Enrichment Of Fatty Acids Research Articles

Enrichment of Polyunsaturated fatty acids from catfish oil with immobilized Rhizopus oryzea lipase

Nowadays, Pangasius hypophthalmus fillet is one of the main marine products in Vietnamese Mekong River Delta with over three hundred thousand tons per year. One of the byproducts of this industry is fat, about one hundred thousand tons per year. That byproduct has been only used in animal foodstuff industry after oil extracting or it becomes waste. In order to increase the quality of Pangasius hypophthalmus oil, we have enriched PUFA in the oil by hydrolysis with immobilized Rhizopus oryzea lipase. Lipase has been immobilized on celite by absorption method. Immobilization experiments have been designed in a two-level full factorial model with three factors. The fish oil has been hydrolyzed with immobilized lipase to enrich PUFA with optimized parameters found out: the ratio
 of enzyme to support of 1:10 g/g, the ratio of phosphate buffer volume to support weight of 30.55 ml/g, incubation time of 5.15 hours. The result has showed that 60-minute hydrolysis is the most appropriate. Additionally, the oil iodine value has increased from 71.4 to 97.4, and DHA concentration has risen from 1156mg/kg to 1916mg/kg.

Plasma BDNF is a more reliable biomarker than erythrocyte omega-3 index for the omega-3 fatty acid enrichment of brain

Enriching brain DHA is believed to be beneficial for the prevention and treatment of several neurological diseases, including Alzheimer’s disease. An impediment in assessing the effectiveness of the treatments is the lack of a reliable biomarker for brain DHA. The commonly used erythrocyte omega-3 index is not suitable for brain because of the involvement of unique transporter at the blood brain barrier (BBB). We recently showed that dietary lysophosphatidylcholine (LPC)-DHA significantly increases brain DHA, which results in increase of brain BDNF. Since there is bidirectional transport of BDNF through the BBB, we tested the hypothesis that plasma BDNF may be used as biomarker for brain DHA enrichment. We altered the brain DHA in rats and mice over a wide range using different dietary carriers of DHA, and the correlations between the increase in brain omega-3 index with the increases in plasma BDNF and the erythrocyte index were determined. Whereas the increase in brain omega-3 index positively correlated with the increase in plasma BDNF, it negatively correlated with the erythrocyte index. These results show that the plasma BDNF is more reliable than the erythrocyte index as biomarker for assessing the effectiveness of omega-3 supplements in improving brain function.

Development of Industrially Scalable Method for Phospholipids and Branch‐Chain Fatty Acids of Dairy by‐Product

AbstractDairy phospholipids (PL) is of interest due to their health benefits and functional properties. Solvent fractionation of PL has not been commonly used in the dairy industry to fractionate milk fat to obtain or concentrate the PL. The total lipid extracted from the β stream, a waste by‐product of dairy processing, was used in this study to investigate suitable solvent and conditions to separate the neutral lipid from PL. A fixed lipid solvent ratio (1:10 g/v) was used at various fractionation temperatures (−20, 2, 15, 23, 40, and 60 °C) depending on the solvents. The use of acetone at 23 °C, not at the lower temperatures, led to a dairy lecithin product with high PL content, such as 71.5%. The more aqueous ethanol, i.e. at 70% concentration compared to 95%, was able to preferentially extract PL to form products with up to 74.7% PL, but the PL yield was much lower (26.3%) compared to acetone precipitation (97.9%). The enrichment of branch chain fatty acids proved to be very challenging due to the overlapping melting points with other fatty acids. The composition of the major fatty acids of polar and neutral lipids also showed interesting patterns that may indicate different nutritional and oxidative properties of the fractionated products.

Time course of nutritional and functional property changes in egg yolk from laying hens fed docosahexaenoic acid-rich microalgae

Marine microalgae (MA) has received wide attention as a promising source of omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) enrichment in animal products to improve the health status and wellbeing of the consumers. This study evaluated dynamic changes in n-3 LC-PUFA, color, and functional properties as well as atherogenic and thrombogenic health lipid indices of egg yolk from hens fed graded levels (0, 0.5, and 1.0%) of docosahexaenoic acid (DHA)–rich MA (Aurantiochytrium sp.) during a 56-D feeding period. Egg freshness parameters and yolk lipid oxidative stability were also measured after 0, 14, and 28 D of refrigerated storage. The hen performance and egg quality (except for yolk color) were not affected (P > 0.05) by MA supplementation. Docosahexaenoic acid contents in yolk from hens fed 1.0% MA increased quadratically with feeding time with a plateau at about 30 D (P < 0.05). Afterward, the DHA content leveled off to a constant value (946.3 mg/100 g yolk) with the n-6/n-3 ratio at 3.5: 1. Dietary inclusion of 1.0% of MA also significantly decreased the atherogenic and thrombogenic indices of yolk lipid (P < 0.05). Because the microalgal carotenoids incorporated into egg yolk, the L∗ value of yolk from hens fed MA decreased whereas a∗ value increased (P < 0.05), corresponding to yolk Roche color scores. As expected, there were no significant changes in yolk functional properties (e.g., viscosity and emulsifying activity) related to DHA enrichment (P > 0.05). Microalgal carotenoids enrichment also helped attenuate fatty acid oxidation of the DHA-enriched yolk and increase their lipid oxidative stability. In conclusion, dietary supplementation with up to 1.0% of MA significantly increased DHA contents with more health-promoting n-6/n-3 ratio and atherogenic and thrombogenic indices, as well as more intense yolk color within consumers' acceptability, and the feeding strategy had a minimal impact on yolk physical and functional properties or oxidative stability during subsequent refrigerated storage.

13C analysis of fatty acid fragments by gas chromatography mass spectrometry for metabolic flux analysis

When multiple metabolic pathways lead to the same product, compound-specific isotope analysis may not provide enough information to quantify the activities of the contributing pathways. Instead, identification of where in the molecule the 13C is incorporated is required. Here we show how knowledge of position-specific 13C incorporation in fatty acids (FA) and FA fragments can be used to quantitatively estimate the fluxes through the central C metabolic network.We developed a method to measure 13C enrichment of FA and FA fragments (ethanoate, propionate) using electron impact GC–MS. We tested the accuracy and repeatability of the measurements using natural abundance and position-specific 13C labelled standards and FA extracted from Bacillus licheniformis and Pseudomonas fluorescens grown with labelled and unlabelled glucose. The molecular ions of FA generally reflected theoretical predictions of mass isotopomer distributions for natural abundance values, but that of the associated FA fragments deviated from expected values, likely associated with McLafferty rearrangements of hydrogen. After correction for naturally occurring isotopes, 13C enrichments of FA and FA fragments showed good agreement with expected isotope composition of FA standards (root mean square error < 0.044 at%; δ13C of ∼ 40‰), natural abundance and labelled glucose. The unsaturated FA extracted from P. fluorescens deviated from expected values likely associated with problems of co-elution and ion suppression and were excluded from analysis.The ratio of glucose-1-13C to glucose-3-13C incorporation into FA fragments was high for B. licheniformis, but low for P. fluorescens. Metabolic flux modelling based on the 13C enrichment of ethanoate and propionate fragments showed that B. licheniformis used Embden-Meyerhof-Parnas and pentose phosphate pathway (66% and 30%, respectively), whereas P. fluorescens utilized Entner-Doudoroff and pentose phosphate pathway (72% and 27%, respectively). FA fragment analysis is therefore a promising tool to study central C metabolic network activities of co-occurring groups of microbes in intact and complex environmental communities.

Effect of Using Moringa oleifera and Spirulina platensis as Feed Additives on Performance, Meat Composition and Oxidative Stability and Fatty Acid Profiles in Broiler Chicken

A study was conducted to determine mainly the n 3 fatty acids enrichment and decreased oxidation of broiler meat using the plant Moringa oleifera and a blue green algae Spirulina platensis. The effects of the dietary treatments were evaluated in terms of growth performance, carcass and meat yields, oxidative stability and fatty acids modulation. Two hundred and forty (240) one day old Cobb 500 broiler chicks were assigned to 5 dietary treatments for 5 weeks, with 4 replications having 12 chicks per replication. The diets of the treatments were formulated from the basal feed as follows: control (T1), including 2 different levels of M. oleifera leaf meal 1% (T2) and M. oleifera leaf meal 1.5%, (T3) and 2 different levels of S. platensis 1% (T4) and S. platensis 1.5% (T5). The birds were randomly distributed in each pen, and the data were analyzed using the SPSS statistical package. The final body weight (BW) gain was significantly higher in the T2 and T5 groups, and the feed conversion ratio improved in the T2 group (1.68). The lowest (p ≤ 0.05) thiobarbituric acid-reactive substances values (TBARS) of breast and thigh were obtained in T2 groups compared to T3-T5 group after the second week of preservation. Regarding fatty acid profile of breast and thigh meat, the omega-3 fatty acid levels, such as those of linolenic and docosahexaenoic acid (DHA), were increased in the additives groups. The results of the present study elucidated that dietary inclusion of the 2 medicinal plants in the T2 and T5 groups could be promising functional ingredients to produce value-added broiler meat in terms of oxidative stability and omega-3 fatty acids enhancement.

Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity.

Enrichment of omega-3 fatty acids (ɷ-3 FAs) in natural oils is important to realize their health benefits. Lipases are promising catalysts to perform this enrichment, however, fatty acid specificity of lipases is poor. We attempted to improve the fatty acid selectivity of a lipase from Geobacillus thermoleovorans (GTL) by two approaches. In a semi-rational approach, amino acid positions critical for binding were identified by docking the substrate to the GTL and best substitutes at these positions were identified by site saturation mutagenesis followed by screening to obtain a variant of GTL (CM-GTL). In the second approach based on rational design, a variant of GTL was designed (DM-GTL) wherein the active site was narrowed by incorporating two heavier amino acids in the lining of acyl-binding pocket to hinder access to bulky ɷ-3 FAs. The affinities DM-GTL with designed substrates were evaluated in silico. Both, CM-GTL and DM-GTL have shown excellent ability to discriminate against the ɷ-3 FAs during hydrolysis of oils. Engineering the binding pocket of an enzyme of a complex substrate, such as a triglyceride, by incorporating the information on substrate structure and computationally derived binding modes, has resulted in designing two efficient lipase variants with improved substrate selectivity.

Effects of feeding broiler breeder hens a coextruded full-fat flaxseed and pulses mixture without or with multienzyme supplement

The effects of coextruded full-fat flaxseed and pulses (FFF; 1:1 wt/wt) mixture on n-3 polyunsaturated fatty acids (PUFA) enrichment in egg yolk, hepatic attributes, apparent retention (AR) of components, and ceca metabolites were evaluated in broiler breeder hens. The diets were as follows: 1) corn–soybean control, 2) control diet plus 18% FFF (FFF−), and 3) FFF plus enzyme supplement (FFF+) containing galactanase, protease, mannanase, glucanase, xylanase, amylase, and cellulase activities. Twenty-six-week-old Cobb 500 broiler breeder hens were allocated to 30 identical cages (2 hens/cage) and given 1-week adaptation period. The 3 diets were assigned to 10 replicate cages based on postadaptation BW and fed based on breeder curve for 30 D. Excreta samples were collected from day 24 to 27 for determination of AR of components, and eggs were collected from day 28 to 30 for yolk polyunsaturated fatty acids analyses. On day 30, birds were weighed, killed via cervical dislocation, liver weighed, and stored for fat analyses. Ceca digesta samples were taken for concentration of short-chain fatty acids. Liver and yolk weights as well as total yolk FA were not influenced by diets (P > 0.05). Control birds had lower yolk concentration of α-linolenic acid than birds fed either FFF− or FFF+ (P < 0.01) corresponding to 7.5, 36.8, and 37.3 mg/g for the control, FFF−, and FFF+, respectively. Control birds also exhibited lower yolk concentration of docosahexaenoic acid (P < 0.01). Control birds had higher hepatic concentration of crude fat and apparent retention of dry matter and crude protein compared with either the FFF− or FFF+ birds (P < 0.05). Birds fed FFF- diet had lower ceca digesta concentration of lactic acid than control and FFF+ (P < 0.05) birds. Results showed broiler breeder hens enriched egg yolk with n-3 polyunsaturated fatty acids without effects on the liver while the supplemental enzyme did not improve the utilization of FFF.

Arginine-fed cultures generates triacylglycerol by triggering nitrogen starvation responses during robust growth in Chlamydomonas

Under nitrogen (N) starvation, certain microalgae increase carbon storage in the form of lipid droplets while also downregulating photosynthesis and eventually terminating growth. To improve lipid yield, we asked whether lipid droplets and N starvation responses can be induced without limiting growth or photosynthesis. In the chlorophyte Chlamydomonas reinhardtii, N starvation induces gametogenesis alongside lipid droplet accumulation, and gametogenesis has been observed in arginine-fed cultures wherein the arginine was provided as the sole N source. We therefore assessed whether arginine-fed cultures displayed N starvation responses other than gametogenesis in mixo- and phototrophic conditions, representing two primary modes of nutrition that may affect N starvation responses. We showed that arginine-fed cultures using two lab strains of C. reinhardtii supported normal mixotrophic growth, constitutively turned on N starvation-induced genes at the equivalent level to N-starved cells, and increased the triacylglycerol content of total fatty acids by 125–400% relative to ammonium-fed cultures. The lipid profile of triacylglycerol in these arginine-fed mixotrophic cultures exhibited 3 to 5.5-fold enrichment of saturated and monounsaturated fatty acids, a preferred characteristic of biodiesel precursors. Arginine-fed phototrophic cultures likewise turned on N starvation-induced genes, accumulated lipid droplets, and led to a 50% reduced growth rate per day while reaching a 3 to 6.5-fold more cell density at the stationary phase relative to ammonium-fed cultures. To test the applicability of our result to algae outside the green lineage, we conducted similar experiments with the diatom Phaeodactylum tricornutum, which also accumulated 2 to 7-fold more neutral lipids in arginine-fed phototrophic cultures without growth impairment relative to nitrate-fed cultures. We document a system wherein N starvation responses are induced without compromising photosynthesis or growth, thereby suited to the production of valuable chemicals and biofuel precursors without requiring stressors in microalgae.

Comparative effects of dietary microalgae oil and fish oil on fatty acid composition and sensory quality of table eggs

This study was conducted to investigate the comparative effects of dietary supplementation with microalgae oil or fish oil on fatty acid composition, sensory quality, and overall acceptability of table eggs. A total of six hundred thirty, 30-week-old, Hy-Line Brown laying hens were allocated to 7 dietary treatments. Layers were fed with a control diet or the control diet supplemented with graded levels of docosahexaenoic acid (DHA; 1.25, 2.50, and 5.00 mg/g feed) derived from microalgae oil or fish oil. The feeding trial lasted 10 wk. Enrichment of eggs with DHA by dietary supplementation with microalgae oil or fish oil enhanced yolk DHA and total n-3 polyunsaturated fatty acid (PUFA) enrichment and reduced n-6-to-n-3 ratio in a dose-response manner, whereas the efficiency decreased (P < 0.05). The overall efficiency of DHA incorporation into eggs was similar for the 2 sources (P > 0.05). In comparison with fish oil, dietary microalgae oil supplementation resulted in higher scores for egg flavor and overall acceptability, both of which declined linearly in response to DHA supplementation (P < 0.05). Among the aroma and flavor characteristics, fishy aroma and flavor scores increased linearly and quadratically (P < 0.05) in response to dietary DHA supplementation, and egg aroma and flavor and milky flavor scores decreased linearly (P < 0.05). The results from partial least squares analysis showed that fishy flavor and aroma were closely associated with DHA, α-linolenic acid, and total n-3 PUFA, and oleic acid, arachidonic acid, and dihomo-gamma-linolenic acid were more related to egg aroma and flavor. The results suggested that microalgae oil would be more promising for egg DHA enrichment owing to better sensory quality of the resultant eggs.

Transfer of glucose hydrogens via acetyl-CoA, malonyl-CoA, and NADPH to fatty acids during de novo lipogenesis

Deuterated water (2H2O) is widely used for measuring de novo lipogenesis (DNL). 2H is incorporated into fatty acids via exchange between body water and the hydrogens of acetyl-CoA, malonyl-CoA, and NADPH. Previous studies concluded that these exchanges are incomplete; therefore, fatty acid 2H enrichment requires correcting. In mice, we measured the 2H enrichment of fatty acid positions 2 and 3 and methyl hydrogens from [U-2H7]glucose to determine 2H transfer from glucose to fatty acid via malonyl-CoA, NADPH, and acetyl-CoA, respectively. Positional fatty acid 2H enrichments were compared with 13C enrichment of the same sites from an equivalent amount of [U-13C6]glucose provided alongside the [U-2H7]glucose tracer. Transfer of glucose 2H to fatty acid position 2 and methyl sites was low (2H enrichment of 0.06 ± 0.01 and 0.14 ± 0.01 relative to 13C) indicating extensive exchange at both malonyl- and acetyl-CoA, respectively. Transfer of glucose 2H into fatty acid position 3 was more extensive (0.46 ± 0.04 relative to 13C, P < 10-5 vs. position 2), indicating a more limited exchange of those glucose hydrogens that were transferred via NADPH. However, mice provided with [U-13C6]glucose and 2H2O had equivalent 2H enrichments of fatty acid positions 2 and 3, suggesting that in this setting, NADPH and body water 2H had exchanged extensively. This is explained by contributions of substrates other than exogenous glucose to DNL coupled with their extensive 2H enrichment from 2H2O prior to DNL. Under such conditions, 2H enrichment of fatty acids from 2H2O does not need correction.

Enriching Artemia nauplii with selenium from different sources and interactions with essential fatty acid incorporation

The production of high-quality marine fish fry is limited by the low survival observed during the larval phase, which is often attributed to dietary deficiencies of the diets at first feeding. Despite progress made with live feed (i.e. rotifers, Artemia), enrichments in essential fatty acids for marine fish larvae, little is known on the micronutrient requirements such as selenium (Se). Se is a critical component of several enzymes maintaining important biological functions such as cellular oxidation, and therefore plays a key role in oxidative and stress status of marine larvae. The levels of Se found in the larvae's natural diet (i.e. copepods) is generally higher than those of the enriched live preys used in hatcheries. This study aimed at establishing a protocol to enrich Artemia nauplii with Se using different inorganic (sodium selenite) and organic (selenoyeast). Results indicated that the use of dissolved sodium selenite, an alternative inorganic and cheaper form of Se, did not increase the levels of Se in the nauplii. However, the use of selenoyeast (Sel-Plex) confirmed that it is possible to enrich the nauplii with targeted levels of Se, since this process followed a dose-response pattern with Se enrichment ranging from 1.7 to 12.4 mg kg−1. In addition, the supplementation of Sel-Plex to the regular enrichment product did not impact on lipids and fatty acids enrichment irrespective of the dose dispensed. Overall, this study contributes to the refinement of the live prey enrichment protocols that are critical to the success of marine finfish larviculture protocols.

FEED ENRICHMENT WITH FISH OIL AND CORN OIL TO INCREASE EEL GROWTH RATE Anguilla bicolor (McCelland, 1844)

Eel (Anguilla bicolor) is an Indonesian freshwater commodity that has high economic value but has slow growth. One method to accelerate eel growth is through feeding with the enrichment of essential fatty acids. The aim of this research is to know the increase of growth rate of eel fish fed with the enrichment of essential fatty acids with fish oil and corn oil. The study used 3 treatments and 3 replications ie A (0% corn oil + 0% fish oil), B (1.5% corn oil + 1.5% fish oil), and C (3% corn oil + 3% fish oil). Parameters observed included specific growth rate, total feed consumption, feed efficiency, fat retention, and survival rate. The supporting parameter is water quality. The data obtained were analyzed by analysis of variance and continued by fisher test with 95% confidence interval. The results showed that there was a significant effect (P &lt;0.05) on the growth rate of eel fish fed with the enrichment of essential fatty acids with fish oil and corn oil. Treatment C (3% corn oil + 3% fish oil) is the optimum dose because it has the best growth performance and best feed efficiency that is specific growth rate (4.67%), total feed consumption (262,9 g), feed efficiency (61% ), fat retention (2.805%), and survival rate (80%).

Enrichment of Erucic and Gondoic Fatty Acids from Crambe and Camelina Oils Catalyzed by Geotrichum candidum Lipases I and II

AbstractErucic (22:1, cisΔ13) and gondoic acids (20:1, cisΔ11) are building blocks obtained from renewable sources for the oleochemical industry. Different biocatalytic strategies for the enrichment of these compounds with high recovery yields were developed in our group. Geotrichum candidum lipases (GCL) strongly discriminate against fatty acids longer than 18 carbon atoms. Thus, GCL‐I and ‐II were investigated using hydrolysis or ethanolysis reactions with Crambe and Camelina oils. Hydrolysis was also studied using fatty acid ethyl esters (FAEE) derived from the corresponding oil. Both isoforms were highly selective; however, interesting differences were observed. Although it has been reported that GCL‐I displays a higher preference toward 18 cisΔ9, which is present in the studied oils at high levels, GCL‐II showed higher enrichment values during hydrolysis independent of the substrate used. Hence, enrichments of 87% (Crambe oil) and 82% (Crambe FAEE) for erucic acid and 50% (Camelina oil) and 45% (Camelina FAEE) for gondoic acid, with recovery values between 89% and 99%, were achieved. On the contrary, the best enzyme for ethanolysis was GCL‐I (82% and 41% for erucic and gondoic acid, respectively). In this case, although GCL‐II also displayed good enrichment and recovery levels (77% and 28%, respectively), they were lower compared to the former reactions. In both ethanolysis reactions, the FAEE fraction contained between 92% and 97% of 18 unsaturated fatty acids.

Enrichment of omega-3 fatty acids, waste oil by-products canning tuna (thunnus sp.) with urea crystallization

Enrichment test of omega-3 fatty acids from tuna canning waste oil in Bitung North Sulawesi Indonesia have been conducted. The purpose of this study was to utilize tuna canning waste oil and enriched the concentration of omega-3 fatty acids with urea crystallization. The results showed that the characteristics of fish oil quality of tuna canning waste 0.9% moisture content, free fatty acids (FFA) 3.4%, copper (Cu) 0.1 ppm, iron (Fe) 2.5 ppm and phosphorus (P) 32,5 ppm still meets IFOMA quality standards Liquid fatty acid profile contains total SFA 33.7%, 27.6% PUFA, 1.4% omega-6 fatty acids, omega-3 fatty acids 25.9%, EPA 4.2% and DHA 21.4%, MUFA 18.8%, omega-9 fatty acids 13.9%, while concentrations of omega-3 fatty acids contained a total SFA of 7.7%, PUFA 70.9%, omega-6 fatty acids 0.8%, omega-3 fatty acids 70.1%, EPA 4.2% and DHA 21.4%, MUFA 2.7%, omega-9 fatty acids 1.5%. The urea crystallization process increases the levels of polyunsaturated fatty acids (PUFAs) such as omega-3 (EPA and DHA.), the enrichment rate is 2.7 times.

Purification and characterization of an extracellular lipase from Trichosporon sp. and its application in enrichment of omega-3 polyunsaturated fatty acids

Lipase catalysis has become the most promising technique for enrichment of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) due to its mild and green conditions. In this study, Trichosporon sp. F1-2 was newly isolated from oil-contaminated sludge, and its extracellular lipase was purified using ammonium sulfate precipitation and DEAE-Sepharose chromatography. The molecular weight of the lipase was estimated to be 32.6 kDa. The optimal temperature and pH for lipase activity were 50 °C and 8.0, respectively, and the optimal storage conditions were found to be temperatures below 45 °C and pH 7.0–9.0. The lipase did not require metal ions as cofactors and was significantly inhibited by Zn2+. It was stable in various organic solvents with different log P values, and n-hexane, toluene, dichloromethane, diethyl ether and glycerol even enhanced its activity. It had sn-1,3 regioselectivity and the highest activity towards a medium-chain fatty acid (C8) ester. This study demonstrated the lipase has good potential as a catalyst in enrichment of EPA and DHA in fish oil. The content of EPA and DHA reached 22.9 and 25.4 mol%, respectively, using 100 U of the lipase within 24 h, and the enrichment efficiency of DHA was higher than that of EPA.

Stearoyl-CoA Desaturase 1 as a Therapeutic Target for the Treatment of Cancer.

A distinctive feature of cancer cells of various origins involves alterations of the composition of lipids, with significant enrichment in monounsaturated fatty acids. These molecules, in addition to being structural components of newly formed cell membranes of intensely proliferating cancer cells, support tumorigenic signaling. An increase in the expression of stearoyl-CoA desaturase 1 (SCD1), the enzyme that converts saturated fatty acids to ∆9-monounsaturated fatty acids, has been observed in a wide range of cancer cells, and this increase is correlated with cancer aggressiveness and poor outcomes for patients. Studies have demonstrated the involvement of SCD1 in the promotion of cancer cell proliferation, migration, metastasis, and tumor growth. Many studies have reported a role for this lipogenic factor in maintaining the characteristics of cancer stem cells (i.e., the population of cells that contributes to cancer progression and resistance to chemotherapy). Importantly, both the products of SCD1 activity and its direct impact on tumorigenic pathways have been demonstrated. Based on these findings, SCD1 appears to be a significant player in the development of malignant disease and may be a promising target for anticancer therapy. Numerous chemical compounds that exert inhibitory effects on SCD1 have been developed and preclinically tested. The present review summarizes our current knowledge of the ways in which SCD1 contributes to the progression of cancer and discusses opportunities and challenges of using SCD1 inhibitors for the treatment of cancer.

FEED ENRICHMENT WITH FISH OIL TO INCREASE EEL GROWTH RATE Anguilla bicolor (McCelland, 1844)

Eel (Anguilla bicolor) is a export commodity fish in fisheries sector and has has high economic value but has slow growth. One method to accelerate eel growth is through feeding with the enrichment of fish oil. The aim of this research is to know the increase of growth rate of eel fish fed with the enrichment of essential fatty acids with fish oil. The study used 3 treatments and 3 replications ie A (0% fish oil / control), B (1.5% fish oil), and C (3% fish oil). Parameters observed included specific growth rate, feed consumption total, feed efiesiensi, fat retention, survival rate. The supporting parameters are water quality. The result of research obtained were tabulated and analyzed by excel 2013 program and SPSS v. 20.0. with 95% confidence interva and continued by Duncan test. The results showed that there was a significant effect (P &lt;0.05) on the growth rate of eel fish fed with the enrichment of essential fatty acids with fish oil. Treatment C (3% fish oil) is the optimum dose because it has the best growth performance and best feed efficiency that is specific growth rate (4,61% ± 0,0075), total feed consumption (246,3 g ± 50,1), feed efiesiensi (42,5% ± 3,74), fat retention (2,97% ± 0,148) and survival rate (86,7% ± 11,5).

Highly Efficient and Enzyme-Recoverable Method for Enzymatic Concentrating Omega-3 Fatty Acids Generated by Hydrolysis of Fish Oil in a Substrate-Constituted Three-Liquid-Phase System.

A novel three-liquid-phase system which contained fish oil as the nonpolar phase was developed for the lipase-based hydrolysis of fish oil and subsequent enrichment of the omega-3 polyunsaturated fatty acids (n-3 PUFA) in the glyceride fraction of the fish oil. In comparison with the traditional oil/water system, the enrichment factor of n-3 PUFA in this system was increased by 363.4% as a result of a higher dispersity, higher selectivity of the lipase for the other fatty acids except for n-3PUFA, and relief of product inhibition. The content of n-3 PUFA in the glyceride fraction could be concentrated to 67.97% by repeated hydrolysis after removing the free fatty acids. Furthermore, the lipase could be reused for at least eight rounds. This method would be an ideal approach for enriching n-3 PUFA because it is cost-effective, low in toxicity, and easily scaled up.

Enrichment of Short-Chain Ceramides and Free Fatty Acids in the Skin Epidermis, Liver, and Kidneys of db/db Mice, a Type 2 Diabetes Mellitus Model.

Patients with diabetes mellitus (DM) often suffer from diverse skin disorders, which might be attributable to skin barrier dysfunction. To explore the role of lipid alterations in the epidermis in DM skin disorders, we quantitated 49 lipids (34 ceramides, 14 free fatty acids (FFAs), and cholesterol) in the skin epidermis, liver, and kidneys of db/db mice, a Type 2 DM model, using UPLC-MS/MS. The expression of genes involved in lipid synthesis was also evaluated. With the full establishment of hyperglycemia at the age of 20 weeks, remarkable lipid enrichment was noted in the skin of the db/db mice, especially at the epidermis and subcutaneous fat bed. Prominent increases in the ceramides and FFAs (>3 fold) with short or medium chains (<C26) occurred in the skin epidermis (16NS, 18NS, 24NS, 16NDS, 18NDS, 20NDS, 22NDS, 24NDS, C16:1FA, C18:2FA, and C18:1FA) and the liver (16NS, 18NS, 20NS, 24:1NS, 18NDS, 20NDS, 22NDS, C16:1FA, C18:2FA, C18:1FA), whereas those with very long chains were not affected. In the kidney, only slight increases (<3 fold) were observed for 16NS, 18NS, 20NS, 26NDS, C26FA, and C22:1FA. Consistently, LXRα/β and PPARγ, nuclear receptors promoting lipid synthesis, lipid synthesis enzymes such as elongases 1, 4, and 6, and fatty acid synthase and stearoyl-CoA desaturase were highly expressed in the skin and livers of the db/db mice. Collectively, our study demonstrates an extensive alteration in the skin and systemic lipid profiles of db/db mice, which could contribute to the development of skin disorders in DM.