Entamoeba Moshkovskii Research Articles

Molecular identification of entamoeba Dispar and entamoeba Moshkovskii by using nested multiplex PCR in symptomatic indivisuals

The current study was for determine Entamoeba dispar & Entamoeba moshkovskii in symptomatic individuates by molecular identification ( nested multiplex PCR ) in order obtained for an accurate diagnosis of these Entamoeba species. Total stool samples (120) that collected from symptomatic patients with diarrhea. These samples were collected from patients according to age, residency during the period from ( the first of November 2021 to the end of January 2022). All samples undergo for a direct microscopic examination, The results revealed that 100(73.18%)were positive samples with Entamoeba spp. The results of microscopic examination recorded high infection rate in age group in (4-10)years83.33%. In contrast, recorded high prevalence in rural areas 76.31% than urban areas 73.68% . Showed the method nested multiplex polymerase chain reaction by using 18s rRNA small subunit gene, the first round of PCR showed the infection rate by Entamoeba spp 87(65.41%) out of 120 stool samples .The second round of nested multiplex PCR for these positive samples by using two specific primers revealed mix infection of Entamoeba dispar & Entamoeba moshkovskii with Entamoeba histolytica 5(6.25%), 2(2.5%) respectively, with significant difference at P<0.05.

Molecular Identification and Prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, Northern Iraq.

The present study was conducted to evaluate the infection rates of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii among asymptomatic individuals in Erbil City, northern Iraq. The research intent was to discover whether pathogenic or nonpathogenic species cause a high rate of symptomless Entamoeba infections. Stool samples were microscopically examined, and the 18S-rRNA gene was targeted utilizing the nested PCR technique in the positive specimens. Initial results based on morphological features showed that the Entamoeba prevalence rate was 7.4%. Significantly higher rates of infections were seen in females than in males and in low-income people than in moderate-income people. The incidence rates among the asymptomatic individuals, as determined by molecular analysis, were as follows: E. histolytica - 6%, E. dispar - 4.3%, and E. moshkovskii - 0.3%. Of all the Entamoeba positive samples, a single infection with E. histolytica was identified in 41.4% samples; the single infection with E. dispar in 18.6% samples, 35.7% samples had mixed infections with two Entamoeba species, and 4.3% had mixed infections with three species. The current study concluded that 7.4% of healthy people, who live in the endemic area under investigation, carry Entamoeba species asymptomatically. Additionally, the majority of asymptomatic Entamoeba infections were caused by the pathogenic E. histolytica (81.4%) compared to E. dispar (58.6%), and E. moshkovskii with the lowest rate of infection. Single and co-infections with E. histolytica and E. dispar were noted. E. moshkovskii, which was identified for the first time in the region, was only seen in mixed infections. The present study was conducted to evaluate the infection rates of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii among asymptomatic individuals in Erbil City, northern Iraq. The research intent was to discover whether pathogenic or nonpathogenic species cause a high rate of symptomless Entamoeba infections. Stool samples were microscopically examined, and the 18S-rRNA gene was targeted utilizing the nested PCR technique in the positive specimens. Initial results based on morphological features showed that the Entamoeba prevalence rate was 7.4%. Significantly higher rates of infections were seen in females than in males and in low-income people than in moderate-income people. The incidence rates among the asymptomatic individuals, as determined by molecular analysis, were as follows: E. histolytica – 6%, E. dispar – 4.3%, and E. moshkovskii – 0.3%. Of all the Entamoeba positive samples, a single infection with E. histolytica was identified in 41.4% samples; the single infection with E. dispar in 18.6% samples, 35.7% samples had mixed infections with two Entamoeba species, and 4.3% had mixed infections with three species. The current study concluded that 7.4% of healthy people, who live in the endemic area under investigation, carry Entamoeba species asymptomatically. Additionally, the majority of asymptomatic Entamoeba infections were caused by the pathogenic E. histolytica (81.4%) compared to E. dispar (58.6%), and E. moshkovskii with the lowest rate of infection. Single and co-infections with E. histolytica and E. dispar were noted. E. moshkovskii, which was identified for the first time in the region, was only seen in mixed infections.

Entamoeba moshkovskii Tshalaia, 1941 en Costa Rica

The finding of Entamoeba moshkovskii Tshalaia, 1941 in the sewage digestion tanks of the City of Cartago, Costa Rica is mentioned. A description of the culture media and the fixation technique employed in the observation of stained preparations is given. A brief description of the strain of E. moshkovskii isolated 10 Costa Rica, together with some photomicrographs, is presented by the author. E. moshkovskii is considered as a good species by the author, not believing that this is a case of a free-living phase of sore parasitic entamoeba with tetranucleate cysts.

Detection of entamoeba dispar and entamoeba moshkovskii DNA in liver abscess pus: Newer perspectives to be considered in diagnosis of amoebiasis

Detection of entamoeba dispar and entamoeba moshkovskii DNA in liver abscess pus: Newer perspectives to be considered in diagnosis of amoebiasis

Correction: Species-Specific Immunity Induced by Infection with Entamoeba histolytica and Entamoeba moshkovskii in Mice

Correction: Species-Specific Immunity Induced by Infection with Entamoeba histolytica and Entamoeba moshkovskii in Mice

Ultrastructure of Entamoeba moshkovskii with particular regard to the presence of surface active lysosomes

Ultrastructure of Entamoeba moshkovskii with particular regard to the presence of surface active lysosomes