Molecular investication of entamoeba histolytica by nested multiplex PCR in diarrheoginic patients

Abstract

The current study was for determine Entamoeba histolytica in diarrheal patients by molecular investigation (nested multiplex PCR) in order obtained for an accurate diagnosis of Entamoeba histolytica that causes amoebiasis. Total stool samples (133) that collected from symptomatic patients with diarrhea according to age, gender. Then all samples undergo for a direct microscopic examination, The result revealed that 100(73.18%) were positive samples with Entamoeba spp. The results of microscopic examination revealed a higher infection rate of Entamoeba spp were in the female 83.87% compared the male 67.6% with significant difference at P<0.05 ,and recorded high infection rate in age group in (4-10)years and age group (more than 50)years 83.33%,86.66% respectively, and lower infection rate in age group (>1)year 55.55% .Showed the method nested multiplex polymerase chain reaction by using 18s rRNA small subunit gene, nested multiplex PCR for these positive samples by using specific primer for E. histolytica revealed a higher infection rate was E. histolytica 73(91.25%) (monoinfection). Entamoeba histolytica high infection rate recorded in the female 97.43% compared the male 82.35%. In addition, E. histolytica recorded the more percentage infection was in age group (less than one year) and (31-50)years was scored 3(100%), 11(100%), respectively.