Entire Margin Research Articles

Quality control standardization of the leaves and root of Landolphia owariensis (Apocynaceae)

The leaves and roots of Landolphia owariensis have received much attention for their incredible ethnomedicinal uses and are widely commercialized as aphrodisiac, anti-infective and anti-inflammatory agents in West Africa. A systematic evaluation of the leaves and roots was carried out in order to set standards for its identification and quality control. The organoleptic, macro-morphology and micromorphology of whole and powdered leaves and roots were carried out. Physicochemical, phytochemical, fluorescence analysis and preliminary HR-LC/MS analysis were also performed.From organoleptic and macromorphological studies, the leaf was identified to be ovate in shape with an acuminate apex, symmetrical base, entire margin and reticulate venation. Characteristic microscopic features of the leaf lamina, petiole and midrib include the presence of hypostomatic anomocytic stomata, wavy walled epidermal cells, a concentric vascular bundle displaying xylem and phloem cells, unicellular clothing trichomes and secretory cells. Prismatic calcium oxalate crystals, broken stomata, stone cells, pitted vessels, fibers, cork cells and unicellular clothing trichomes are found in the leaf and root powder. Various solvent soluble extractives, ash content, moisture content, pH and fluorescence characteristics were determined. Tannins, saponins, triterpenes and alkaloids were the major phytoconstituents identified. The essential diagnostic attributes of L. owariensis established in this study are useful for the authentication and quality control of the plant.

Phenotypic characterization and presumptive identification of bacterial isolates from moribund Nile tilapia (Oreochromis niloticus L.)

The general objective of this study was to isolate, characterize and presumptively identify bacterial isolates from moribund Nile tilapia (Oreochromis niloticus L.). The rigid sorting of the bacterial colonies based on cultural characteristics has resulted to three different bacteria. Colony A and colony B shared the same cultural characteristics – round with raised margin, small in size, and with entire margin, smooth texture and convex elevation. Colony C was entirely different in shape, margin, texture and elevation. The three bacterial colonies failed to grow in McConkey Agar but luxuriously grow in Staphylococcus Selective Agar, therefore, colony A and colony B were presumptively identified under the genus Staphylococcus. Colony A and colony B were Gram-positive, spherical in shape, positive to catalase test, and negative to several tests such as citric acid utilization, urea hydrolysis and phenylalanine deamination.

PHARMACOGNOSTIC STANDARDIZATION OF JACARANDA MIMOSIFOLIA LEAVES AND STEM BARK

pharmacognostic standardization is a basic criterion to assure authenticity and quality of any medicinal plant. In the present study micromorphological examinations along with proximate analysis, fluorescence and elemental analysis of Jacaranda mimosifolia leaves and stem bark were carried out. Morphological examination indicated compound, bipinnate and alternately arranged leaves having several pairs of leaflets. The leaflets were oblong ellipsoidal in shape with parallel venation, acute apex and entire margin. The stem bark was concavely curved, greyish brown colored with conical shaped buds on the surface. Microscopic examination of leaves presented dorsiventral lamina, unicellular pointed trichomes, vascular bundle, collenchyma tissues and calcium oxalate crystals while bark indicated cork, stone cells, lignified fibres, calcium oxalate prisms, starch grains and medullary rays. Elemental analysis revealed the presence of aluminum, chlorides and iron in leaves and bark of Jacaranda mimosifolia. The present study will assist as an important source of information in identification and further investigation of this plant.

ISOLATION AND IDENTIFICATION OF BRENNERIA NIGRIFLUENS AS CAUSAL AGENT OF BARK CANKER DISEASE ON WALNUT IN IRAQ

Bark canker disease has recently found in Sulaimani province. Disease incidence reached to 17.2% in Tawella. Bacterial isolates were identified by their morphological and biochemical characteristics using the API 20E system. Fifty-one and half percent of the isolates were identified as Brenneria nigrifluens and 36.3% as Pantoea spp. B. nigrifluens isolates formed circular colonies with entire margins and creamy color on nutrient agar. Biochemical tests classify B. nigrifluens isolates into seven groups. Vitek GN system further used to confirm the identification. The isolates produced necrotic lesion of different size on artificial inoculated walnut branches, but no symptoms on detached leaves. Isolates 22, 28 and 31 induced typical symptoms on two years old seedlings. B. nigrifluens successfully re-isolated from the inoculated seedlings. All B. nigrifluens isolates were resistant to erythromycin and cephalexin, most of them (16/17) were resistant to ampicillin, (13/17) resistant to vancomycin, (12/17) resistant to rifampin and amikacin; while (10/17) were moderately resistant to penicillin; (7/17) were moderately susceptible to streptomycin and (6/17) to gentamicin. Chloramphenicol, tobramycin, and tetracycline showed high efficiency in bacterial growth inhibition. Minimum inhibitory and minimum bactericidal concentrations of five chemicals against 17 bacterial isolates showed high efficiency of Kocide in killing 94.1% and inhibiting of 100% of the isolates at 0.31g/L In vitro, followed by Nordox which kill 70.6% and inhibit 76.4% of the isolates at 0.65 and 0.32 g/L respectively. In vivo studies confirm high efficacy of Kocide in disease control and restriction of vertical and horizontal extension of the cankers followed by Nordox.

Два новых вида рода бегония (Begonia L., Begoniaceae) из центрального Лаоса

Begonia comprises at least 1850 species, most of which are local endemics. The greatest genus diversity is observed in tropical zone of America and Asia with particular rich area in Indochinese Peninsula. Meanwhile, the inventory of the genus in this region remains far from acceptable completing. Until recently, only 20 species were documented in Laos. Two more species are described here. Both are local endemics found in small sporadic limestone formations in central Laos. The first species, B. quadripetiolata (expectedly sect. Platycentrum) is endemic of Vientiane province (Vang Vieng district). In morphology of staminate flowers, it allies to B. subhowii described from southeastern Yunnan. From B. subhowii and related B. howii, new species differs in larger leaves, entire leaf margin, leaf petiole quadrate in cross section and very small, caducous inflorescence bracts. Leaf petiole quadrate in cross section, is unique character in the genus. The second species, B. tatianae (sect. Diploclinium) named after its discoverer and grower – Mrs. Tatiana Maisak, allies to B. cladotricha, B. gesneriifolia and B. hinnamnoensis originated from limestone formations associated with Hin Nam No Nature Protected Area (central Laos and Vietnam). From allied species, our plant differs in miniature habit, glabrous leaves above dark green, with large diffuse white spot (with small white crystalloid bodies), few stamens, pistillate flowers normally having 4 tepals and ovary bearing wings of distinctly unequal size. Both described species represent characteristic native element of aboriginal limestone flora of lowland karstic formations in central part of Laos.

Arenibacter amylolyticus sp. nov., an amylase-producing bacterium of the family Flavobacteriaceae isolated from marine water in India.

A novel Gram-stain-negative, curved rod-shaped, 0.5-0.7 µm wide and 3.0-10.0 µm long, non-motile bacterium, designated strain AK53T, was isolated from a 5 m depth water sample collected from the Bay of Bengal, Visakhapatnam, India. Colonies on marine agar were circular, small, dark orange, shiny, smooth, translucent, flat, with an entire margin. The major fatty acids included iso-C15 : 0, iso-C15 : 0 3OH, anteiso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3OH and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c and/or iso-C15 : 0-2OH). Polar lipids included phosphatidylethanolamine and five unidentified lipids. The DNA G+C content of the strain AK53T was found to be 40.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AK53T was closely related to Arenibacter latericius KMM 426T and Arenibacter certesii KMM3941T (pair-wise sequence similarity of 99.17 and 98.89 %, respectively), forming a distinct branch within the genus Arenibacter and clustering with A. latericius. Strain AK53T shared average nucleotide identity (ANIb, based on blast) of 78.07 and 77.44 % with A. latericius JCM 13508T and A. certesii JCM 13507T, respectively. Based on the observed phenotypic, chemotaxonomic characteristics and phylogenetic analysis, strain AK53T is described in this study as representing a novel species in the genus Arenibacter, for which the name Arenibacter amylolyticus sp. nov. is proposed. The type strain of Arenibacter amylolyticus is AK53T (=MTCC 12004T= JCM 19206T=KCTC 62553T).

Sandarakinorhabdus rubra sp. nov., and Sandarakinorhabdus oryzae sp. nov., isolated from oxidized rice paddy soil.

Three Gram-stain-negative, motile or non-motile, rod-shaped, facultatively aerobic strains, designated MO-4T, NP-34 and NM-18T, were isolated from oxidized rice paddy soil in Chungbuk, Republic of Korea. Colonies were circular and convex with entire margins, red in colour on R2A after 3 days at 30 °C. The three strains grew at pH 5.0-10.0 (optimum, pH 8.0), at 15-45 °C (optimum, 30 °C) and at salinities of 0-1.5 % (w/v) NaCl (optimum, 0.4 % NaCl). The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that the three isolates represent members of the genus Sandarakinorhabdus and strains MO-4T and NP-34 were most closely related to Sandarakinorhabdus cyanobacteriorum TH057T (97.7 %) and Sandarakinorhabdus limnophila DSM 17366T (97.1 %). NM-18T showed highest 16S rRNA gene sequence similarities to Sandarakinorhabdus limnophila DSM 17366T (98.7 %) and Sandarakinorhabdus cyanobacteriorum TH057T (96.7 %). Genomic similarities between strains MO-4T and NM-18T and the two type strains of species of the genus Sandarakinorhabdus based on average nucleotide identity and digital DNA-DNA hybridization values were lower than the species delineation thresholds. The major fatty acids were iso-C18 : 1 ω7c and summed feature 3. The DNA G+C contents of strains MO-4T and NM-18T, obtained from genome sequencing data, were 67.6 and 66.6 mol%, respectively. On the basis of these genotypic and phenotypic characteristics, the three strains are assigned to two novel species of the genus Sandarakinorhabdus, for which the names Sandarakinorhabdus rubra sp. nov. (type strain MO-4T =KACC 21378=NBRC 114106) and Sandarakinorhabdus oryzae sp. nov. (type strain NM-18T=KACC 21379=NBRC 113957) are proposed.

Morphological and anatomical studies on some taxa of family Apocynaceae

Apocynaceae is one of the important families that is present all over the world. The aim of this study is to provide some anatomical features of 7 species that represent 7 genera belonging to 4 subfamilies (Apocynoideae, Asclepiadoideae, Periplocoideae and Rauvolfioideae) related to Apocynaceae. The species samples were collected from different areas in Egypt. The study was focus on the morphological and anatomical characters of stems and leaves. The results were recorded in the form of comparison between the examined plants. Most of these plants are perennial herbs as in Cynanchum acutum L. but some are shrubs as in Carissa spinarum L. and trees as in Alstoniascholar is R.Br. The stem is woody in some of the investigated plants as in Alstoniascholaris R.Br. and succulent in some plants as in Pachypodium lamerei. The leaves are simple, spirally, opposite and opposite decussate. Leaves shape are ovate, elliptic, oblong-lanceolate or cordate with entire margin. The vascular bundles in all of the examined plants are bicollateral. From the analysis of all the available data by using the Multi Variate Statistical Package (MVSP) Program it could be stated that Apocynaceaeand Asclepiadaceae are very similar to each other which could be ranked in one large family Apocynaceae. In conclusion, we emphasize the previous recommendations to merge both Apocynaceae and Asclepiadaceae families in one large family, that is Apocynaceae.

Lecanorchis moritae (Orchidaceae, Vanilloideae), a new mycoheterotrophic species from Amami-Oshima Island, Japan, based on morphological and molecular data

A new species of the mycoheterotrophic genus Lecanorchis Blume (Orchidaceae), L. moritae, is described from Amami-Oshima Island, Japan. It is most similar to L. suginoana but differs in having a transversely elliptic (slightly contracted at base) midlobe (vs. semiorbicular, not contracted at base), more triangular with almost entire margins (vs. relatively rounded, denticulate margins) and reddish apices (vs. somewhat whitish apices) and a lip with white and purple hairs (vs. white and lemon yellow hairs) and sparse unicellular papillae scattered on the disc (vs. dense unicellular papillae). An illustration and nuclear ribosomal internal transcribed spacer (nrITS) sequence (i.e., DNA barcode) of the new species are also provided.

Neuropathology of Neuropsychiatric Systemic Lupus Erythematosus (NPSLE)

Neuropathologic alterations of neuropsychiatric systemic lupus erythematosus (NPSLE) are characterized by small vessel changes such as endothelial proliferation, fibrosis, hyalinization, fibrinoid necrosis, and thrombus formation. Pathologic ischemic and hemorrhagic changes. In some instances, vasculitis and perivascular infiltration of inflammatory cells are seen. In the spinal cord, degenerative changes of entire margin of the spinal cord may also be present.

Gymnosiphon philippinensis, a new species of Burmanniaceae from Cebu, Philippines

Gymnosiphon philippinensis is described as a new species of Burmanniaceae from forest over limestone in southern Cebu (Philippines). Among Malesian Gymnosiphon, it is most similar to G. papuanus and G. pauciflorus in having flowers with stamens that are attached in the middle of the floral tube and having outer tepals with entire margins, but these tepals are longer in absolute and relative lengths than those of the aforementioned species. Gymnosiphon philippinensis is the first species of Gymnosiphon reported from the Philippines.

A new species of Pseudobunocephalus Friel, 2008 (Siluriformes: Aspredinidae) from the lower Tocantins and Mearim river drainages, North and Northeast of Brazil.

Pseudobunocephalus timbira, new species, is described from streams of the lower Tocantins and the Mearim river drainages, in North and Northeast of Brazil. Pseudobunocephalus timbira can be distinguished from all congeners by having the second hypobranchial and the third basibranchial cartilaginous (vs. ossified). Additionally, it can be dintinguished from P. lundbergi by the following putative apomorphic features within Pseudobunocephalus: posterolateral process of premaxilla present (vs. absent); bony knobs in dorsal lamina of Weberian apparatus absent (vs. present); distal end of posterior margin of 5th parapophysis not enlarged (vs. enlarged); number of ribs three (vs. four or five) and infraorbital sensory canal entering neurocranium via frontal (vs. via sphenotic). It is distinguished from P. bifidus and P. iheringii by having a gracile body not surpassing 34 mm SL (vs. robust body, reaching up to 59 mm SL, respectively); by having the posterior margin of cranial fontanel concave (vs. posterior margin somewhat straight with parieto-supraoccipital extending anteriorly); by having a conspicuous knobby ornamentation on dorsal surface of skull (vs. skull knobs slightly pronounced); by having Weberian ventral blade of hemal canal opened (vs. closed) and by the absence of serrations on the proximal portion of the anterior margin of pectoral-fin spine (vs. serrations covering entire anterior margin of the pectoral spine). Additionally, it can be distinguished from P. amazonicus, P. rugosus and P. quadriradiatus, by having the posterolateral mental barbel with at least one fleshy lobe located proximally along the posterior margin (vs. posterolateral mental barbel simple, not having fleshy lobes). It also differs from P. amazonicus and P. rugosus by having five branchiostegal rays (vs. four). It also can be distinguished from P. amazonicus by having the contact of hyomandibula cartilage with neurocranium limited to the sphenotic (vs. extending to both sphenotic and pterotic); by having the ventral blade of Weberian apparatus open (vs. closed) and by anterior exit of hemal canal in abdominal vertebra (vs. in complex vertebra); from P. rugosus by coloration of proximal portion of caudal fin similar to rest of caudal fin (vs. clear patch) and from P. quadriradiatus by the total number of pectoral fin-rays six (vs. five). Variable characteristics within Pseudobunocephalus species are summarized and comments on the phylogenetic relationships and the disjunct distribution of the new species are made. [Species zoobank url: urn:lsid:zoobank.org:act:392F95E0-86E1-4386-8779-C4F71098DBCC].

Impact of Tectonic, Glacial and Contour Current Processes on the Late Cenozoic Sedimentary Development of the Southeast Greenland Margin

To understand the geomorphological contrast between the northern and southern parts of the Southeast Greenland margin with its marked differences in sedimentary regime, bathymetric and seismic reflection, data have been compiled and analysed. While previous studies focused on selected parts of this margin, the present study provides an intergraded overview of the entire margin from Cap Farewell to Denmark Strait. The prominent north–south contrast shows a wide northern shelf and a narrow southern shelf. The origin of this width disparity can be traced back to the initial formation stage of the Irminger Sea due to regional differences in uplift versus oceanic subsidence. This regional tectonic discrepancy also created a difference in sediment accommodation space that, in combination with a weak ocean circulation regime, favoured formation of Oligocene–Miocene turbidite fan complexes along the lower southern slope. These fan complexes became the core of sediment drift ridges that strike perpendicular to the slope. Strong bottom currents, which gradually increase in strength towards the south, were mainly prevalent during warmer climate stages. During glacial periods, downslope transport of glacigenic sediments and hyperpycnal meltwater flow further shaped the large drift ridges and formed several relatively narrow, V-shaped turbidite channels extending towards the deep Irminger Sea basin. These V-shaped channels are still active today when cascading dense winter water from the shelf flows downwards along the shelf to the Irminger Sea basin.

Clinical and audiometric outcomes of palisade cartilage myringoplasty under local anesthetic in an office setting.

Assess clinical and functional outcomes of a modified palisade cartilage-perichondrium graft myringoplasty under local in an office setting. Retrospective case series. Tertiary care facility. Patients with a tympanic membrane perforation presenting between March 2013 and October 2017. Inclusion criteria included age ≥ 7 years, entire perforation margin visualized through a transcanal view, and the ability to lie supine for up to 45 min. Exclusion criteria included a conductive hearing loss larger than expected, and presence of active infection. In-office modified myringoplasty technique under local anesthesia without sedation. Complete perforation closure rate and audiometric outcomes. 250 patients underwent the procedure, of whom 13 had bilateral sequential procedures (total 263 ears). Of those, 197 were primary and 66 revision. Average age was 46.3 years. Perforation sizes were categorized as small (32), moderate (109), large (78), and subtotal (44). Complete perforation closure was evident in 219 of the 250 cases (88%). Preoperative mean air pure tone average (PTA) was 56.7 dB and mean bone PTA was 27.5 dB (pre-operative ABG 29.2 dB). AC-PTA significantly improved to 35.0 dB (p < 0.0001), and ABG to 9.6 dB (p < 0.0001). Only subtotal perforations showed a statistically significant negative relationship with outcome (p = 0.04). The modified palisade cartilage-perichondrium graft myringoplasty under local anesthetic is a highly successful procedure well tolerated by adult and pediatric patients with variable perforation sizes. This may have significant potential patient benefits, as well as cost savings to the health care system.

Echeveria xochipalensis (Crassulaceae), a new species from Guerrero, Mexico

Echeveria xochipalensis, from the state of Guerrero, Mexico, is described as a new species to science. It is related to species of ser. Gibbiflorae, particularly to both E. fulgens varieties, from which it is distinguished by its green to lilac-gray spatulate leaves, although they commonly are coppery tinged; the leaves are rigid and with entire margin (not crenate), the margin is yellow-hyaline; bracts oblanceolate with rigid consistency; corolla pink at base and orange at the apex and whitish filaments, ovaries and nectary scales.

Seismicity in Cascadia

We examine spatio-geometric patterns in the density of seismicity within the Cascadia forearc to gain insight into controls on seismogenesis. Tremor epicenters exhibit the most regular distribution defining a 40–80 km wide band extending along almost the entire convergent margin from the southern terminus of Gorda plate subduction in northern California to the central Explorer plate below northern Vancouver Island. Based on prior characterization of constituent low-frequency earthquakes, the up- and down-dip limits of tremor are assumed to represent slab isodepth contours at nominal values of 28 and 45 km, between which fluids at near-lithostatic overpressures are trapped within the subducting slab. Epicenters of earthquakes within the North American crust are anticorrelated with those of tremor, and concentrated in Washington and northern California where they are sandwiched between tremor and the Cascade volcanic arc. Seismicity within the subducting plate possesses the most limited epicentral distribution. Seismicity is confined to shallow depths off Vancouver Island and in northern California, and projects to greater depths beneath Washington and southern British Columbia. Comparison of seismicity patterns with long-wavelength slab geometry and thermo-petrologic constraints suggests that seismicity occurrence in Cascadia is governed by an interplay between slab strain, metamorphic dehydration within the subducting oceanic plate, and a plate boundary seal that controls where fluids enter the overriding plate. The inferred fluid evolution model harbors interesting implications for mantle wedge hydrology, forearc crustal composition and volcanism in Cascadia and other warm subduction zones.

The comparison of the bacterial colonisation of the oral cavity and vaginal ecosystems

Introduction: The right bacterial flora of the vagina is, first of all, lactobacilli. Lactobacilli inhibit the development of pathogens and restore the right bacterial flora of the vagina. Any disorders of the vaginal ecosystem most typically result from epithelium damage, a low concentration of estrogens and atrophy of the right vaginal microflora. In the vagina, both systemic, sexually transmitted infections as well as the infections characteristic of the vagina occur. Among the latter, the following can be distinguished: bacterial vaginosis–the most frequent, candidal vulvovaginitis, trichomoniasis, the infection with aerobic bacteria, and mixed infections (MI). Gestational gingivitis, which is manifested in the erubescence of gingival papillae or the entire gingival margin and increased gingival bleeding during brushing, has every characteristic of an inflammation related to the dental plaque. The pregnant women diagnosed with a periodontium disease are 7.5–20 times more likely to succumb to gestational complications than those with good periodontium.

Mediterranean Lithophyllum stictiforme (Corallinales, Rhodophyta) is a genetically diverse species complex: implications for species circumscription, biogeography and conservation of coralligenous habitats.

Lithophyllum species in the Mediterranean Sea function as algal bioconstructors, contributing to the formation of biogenic habitats such as coralligenous concretions. In such habitats, thalli of Lithophyllum, consisting of crusts or lamellae with entire or lobed margins, have been variously referred to as either one species, L.stictiforme, or two species, L.stictiforme and L.cabiochiae, in the recent literature. We investigated species diversity and phylogenetic relationships in these algae by sequencing three markers (psbA and rbcL genes, cox2,3 spacer), in conjunction with methods for algorithmic delimitation of species (ABGD and GMYC). Mediterranean subtidal Lithophyllum belong to a well-supported lineage, hereby called the L.stictiforme complex, which also includes two species described from the Atlantic, L.lobatum and L.searlesii. Our results indicate that the L.stictiforme complex consists of at least 13 species. Among the Mediterranean species, some are widely distributed and span most of the western and central Mediterranean, whereas others appear to be restricted to specific localities. These patterns are interpreted as possibly resulting from allopatric speciation events that took place during the Messinian Salinity Crisis and subsequent glacial periods. A partial rbcL sequence from the lectotype of L.stictiforme unambiguously indicates that this name applies to the most common subtidal Lithophyllum in the central Mediterranean. We agree with recent treatments that considered L.cabiochiae and L.stictiforme conspecific. The diversity of Lithophyllum in Mediterranean coralligenous habitats has been substantially underestimated, and future work on these and other Mediterranean corallines should use identifications based on DNA sequences.

A new species of Ceradocus Costa, 1853 (Senticaudata: Maeridae) from Ceará State, Northeastern Brazil.

A new species of the genus Ceradocus Costa, 1853 is described from Northeastern Brazil. This species is unique in having: (1) male gnathopod 2 propodus palm ornamented, palmar corner defined by a soft spine; (2) spines along the entire posterior margin of epimeral plate 3, (3) telson with very long apical setae. This new species is the second species of this genus in Brazil. An updated taxonomic key to the Ceradocus of the world is also provided.

A Morphometric Analysis of Serpocaulon gilliesii (Polypodiaceae) Reveals a New Species for Yungas Montane Forest, S. australe

Abstract—A new species, Serpocaulon australe, from the Yungas Montane Forest of Argentina and Bolivia, is described based on a re-interpretation of the name S. gilliesii. The new species is similar to S. lasiopus, but can be distinguished by its rhizome scales with entire margins and acute-acuminate apices, glabrous fronds with few axillary scales in the segments, sori absent in the apex of the segments, and ascending segments. We investigated the taxonomic relationships among these similar species of Serpocaulon using morphoanatomical characters of the sporophyte and ecological preferences. Morphometric analyses, clustering, and principal coordinate analyses (PCoA), were used to scrutinize the forming of hierarchical non-overlapping groups strictly according to degree of morphological similarity. Twenty-four characters were scored for 65 specimens from herbaria (including type material) and for new collections from our fieldwork. We discuss the taxonomic status of S. gilliesii and S. lasiopus and present nomenclature notes for S. gilliesii. Also, some misidentified herbaria collections have led to the discovery of a Serpocaulon species (S. lasiopus), a new record for the Argentinian flora. Finally, a revised key to the pinnatisect species of Serpocaulon in Argentina is provided.