Enteromorpha Compressa Research Articles

DETERMINATION OF BIOLOGICAL ACTIVITIES OF THREE MARINE ALGAE COLLECTED FROM VISAKHAPATNAM COAST

Objective: Determination of biological activities of marine algae collected from the Visakhapatnam coastal region.Methods: Antibacterial activity of algal extracts determined by the well diffusion method, antioxidant activity was determined by reducing power (RP) method and 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging method finally anti-inflammatory activity was determined by human red blood cell stabilization method and egg albumin method.Results: Methanolic extracts of three algae have potential inhibition activity against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. Green alga Enteromorpha compressa has better antioxidant activity compared to the Gracilaria arcuata and Ulva fasciata when tested in RP and DPPH method. U. fasciata found good anti-inflammatory activity among the selected three algae.Conclusion: The three tested algae exhibited significant antibacterial and antioxidant activity compared to anti-inflammatory activity. These bioactive compounds containing macroalgae may find their commercial potential in medicine, food, and cosmetic industry

Comparison of in vitro antioxidant activity of some selected seaweeds from Algerian West Coast

In vitro antioxidant activity of methanolic (ME) and ethanolic (ET) extracts of six species of seaweeds (Cystoseira stricta, Cystoseira compressa, Corallina elongata, Porphyra umbilicalis, Enteromorpha compressa and Ulva lactuca) collected from Algerian West Coast was evaluated, using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay and reducing power assay. They were tested for total phenolic content. The highest phenolic content was observed in C. compressa [10.24 ± 0.09 mg gallic acid equivalent (GAE) g-1 dry weight (DW) in ME extract and 15.70 ± 0.72 mg GAE g-1 DW in ET extract]. In general, the ET seaweed extracts were the most effective fractions. In addition, C. compressa and C. stricta extracts showed the highest antioxidant activity in all assays and better than vitamin E. However, these extracts present lower DPPH radical scavenging activity and reducing power than butylhydroxytoluene (BHT), except at concentration of 1 mg mL-1, in which it was similar. Therefore, C. compressa and C. stricta had a potential to be used as a natural antioxidant agent. Key words: Algerian, antioxidant activity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging, reducing power, seaweed.

Green seaweed Enteromorpha compressa (Chlorophyta, Ulvaceae) derived sulphated polysaccharides inhibit herpes simplex virus

The herpes simplex virus (HSV) diseases represent a relevant medical and social problem due to their communicability and recurrence following latency. The green algae are rich source of polysaccharides referred to as ulvans, reported as being biologically and pharmacologically active. In this work, we analyzed the activity of seven chemically modified polysaccharides from Enteromorpha compressa (Chlorophyta, Ulvaceae), against HSV. Only the derivative named SU1F1 showed satisfactory viral inhibition activity, with a high selectivity index, and, therefore, it was submitted to analysis of the probable mechanism of action and structure. SU1F1 is a sulphated (22% w/w) heteroglycuronan with an apparent molecular mass of 34kDa. The antiviral activity was assayed by plaque reduction assay under the protocols of the time-of-addition (from 3h before infection to 16h after infection), the inhibition of virus adsorption and penetration, and the virucidal effects. SU1F1 showed a high viral activity at the time 0h. We demonstrated that its inhibitory effect was maintained until 4h post-treatment with 100% of viral inhibition at 100μg/ml. No effect was observed in additional protocols (the pre-treatment, the inhibition of adsorption and penetration and virucidal assays). Reverse Transcriptase associated PCR (RT-PCR) results were in accordance with plaque reduction assay and demonstrated the activity of SU1F1 at the initial stages of HSV replication.

Biofabrication and characterization of silver nanoparticles using aqueous extract of seaweed Enteromorpha compressa and its biomedical properties

Green synthesis of nanoparticles using seaweeds are fascinating high research attention nowadays and also gaining center of attention in biomedical applications. In this work, we have synthesized biocompatible and functionalized silver nanoparticles using an aqueous extract of seaweed Enteromorpha compressa as a reducing as well as stabilizing agent and their efficient antimicrobial and anticancer activity are reported here. The UV–vis spectra of AgNPs showed the characteristics SPR absorption band at 421nm. The chemical interaction and crystalline nature of the AgNPs were evaluated by FT-IR and XRD studies. The XRD result of AgNPs shows typical Ag reflection peaks at 38.1°, 44.2°, 64.4° and 77.1° corresponding to (111), (200), (220) and (311) Bragg’s planes. The surface morphology and composition of the samples were observed by HRTEM, EDS and SAED pattern analyses. Spherical shaped Ag nano structures were observed in the size ranges between 4 and 24nm with clear lattice fringes in the HRTEM image. This report reveals that seaweed mediated synthesis of AgNPs and sustained delivery of Ag ions to the bacterial and fungal surface have been reducing their growth rate which was evaluated by well diffusion assay. The synthesized AgNPs showed favorable cytotoxicity against Ehlrich Ascites Carcinoma (EAC) cells with IC50 value was recorded at 95.35μgmL−1. This study showed cost effective silver nanoparticles synthesis with excellent biocompatibility and thus could potentially be utilized in biomedical and pharmaceutical applications.

Macromolecular Antioxidants and Dietary Fiber in Edible Seaweeds.

Seaweeds are rich in different bioactive compounds with potential uses in drugs, cosmetics and the food industry. The objective of this study was to analyze macromolecular antioxidants or nonextractable polyphenols, in several edible seaweed species collected in Chile (Gracilaria chilensis, Callophyllis concepcionensis, Macrocystis pyrifera, Scytosyphon lomentaria, Ulva sp. and Enteromorpha compressa), including their 1st HPLC characterization. Macromolecular antioxidants are commonly ignored in studies of bioactive compounds. They are associated with insoluble dietary fiber and exhibit significant biological activity, with specific features that are different from those of both dietary fiber and extractable polyphenols. We also evaluated extractable polyphenols and dietary fiber, given their relationship with macromolecular antioxidants. Our results show that macromolecular antioxidants are a major polyphenol fraction (averaging 42% to total polyphenol content), with hydroxycinnamic acids, hydroxybenzoic acids and flavonols being the main constituents. This fraction also showed remarkable antioxidant capacity, as determined by 2 complementary assays. The dietary fiber content was over 50% of dry weight, with some samples exhibiting the target proportionality between soluble and insoluble dietary fiber for adequate nutrition. Overall, our data show that seaweed could be an important source of commonly ignored macromolecular antioxidants.

Biodiesel production from different algal oil using immobilized pure lipase and tailor made rPichia pastoris with Cal A and Cal B genes

In this investigation, oil extraction was performed in marine macroalgae Gracilaria edulis, Enteromorpha compressa and Ulva lactuca. The algal biomass was characterized by Scanning Electron Microscopy and Fourier Transform-Infra Red Spectroscopy. Six different pre-treatment methods were carried out to evaluate the best method for maximum oil extraction. Optimization of extraction parameters were performed and high oil yield was obtained at temperature 55°C, time 150min, particle size 0.10mm, solvent-to-solid ratio 6:1 and agitation rate 500rpm. After optimization, 9.5%, 12.18% and 10.50 (g/g) of oil extraction yield was achieved from the respective algal biomass. The rate constant for extraction was obtained as first order kinetics, by differential method. Stable intracellular Cal A and Cal B lipase producing recombinant Pichia pastoris was constructed and used as biocatalyst for biodiesel production. Comparative analysis of lipase activity and biodiesel yield was made with immobilized Candida antarctica lipase.

Proximate Composition of Bio Potential Seaweeds from Mandapam South East Coast of India

Proximate composition of the three green seaweeds viz., Chaetomorpha aerea, Enteromorpha intestinalis, Enteromorpha compressa one brown seaweed Dictyota dichotoma and one red seaweed Gracilaria corticata was investigated by determination of protein, carbohydrates, lipid, moisture and ash content. In the present study, the total protein was recarded upper most in E.intestinalis and bare minimum in E.compressa. The maximum carbohydrates recorded in C. aerea and minimum in G. corticata. The lipid content was acquired upper limit in E. compressa and least in C. aerea. The ash and moisture content in following of 5 different seaweeds are as follows: 7.45mg/g, 3.91mg/g were recorded in the C.aerea, 8.52mg/g, 3.42mg/g in the E.intestinalis, 8.58mg/g, 3.75mg/g in the E.compressa, 9.47mg/g, 4.23mg/g in the D.dichotoma and 6.95mg/g, 3.98mg/g in G.corticata. The proximate composition of 5 different seaweed species exhibited high nutritional value for human consumption.

Proximate Composition of Bio Potential Seaweeds from Mandapam South East Coast of India

Proximate composition of the three green seaweeds viz., Chaetomorpha aerea, Enteromorpha intestinalis, Enteromorpha compressa one brown seaweed Dictyota dichotoma and one red seaweed Gracilaria corticata was investigated by determination of protein, carbohydrates, lipid, moisture and ash content. In the present study, the total protein was recarded upper most in E.intestinalis and bare minimum in E.compressa. The maximum carbohydrates recorded in C. aerea and minimum in G. corticata. The lipid content was acquired upper limit in E. compressa and least in C. aerea. The ash and moisture content in following of 5 different seaweeds are as follows: 7.45mg/g, 3.91mg/g were recorded in the C.aerea, 8.52mg/g, 3.42mg/g in the E.intestinalis, 8.58mg/g, 3.75mg/g in the E.compressa, 9.47mg/g, 4.23mg/g in the D.dichotoma and 6.95mg/g, 3.98mg/g in G.corticata. The proximate composition of 5 different seaweed species exhibited high nutritional value for human consumption.

Enteromorpha compressa macroalgae as biosorbent for heavy metal removal: a preliminary economical evaluation

In this study, the feasibility of using Enteromorpha compressa macroalgae for developing a biosorbent for the use in metal removal from industrial wastewater was discussed in the light of economic models. The net present value and the internal rate of return were used to evaluate the economics of the process. It was concluded that supply to the market as a biosorbent of E. compressa creates an economic benefit to the producing institution.

Bio-Efficacy of Green Seaweeds from South East Coast of Tamil Nadu, India

The present study was aimed to examine the antibacterial potential of Caulerpa corynephora Montagne, Caulerpa scalpelliformis (R. Br.) Weber-van Bosse, Chaetomorpha antennia (Bory de Saint-Vincent) Kutzing, Enteromorpha compressa (L.) Grev., Halimeda macroloba Decsne., Ulva fasciata Delile and Ulva lactuca Linn. from southern east coast of Tamil Nadu, India. For the bioefficacy analysis the pathogens viz., Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Serratia marcescens, Salmonella typhi, Acinetobacter calcoaceticus, Enterobacter cloacae, Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes and Bacillus cereus were procured from Scadder Laboratories. The antibacterial activity was determined by the paper disc diffusion method. 30µg of crude extracts of selected seaweeds were used for the analysis. 30µg and 30µl of the respective solvents were used as positive and negative control. Among the five different extracts examined, antibacterial activities are observed in hexane, petroleum ether, chloroform and ethanolic extracts with varied frequency. Aqueous extracts were failed to show activity against the tested twelve pathogens. In the present study, variability in the antibacterial activity may be related to variability in the bioactive principles present in different seaweeds and organic solvents used for extraction of bioactive compounds. Further purification of the extracts may yield a novel antibacterial drug to treat various diseases typhoid, diarrhoea, pneumonia, meningitis, osteomyelitis, endocarditis, nausea, skin infections etc.

Removal of Cu(2+) by biochars derived from green macroalgae.

The by-product char of the fast pyrolysis of a green macroalga Enteromorpha compressa was used to remove copper from an aqueous solution. The surface area and the amount of cation exchange capacity (CEC) were increased by steam activation, resulting in enhanced adsorption capacity. Although chemical activation using a KOH solution increased the pore volume and surface area dramatically, it decreased the adsorption capacity because of activating in the KOH solution and washing decreased CEC. Ion exchange between the Cu ions and cations (Na(+), K(+), Ca(2+), and Mg(2+)) as well as adsorption onto the functional groups on the char surface appeared to be important mechanisms for the removal of Cu. A pseudo-second-order kinetic model and Langmuir isotherm model could interpret the kinetics and equilibrium of the Cu adsorption on the E. compressa char. The maximum amount of Cu adsorption on the char was 137mgg(-1).

Seaweeds as bioindicators of heavy metals off a hot spot area on the Egyptian Mediterranean Coast during 2008-2010.

Concentrations of Cu, Zn, Cd, Pb, Ni, Co, Fe, Mn, and Hg were measured successively in water, sediments, and six macroalgal species belonging to three algal classes during 3 years (2008-2010) from Abu Qir Bay, Alexandria, Egypt: Chlorophyceae (Enteromorpha compressa, Ulva fasciata), Phaeophyceae (Padina boryana), and Rhodophyceae (Jania rubens, Hypnea musciformis, Pterocladia capillacea). The study aimed to assess the bioaccumulation potential of the seaweeds, as well as to evaluate the extent of heavy metal contamination in the selected study site. Metals were analyzed using atomic absorption spectrophotometry coupled with MH-10 hydride system. The obtained data showed that the highest mean concentrations of Cu, Zn, Fe, and Mn were recorded in E. compressa; Cd, Ni, and Hg exhibited their highest mean concentrations in P. boryana, while Pb and Co were found in J. rubens. Abundance of the heavy metals in the algal species was as follow: Fe > Mn > Zn > Pb > Ni > Co > Cu > Cd > Hg. E. compressa showed the maximum metal pollution index (MPI) which was 11.55. Bioconcentration factor (BCF) for the metals in algae was relatively high with a maximum value for Mn. The Tomlinson pollution load index (PLI) values for the recorded algal species were low, which ranged between 1.00 in P. boryana and 2.72 in E. compressa. Enrichment factors for sediments were low fluctuating between 0.43 for Hg to 2.33 for Mn. Accordingly, the green alga E. compressa, brown alga P. boryana, and red alga J. rubens can be nominated as bioindicators. Based on MPI and PLI indices, Abu Qir Bay in the present study is considered as low-contaminated area.

Ultrasound-enhanced rapid in situ transesterification of marine macroalgae Enteromorpha compressa for biodiesel production

In situ transesterification of Enteromorpha compressa algal biomass was carried out for the production of biodiesel. The maximum methyl esters (ME) yield of 98.89% was obtained using ultrasonic irradiation. Tetra hydro furan (THF) and acid catalyst (H2SO4) was found to be an appropriate co-solvent and catalyst for high free fatty acids (FFA) content E. compressa biomass to increase the efficiency of the reactive in situ process. The optimization study was conducted to obtain the maximum yield and it was determined as 30vol% of THF as a co-solvent, 10wt% of H2SO4, 5.5:1 ratio of methanol to algal biomass and 600rpm of mixing intensity at 65°C for 90min of ultrasonic irradiation time. The produced biodiesel was characterized by (1)H nuclear magnetic resonance spectroscopy ((1)H NMR) analysis. Kinetic studies revealed that the reaction followed the first-order reaction mechanism. Rapid in situ transesterification was found to be suitable technique to produce biodiesel from marine macroalgae feedstock.

Distribution of heavy metals in seaweeds collected along Marsa-Matrouh beaches, Egyptian Mediterranean Sea

The distribution of Cd, Cu, Fe, Ni, Pb, and Zn concentration in two seagrass species in addition to six species of marine macro algae: Cystoseira sp., Gelidium crinale, Laurencia obtusa, Gracilaria verrucosa, Jania rubens, and Enteromorpha compressa collected along Marsa-Matrouh coastal waters (Egypt) were determined by Flame Atomic Absorption Spectrometry. The recovery study was carried out using a certified reference material NMIJ CRM 7405-a. The obtained heavy metal contents indicated that different species demonstrated various degrees of metal accumulation. In general, El-Boussit station recorded the highest concentrations among the all studied stations. There was a positive correlation between Fe concentrations in the seaweeds and those of Cu and Pb indicated the same source of these metals and/or synergistic interaction between these metals. In general, as regards the influence of the collection sites on the whole metal accumulation, El-Boussit is considered to be the most polluted. There was a positive correlation between Fe concentrations in the seaweeds and those of the Cu and Pb, in addition to negative correlations between Cu and Ni, Pb and Zn. On the basis of the levels of trace elements observed in Posidonia oceanica and Cymodocea nodosa besides their wide distribution in Marsa-Matrouh beaches; these two species are considered to be good bio-indicators for metals in the area of investigation.

Production of algal biodiesel from marine macroalgae Enteromorpha compressa by two step process: Optimization and kinetic study

In this investigation, Enteromorpha compressa algal oil with high free fatty acids (FFA) used as a feedstock for biodiesel production. Two step process was developed and kinetic study executed to obtain reaction rate constant for the transesterification reaction. The acid esterification was carried out to reduce FFA from 6.3% to 0.34% with optimized parameters of 1.5% H2SO4, 12:1 methanol–oil ratio, 400rpm at 60°C and 90min of reaction time. The maximum biodiesel yield 90.6% was achieved from base transesterification through optimum conditions of 1% NaOH, 9:1 methanol–oil ratio, 600rpm and 60°C temperature for 70min. The algal biodiesel was characterized by GC–MS, HPLC and NIR. This transesterification follows first order reaction kinetics and the activation energy was determined as 73,154.89J/mol. The biodiesel properties were analyzed and found to be within the limits of American standards. Hence, E. compressa serves as a valuable renewable raw-material for biodiesel production.

사람피부섬유아세포 및 섬유아육종세포로부터 유래된 기질금속단백질효소에 대한 해조류의 효능

In recent years novel potential pharmocological candidates have been looked for in animal, seaweed, sponge, fungi and marine bacteria resources. In this study, matrix metalloproteinases (MMPs) that play an important role in metastasis, arthritis, chronic inflammation and wrinkle formation were used as target enzymes to screen therapeutic agents. The inhibitory effects of several marine algae including green algae (5 species), red algae (18 species) and brown algae (4 species) methanolic extracts on MMPs were investigated in human dermal fibroblasts and human fibrosarcoma cell line (HT1080 cells) using gelatin zymography. In human dermal fibroblasts, the inhibition of MMP-2 was observed in Laurencia okamurae, Polysiphonia japonica, Grateloupia lanceolate and Sinkoraena lancifolia of red algae. In contrast, MMP-2 activation was enhanced in Enteromorpha compressa and E. linza of green algae, and Peltaronia bighamiae and Sargassum thunbergii of brown algae. In human fibrosarcoma cells, MMP-9 activation was decreased in the presence of S. thunbergii of brown algae, Polysiphonia japonica in red algae and E. compressa and E. linza of green algae. The interesting finding is that E. compressa and E. linza of green algae, and S. thunbergii of brown algae exhibited a positive effect on MMP-2 in normal cells, but a negative effect on MMP-9 in cancer cell lines. These results suggest that E. compressa and E. linza of green algae, and S. thunbergii of brown algae contain potential therapeutic ingredients for cancer treatment.

Application of centrifugal precipitation chromatography and high‐speed counter‐current chromatography equipped with a spiral tubing support rotor for the isolation and partial characterization of carotenoid cleavage‐like enzymes in Enteromorpha compressa (L.) Nees

Centrifugal precipitation chromatography and a high-speed counter-current chromatography system equipped with a spiral tubing support rotor (spHSCCC) were successfully applied for the identification and isolation of carotenoid cleavage-like enzymes from Enteromorpha compressa (L.) Nees. This is the first study separating active enzymes from a complex natural matrix by spHSCCC. The target enzymes were identified after fractionation of the proteins in an acetone Tris-buffer gradient by centrifugal precipitation chromatography. Also, an aqueous two-phase solvent system consisting of PEG 1000 and mono- and dibasic potassium phosphate was used for the isolation of the enzymes by spHSCCC. The purified fractions contained two proteins of 65 and 72 kDa, respectively. The enzymes could cleave β-carotene and β-apo-8'-carotenal to produce β-ionone.

Production of agarase from a novel Micrococcus sp. GNUM-08124 strain isolated from the East Sea of Korea

The agar degrading bacterial strain GNUM-08124 was isolated from Enteromorpha compressa collected in the East Sea of Korea by using a selective artificial sea water (ASW) agar plate containing agar as the sole carbon source. GNUM-08124 grows to produce a circular, smooth, yellow-colored, and raised colony. Its ability to hydrolyze agar was confirmed by staining the ASW agar plate with Lugol’s solution. In liquid culture, the cell density (A600) increased exponentially and reached a maximum level on the third day of cultivation. The specific agarase activity also increased in proportion to the cell density and reached maximum agarolytic activity on the third day. The 16S rRNA sequence of GNUM-08124 showed a close relationship to Micrococcus luteus (99.65%) and Micrococcus endophyticus (99.15%), which led us to assign it to the genus Micrococcus. Physiological studies indicated that optimal growth conditions were between 30 and 40°C, pH 4 and 7, using media containing between 5 and 10% NaCl (w/v), respectively. The GNUM-08124 strain was a grampositive, urease-positive, and catalase-positive bacterium. It could not hydrolyze gelatin, cellulose, xylan, or starch, but fermented a broader range of substrates, including Dglucose, D-galactose, D-fructose, D-lactose, D-trehalose, D-mannitol, D-melibiose, D-raffinose, D-xylose, methyl-α-D-glucopyranoside, N-acetyl-glucosamine, and xylitol, than those fermented by M. luteus or M. endophyticus, suggesting GNUM-08124 is a novel agar hydrolyzing microorganism belonging to Genus Micrococcus. Micrococcus sp. GNUM-08124 showed the highest agarase activity when it was cultured in ASW-YP medium supplemented with 0.4% glucose, but demonstrated lower activity in rich media (LB or TSB), in spite of superior cell growth, implying that agarase production is tightly regulated in an agar-dependent manner and repressed in rich conditions.

Enteromorpha compressa Exhibits Potent Antioxidant Activity

The green macroalgae, Enteromorpha compressa (Linnaeus) Nees, Ulva lactuca, and E. linza, were seasonally collected from Abu Qir bay at Alexandria (Mediterranean Sea) This work aimed to investigate the seasonal environmental conditions, controlling the green algal growth, predominance, or disappearance and determining antioxidant activity. The freshly collected selected alga (E. compressa) was subjected to pigment analysis (chlorophyll and carotenoids) essential oil and antioxidant enzyme determination (ascorbate oxidase and catalase). The air-dried ground alga was extracted with ethanol (crude extract) then sequentially fractionated by organic solvents of increasing polarity (petroleum ether, chloroform, ethyl acetate, and water). Antioxidant activity of all extracts was assayed using different methods (total antioxidant, DPPH [2, 2 diphenyl-1-picrylhydrazyl], ABTS [2, 2 azino-bis ethylbenzthiazoline-6-sulfonic acid], and reducing power, and β-carotene linoleic acid bleaching methods). The results indicated that the antioxidant activity was concentration and time dependent. Ethyl acetate fraction demonstrated higher antioxidant activity against DPPH method (82.80%) compared to the synthetic standard butylated hydroxyl toluene (BHT, 88.5%). However, the crude ethanolic extract, pet ether, chloroform fractions recorded lower to moderate antioxidant activities (49.0, 66.0, and 78.0%, resp.). Using chromatographic and spectroscopic analyses, an active compound was separated and identified from the promising ethyl acetate fraction.

Biosorption Kinetics and Isotherm Studies of Cd(II) by Dried Enteromorpha compressa Macroalgae Cells from Aqueous Solutions

AbstractFor the first time ever, Enteromorpha compressa macroalgae (ECM), which is commonly found in Turkey, has been used as biosorbent by us. This study aims to investigate the biosorption of Cd2+ from aqueous solutions in a batch system by using an alga of ECM in different concentrations, pH levels, agitation rates (90–150 rpm), and contact periods. The maximum biosorption capacity of the ECM was found to be 9.50 mg/g at pH 6, Cd2+ initial concentration of 10 mg/L and agitation rate 150 rpm. Cadmium removal efficiency was about 95%. The experimental isotherm data were analyzed using the Langmuir and Freundlich equations. Isotherm parameters for both equations were determined and discussed. The stated biosorption mechanism is explained by the Freundlich isotherm (r2 = 0.998) theory. Two simplified kinetic models including a pseudo‐first‐ and second‐order equation were selected to follow the biosorption process. Kinetic parameters; rate constants, equilibrium adsorption capacities and related correlation coefficients, for each kinetic model were calculated and discussed. It was shown that the biosorption of cadmium onto ECM could be described by the pseudo‐second‐order equation (r2 > 0.99).