pair was maintained under natural cycle while the other was submitted to a shortened cycle) over 54 months. Results from transverse experiments demonstrated no statistical difference in τ and α with age except in 4 senescent (>10 cycles) subjects in which these two parameters were decreased. Longitudinal experiments confirmed this tendency. The plasticity of responses to light, resynchronization after a shift of the day–night cycle, or shift of activity onset after presentation of a light pulse at various circadian times was unaffected by aging. Taken together, the data demonstrate that the parameters of the circadian activity–rest rhythm remain stable over a long span of life and/or that light remains a powerful entraining parameter even in very old individuals.

ON THE USE OF VIBRATION AND ELECTRIC FIELDS TO FLUIDIZE AND DISPERSE PARTICULATE MATTER

ABSTRACT Particulate matter is a statistical ensemble of interacting, non-identical particles. The fluidization and dispersal of dust is considered here from kinetic arguments and experiment. It is concluded that fluidization and dispersal of dust are independent physical processes arising from the action of vibration and steady stresses, respectively. Although the transition or correlation function for particulate systems is not as sharply defined as for the ideal-gas case, its general character is analogous with that for a condensing phase-transition with its critical condition broadened by the distribution of binding energies between the particles. The ratio of the net steady stress on the particles to the vibrational energy is the controlling factor in particle capture and entrainment.