Enhanced Nutrient Transport Research Articles

Combining Transcriptomics and Proteomics to Screen Candidate Genes Related to Bovine Birth Weight.

We established transcriptome and proteome databases for low-birth-weight (LB) and high-birth-weight (HB) calf placentae, identifying key genes and proteins associated with birth weight through bioinformatics analyses that included functional enrichment and protein-protein interactions (PPIs). Both mRNA and protein levels were validated. A total of 1494 differentially expressed genes (DEGs) and 294 differentially expressed proteins (DEPs) were identified when comparing the LB group to the HB group. Furthermore, we identified 53 genes and proteins exhibiting significant co-expression across both transcriptomic and proteomic datasets; among these, 40 were co-upregulated, 8 co-downregulated, while 5 displayed upregulation at the protein level despite downregulation at the mRNA level. Functional enrichment analyses (GO and KEGG) and protein-protein interaction (PPI) analysis indicate that, at the transcriptional level, the primary factor contributing to differences in calf birth weight is that the placenta of the high-birth-weight (HB) group provides more nutrients to the fetus, characterized by enhanced nutrient transport (SLC2A1 and SLC2A11), energy metabolism (ACSL1, MICALL2, PAG2, COL14A1, and ELOVL5), and lipid synthesis (ELOVL5 and ELOVL7). In contrast, the placenta of the low-birth-weight (LB) group prioritizes cell proliferation (PAK1 and ITGA3) and angiogenesis. At the protein level, while the placentae from the HB group exhibit efficient energy production and lipid synthesis, they also demonstrate reduced immunity to various diseases such as systemic lupus erythematosus and bacterial dysentery. Conversely, the LB group placentae excel in regulating critical biological processes, including cell migration, proliferation, differentiation, apoptosis, and signal transduction; they also display higher disease immunity markers (COL6A1, TNC CD36, CD81, Igh-1a, and IGHG) compared to those of the HB group placentae. Co-expression analysis further suggests that increases in calf birth weight can be attributed to both high-efficiency energy production and lipid synthesis within the HB group placentae (ELOVL5, ELOVL7, and ACSL1), alongside cholesterol biosynthesis and metabolic pathways involving CYP11A1 and CYP17A1. We propose that ELOVL5, ELOVL7, ACSL1, CYP11A1, and CYP17A1 serve as potential protein biomarkers for regulating calf birth weight through the modulation of the fatty acid metabolism, lipid synthesis, and cholesterol levels.

Nanoparticles and root traits: mineral nutrition, stress tolerance and interaction with rhizosphere microbiota.

This review article highlights a broader perspective of NPs and plant-root interaction by focusing on their beneficial anddeleterious impacts on root system architecture (RSA). The root performs a vital function by securing itself in the soil, absorbing and transporting water and nutrients to facilitate plant growth and productivity. In dicots, the architecture of the root system (RSA) is markedly shaped by the development of the primary root and its branches, showcasing considerable adaptability in response to changes in the environment. For promoting agriculture and combating global food hunger, the use of nanoparticles (NPs) may be an exciting option, for which it is essential to understand the behaviour of plants under NPs exposure. The nature of NPs and their physicochemical characteristics play a significant role in the positive/negative response of roots and shoots. Root morphological features, such as root length, root mass and root development features, may regulated positively/negatively by different types of NPs. In addition, application of NPs may also enhance nutrient transport and soil fertility by the promotion of soil microorganisms including plant growth-promoting rhizobacteria (PGPRs) and also soil enzymes. Interestingly the interaction of nanomaterials (NMs) with rhizospheric bacteria can enhance plant development and soil health. However, some studies also suggested that the increased use of several types of engineered nanoparticles (ENPs) may disrupt the equilibrium of the soil-root interface and unsafe morphogenesis by causing the browning of roots and suppressing the growth of root and soil microbes. Thus, this review article has sought to compile a broader perspective of NPs and plant-root interaction by focusing on their beneficial or deleterious impacts on RSA.

Ensiled diet improved the growth performance of Tibetan sheep by regulating the rumen microbial community and rumen epithelial morphology.

The aim of this study was to investigate the effects of ensiled agricultural byproducts from Qinghai-Tibet plateau on growth performance, rumen microbiota, ruminal epithelium morphology, and nutrient transport-related gene expression in Tibetan sheep. Fourteen male Tibetan sheep were randomly assigned to one of two diets: an untreated diet (without silage inoculum, CON, n = 7) or an ensiled diet (with silage inoculum, ESD, n = 7). The total experimental period lasted for 84 d, including early 14 d as adaption period and remaining 70 d for data collection. The ESD increased average daily gain (P = 0.046), dry matter intake (P < 0.001), ammonia nitrogen (P = 0.045), microbial crude protein (P = 0.034), and total volatile fatty acids concentration (P < 0.001), and decreased ruminal pH value (P = 0.014). The proportion of propionate (P = 0.006) and the copy numbers of bacteria (P = 0.01) and protozoa (P = 0.002) were higher, while the proportion of acetate (P = 0.028) was lower in the sheep fed ESD compared to CON. Pyrosequencing of the 16S ribosomal RNA gene revealed that ESD increased the relative abundance of Firmicutes, Ruminococcus, Lachnospiraceae_AC2044_group, Lachnospiraceae_XPB1014_group, and Christensenellaceae_R-7_group in the rumen (P < 0.05), while decreased the relative abundance of Bacteroidota, Prevotellaceae_UCG-003, and Veillonellaceae_UCG-001 (P < 0.05). Analyses with PICRUSt2 and STAMP indicated that the propionate metabolism pathway was enriched in the sheep fed ESD (P = 0.026). The ESD increased the rumen papillae height (P = 0.012), density (P = 0.036), and surface area (P = 0.001), and improved the thickness of the total epithelia (P = 0.018), stratum corneum (P = 0.040), stratum granulosum (P = 0.042), and stratum spinosum and basale (P = 0.004). The relative mRNA expression of cyclin-dependent Kinase 2, CyclinA2, CyclinD2, zonula occludens-1, Occludin, monocarboxylate transporter isoform 1 (MCT1), MCT4, sodium/potassium pump, and sodium/hydrogen antiporter 3 were higher in the rumen epithelial of sheep fed ESD than CON (P < 0.05). Conversely, the relative mRNA expressions of Caspase 3 and B-cell lymphoma-2 were lower in the sheep fed ESD than CON (P < 0.05). In conclusion, compared with an untreated diet, feeding an ensiled diet altered the rumen microbial community, enhanced nutrient transport through rumen epithelium, and improved the growth performance of Tibetan sheep.

Climate Smart Irrigation Practices for Improving Water Productivity in India: A Comprehensive Review

India, supporting 17% of the global population, from limited land (2.4% of the world's total) and freshwater resources (4%), faces severe water scarcity issues. The country experiences heightened challenges due to a monsoon climate leading to floods and droughts. Irrigation efficiency in India is 35-40% and irrigated area ~48.9%. To meet the demands of a large or growing population in limited land and water resources, climate-smart irrigation practices (CSIPs) are imperative. These practices not only increase yield but also precisely supply water, reduce water application volume, and enhance soil health under changing climate conditions. Precision water management technologies includes; advanced agro-techniques, micro-irrigation, conservation agriculture, crop diversification, integrated farming systems, and water harvesting. Micro-irrigation, encompassing drip and sprinkler systems, emerges as a critical solution for efficient water use. Techniques like Surface Drip Irrigation and Sub-surface Drip Irrigation (SSDI) not only save water but also enhance nutrient transport and reduce labor costs. The automation of micro-irrigation through sensors and wireless communication revolutionizes traditional practices, ensuring precise water management and boosting agricultural productivity. In addition, advanced agro-techniques, including laser land leveling, furrow-irrigated raised beds, aerobic rice cultivation, system of rice intensification, ground cover cum rice production system and Saguna rice technique have good potential to save water and improve water productivity. Implementing these advanced agro-techniques not only conserves water but also contributes to sustainable agriculture by improving overall water productivity, reducing environmental impact, and enhancing crop productivity. The integration of conservation agriculture (minimum soil disturbance, crop residue cover and crop diversification), integrated farming systems (combine diverse agricultural activities synergistically), and water harvesting is imperative for sustainable water management. This review paper systematically compiles climate-smart irrigation practices, including precision water management, combined with conservation agriculture, crop diversification, integrated farming systems, and water harvesting. This review paper offers researchers a comprehensive understanding of different CSIPs, assessing their impact on water conservation, increased crop and water productivity, and sustainability amid climate change. Farmers can gain practical understandings of CSIPs, while policymakers obtain essential information for addressing national water mission goals.

Transcriptomic analysis reveals the molecular mechanisms of rumen wall morphological and functional development induced by different solid diet introduction in a lamb model

BackgroundThis study aimed to elucidate the molecular mechanisms of solid diet introduction initiating the cellular growth and maturation of rumen tissues and characterize the shared and unique biological processes upon different solid diet regimes.MethodsTwenty-four Hu lambs were randomly allocated to three groups fed following diets: goat milk powder only (M, n = 8), goat milk powder + alfalfa hay (MH, n = 8), and goat milk powder + concentrate starter (MC, n = 8). At 42 days of age, the lambs were slaughtered. Ruminal fluid sample was collected for analysis of concentration of volatile fatty acid (VFA) and microbial crude protein (MCP). The sample of the rumen wall from the ventral sac was collected for analysis of rumen papilla morphology and transcriptomics.ResultsCompared with the M group, MH and MC group had a higher concentration of VFA, MCP, rumen weight, and rumen papilla area. The transcriptomic results of rumen wall showed that there were 312 shared differentially expressed genes (DEGs) between in “MH vs. M” and “MC vs. M”, and 232 or 796 unique DEGs observed in “MH vs. M” or “MC vs. M”, respectively. The shared DEGs were most enriched in VFA absorption and metabolism, such as peroxisome proliferator-activated receptor (PPAR) signaling pathway, butanoate metabolism, and synthesis and degradation of ketone bodies. Additionally, a weighted gene co-expression network analysis identified M16 (2,052 genes) and M18 (579 genes) modules were positively correlated with VFA and rumen wall morphology. The M16 module was mainly related to metabolism pathway, while the M18 module was mainly associated with signaling transport. Moreover, hay specifically depressed expression of genes involved in cytokine production, immune response, and immunocyte activation, and concentrate starter mainly altered nutrient transport and metabolism, especially ion transport, amino acid, and fatty acid metabolism.ConclusionsThe energy production during VFA metabolism may drive the rumen wall development directly. The hay introduction facilitated establishment of immune function, while the concentrate starter enhanced nutrient transport and metabolism, which are important biological processes required for rumen development.

Compromised duodenal mucosal integrity in children with short bowel syndrome after adaptation to enteral autonomy

BackgroundIntestinal adaptation has been extensively studied experimentally, but very limited data is available on human subjects. In this study we assessed intestinal adaption in humans with short bowel syndrome (SBS). MethodsWe comparatively evaluated mucosal hyperplasia, inflammation, barrier function and nutrient transport using histology, immunohistochemistry and qPCR for selected 52 key genes in duodenal biopsies obtained from children with SBS after weaning off parenteral nutrition (n = 33), and matched controls without intestinal pathology (n = 12). Small bowel dilatation was assessed from contrast small bowel series. ResultsDuodenal mucosa of SBS children showed increased histologic inflammation of lamina propria (p = 0.033) and mucosal mRNA expression of tumor necrosis factor (p = 0.027), transforming growth factor (TGF)-β2 (p = 0.006) and caveolin-1 (CAV1; p = 0.001). Villus height, crypt depth, enterocyte proliferation, apoptosis and expression of proliferation and nutrient transport genes remained unchanged. Pathologic small bowel dilatation reduced crypt depth (p = 0.045) and downregulated mRNA expression of interleukin (IL)-6 by three-fold (p = 0.008), while correlating negatively with IL6 (r = -0.609, p = 0.004). Loss of ileocecal valve (ICV) upregulated mRNA expression of toll-like receptor 4 (TLR4), TGF-β1, CAV1, several apoptosis regulating genes, and mRNA expression of zonulin (p < 0.05 for all). ConclusionsDespite successful adaptation to enteral autonomy, duodenal mucosa of SBS children displayed histologic and molecular signs of abnormal inflammation and regulation of epithelial permeability, whereas no structural or molecular signs of adaptive hyperplasia or enhanced nutrient transport were observed. Excessive dilatation of the remaining small bowel paralleled impaired duodenal crypt homeostasis, while absence of ICV modified regulation of mucosal inflammation, regeneration and permeability. Level of evidenceII

The Modulatory Effect of Maternal Iron Nutriture on Placental Inflammatory Profile in a Rat Model of Prenatal Alcohol Exposure (OR26-04-19)

ObjectivesThe teratogenic effects of alcohol differ among individuals, and maternal nutriture may contribute to these differences. In this regard, our group has shown that maternal iron deficiency (ID) exacerbates alcohol’s toxicity on fetal development, and maternal iron fortification (IF) improves the poor developmental outcomes associated with prenatal alcohol exposure (PAE). Although the interactive effects of iron and PAE in fetus have been examined, their effects in placenta are less clear. Here, we sought to determine the effects of iron and PAE on placental cytokines that affect nutrient supply efficiency. We hypothesized that ID-PAE impairs placental and fetal growth, and alters placental cytokine expression to favor a pro-inflammatory profile; maternal IF attenuates some of these outcomes. MethodsWe fed pregnant rats an ID (2-6 ppm), iron-sufficient (100 ppm) or IF (500 ppm) diet throughout gestation. From gestational day (G) 13.5-19.5, we gavaged them with alcohol (5g/kg BW) or isocaloric control (maltodextrin, MD). We weighed the placentas and fetuses on G20.5, and quantified placental cytokine expression using qPCR. Data were analyzed by mixed linear model and 2-way ANOVA. ResultsAs expected, fetal weight, placental weight and placental efficiency were lower (P < 0.05) in response to PAE compared to MD. We found that PAE altered the expression of IL-1β, TNF-α and IL-10 (P = 0.034, 0.030, 0.09, respectively), but not IFN-γ, IL-6 or IL-4, suggesting a selective effect of alcohol on placental cytokine expression. Maternal ID worsened these outcomes such that ID-PAE had the lowest fetal weight and placental efficiency and a heightened pro-inflammatory placental profile. Maternal IF normalized the alcohol-reduced placental efficiency by modulating placental cytokines to enhance nutrient transport. However, it didn’t fully rescue fetal weight. Nevertheless, these cytokines were correlated with and explained 45% variability in fetal weight in this PAE model. ConclusionsThese data provide insights into how iron and alcohol interact to alter placental functions and fetal growth. Further, the cytokines reported in this study may potentially be used as diagnostic markers to identify individuals who are prenatally exposed to alcohol. Funding SourcesNIH/NIAAA F32-AA027121 (STCK), R01-AA22999 (SMS).

Fabrication of hierarchically porous silk fibroin-bioactive glass composite scaffold via indirect 3D printing: Effect of particle size on physico-mechanical properties and in vitro cellular behavior

In order to regenerate bone defects, bioactive hierarchically scaffolds play a key role due to their multilevel porous structure, high surface area, enhanced nutrient transport and diffusion. In this study, novel hierarchically porous silk fibroin (SF) and silk fibroin-bioactive glass (SF-BG) composite were fabricated with controlled architecture and interconnected structure, by combining indirect three-dimensional (3D) inkjet printing and freeze-drying methods. Further, the effect of 45S5 Bioactive glass particles of different sizes (<100 nm and 6 μm) on mechanical strength and cell behavior was investigated. The results demonstrated that the hierarchical structure in this scaffold was composed of two levels of pores in the order of 500–600 μm and 10–50 μm. The prepared SF-BG composite scaffolds utilized by nano and micro particles possessed mechanical properties with a compressive strength of 0.94 and 1.2 MPa, respectively, in dry conditions. In a wet condition, the hierarchically porous scaffolds did not exhibit any fluctuation after compression load cell and were incredibly flexible, with excellent mechanical stability. The SF-BG composite scaffold with nanoparticles presented a significant 50% increase in attachment of human bone marrow stem cells in comparison with SF and SF-BG scaffold with microparticles. Moreover, SF-BG scaffolds promoted alkaline phosphatase activity as compared to SF scaffolds without BG particles on day 14. In brief, the 3D porous silk fibroin-based composites containing BG nanoparticles with excellent mechanical properties are promising scaffolds for bone tissue regeneration in high load-bearing applications.

Mechanics of Three-Dimensional Printed Lattices for Biomedical Devices

Advances in three-dimensional (3D) printing are enabling the design and fabrication of tailored lattices with high mechanical efficiency. Here, we focus on conducting experiments to mechanically characterize lattice structures to measure properties that inform an integrated design, manufacturing, and experiment framework. Structures are configured as beam-based lattices intended for use in novel spinal cage devices for bone fusion, fabricated with polyjet printing. Polymer lattices with 50% and 70% porosity were fabricated with beam diameters of 0.4–1.0mm, with measured effective elastic moduli from 28MPa to 213MPa. Effective elastic moduli decreased with higher lattice porosity, increased with larger beam diameters, and were highest for lattices compressed perpendicular to their original build direction. Cages were designed with 50% and 70% lattice porosities and included central voids for increased nutrient transport, reinforced shells for increased stiffness, or both. Cage stiffnesses ranged from 4.1kN/mm to 9.6kN/mm with yielding after 0.36–0.48mm displacement, thus suggesting their suitability for typical spinal loads of 1.65kN. The 50% porous cage with reinforced shell and central void was particularly favorable, with an 8.4kN/mm stiffness enabling it to potentially function as a stand-alone spinal cage while retaining a large open void for enhanced nutrient transport. Findings support the future development of fully integrated design approaches for 3D printed structures, demonstrated here with a focus on experimentally investigating lattice structures for developing novel biomedical devices.

Microspongium alariae in Alaria esculenta: a widely-distributed non-parasitic brown algal endophyte that shows cell modifications within its host

Abstract Alaria esculenta is an important kelp species in northern Europe, Atlantic Canada and USA and the Arctic, with high economic potential. Microspongium alariae, a brown algal endophyte using A. esculenta as host, is reported for the first time from Scotland (Great Britain) and Brittany (France), suggesting a wide distribution in NW Europe. The alga was found growing epi-endophytically in A. esculenta stipes and was occasionally associated with warts. Isolated Microspongium thalli grew in host-free cultures and formed plurilocular sporangia in a broad range of temperature and irradiance conditions. DNA barcoding using the nuclear ribosomal ITS1, the mitochondrial COI and the plastidial rbcL confirmed the identity of the endophyte as M. alariae. Electron microscopy was used to compare the alga when endophytic in Alaria with a host-free culture. As an endophyte, cell diameter, pyrenoid diameter and cell wall thickness were reduced. In contrast, there were more plasmodesma connections between endophyte cells, possibly to enhance nutrient transport along the endophytic thallus. In the light of this evidence, a parasitic life style is considered unlikely for the species and the adaptive value of endophytism in M. alariae remains to be elucidated.

Enhanced nutrient transport improves the depth-dependent properties of tri-layered engineered cartilage constructs with zonal co-culture of chondrocytes and MSCs.

Articular cartilage is a highly organized tissue driven by zonal heterogeneity of cells, extracellular matrix proteins and fibril orientations, resulting in depth-dependent mechanical properties. Therefore, the recapitulation of the functional properties of native cartilage in a tissue engineered construct requires such a biomimetic design of the morphological organization, and this has remained a challenge in cartilage tissue engineering. This study demonstrates that a layer-by-layer fabrication scheme, including co-cultures of zone-specific articular CHs and MSCs, can reproduce the depth-dependent characteristics and mechanical properties of native cartilage while minimizing the need for large numbers of chondrocytes. In addition, introduction of a porous hollow fiber (combined with a cotton thread) enhanced nutrient transport and depth-dependent properties of the tri-layered construct. Such a tri-layered construct may provide critical advantages for focal cartilage repair. These constructs hold promise for restoring native tissue structure and function, and may be beneficial in terms of zone-to-zone integration with adjacent host tissue and providing more appropriate strain transfer after implantation.

High seeding density of human chondrocytes in agarose produces tissue-engineered cartilage approaching native mechanical and biochemical properties

Animal cells have served as highly controllable model systems for furthering cartilage tissue engineering practices in pursuit of treating osteoarthritis. Although successful strategies for animal cells must ultimately be adapted to human cells to be clinically relevant, human chondrocytes are rarely employed in such studies. In this study, we evaluated the applicability of culture techniques established for juvenile bovine and adult canine chondrocytes to human chondrocytes obtained from fresh or expired osteochondral allografts. Human chondrocytes were expanded and encapsulated in 2% agarose scaffolds measuring ∅3–4mm×2.3mm, with cell seeding densities ranging from 15 to 90×106cells/mL. Subsets of constructs were subjected to transient or sustained TGF-β treatment, or provided channels to enhance nutrient transport. Human cartilaginous constructs physically resembled native human cartilage, and reached compressive Young’s moduli of up to ~250kPa (corresponding to the low end of ranges reported for native knee cartilage), dynamic moduli of ~950kPa (0.01Hz), and contained 5.7% wet weight (%/ww) of glycosaminoglycans (≥ native levels) and 1.5%/ww collagen. We found that the initial seeding density had pronounced effects on tissue outcomes, with high cell seeding densities significantly increasing nearly all measured properties. Transient TGF-β treatment was ineffective for adult human cells, and tissue construct properties plateaued or declined beyond 28 days of culture. Finally, nutrient channels improved construct mechanical properties, presumably due to enhanced rates of mass transport. These results demonstrate that our previously established culture system can be successfully translated to human chondrocytes.

Assessing habitat exposure to eutrophication in restored wetlands: Model-supported ex-ante approach to rewetting drained mires

A multi-model-based study was performed in order to unravel valuable fen meadow habitats' possible exposure to eutrophication, which is expected to occur as a result of the re-saturation of degraded peat soils. The framework was tested in a 3000-ha fen-drain system to be restored in the Middle Biebrza Basin (northeast Poland), where the datasets and related models were used to delineate prospective eutrophication hotspots and nutrient transport. A 1-d hydrodynamic model and a 3-d groundwater flow model were applied to constitute the hydrological response of the fen-drain system to the prospective construction and function of weirs and spillways, which are expected to induce the increase of groundwater levels in degraded fens. A groundwater particle-tracking postprocessor was applied to delineate flow pathways and discharge zones and to determine water residence time in modelled layers. Soil and habitat maps, a high-resolution digital elevation model and historic groundwater level observations were applied to the model performance, calibration and spatial analysis of prospective eutrophication hotspots where increased eutrophication of groundwater can be expected due to the re-saturation of degraded peat soils. The study revealed that the large-scale fen rewetting that occurred as a result of surface water bodies' damming can potentially result in groundwater-driven nutrient dispersion along with an enhanced nutrient transport from a fen to the adjacent water bodies. Spatial analyses showed that, although the rewetting-driven eutrophication of Molinia fen meadows located in the study area is not likely, one can expect increased nutrient discharges to adjacent drains, inducing the contamination of ox-bow lakes located along the rivers. We propose the presented methodology to be applied ex-ante to fen-rewetting projects in strategic environmental assessments of restoration projects in order to manage the potentially negative environmental consequences of fen and river eutrophication with special regard to nutrient hotspots that are likely to occur within the rewetted fens.

Tubular perfusion system for chondrocyte culture and superficial zone protein expression.

Tissue engineering is an alternative method for articular cartilage repair. Mechanical stimulus has been found to be an important element to the healthy development of chondrocytes and maintenance of their native phenotype. To enhance nutrient transport and apply mechanical stress, we have developed a novel bioreactor, the tubular perfusion system (TPS), to culture chondrocytes in three-dimensional scaffolds. In our design, chondrocytes are encapsulated in alginate scaffolds and placed into a tubular growth chamber, which is perfused with media to enhance nutrient transfer and expose cells to fluid flow. Results demonstrate that TPS culture promotes the proliferation of chondrocytes compared to static culture as shown by DNA content and histochemical staining. After 14 days of culture, low messenger RNA expression of proinflammatory and apoptotic markers in TPS bioreactor culture confirmed that a flow rate of 3 mL/min does not damage the chondrocytes embedded in alginate scaffolds. Additionally, cells cultured in the TPS bioreactor showed increased gene expression levels of aggrecan, type II collagen, and superficial zone protein compared to the static group, indicative of the emergence of the superficial zone specific phenotype. Therefore, the TPS bioreactor is an effective means to enhance the proliferation and phenotype maintenance of chondrocytes in vitro.

The role of soil surface water regimes and raindrop impact on hillslope soil erosion and nutrient losses

Few investigations have addressed the interaction between soil surface water regimes and raindrop impact on nutrient losses, especially under artesian seepage condition. A simulation study was conducted to examine the effects on nitrogen and phosphorus losses. Four soil surface water regimes were designed: free drainage, saturation with rainfall, artesian seepage without rainfall, and artesian seepage with rainfall. These water regimes were subjected to two surface treatments: with and without raindrop impact through placing nylon net over soil pan. The results showed saturation and seepage with rainfall conditions induced greater soil loss and nutrient losses than free drainage condition. Nutrient concentrations in runoff from artesian seepage without rainfall condition were 7.3–228.7 times those from free drainage condition. Nutrient losses by runoff from saturation and seepage with rainfall conditions increased by factors of 1.30–9.38 and 2.81–40.11 times, and the corresponding losses with eroded sediment by 1.37–7.67 and 1.75–9.0 times, respectively, relative to those from free drainage condition. Regardless of different soil surface water regimes, raindrop impact increased 20.90–94.0 % nutrient losses with eroded sediment by promoting soil loss, but it only significantly enhanced nutrient transport to runoff under free drainage condition.

Transcriptional reprogramming by root knot and migratory nematode infection in rice

Rice is one of the most important staple crops worldwide, but its yield is compromised by different pathogens, including plant-parasitic nematodes. In this study we have characterized specific and general responses of rice (Oryza sativa) roots challenged with two endoparasitic nematodes with very different modes of action. Local transcriptional changes in rice roots upon root knot (Meloidogyne graminicola) and root rot nematode (RRN, Hirschmanniella oryzae) infection were studied at two time points (3 and 7 d after infection, dai), using mRNA-seq. Our results confirm that root knot nematodes (RKNs), which feed as sedentary endoparasites, stimulate metabolic pathways in the root, and enhance nutrient transport towards the induced root gall. The migratory RRNs, on the other hand, induce programmed cell death and oxidative stress, and obstruct the normal metabolic activity of the root. While RRN infection causes up-regulation of biotic stress-related genes early in the infection, the sedentary RKNs suppress the local defense pathways (e.g. salicylic acid and ethylene pathways). Interestingly, hormone pathways mainly involved in plant development were strongly induced (gibberellin) or repressed (cytokinin) at 3 dai. These results uncover previously unrecognized nematode-induced expression profiles related to their specific infection strategy.

Forced Convection Enhances Net Transport to the Intervertebral Disc In Vivo

The intervertebral disc (IVD) responds to repeated mechanical loading in a rate-dependent manner. Evidence indicates that high rate loading is detrimental to disc health while low rate loading may have protective effects. This may be explained by differences in nutrient transport that result from rate dependent deformation of the IVD. Low rate loading may enhance nutrient transport into the IVD via forced convection (bulk flow), whereas at higher rates there is little disc deformation and minimal bulk flow.

Decomposition of harvest residue needles on peatlands drained for forestry – Implications for nutrient and heavy metal dynamics

In the boreal and temperate zones about 15 million hectares of peatlands and wetlands have been drained for forestry purposes and a large number of these forests are now approaching their commercial thinning or regeneration age. One of the major concerns raised in connection with an increased harvesting of drained peatland forests is the deterioration of the downstream water quality due to an enhanced nutrient transport. Harvest residues left on the site are a potential high source of nutrients to recipient water bodies and both increased N and P exports have been reported after conventional stem-only clear-cutting. We studied the decomposition of Picea abies and Pinus sylvestris harvest residue needles at two clear-cut areas and two uncut forested areas on drained peatlands at two locations in southern Finland. Our results indicated that P is easily released from harvest residue needles. After the first three growing seasons, spruce and pine needles had lost approximately 31% and 47% of their initial P contents, respectively. There was no clear gain or loss of nitrogen. Most of the heavy metals accumulated in the needles as the decomposition process proceeded, especially at the more southern study location near the heavily industrialized Helsinki capital area with high atmospheric deposition. The conclusion is that harvest residue needles are not a likely source of the increased N export that has been observed to occur from peat soils soon after clear-cutting, but that P release from harvest residues may be a cause for the reported high P losses.

Tubular Perfusion System for the Long-Term Dynamic Culture of Human Mesenchymal Stem Cells

In vitro culture techniques must be improved to increase the feasibility of cell-based tissue engineering strategies. To enhance nutrient transport we have developed a novel bioreactor, the tubular perfusion system (TPS), to culture human mesenchymal stem cells (hMSCs) in three-dimensional scaffolds. This system utilizes an elegant design to create a more effective environment for cell culture. In our design, hMSCs in the TPS bioreactor are encapsulated in alginate beads that are tightly packed in a tubular growth chamber. The medium is perfused by a peristaltic pump through the growth chamber and around the tightly packed scaffolds enhancing nutrient transfer while exposing the cells to shear stress. Results demonstrate that bioreactor culture supports early osteoblastic differentiation of hMSCs as shown by alkaline phosphatase gene expression. After 14 and 28 days of culture significant increases in the gene expression levels of osteocalcin, osteopontin, and bone morphogenetic protein-2 were observed with bioreactor culture, and expression of these markers was shown to increase with media flow rate. These results demonstrate the TPS bioreactor as an effective means to culture hMSCs and provide insight to the effect of long-term shear stresses on differentiating hMSCs.

Presence of pores and hydrogel composition influence tensile properties of scaffolds fabricated from well‐defined sphere templates

Sphere templating is an attractive method to produce porous polymeric scaffolds with well-defined and uniform pore structures for applications in tissue engineering. While high porosity is desired to facilitate cell seeding and enhance nutrient transport, the incorporation of pores will impact gross mechanical properties of tissue scaffolds and will likely be dependent on pore size. The goals of this study were to evaluate the effect of pores, pore diameter, and polymer composition on gross mechanical properties of hydrogels prepared from crosslinked poly(ethylene glycol) (PEG) and poly(2-hydroxyethyl methacrylate) (pHEMA). Sphere templates were fabricated from uncrosslinked poly(methyl methacrylate) spheres sieved between 53-63 and 150-180 μm. Incorporating pores into hydrogels significantly decreased the quasi-static modulus and ultimate tensile stress, but increased the ultimate tensile strain. For pHEMA, decreases in gel crosslinking density and increases in pore diameters followed similar trends. Interestingly, the mechanical properties of porous PEG hydrogels were less sensitive to changes in pore diameter for a given polymer composition. Additionally, pore diameter was shown to affect skeletal myoblast adhesion whereby many cells cultured in porous hydrogels with smaller pores were seen spanning across multiple pores, but lined the inside of larger pores. In summary, incorporation of pores and changes in pore diameter significantly affect the gross mechanical properties, but in a manner that is dependent on gel chemistry, structure, and composition. Together, these findings will help to design better hydrogel scaffolds for applications where gross mechanical properties and porosity are critical.