In recent years, COVID-19 has occurred frequently in the world. The biosafety level 4 (BSL-4) laboratory personnel are at risk of being infected by bioaerosols. The high demand for fresh air makes the energy consumption of the laboratory far greater than that of ordinary buildings. This study aimed to propose a suitable air distribution design and reduce the energy consumption of the BSL-4 laboratory, which is beneficial for creating a healthy and green built environment. Firstly, the diffusion characteristics of aerosols, infection risk under different air distributions, and ventilation parameters were analyzed in depth. Secondly, from the perspective of negative pressure control, the influence of parameters such as air tightness and air changes on the airflow in multizone was discussed. The results indicated that the aerosol concentration under the same conditions of the upper supply-upper exhaust is 1.4 times higher than that of the upper supply-bottom exhaust after the airflow stabilizes. The impact of eddy currents on pollutant diffusion is weakened when the air supply velocity reduced from 1m/s to 0.8m/s. Finally, the energy-saving operation strategy was proposed with the optimized ventilation parameters. The results showed that this operation scheme reduced the energy consumption of the laboratory by 15-30%.
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