AbstractDoppler radar data are assimilated with an ensemble Kalman Filter (EnKF) in combination with a double-moment (DM) microphysics scheme in order to improve the analysis and forecast of microphysical states and precipitation structures within a mesoscale convective system (MCS) that passed over western Oklahoma on 8–9 May 2007. Reflectivity and radial velocity data from five operational Weather Surveillance Radar-1988 Doppler (WSR-88D) S-band radars as well as four experimental Collaborative and Adaptive Sensing of the Atmosphere (CASA) X-band radars are assimilated over a 1-h period using either single-moment (SM) or DM microphysics schemes within the forecast ensemble. Three-hour deterministic forecasts are initialized from the final ensemble mean analyses using a SM or DM scheme, respectively. Polarimetric radar variables are simulated from the analyses and compared with polarimetric WSR-88D observations for verification. EnKF assimilation of radar data using a multimoment microphysics scheme for an MCS case has not previously been documented in the literature. The use of DM microphysics during data assimilation improves simulated polarimetric variables through differentiation of particle size distributions (PSDs) within the stratiform and convective regions. The DM forecast initiated from the DM analysis shows significant qualitative improvement over the assimilation and forecast using SM microphysics in terms of the location and structure of the MCS precipitation. Quantitative precipitation forecasting skills are also improved in the DM forecast. Better handling of the PSDs by the DM scheme is believed to be responsible for the improved prediction of the surface cold pool, a stronger leading convective line, and improved areal extent of stratiform precipitation.