HighlightsBench-scale pig buildings are suitable for studying airborne contaminants of commercial pig buildings.Multi-replicate designed experiments are possible using bench-scale pig buildings (experimental chambers).Strategies to reduce airborne contaminants may be developed in a timely and cost-effective way using the chambers.Abstract. Pig buildings produce and emit large amounts of airborne contaminants into the environment. Odors, gases, dust, and bioaerosols are responsible for the emergence of infectious, toxic, and inflammatory diseases diagnosed in animals, workers, and surrounding communities. There is therefore a need for better characterization and mitigation of airborne contaminants in commercial pig buildings. Bench-scale pig buildings could be used for the development and evaluation of reduction strategies for airborne contaminants in a timely and cost-effective manner before scaling them up for commercial use. Six bench-scale pig buildings were designed for growing animals (commercial diet and fully slatted concrete floor) and for producing odors, gases, dust, and bioaerosols from various sources (e.g., feed, manure) and animal activities. The validation of the six identical, environmentally controlled, independently ventilated and sealed bench-scale pig buildings (7.1 m3, 42 air changes per hour) is reported here. The validation implied inter-building and longitudinal (between week) comparisons of concentrations of airborne contaminants. Environmental conditions, methane (CH4), carbon dioxide (CO2), nitrous oxide (N2O), ammonia (NH3), and airborne microorganisms (total bacteria and archaea, Clostridium perfringens, Enterococcus spp., and Escherichia coli) were monitored for seven weeks in the six bench-scale pig buildings. The experiment was repeated three times. From week to week, data analysis showed that environmental conditions, gases, odors, and airborne microbial concentrations were non-statistically different between buildings. By the end of the seven-week experiment, concentrations of N2O, NH3, Enterococcus, E. coli, and archaea were higher than during week 1 in all buildings (p < 0.05). Airborne gases, odors, and microbial concentrations were equivalent to those found during the Canadian summer in commercial finishing pig buildings. In conclusion, the designed bench-scale pig buildings may serve for multi-replicate experiments and for studying airborne contaminants found in commercial pig buildings. Keywords: Airborne contaminant, Bioaerosols, Dust, Gas, Odor, Pig building.