<p>The primary aim of this study was to characterize the phenotypes of equines raised in the Pantanal region of Mato Grosso do Sul, one of the central-western states of Brazil. Eighteen linear measurements were collected from 498 adult horses belonging to several different breeds, including Pantaneira (n = 52), Arabic (n = 28), Crioulo (n = 31), Mangalarga (n = 8), Quarter Horse (n = 97), Petiço (n = 9), Crossbred Horses (i.e., undetermined breeding; n = 170), and Mules (n = 74). These measurements were compared to similar measurements taken of English Thoroughbreds (n = 14) and Brazilian Equestrians (n = 15) in the city of Campo Grande, the capital of Mato Grasso do Sul. We used analysis of variance to test the influence of several factors, including genetic group, category (horse or mule), sex, and their interactive effects, on phenotypic characteristics. Correlation estimates verified the Euclidean distance between genetic groups categorized via cluster analysis. The effects were significant (P &lt; 0.05) for many of the phenotypic characteristics. Horses showed higher values for most of the measurements compared to mules, except for the length of the head, lumbar back length, and cannon bone circumference, as well as greater correlation between weight and heart girth (r = 0.98). Cluster analysis determined that the animals were categorized into nine groups, with 10 distinct populations. Correspondence analysis showed that the Crioulo, Crossbred Horses, Mules, Pantaneiro, and Mangalarga breeds were less distinct phenotypically. The Petiço breed was not grouped, whereas Quarter Horses, English Thoroughbreds, and Brazilian Equestrians were more phenotypically distinct from the other breeds.</p>