Enteric Methane Mitigation Research Articles

Role of Secondary Plant Metabolites on Enteric Methane Mitigation in Ruminants.

The rumen microbiome plays a fundamental role in all ruminant species, it is involved in health, nutrient utilization, detoxification, and methane emissions. Methane is a greenhouse gas which is eructated in large volumes by ruminants grazing extensive grasslands in the tropical regions of the world. Enteric methane is the largest contributor to the emissions of greenhouse gases originating from animal agriculture. A large variety of plants containing secondary metabolites [essential oils (terpenoids), tannins, saponins, and flavonoids] have been evaluated as cattle feedstuffs and changes in volatile fatty acid proportions and methane synthesis in the rumen have been assessed. Alterations to the rumen microbiome may lead to changes in diversity, composition, and structure of the methanogen community. Legumes containing condensed tannins such as Leucaena leucocephala have shown a good methane mitigating effect when fed at levels of up to 30–35% of ration dry matter in cattle as a result of the effect of condensed tannins on rumen bacteria and methanogens. It has been shown that saponins disrupt the membrane of rumen protozoa, thus decreasing the numbers of both protozoa and methanogenic archaea. Trials carried out with cattle housed in respiration chambers have demonstrated the enteric methane mitigation effect in cattle and sheep of tropical legumes such as Enterolobium cyclocarpum and Samanea saman which contain saponins. Essential oils are volatile constituents of terpenoid or non-terpenoid origin which impair energy metabolism of archaea and have shown reductions of up to 26% in enteric methane emissions in ruminants. There is emerging evidence showing the potential of flavonoids as methane mitigating compounds, but more work is required in vivo to confirm preliminary findings. From the information hereby presented, it is clear that plant secondary metabolites can be a rational approach to modulate the rumen microbiome and modify its function, some species of rumen microbes improve protein and fiber degradation and reduce feed energy loss as methane in ruminants fed tropical plant species.

Invited Review: Methane sources, quantification, and mitigation in grazing beef systems

ABSTRACT Purpose The purpose of the review was to examine enteric methane emissions, quantification methods, and mitigation strategies in grazing beef systems. Sources Peer-reviewed literature and conference abstracts were the main sources of information for this review. Synthesis Methane emissions (CH4) can be reduced by improving forage quality by including more cool-season forages and legumes and rotationally grazing animals. Including forages with beneficial secondary compounds such as condensed tannins and saponins also has CH4-mitigation potential. Providing nutritional supplements that improve the nutritional status of the animal and the efficiency of feed energy use has the potential to reduce CH4 emissions from grazing cattle. Genetic selection has shown some viability in reducing herd emissions, but heritability estimates are low for CH4 yield. More research is needed to understand the potential. Soil methanotrophy may partially offset CH4 emissions when animals are stocked moderately but soil CH4 uptake rates are relatively low in most grazing ecosystems. A new metric to quantify the global warming potential of CH4, GWP*, may allow future models to more appropriately consider the behavior and effects of CH4 in the atmosphere. Conclusions and Applications Methane mitigation strategies in grazing environments are limited, but producer decisions that improve the nutritional status of animals, the quality of the forage base, and supplementation of known CH4-mitigation compounds can reduce CH4 production. Now that less expensive, easier to use quantification tools exist, researchers need to conduct more long-term monitoring experiments and focus on reducing CH4 production of grazing animals where potential for reduction is largest.

Ambition Meets Reality: Achieving GHG Emission Reduction Targets in the Livestock Sector of Latin America

Livestock production is a pivotal source of income and agricultural greenhouse gas (GHG) emissions in Colombia, Brazil, Argentina, Costa Rica, Uruguay, Mexico and Peru. Several management and technological options, with enteric methane mitigation potential, have been evaluated and their scaling is anticipated to contribute towards achieving GHG emission reduction targets. Yet, widespread adoption of promising mitigation options remains limited, raising questions as to whether envisaged emission reduction targets are achievable. Using locally generated data, we explore the mitigation potentials of technologies and management practices currently proposed to mitigate enteric methane emissions, for cattle production systems in the higher emitting countries of Latin America. We then discuss barriers for adopting innovations that significantly reduce cattle-based enteric methane emissions and the major shifts in policy and practice that are needed to raise national ambitions in the high emitting countries. Using the latest science and current thinking, we provide our perspective on an inclusive approach and re-imagine how the academic, research, business and public policy sectors can support and incentivize the changes needed to raise the level of ambition and achieve sustainable development goals considering actions all the way from the farm to the national scale.

The Role of Chitosan as a Possible Agent for Enteric Methane Mitigation in Ruminants

Simple SummaryRuminant husbandry is one the largest contributors to greenhouse gas emissions from the agriculture sector, particularly of methane gas, which is a byproduct of the anaerobic fermentation of structural and non-structural carbohydrates in the rumen. Increasing the efficiency of production systems and decreasing its environmental burden is a global commitment, thus methane mitigation is a strategy in which to reach these goals by rechanneling metabolic hydrogen (H2) into volatile fatty acids (VFA) to reduce the loss of energy as methane in the rumen, which ranges from 2% (grain rations) to 12% (poor-quality forage rations) of gross energy intake. A strategy to achieve that goal may be through the manipulation of rumen fermentation with natural compounds such as chitosan. In this review, we describe the effects of chitosan on feed intake and rumen fermentation, and present some results on methanogenesis. The main compounds with antimethanogenic properties are the secondary metabolites, which are generally classified into five main groups: saponins, tannins, essential oils, organosulfurized compounds, and flavonoids. Novel compounds of interest include chitosan obtained by the deacetylation of chitin, with beneficial properties such as biocompatibility, biodegradability, non-toxicity, and chelation of metal ions. This compound has shown its potential to modify the rumen microbiome, improve nitrogen (N) metabolism, and mitigate enteric methane (CH4) under some circumstances. Further evaluations in vivo are necessary at different doses in ruminant species as well as the economic evaluation of its incorporation in practical rations.Livestock production is a main source of anthropogenic greenhouse gases (GHG). The main gases are CH4 with a global warming potential (GWP) 25 times and nitrous oxide (N2O) with a GWP 298 times, that of carbon dioxide (CO2) arising from enteric fermentation or from manure management, respectively. In fact, CH4 is the second most important GHG emitted globally. This current scenario has increased the concerns about global warming and encouraged the development of intensive research on different natural compounds to be used as feed additives in ruminant rations and modify the rumen ecosystem, fermentation pattern, and mitigate enteric CH4. The compounds most studied are the secondary metabolites of plants, which include a vast array of chemical substances like polyphenols and saponins that are present in plant tissues of different species, but the results are not consistent, and the extraction cost has constrained their utilization in practical animal feeding. Other new compounds of interest include polysaccharide biopolymers such as chitosan, mainly obtained as a marine co-product. As with other compounds, the effect of chitosan on the rumen microbial population depends on the source, purity, dose, process of extraction, and storage. In addition, it is important to identify compounds without adverse effects on rumen fermentation. The present review is aimed at providing information about chitosan for dietary manipulation to be considered for future studies to mitigate enteric methane and reduce the environmental impact of GHGs arising from livestock production systems. Chitosan is a promising agent with methane mitigating effects, but further research is required with in vivo models to establish effective daily doses without any detrimental effect to the animal and consider its addition in practical rations as well as the economic cost of methane mitigation.

Dietary nitrate metabolism and enteric methane mitigation in sheep consuming a protein-deficient diet

It was hypothesised that the inclusion of nitrate (NO3–) or cysteamine hydrochloride (CSH) in a protein deficient diet (4.8% crude protein; CP) would improve the productivity of sheep while reducing enteric methane (CH4) emissions. A complete randomised designed experiment was conducted with yearling Merino sheep (n = 24) consuming a protein-deficient wheaten chaff control diet (CON) alone or supplemented with 1.8% nitrate (NO3–; DM basis), 0.098% urea (Ur, DM basis) or 80 mg cysteamine hydrochloride/kg liveweight (CSH). Feed intake, CH4 emissions, volatile fatty acids (VFA), digesta kinetics and NO3–, nitrite (NO2–) and urea concentrations in plasma, saliva and urine samples were measured. There was no dietary effect on animal performance or digesta kinetics (P > 0.05), but adding NO3– to the CON diet reduced methane yield (MY) by 26% (P = 0.01). Nitrate supplementation increased blood MetHb, plasma NO3– and NO2– concentrations (P < 0.05), but there was no indication of NO2– toxicity. Overall, salivary NO3– concentration was greater than plasma NO3– (P < 0.05), indicating that NO3– was concentrated into saliva. Our results confirm the role of NO3– as an effective additive to reduce CH4 emissions, even in a highly protein-deficient diet and as a source of additional nitrogen (N) for microbial protein synthesis via N-recycling into saliva and the gut. The role of CSH as an additive in low quality diets for improving animal performance and reducing CH4 emissions is still unclear.

Associative effects of plant secondary metabolites in modulating in vitro methanogenesis, volatile fatty acids production and fermentation of feed in buffalo (Bubalus bubalis)

The present study was conducted to examine the plant bioactive compounds individually and in association for modulation of rumen fermentation in buffalo (Bubalus bubalis) with the aim to develop phytogenic feed additive for enteric methane mitigation from ruminants. The extracts of Sapindus mukorossi (SMF) fruits (aqueous and ethanolic) as a source of saponins, Ficus bengalensis (FBL) leaves (aqueous and acetonic) as a source of tannins and Eucalyptus globulus oils (ECO) as a source of essential oils were prepared and evaluated individually and in association for their effect on feed fermentation and methanogenesis by four separate in vitro experiments. Each experiment was conducted as a completely randomized design using a control and various treatment groups with different concentrations of plant bioactive compounds and their blends. Rumen fluid inoculum was collected from four rumen fistulated Murrah (B. bubalis) buffalo steers. The in vitro incubations were carried out for a period of 24 h with five replicates for each treatment. For SMF and FBL extracts, a concentration of 0, 0.5, 1.0, 2.0 and 4.0 mL were tested, whereas, ECO was tested at the levels of 0, 20, 40, 80 and 120 µL per 40 mL buffered rumen fluid with 0.4 g of oats hay as substrate. Both aqueous or ethanol extracts of SMF, acetonic extract of FBL, and ECO showed linear decrease (p < 0.001) in methane production with increasing concentrations of plant compound. Out of four blends tested, blend-1 (ECO, 125 µL; SMF aqueous extract and FBL acetonic extract, 6.25 mL each per L rumen fluid) showed reduced methane production without affecting negatively to rumen fermentation at much lower individual doses, representing positive associative effect. It is implied that the extracts from S. mukorossi fruits, F. bengalensis leaves and E. globulus essential oils and their blends have the potential to act as anti methanogenic agents. A positive associative effect in reducing enteric methanogenesis in their blends signifies their application as the phytogenic feed additive in ruminants.

Changes in the Rumen Microbiota of Cows in Response to Dietary Supplementation with Nitrate, Linseed, and Saponin Alone or in Combination.

Dietary supplementation with linseed, saponins, and nitrate is a promising methane mitigation strategy in ruminant production. Here, we aimed to assess the effects of these additives on the rumen microbiota in order to understand underlying microbial mechanisms of methane abatement. Two 2-by-2 factorial design studies were conducted simultaneously, which also allowed us to make a broad-based assessment of microbial responses. Eight nonlactating cows were fed diets supplemented with linseed or saponin in order to decrease hydrogen production and nitrate to affect hydrogen consumption; also, combinations of linseed plus nitrate or saponin plus nitrate were used to explore the interaction between dietary treatments. Previous work assessed effects on methane and fermentation patterns. Rumen microbes were studied by sequencing 18S and 16S rRNA genes and ITS1 amplicons. Methanogen activity was monitored by following changes in mcrA transcript abundance. Nitrate fed alone or in combination in both studies dramatically affected the composition and structure of rumen microbiota, although impacts were more evident in one of the studies. Linseed moderately modified only bacterial community structure. Indicator operational taxonomic unit (OTU) analysis revealed that both linseed and nitrate reduced the relative abundance of hydrogen-producing Ruminococcaceae Linseed increased the proportion of bacteria known to reduce succinate to propionate, whereas nitrate supplementation increased nitrate-reducing bacteria and decreased the metabolic activity of rumen methanogens. Saponins had no effect on the microbiota. Inconsistency found between the two studies with nitrate supplementation could be explained by changes in microbial ecosystem functioning rather than changes in microbial community structure.IMPORTANCE This study aimed at identifying the microbial mechanisms of enteric methane mitigation when linseed, nitrate, and saponins were fed to nonlactating cows alone or in a combination. Hydrogen is a limiting factor in rumen methanogenesis. We hypothesized that linseed and saponins would affect hydrogen producers and nitrate would affect hydrogen consumption, leading to reduced methane production in the rumen. Contrary to what was predicted, both linseed and nitrate had a deleterious effect on hydrogen producers; linseed also redirected hydrogen consumption toward propionate production, whereas nitrate stimulated the growth of nitrate-reducing and, hence, hydrogen-consuming bacterial taxa. This novel knowledge of microbial mechanisms involved in rumen methanogenesis provides insights for the development and optimization of methane mitigation strategies.

Effects of naturally-produced lovastatin on feed digestibility, rumen fermentation, microbiota and methane emissions in goats over a 12-week treatment period.

Twenty male Saanen goats were randomly assigned to four levels of lovastatin supplementation and used to determine the optimal dosage and sustainability of naturally produced lovastatin from fermentation of palm kernel cake (PKC) with Aspergillus terreus on enteric methane (CH4) mitigation. The effects on ruminal microbiota, rumen fermentation, feed digestibility and health of animal were determined over three measuring periods (4-, 8- and 12-weeks) and the accumulation of lovastatin in tissues was determined at the end of the experiment. The diets contained 50% rice straw, 22.8% concentrates and 27.2% of various proportions of untreated or treated PKC to achieve the target daily intake level of 0 (Control), 2, 4 or 6 mg lovastatin/kg body weight (BW). Enteric CH4 emissions per dry matter intake (DMI), decreased significantly (P<0.05) and equivalent to 11% and 20.4%, respectively, for the 2 and 4 mg/kg BW groups as compared to the Control. No further decrease in CH4 emission thereafter with higher lovastatin supplementation. Lovastatin had no effect on feed digestibility and minor effect on rumen microbiota, and specifically did not reduce the populations of total methanogens and Methanobacteriales (responsible for CH4 production). Similarly, lovastatin had little effect on rumen fermentation characteristics except that the proportion of propionate increased, which led to a decreasing trend (P<0.08) in acetic: propionate ratio with increasing dosage of lovastatin. This suggests a shift in rumen fermentation pathway to favor propionate production which serves as H+ sink, partly explaining the observed CH4 reduction. No adverse physiological effects were noted in the animals except that treated PKC (containing lovastatin) was less palatable at the highest inclusion level. Lovastatin residues were detected in tissues of goats fed 6 mg lovastatin/kg BW at between 0.01 to 0.03 μg/g, which are very low.

Short communication: Development of an equation for estimating methane emissions of dairy cows from milk Fourier transform mid-infrared spectra by using reference data obtained exclusively from respiration chambers

Evaluation and mitigation of enteric methane (CH4) emissions from ruminant livestock, in particular from dairy cows, have acquired global importance for sustainable, climate-smart cattle production. Based on CH4 reference measurements obtained with the SF6 tracer technique to determine ruminal CH4 production, a current equation permits evaluation of individual daily CH4 emissions of dairy cows based on milk Fourier transform mid-infrared (FT-MIR) spectra. However, the respiration chamber (RC) technique is considered to be more accurate than SF6 to measure CH4 production from cattle. This study aimed to develop an equation that allows estimating CH4 emissions of lactating cows recorded in an RC from corresponding milk FT-MIR spectra and to challenge its robustness and relevance through validation processes and its application on a milk spectral database. This would permit confirming the conclusions drawn with the existing equation based on SF6 reference measurements regarding the potential to estimate daily CH4 emissions of dairy cows from milk FT-MIR spectra. A total of 584 RC reference CH4 measurements (mean ± standard deviation of 400 ± 72 g of CH4/d) and corresponding standardized milk mid-infrared spectra were obtained from 148 individual lactating cows between 7 and 321 d in milk in 5 European countries (Germany, Switzerland, Denmark, France, and Northern Ireland). The developed equation based on RC measurements showed calibration and cross-validation coefficients of determination of 0.65 and 0.57, respectively, which is lower than those obtained earlier by the equation based on 532 SF6 measurements (0.74 and 0.70, respectively). This means that the RC-based model is unable to explain the variability observed in the corresponding reference data as well as the SF6-based model. The standard errors of calibration and cross-validation were lower for the RC model (43 and 47 g/d vs. 66 and 70 g/d for the SF6 version, respectively), indicating that the model based on RC data was closer to actual values. The root mean squared error (RMSE) of calibration of 42 g/d represents only 10% of the overall daily CH4 production, which is 23 g/d lower than the RMSE for the SF6-based equation. During the external validation step an RMSE of 62 g/d was observed. When the RC equation was applied to a standardized spectral database of milk recordings collected in the Walloon region of Belgium between January 2012 and December 2017 (1,515,137 spectra from 132,658 lactating cows in 1,176 different herds), an average ± standard deviation of 446 ± 51 g of CH4/d was estimated, which is consistent with the range of the values measured using both RC and SF6 techniques. This study confirmed that milk FT-MIR spectra could be used as a potential proxy to estimate daily CH4 emissions from dairy cows provided that the variability to predict is covered by the model.

Effect of concentrate feeding level on methane emissions, production performance and rumen fermentation of Jersey cows grazing ryegrass pasture during spring

Dietary supplementation has been well documented as an effective enteric methane (CH4) mitigation strategy. However, limited studies have demonstrated the effect of concentrate level on enteric CH4 emissions from grazing dairy cows, and to our knowledge none of these studies included a pasture-only diet or reported on rumen fermentation measures. Sixty multiparous (4.0 ± 1.51 SD) Jersey cows, of which six were rumen-cannulated, were used in a randomised complete block design, and the cannulated cows were used in a separate replicated 3 × 3 Latin square design, to investigate the effect of concentrate supplementation (0, 4, and 8 kg/cow per day; as fed) on enteric CH4 emissions, milk production, dry matter intake (DMI), and rumen fermentation of dairy cows grazing perennial ryegrass pasture during spring, following a 14-d adaptation period. The sulphur hexafluoride tracer gas technique was used to measure enteric CH4 emissions from 10 cows of each treatment group over a single 9-d measurement period. Parallel with the CH4 measurement period, pasture DMI was determined using TiO2 and indigestible neutral detergent fibre as external and internal markers, respectively, while milk yield, milk composition, cow condition, and pasture pre- and post-grazing measurements were also recorded. Total DMI (13.4 to 18.0 kg/d), milk yield (12.9 to 19.2 kg/d), energy corrected milk (14.6 to 20.7 kg/d), milk lactose content (46.2 to 48.1 g/kg) and gross energy intake (239 to 316 MJ/d) increased, while milk fat content (50.0 to 44.2 g/kg) decreased with increasing concentrate feeding level. Volatile fatty acid concentrations and ruminal pH were mostly unaffected by treatment, while dry matter disappearance decreased and NH3-N concentration increased with increasing concentrate feeding level. Methane production (258 to 302 g/d) and CH4 yield (20.6 to 16.9 g/kg of DMI) were similar for all cows, while pasture DMI (13.4 to 10.8 kg/d) and CH4 intensity (20.4 to 15.9 g of CH4/kg of milk yield) decreased linearly with increasing concentrate feeding level. Results indicate that concentrate supplementation on high quality pasture-only diets have the potential to effectively reduce CH4 emissions per unit of milk yield from grazing cows during spring.

Potential of Samanea saman pod meal for enteric methane mitigation in crossbred heifers fed low-quality tropical grass

Pods of Samanea saman have been reported to be effective in mitigating methane (CH4) emissions in ruminants. The aim of this study was to evaluate the effect of inclusion of S. saman pod meal in the ration on feed intake, apparent digestibility, rumen fermentation, rumen degradation, protozoa population, and enteric CH4 emissions in crossbred heifers fed low-quality tropical grass. Four crossbred heifers (Bos taurus × B. indicus) with an average live weight (LW) of 261.5 ± 1.29 kg were used in a 4 × 4 Latin square design with four periods of 23 days (17 days for adaptation and 6 days for measurements of response variables). Levels of inclusion of S. saman ground pods in the ration were 0, 10, 20 and 30% of dry matter (DM). Enteric CH4 emissions were measured using open-circuit respiration chambers. Additionally, rumen degradation of the different components of S. saman pods was assessed. Incorporation of S. saman levels had no effect on dry matter intake (DMI) (P > .05), apparent digestibility of dry matter (DM) and organic matter (OM) (P > .05) nor protozoal count (P > .05). It was found that molar proportion of propionic acid in rumen liquid was increased (P < .05), while that of acetic acid and acetic:propionic ratio was linearly decreased (P < .01) when heifers were supplemented with S. saman. Additionally, emissions of enteric CH4 showed a linear reduction as the inclusion of S. saman in the ration was increased (P = .007). A reduction of 50.9% in L CH4/day and 56.9% in L CH4/kg DM intake was observed at the highest inclusion level of S. saman compared to the control diet (P < .05). Potential degradation of DM of pods of S. saman was 79.6%. It is concluded that, the inclusion of S. saman pods in the ration has the capacity to reduce energy losses in the form of methane emissions in heifers fed low-quality tropical grass.

Mode of action of Saccharomyces cerevisiae in enteric methane mitigation in pigs

The objectives of this study were to determine the effect and mode of action of Saccharomyces cerevisiae (YST2) on enteric methane (CH4) mitigation in pigs. A total of 12 Duroc×Landrace×Yorkshire male finisher pigs (60±1 kg), housed individually in open-circuit respiration chambers, were randomly assigned to two dietary groups: a basal diet (control); and a basal diet supplemented with 3 g/YST2 (1.8×1010 live cells/g) per kg diet. At the end of 32-day experiment, pigs were sacrificed and redox potential (Eh), pH, volatile fatty acid concentration, densities of methanogens and acetogens, and expression of methyl coenzyme-M reductase subunit A gene were determined in digesta contents from the cecum, colon and rectum. Results showed that S. cerevisiae YST2 decreased (P<0.05) the average daily enteric CH4 production by 25.3%, lowered the pH value from 6.99 to 6.69 in the rectum, and increased the Eh value in cecum and colon by up to −55 mV (P<0.05). Fermentation patterns were also altered by supplementation of YST2 as reflected by the lower acetate, and higher propionate molar proportion in the cecum and colon (P<0.05), resulting in lower acetate : propionate ratio (P<0.05). Moreover, there was a 61% decrease in Methanobrevibacter species in the upper colon (P<0.05) and a 19% increase in the acetogen community in the cecum (P<0.05) of treated pigs. Results of our study concluded that supplementation of S. cerevisiae YST2 at 3 g/kg substantially decreased enteric CH4 production in pigs.

Enteric methane mitigation in sheep through leaves of selected tanniniferous tropical tree species

This study was carried out to investigate the effect of supplementation of three selected tanniniferous tropical tree leaves of Ficus benghalensis, Artocarpus heterophyllus and Azadirachta indica on enteric methane emission, rumen fermentation characteristics and protozoal population in 20 native Indian adult male sheep (Mandya). The experiment was conducted in randomized block design with four treatments of five animals each. The sheep in control group (CON) were fed on complete feed block (CFB) comprising finger millet straw and concentrate in the ratio of 60:40 (DM basis) without any inclusion of tropical tree leaves. Sheep in three test groups i.e. FIC, ART and AZA were also fed complete feed block prepared from finger millet straw and concentrate in the same ratio (60:40) as control, but wheat bran in concentrate mixture of test groups was partially replaced (10 parts w/w) with selected tanniniferous tropical tree leaves of Ficus benghalensis, Artocarpus heterophyllus and Azadirachta indica, respectively. The condensed tannin intake in sheep between 7.15 and 10.8g/kg dry matter intake via selected tanniniferous tropical tree leaves did not influence dry matter and digestibility. About 20–26% reduction (p<0.05) in enteric methane emission is achievable simply through the supplementation of selected tree leaves in straw based diet. The reduction in protozoal numbers (p<0.05) due to selected tropical leaves supplementation appeared as primary cause for the reduction in enteric methane emission. The study confirmed that entodinimorphs protozoa are more vulnerable to the condensed tannin supplementation. The study established that significant reduction in methane emission is feasible without affecting feed fermentation characteristics. However, studies in large productive (growing, lactating) ruminants are warranted in order to explore the persistency of ameliorative effect in long term and improvement in productive performance thereof.

Feed intake, growth, and body and carcass attributes of feedlot steers supplemented with two levels of calcium nitrate or urea1

Nitrate supplementation has been shown to be effective in reducing enteric methane emission from ruminants, but there have been few large-scale studies assessing the effects of level of nitrate supplementation on feed intake, animal growth, or carcass and meat quality attributes of beef cattle. A feedlot study was conducted to assess the effects of supplementing 0.25 or 0.45% NPN in dietary DM as either urea (Ur) or calcium nitrate (CaN) on DMI, ADG, G:F, and carcass attributes of feedlot steers ( = 383). The levels of NPN inclusion were selected as those at which nitrate has previously achieved measurable mitigation of enteric methane. The higher level of NPN inclusion reduced ADG as did replacement of Ur with CaN ( < 0.01). A combined analysis of DMI for 139 steers with individual animal intake data and pen-average intakes for 244 bunk-fed steers showed a significant interaction between NPN source and level ( = 0.02) with steers on the high-CaN diet eating less than those on the other 3 diets ( < 0.001). Neither level nor NPN source significantly affected cattle G:F. There was a tendency ( = 0.05) for nitrate-supplemented cattle to have a slower rate of eating (g DMI/min) than Ur-supplemented cattle. When adjusted for BW, neither NPN source nor inclusion level affected cross-sectional area of the LM or fatness measured on the live animal. Similarly, there were no significant main effects of treatments on dressing percentage or fat depth or muscling attributes of the carcass after adjustment for HCW ( > 0.05). Analysis of composited meat samples showed no detectable nitrates or nitrosamines in raw or cooked meat, and the level of nitrate detected in meat from nitrate-supplemented cattle was no higher than for Ur-fed cattle ( > 0.05). We conclude that increasing NPN inclusion from 0.25 to 0.45% NPN in dietary DM and replacing Ur with CaN decreased ADG in feedlot cattle without improving G:F.

Otimização da pecuária nacional de forma sustentável

RESUMO O objetivo deste trabalho foi avaliar o uso de nitrato de cálcio em substituição ao farelo de soja em dieta de ruminantes sobre a mitigação do metano entérico visando determinar os custos com a dieta do animal e os custos marginais de abatimento de carbono. O estudo foi realizado em duas fases, na primeira foi avaliado o potencial de mitigação de metano do nitrato por produção de gases in vitro. Os dados de mitigação de metano foram utilizados na segunda fase, que simulou uma propriedade leiteira com três sistemas de alimentação controle, sem nitrato, e sistemas com inclusão de 1,5 e 3% de nitrato na dieta. Foram utilizadas planilhas de cálculo para avaliar os custos e receitas de cada sistema com vendas de crédito de carbono resultantes da redução de metano ao final de 10 anos. O valor presente líquido (VPL) e a taxa interna de retorno (TIR) foram os índices utilizados na avaliação financeira. A venda de crédito de carbono não alterou o VPL, no entanto a inclusão de nitrato a 1,5 e 3% na dieta animal mostrou-se economicamente viável, pois reduziu o valor das dietas (R$ 5,89 e 5,81/vaca/dia, respectivamente) em comparação com a dieta controle (R$ 6,13 /vaca/dia), além de contribuir para redução de gases de efeito estufa. Portanto, a suplementação de nitrato,como fonte de nitrogênio não protéico, em dietas para ruminantes, além de reduz a produção de metano possibilitou uma sensível redução no custo variável da atividade.

MitiGate; an online meta-analysis database for quantification of mitigation strategies for enteric methane emissions

The body of peer-reviewed papers on enteric methane mitigation strategies in ruminants is rapidly growing and allows for better estimation of the true effect of each strategy though the use of meta-analysis methods. Here we present the development of an online database of measured methane mitigation strategies called MitiGate, currently comprising 412 papers. The database is accessible through an online user-friendly interface that allows data extraction with various levels of aggregation on one hand and data-uploading for submission to the database allowing for future refinement and updates of mitigation estimates as well as providing easy access to relevant data for integration into modelling efforts or policy recommendations. To demonstrate and verify the usefulness of the MitiGate database those studies where methane emissions were expressed per unit of intake (293 papers resulting in 845 treatment comparisons) were used in a meta-analysis. The meta-analysis of the current database estimated the effect size of each of the mitigation strategies as well as the associated variance and measure of heterogeneity. Currently, under-representation of certain strategies, geographic regions and long term studies are the main limitations in providing an accurate quantitative estimation of the mitigation potential of each strategy under varying animal production systems. We have thus implemented the facility for researchers to upload meta-data of their peer reviewed research through a simple input form in the hope that MitiGate will grow into a fully inclusive resource for those wishing to model methane mitigation strategies in ruminants.

Ration Balancing: A Practical Strategy for Reducing Enteric Methane Emission and Carbon Footprint of Milk in India: A Review

Enteric methane is the largest contributor to the total greenhouse gas emissions from agricultural sector. It is closely related to the feed regime and the productivity of dairy animals. Life cycle assessment study indicated that the extensive dairy production system with low milk yields has higher carbon footprint of milk than more intensive system with higher milk yields. In this paper, various methane mitigation strategies have been discussed under Indian feeding and management conditions. These strategies are mainly focused on feeding, breeding and rumen manipulation which can be broadly grouped into those that increase animal productivity and those that directly modify rumen fermentation so that less methane is produced in total. However, most of these strategies are not practically and commercially applicable for reducing methane emissions in large ruminants under field conditions owing to their various limitations. Further, many of these strategies require years of research before practical application and commercially viable products or technologies are available. For India, enteric methane mitigation strategy should be cost-effective and address socio-economic issues. Ration balancing helps in improving productivity and reducing enteric methane emissions in cows and buffaloes at farmers’ doorstep. A life cycle assessment study of milk production in India indicated that a significant reduction in carbon footprint of milk has been observed after feeding a balanced ration. Thus, improving animal productivity through ration balancing with available feed resources could be a most practical strategy for reducing enteric methane emissions and carbon footprint of milk production in India.

Effects of encapsulated nitrate on enteric methane production and nitrogen and energy utilization in beef heifers1,2

The objective of this study was to investigate effects of encapsulated nitrate (EN) on enteric methane emission and N and energy utilization in beef heifers. Eight ruminally-cannulated beef heifers (451 ± 21 kg BW) were used in a replicated 4 × 4 Latin square design. Four experimental diets were prepared and fed once daily for ad libitum intake: control, 1%, 2%, and 3% EN (0.15, 0.9, 1.5, and 2.5% NO3(-) in dietary DM, respectively). The control diet (55% forage and 45% concentrate) included encapsulated urea, which was gradually replaced with EN for the EN diets (iso-nitrogenous; 12.5% CP). In each period, EN was increased stepwise by 1% every 4 d during adaptation. A 7-d washout period (control diet offered to all heifers) was provided between experimental periods. Dry matter intake tended to decrease (10.4 to 10.1 kg/d; linear, P = 0.06) with EN levels. Enteric methane yield was linearly decreased (21.3 to 17.4 g/kg DMI; P < 0.01) by EN, and methane production (g/d) recovered to the level from heifers fed the control diet on the first day when EN was withdrawn from the diet. Apparent total-tract digestibility of DM and OM increased (P = 0.03) or tended to increase (P = 0.06), respectively, with EN levels. Starch digestibility tended to be greater (P = 0.07) for EN vs. The concentrations of rumen ammonia-N and plasma urea-N decreased linearly (P < 0.01) with EN. Total urinary N and urea-N excretion as proportions of N intake were linearly decreased (46.3 to 41.4%, = 0.09 and 37.1 to 29.9%, P = 0.01, respectively) with EN addition. However, NO3(-)-N excretion in urine increased linearly (P < 0.01) with EN levels. Fecal N excretion was not affected (P = 0.47) by EN, although fecal NO-N excretion increased linearly (P < 0.01) with inclusion of EN (0.09 to 0.88% of total N, P < 0.01). Retained N tended to be increased (percentage of N intake; 16.6 to 21.4%, = 0.08) by the EN. Supplementary EN lowered (6.64 to 5.46% of GE intake [GEI], P < 0.01) energy losses by enteric methane mitigation, which increased ME supply (calculated; 56.5 to 58.8% of GEI, P = 0.01) without changes in calculated heat production (P = 0.24). As a result, retained energy tended to increase (P = 0.07) with EN levels. In conclusion, feeding EN to beef heifers lowered enteric methane production in a dose-response manner, which slightly increased energy supply. Total urinary N excretion was lowered for EN due to lower urinary urea-N excretion.

Effects of concentrate supplementation on enteric methane emissions and milk production of grazing dairy cows

Although concentrate supplements in ruminant diets have been recognised as an effective enteric methane mitigation strategy, very few studies have examined the effects of concentrate supplementation on enteric methane emissions under grazing conditions. Twenty four multiparous Holstein Friesian cows were used in a crossover design study to investigate the effects of two concentrate feeding levels across two periods on enteric methane emissions and milk production of grazing dairy cows. Each period had a duration of four weeks (three weeks for diet adaptation and one week for measurements) and no interval in between them. Dietary treatments consisted of two concentrate feeding levels per cow (1 vs. 5kg; as-fed basis) offered daily in equal meals during milking. Enteric methane emissions from cows grazing perennial ryegrass pasture were measured during the final week of each period using the sulphur hexafluoride tracer technique. Milk yield and liveweight were determined daily during each methane measurement period, whereas milk composition and body condition score (BCS) were determined weekly. Daily herbage intake by individual cows during methane measurement weeks was estimated using an energy requirement model and animal records and diet composition. In period 1, cows receiving 5kg concentrate supplement were estimated to reduce herbage intake by 1.8kg DM/d compared to cows receiving 1kg of concentrate, whereas in period 2 cows receiving the 5kg concentrate supplementation were estimated to reduce herbage intake by 4.4kg DM/d, compared to cows receiving 1kg of concentrate. In both periods, milk yield increased with increasing concentrate level, with an average milk response to concentrate supplementation of 0.68kg milk DM/kg concentrate DM over the two periods. Concentrate feeding level had no effect on milk fat, protein or total solids contents. In period 2, lactose content increased in cows offered 5kg/d concentrate. Increasing concentrate feeding level increased liveweight and BCS in period 1, but not in period 2. Feeding 5kg of concentrate supplement increased enteric methane emission by 34g/d in period 1 (323 vs. 357g/d) and 41g/d in period 2 (349 vs. 390g/d) compared to 1kg of concentrate supplement. However, enteric methane emission per unit of estimated feed intake (dry matter or gross energy) or milk output (gross or energy corrected) was not affected by level of concentrate supplementation. It was concluded that under generous grazing conditions (high allowance of good quality herbage) a moderate increase in concentrate supplementation resulted in a simultaneous increase in milk yield and enteric methane emission, so that enteric methane emission per unit of milk yield was unaffected. Thus, a moderate level of concentrate supplementation of dairy cows grazing pastures of high digestibility would not be an effective enteric methane mitigation strategy.

Utilization of Saponins, a Plant Secondary Metabolite in Enteric Methane Mitigation and Rumen Modulation

Methane (CH4) emission originated from the livestock is a major concern globally as it is a potent greenhouse gas and also accounts for 3-10% loss of ingested feed energy for productive purposes. Interventions for CH4 mitigation based on plant secondary metabolites (PSM) have been considered as safe, economical and effective strategies. Saponins, a multifunctional PSM, exhibited immense medicinal importance in traditional medicine system as well as in experimental biological systems. Last few decades witnessed a steady increase in interest towards application of saponins as rumen fermentation modulating agent as well a promising methane inhibitor. Saponins containing plants and their purified saponins have shown encouraging results in in vitro and in vivo experimental conditions. However, further studies are warranted to evaluate their toxicity profile, metabolism and mechanism of action at molecular level. This review focuses on the current status of role of saponins in ruminant nutrition.