Molecular identification of entamoeba Dispar and entamoeba Moshkovskii by using nested multiplex PCR in symptomatic indivisuals

Abstract

The current study was for determine Entamoeba dispar & Entamoeba moshkovskii in symptomatic individuates by molecular identification ( nested multiplex PCR ) in order obtained for an accurate diagnosis of these Entamoeba species. Total stool samples (120) that collected from symptomatic patients with diarrhea. These samples were collected from patients according to age, residency during the period from ( the first of November 2021 to the end of January 2022). All samples undergo for a direct microscopic examination, The results revealed that 100(73.18%)were positive samples with Entamoeba spp. The results of microscopic examination recorded high infection rate in age group in (4-10)years83.33%. In contrast, recorded high prevalence in rural areas 76.31% than urban areas 73.68% . Showed the method nested multiplex polymerase chain reaction by using 18s rRNA small subunit gene, the first round of PCR showed the infection rate by Entamoeba spp 87(65.41%) out of 120 stool samples .The second round of nested multiplex PCR for these positive samples by using two specific primers revealed mix infection of Entamoeba dispar & Entamoeba moshkovskii with Entamoeba histolytica 5(6.25%), 2(2.5%) respectively, with significant difference at P<0.05.