Food Energy Research Articles

Leveraging blockchain for sustainable supply chain management: A data privacy and security perspective

This review examines how blockchain technology can be leveraged to enhance data privacy and security in sustainable supply chain management (SSCM). As global supply chains become increasingly complex and the demand for sustainability grows, ensuring data privacy and security has become a critical concern. Traditional supply chain systems often face challenges such as data breaches, lack of transparency, and difficulty in tracing products and materials. Blockchain technology, with its decentralized, immutable, and transparent architecture, offers a promising solution to these challenges. Blockchain can enhance data security by ensuring that data is tamper-proof, traceable, and encrypted, thus protecting sensitive information across the supply chain. It provides transparency while allowing permissioned access, ensuring that stakeholders can verify data without exposing confidential information. Furthermore, privacy-preserving technologies such as zero-knowledge proofs and homomorphic encryption allow verification of data without compromising its security. Smart contracts enable automated compliance with regulatory frameworks like GDPR, reducing the risk of human error and improving operational efficiency. The integration of blockchain in SSCM can improve traceability, transparency, and accountability, thereby promoting environmental and social sustainability. By tracking the origin and journey of goods, blockchain helps verify ethical sourcing practices and reduce carbon footprints. However, the technology also presents challenges, including scalability, integration with legacy systems, and cost considerations. Through case studies in industries such as food, textiles, and renewable energy, this review highlights the practical applications and benefits of blockchain for SSCM. It concludes that blockchain has the potential to revolutionize supply chain operations, but careful consideration must be given to overcoming its technical and financial barriers to widespread adoption.

Konjac glucomannan Inhibits Appetite of Obese Mice by Suppressing Hypothalamic Inflammatory Response and Agrp/Npy Neuron Expression.

Konjac glucomannan (KGM) is used for appetite management. However, KGM's regulation of appetite through hypothalamic neurons and gut microbiota, particularly in nonobese populations, is required to be investigated. This study investigated the differential effects of KGM on appetite and energy metabolism in obese and nonobese mice. In obese mice, KGM inhibited food intake, hypothalamic inflammation, and increased energy expenditure. Conversely, in nonobese mice, KGM maintained food intake and energy expenditure but increased hypothalamic inflammation. KGM downregulated hypothalamic Agrp, Npy, and Orx expression and upregulated Cart in obese mice, while it had no effect on orexigenic genes and downregulated Cart in nonobese mice. Additionally, KGM reshaped gut microbiota and increased Short-chain fatty acids (SCFAs) formation of obese mice, where Alistipes, Bifidobacterium, and Lactobacillus, as well as SCFAs, correlated with suppressed appetite. In nonobese mice, KGM has no significant effect on SCFAs but microbes such as Blautia, Alistipes, and Flavonifractor levels were negatively correlated with hypothalamic inflammation. KGM maintains appetite and was linked to liver-derived phosphatidylcholine, countering increased hypothalamic inflammation. The differential regulation of appetite by KGM between obese and nonobese mice is associated with hypothalamic inflammatory, neuronal, and KGM-induced personalized reshaping of gut microbiota. KGM may regulate energy intake and expenditure through the microbiota-gut-brain axis.

Cholecystokinin-expressing neurons of the ventromedial hypothalamic nucleus control energy homeostasis

The hypothalamus is the primary center of the brain that regulates energy homeostasis. The ventromedial hypothalamus (VMH) plays a central role in maintaining energy balance by regulating food intake, energy expenditure, and glucose levels. However, the cellular and molecular mechanisms underlying its functions are still poorly understood. Cholecystokinin (CCK) is one of many genes expressed in this hypothalamic nucleus. Peripheral CCK regulates food intake, body weight, and glucose homeostasis. However, current research does not explain the function of CCK neurons in specific nuclei of the hypothalamus and their likely roles in network dynamics related to energy balance and food intake. This study uses genetic and pharmacological methods to examine the role of CCK-expressing neurons in the VMH (CCKVMH). Namely, using a previously generated BAC transgenic line expressing Cre recombinase under the CCK promoter, we performed targeted manipulations of CCKVMH neurons. Histological and transcriptomic database analysis revealed extensive distribution of these neurons in the VMH, with significant heterogeneity in gene expression related to energy balance, including co-expression with PACAP and somatostatin. Pharmacogenetic acute inhibition via Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) resulted in increased food intake and altered meal patterns, characterized by higher meal frequency and shorter intermeal intervals. Furthermore, diphtheria toxin-mediated ablation of CCKVMH neurons led to significant weight gain and hyperphagia over time, increasing meal size and duration. These mice also exhibited impaired glucose tolerance, indicative of disrupted glucose homeostasis. Our findings underscore the integral role of CCKVMH neurons in modulating feeding behavior, energy homeostasis, and glucose regulation. This study enhances our understanding of the neurohormonal mechanisms underlying obesity and metabolic disorders, providing potential targets for therapeutic interventions.

Freezing time, freezing in time: reconfiguring freezing practices for reducing meat waste and energy consumption

Considering that many fresh food items require domestic cold storage, understanding the role of appliances such as freezers is critical to reducing greenhouse gas emissions relating to domestic food waste and energy use. Using a social practices approach, this article reports on a study of cold storage and food waste practices, particularly freezing relating to meat, in 20 Australian households. Householders were interviewed twice, over winter and summer, 2022–2023. We studied intersecting practices of eating, shopping, cooking and cold storage, and their connection to larger social phenomena such as cold supply chains to gain insights into how and why meat was wasted. Although we set out to study household practices, we found that retailer practices of pricing specials and bulk buying must also be considered. Retailer practices combined with ambiguity in consumer understandings of date labels, shelf life and freezing times mean that domestic cold storage has become a normalised extension of the cold supply chain of food, especially for meat. We argue that understandings such as convenience and thriftiness, in conjunction with retailer expectations of domestic cold storage, may lead to the ratcheting up of household freezer use for food storage. We illustrate through our findings that this may potentially increase energy consumption and accelerate food waste, which becomes particularly problematic for carbon-intensive foods such as red meat. In innovatively reimagining domestic cold storage as an extension of industrial and retailer cold supply chains, we explore what kind of capabilities may be required for an equitable and low waste cold supply chain that reduces reliance on household freezers.

Deficiency of GPR10 and NPFFR2 receptors leads to sex-specific prediabetic syndrome and late-onset obesity in mice.

GPR10 and neuropeptide FF receptor 2 (NPFFR2) play important role in the regulation of food intake and energy homeostasis. Understanding the interaction between these receptors and their specific ligands, such as prolactin-releasing peptide, is essential for developing stable peptide analogs with potential for treating obesity. By breeding and characterizing double knockout (dKO) mice fed standard or high-fat diet (HFD), we provide insights into the metabolic regulation associated with the GPR10 and NPFFR2 deficiency. Both WT and dKO mice were subjected to behavioral tests and an oral glucose tolerance test. Moreover, dual-energy X-ray absorptiometry (DEXA) followed by indirect calorimetry were performed to characterize dKO mice. dKO mice of both sexes, when exposed to an HFD, showed reduced glucose tolerance, hyperinsulinemia, and insulin resistance compared with controls. Moreover, they displayed increased liver weight with worsened hepatic steatosis. Mice displayed significantly increased body weight, which was more pronounced in dKO males and caused by higher caloric intake on a standard diet, while dKO females displayed obesity characterized by increased white adipose tissue and enhanced hepatic lipid accumulation on an HFD. Moreover, dKO females exhibited anxiety-like behavior in the open field test. dKO mice on a standard diet had a lower respiratory quotient, with no significant changes in energy expenditure. These results provide insights into alterations associated with disrupted GPR10 and NPFFR2 signaling, contributing to the development of potential anti-obesity treatment.

Cooling Effectiveness of the Sustainable Cooling Solution for Cattle: Case Study in Poland

Recently, the dairy sector has been ever more affected by global warming. This study aimed to test a novel conductive cooling system for cattle that was successfully implemented and evaluated under summer thermally challenging weather conditions in Poland. The system consists mainly of the chiller, tank, and chilled water-driven mattress, designed to prioritize animal well-being. The experimental evaluation was carried out on three Friesian dry cows, housed on different types of bedding—commercial water mattress, straw, and cooling water mattress—and supplied with water at 10 °C (day) and 16 °C (night). The cooling water mattress’ surface temperature was twice as low as that of the commercial water mattress. The animal’s thermal comfort was assessed with physiological and behavioral reactions. The cooling effect on animals’ bodies was demonstrated with a lower reticulorumen temperature of the cooled cow (p < 0.05) than the reference ones. The local effect of cooling was proved with an 8 °C-lower skin temperature after the cow’s resting period. The presented study opens a new research direction toward dairy cattle’s welfare, sustainability, and the food–energy–water nexus, based on potential energy and water savings.

A Mix of Probiotic Strains Prevents Hepatic Steatosis, and Improves Oxidative Stress Status and Gut Microbiota Composition in Obese Mice.

The gut microbiota plays a role in fat accumulation and energy homeostasis. Therefore, probiotic supplementation may improve metabolic parameters and control body weight. In this study, mice are fed either a high-fat diet (HFD) or an HFD supplemented with oral gavage of a mixture of three probiotic strains, Bifidobacterium lactis Lafti B94, Lactobacillus plantarum HA-119, and Lactobacillus helveticus Lafti L10 for 7 weeks. It finds that probiotic supplementation modulates body weight gain, food energy efficiency, and fat accumulation caused by the HFD. This probiotic mix prevents liver damage and lipid metabolic disorders in HFD-fed obese mice. The probiotic supplementation significantly downregulates the expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and malondialdehyde (MDA) in the liver and upregulated catalase (CAT), superoxide dismutase (SOD), and nuclear respiratory factor 1 (Nrf1) expression. Mice supplemented with the probiotic mix also show different microbiota compositions, with an increase in Clostridia_UCG-014 and Lachnospiraceae_nk4a136_group and a decrease in the Dubosiella genus compared with those in mice fed only an HFD. Finally, the amounts of fecal pentanoic acid and the three bile acid species increase in mice with probiotic supplementation. Treatment with a combination of a mixture of three probiotic strains, B. lactis Lafti B94, L. plantarum HA-119, and L. helveticus Lafti L10 for 7 weeks, ameliorates the effects of HFD induced obesity in mice.

Self-Powered Flexible Sensors: From Fundamental Mechanisms toward Diverse Applications

Abstract Today, human life is inseparable from energy for clothing, food, housing, use and travelling, however, traditional resources can no longer meet our energy needs today. Self-powered sensing devices that can run consistently and sustainably without a source of power from the outside are top choices for new energy sources with their unique advantages. Based on advances in the field of materials and manufacturing techniques, it is able to autonomously extract energy from different sources. In this review, we focus on reviewing the current state of research on self-powered wearable sensors, providing a concise overview of energy harvesting technologies, energy conversion mechanisms, structural or material innovations, energy storage and processing platforms. Then，experimental advances in different energy sources are presented, showing their underlying mechanisms, and the potential for energy acquisition. In addition, we discuss applications of self-powered flexible sensors in medicine, sports and food. Despite considerable achievements in this field, widespread commercialization will necessitate enhanced sensor detection abilities, improved design factors for adaptable devices, and a harmony between sensitivity and standardisation.

People of the Sea, or of the Soil? How the Balance of Marine and Terrestrial Resource Availability Informs Maximum Population on Four Polynesian Islands

AbstractMost studies of the food resource potential of early Polynesian populations focus exclusively on agricultural potential, and specifically starchy staples, despite the importance of marine resources to the Polynesians. To more accurately estimate total precontact food resource availability, we characterized the terrestrial and near-shore marine environments of four Polynesian islands: Moʻorea, Maupiti, Mangareva and Taravai. We estimate the agricultural potential of each island after a consideration of the ecological factors related to productivity. We also estimate the productivity of the near-shore marine environment as a function of surface area. Using a range of fish yield scenarios from Pacific subsistence systems we scale relative measures of faunal food biomass derived from local archaeological excavations to generate absolute biomass estimates. We convert our estimates of agricultural and fished/foraged food potential into a maximum population size based on calorie availability. Moʻorea’s fertile valleys and wetlands would potentially generate sufficient food energy, mostly from starchy staples, to support a considerable population, many times larger than the other islands. In the most likely fish yield scenario Moʻorea gets only 0.4% of its calories from marine sources, while the others range from 7–18%. These relative inputs reflect the vast superiority in productivity (in terms of calories per km2) of agricultural vs near-shore marine zones. We also find that population size on islands with smaller fringing reefs, such as Moʻorea, may have been limited by a lack of fish protein. Moʻorea’s maximum population is approximately halved when diet breadth is considered.

8369 Overexpression of Neurosecretory Protein GL, a Novel Small Secretory Protein, Induces Fat Accumulation in Mice

Abstract Disclosure: K. Ukena: None. Y. Narimatsu: None. A. Ogasawara: None. M. Furumitsu: None. E. Iwakoshi-Ukena: None. Hypothalamic neuropeptides and peripheral factors influence food intake, body mass, and energy expenditure. For instance, neuropeptide Y (NPY)/agouti-related peptide (AgRP) are potent orexigenic factors and α-melanocyte-stimulating hormone (α-MSH) is an anorexigenic factor, respectively. In addition, leptin and insulin derived from peripheral tissues act on the hypothalamus to influence energy homeostasis. Precise hypothalamic control of energy balance affects puberty, thermoregulation, energy storage, and survival during different life-history stages. We have previously discovered a novel cDNA encoding the precursor of a neurosecretory small protein in the hypothalamus of birds and rodents (1, 2). The precursor protein contained a signal peptide sequence, a mature peptide sequence with 80 amino acid residues, a glycine amidation signal, and a dibasic amino acid cleavage site. Because the predicted C-terminal amino acids of the peptide were Gly-Leu-NH2, the small protein was named neurosecretory protein GL (NPGL). Quantitative RT-PCR analysis demonstrated that NPGL mRNA was expressed exclusively in the mediobasal hypothalamus (MBH), including the arcuate nucleus, and the expression was elevated on food deprivation in rodents. This finding led us to predict that NPGL was involved in the regulation of energy homeostasis. Indeed, using NPGL administration and neutralizing antibody administration in rats, we established that NPGL increased lipid accumulation in white adipose tissue (3). In the present study, to investigate the physiological function of NPGL in mice, we induced its overexpression using an adeno-associated virus (AAV) vector in NPGL-Cre driver mice. We then monitored food intake and recorded body mass changes. Npgl overexpression increased body mass. We also found that the overexpression resulted in increased masses of white adipose tissue and interscapular brown adipose tissue. Taken together, these results provide evidence for NPGL as a novel regulator of fat deposition in adipose tissues. Reference: (1) Ukena et al., Biochem. Biophys. Res. Commun. 2014 446:298-303. (2) Ukena, Gen. Comp. Endocrinol. 2018 260:164-170. (3) Iwakoshi-Ukena et al., eLife 2017 6:e28527. Presentation: 6/3/2024

Assessing the Role of Nutrition Knowledge in Shaping Dietary Practices Among Secondary Level School Students: Implications for Healthcare Management

Introduction: This study investigates the nutrition knowledge and dietary practices among secondary-level school students, a demographic prone to unhealthy habits such as junk food consumption, meal skipping for weight loss, and non-compliance with balanced diets. These behaviors increase the risk of non-communicable diseases like obesity and diabetes in adolescents. This study has aims to examine the nutrition knowledge and dietary practices of secondary-level school students, a demographic that is increasingly susceptible to unhealthy eating habits. Specifically, it focuses on behaviors such as junk food consumption, meal skipping for weight loss, and the neglect of balanced diets. These specific practices contribute to a heightened risk of non-communicable diseases, including obesity and diabetes, among adolescents. Methodology: The quantitative research approach was employed an exploratory design, analyzing data from 121 students in Kathmandu valley using a close-ended questionnaire during 7th April to 28th May 2024. The systematic sampling technique was used, and respondents were selected by using a lottery system. As a result, Grade 11 was picked up randomly. Descriptive analyses were made to describe the practices of the students. Result (s): Study indicates that out of 121 respondents, about near to half (48.8%) were male and little more than half (51.2%) were female. Similarly, there were no significant relationship exists between nutrition knowledge and dietary practices among the students. Likewise,, independent variables such as age, gender, and religion do not significantly influence either nutrition knowledge or dietary practices. Despite the students' understanding of nutrition, this knowledge does not translate into healthier eating behaviors. Conclusion and recommendation: The study reveals that there are notable gaps in their knowledge, particularly regarding the energy content of fat-free foods, the role of dietary fiber, and the impact of empty-calorie foods and meal-skipping on health. Likewise, findings highlight the disconnect between what students know about nutrition and how they apply it in their daily lives, suggesting the need for more effective interventions to encourage healthier dietary practices. Moreover, targeted nutritional education programs at school could address these gaps in knowledge and encourage healthier dietary practices.

World Futures Day 2023 Global futures agenda by The Millennium Project

World Futures Day (WFD), the 24 h round-the-world participatory method hosted every year by The Millennium Project and others on March 1, stimulates conversations and collective intelligence on possible futures. The 2023 edition marked its tenth anniversary. The 2023 Global futures agenda coming out from the data analysis is composed of four main clusters, each listing future oriented topics and related contents. Cluster 1, Complex solutions for complex problems, includes topics such as Environmental regeneration, Predictable food systems, Energy transition, Homo Galaxia, and Synergetic relations for peace. Cluster 2, A hyper-technological humanity, includes topics such as A biological revolution, New definitions of truth, An emotional AGI, A conscious AGI, and AGI governance. Cluster 3, Education and learning for a better future, includes topics such as Intelligent agent teachers, Intergenerational storytelling, and A cultural shift to self-actualization. Cluster 4, Improving global foresight, includes topics such as Futurists as a contradiction, A new leadership agenda, Futures shapers, The power of stories, Judging trillions of scenarios, Committees and summits for the future. After discussing some changes in the contents addressed over the years and some potential next steps for the research on WFD, the conclusions report that one key takeaway from WFD 2023 is that we have a vital need for pragmatic hope.

Review: Urban Water Security and Safety

“Water security” and “water safety” is defined differently. As the terms are related they may lead to confusion and misinterpretations, depending on the context. Water security generally refers to a lack of resources of an acceptable quality, i.e. water scarcity that may be related either to an excess of water demand or drought impacts (with links to climate change and e.g. salt water intrusion into coastal aquifers). Further, water security is closely related to food security, energy security, health security and ecological security. From a (human) security viewpoint, however, water security may also be understood in the light of possible intentional degradation of the resources, e.g. criminal or terrorist act leading to a deliberate (chemical or biological) contamination of water supply systems. Water safety on the other hand refers to the quality or chemical status of the water resources that has to comply with the defined quality standards for drinking water specifically to protect human health, both from elevated concentrations of contaminants and natural geogenic elements. This review gives a snapshot of various (ground)water safety and security issues written by authors from different sectors and disciplines. Illustrating and clarifying the many societal challenges related to water security and safety in cities.

The Impact on People who Trade on the Fish Species ‘Limnothrissa miodon’ - Kapenta for their Livelihood: Practical Perceptions of Traders of Siavonga

Lake Kariba is the world’s largest man-made lake and reservoir by volume. It lies 1300km upstream from the Indian Ocean, along the border between Zambia and Zimbabwe. Lake Kariba was filled between 1958 and 1963 following the completion of the Kariba dam at its north eastern end flooding the Kariba Gorge on the Zambezi River. Culturally controversial, the local people believed that the construction of the dam would anger the Zambezi river god Nyami-nyami, who rise up and cause the dam would wall to collapse destroying the bridge as well as resulting in floods of catastrophic proportions. It is believed that the Nyami-nyami lives under a rock close to the Kariba dam wall. The rock is called ‘Kariwa’ meaning trap as the local people believed if you canoed past its location you would be sucked down in whirlpool never to be seen again. This where the name Kariba originates from, laced with the local mythology. In the late 1960s a type of fish Limnothrissa miodon locally known as Kapenta was introduced in Lake Kariba from lake Tanganyika. Kapenta is a small sardine fish which supports a large and viable fishery for Zimbabwe and Zambia. Kapenta, a type of fish also known as fresh water sardine has several chemical compositions that help reduce the risk of various diseases including prostate cancer. A recent chemical composition analysis of Kapenta by the National Food and Nutrition Commission (NFNC) shows a great variation in the nutrient composition for the dry and fresh Kapenta. NFNC principal nutritionist Mr. Musonda Mofu says dry Kapenta contains 209 calories of food energy compared with 85 calories of food energy in fresh Kapenta. In addition dry Kapenta contains 63 grammes of protein and only 16 grams of protein in fresh Kapenta. The paper reflects on the declining population of this small nutrient rich fish due to over-harvesting, climatic changes with increasing temperatures and with the decreasing levels of water storage. A cumulative study on the pattern of nurturing the fish for economic and social flexibility is the main concern of this paper.

Examining Trends in the Food–Energy–Water Security Nexus and Its Relationships with Human Development, Population Growth, and Conflict

Abundant and accessible food, energy, and water are essential to the functioning of human societies and individual health and happiness. However, growing populations, intensifying climate change, and violent instability undermine resource security. We used data from the recently updated Pardee RAND Food–Energy–Water (FEW) Index and the INFORM risk model to assess global and regional trends in resource security and relationships with the Human Development Index (HDI), internal conflict risk, and population growth. Our analysis finds that resource security has modestly improved with an average global FEW Index improvement of 2.61%, but this figure masks significant regional disparities, with several countries in Sub-Saharan Africa and South Asia regressing in terms of food and water security. We observe continued high correlations between HDI and the FEW Index (0.8664) and also find the FEW Index to be highly correlated with national income per capita (0.6547). We also find that at a global level, there is a significant negative association between trends in the FEW Index and population growth (−0.4724) during the study period, suggesting that a growing proportion of the global population is experiencing resource insecurity. Finally, our analysis suggests that several resource-insecure countries are also conflict-prone and these nations tended to do worse over the study period than similarly resource-insecure states that were internally stable.

Higher sweet beverage consumption was associated with increased gestational weight gain and birth weight: A Chinese cohort study

Diet during pregnancy is crucial to maternal metabolism and fetal development, so exploring the most potent food risk factor could improve maternal and child health. In this study, we investigated the diet and lifestyle of 833 healthy pregnant women in the second trimester from November 2020 to August 2021. Based on the Tianjin Antenatal Care System in China, we followed up with these women and recorded their gestational weight gain (GWG) and newborn birth weight. We conducted a dietary survey through FFQ based on the food groups recommended by the Chinese Dietary Guidelines and included common ultra-processed foods. We collected 219 semi-quantitative FFQs and 614 self-reported FFQs for analysis. According to the consumption frequency of 12 food groups, 4 dietary patterns were extracted by principal component analysis. We analyzed the associations of food energy, consumption frequency, and dietary patterns with GWG and birth weight, especially GWG in the first and second trimesters (f-GWG). The results showed that f-GWG was positively correlated with food energy. Beverage consumption was associated with f-GWG (r = 0.288, P = .026) in obese pregnant women. A dietary pattern that favors high consumption of ultra-processed foods (fried foods, baked desserts, and sweet beverages) was associated with increased GWGs. Non-obesity women with high consumption of baked desserts and sweet beverages had higher GWGs (P < .05). After adjusting for confounding factors (including total energy, physical activity, and sleep quality), only sweet beverage consumption was associated with f-GWG (β 0.498, 95%CI 0.153-0.843) and birth weight (β 0.124, 95%CI 0.009-0.240). Sweet beverage consumption is a key adjustable risk factor for prenatal care.

Thyroid Hormone Clearance in the Paraventricular Nucleus of Male Mice Regulates Lean Mass and Physical Activity.

The actions of thyroid hormones (THs) in the central nervous system are relevant to food intake and energy expenditure. TH receptors exhibit high expression in brain areas modulating energy balance, including the arcuate, paraventricular (PVN), supraoptic, and ventromedial (VMH) hypothalamic nuclei. To examine the role of THs in the regulation of energy balance via action in specific hypothalamic nuclei of the adult mouse, we performed experiments of conditional inactivation of DIO3, the enzyme responsible for the clearance of THs, in the lateral hypothalamus (LH), and VMH and PVN hypothalamic nuclei. We accomplished DIO3 genetic inactivation via stereotaxic injection of the AAV-cre vector into adult mice homozygous for a "floxed" Dio3 allele. Dio3 inactivation in the LH and VMH of males or females did not result in significant changes in body weight 8 weeks after injection. However, inactivation of Dio3 in the PVN resulted in increased body weight (both fat mass and lean mass) and locomotor activity, and decreased hypothalamic Mc4r expression in male, but not female mice. However, PNV-specific Dio3 KO did not cause hyperphagia. These results suggest local TH action influences MC4R signaling and possibly other PVN-associated circuitries, with consequences for body composition and energy balance endpoints, but not for orexigenic pathways. They also support a regulatory role for PVN Dio3 in the central regulation of energy homeostasis in adult life.

Increased cancellous bone mass accompanies decreased cortical bone mineral density and higher axial deformation in femurs of leptin-deficient obese mice

IntroductionLeptin is a pleiotropic hormone that regulates food intake and energy homeostasis with enigmatic effects on bone development. It is unclear if leptin promotes or inhibits bone growth. The aim of this study was to characterize the micro-architecture and mechanical competence of femur bones of leptin-deficient mice. Materials and methodsRight femur bones of 15-week old C57BL/6 (n = 9) and leptin-deficient (ob/ob, n = 9) mice were analyzed. Whole bones were scanned using micro-CT and morphometric parameters of the cortex and trabeculae were assessed. Elastic moduli were determined from microindentations in midshaft cross-sections. Mineral densities were determined using quantitative backscatter scanning electron microscopy. 3D models of the distal femur metaphysis, cleared from trabecular bone, were meshed and used for finite element simulations of axial loading to identify straining differences between ob/ob and C57BL/6 controls. ResultsCompared with C57BL/6 controls, ob/ob mice had significantly shorter bones. ob/ob mice showed significantly increased cancellous bone volume and trabecular thickness. qBEI quantified a ∼7% lower mineral density in ob/ob mice in the distal femur metaphysis. Indentation demonstrated a significantly reduced Young's modulus of 12.14 [9.67, 16.56 IQR] GPa for ob/ob mice compared to 23.12 [20.70, 26.57 IQR] GPa in C57BL/6 mice. FEA revealed greater deformation of cortical bone in ob/ob as compared to C57BL/6 mice. ConclusionLeptin deficient ob/ob mice have a softer cortical bone in the distal femur metaphysis but an excessive amount of cancellous bone, possibly as a response to increased deformation of the bones during axial loading. Both FEA and direct X-ray and electron microscopy imaging suggest that the morphology and micro-architecture of ob/ob mice have inferior biomechanical properties suggestive of a reduced mechanical competence.

Do calorie labels change energy purchased in a simulated online food delivery platform? A multi-arm randomised controlled trial

BackgroundAs rates of obesity and overweight continue to increase in the UK, calorie labels have been introduced on menus as a policy option to provide information to consumers on the energy content of foods and to enable informed choices. This study tested whether the addition of calorie labels to items in a simulated food delivery platform may reduce the energy content of items selected.MethodsUK adults (n = 8,780) who used food delivery platforms were asked to use the simulated platform as they would in real life to order a meal for themselves. Participants were randomly allocated to a control condition (no calorie labels) or to one of seven intervention groups: (1) large size calorie labels adjacent to the price (LP), (2) large size label adjacent to the product name (LN), (3) small label adjacent to price (SP), (4) small label adjacent to product name (SN), (5) LP with a calorie label switch-off filter (LP + Off), (6) LP with a switch-on filter (LP + On), or, (7) LP with a summary label of the total basket energy content (LP + Sum). Regression analysis assessed the impact of calorie labels on energy content of foods selected compared to the control condition.ResultsThe mean energy selected in the control condition was 1408 kcal (95%CI: 93, 2719). There was a statistically significant reduction in mean energy selected in five of the seven intervention trial arms (LN labels (-60 kcal, 95%CI: -111, -6), SN (-73, 95%CI: -125, -19), LP + Off (-110, 95%CI: -161, -57), LP + On (-109, 95%CI: -159, -57), LP + Sum (-85 kcal, 95%CI: -137, -30). There was no evidence the other two conditions (LP (-33, 95%CI: -88, 24) and SP (-52, 95%CI: -105, 2)) differed from control. There was no evidence of an effect of any intervention when the analysis was restricted to participants who were overweight or obese.ConclusionAdding calorie labels to food items in a simulated online food delivery platform reduced the energy content of foods selected in five out of seven labelling scenarios. This study provides useful information to inform the implementation of these labels in a food delivery platform context.

SIK2 Controls the Homeostatic Character of the POMC Secretome Acutely in Response to Pharmacological ER Stress Induction.

The neuronal etiology of obesity is centered around a diet-induced inflammatory state in the arcuate nucleus of the hypothalamus, which impairs the functionality of pro-opiomelanocortine neurons (POMCs) responsible for whole-body energy homeostasis and feeding behavior. Intriguingly, systemic salt inducible kinase 2 (SIK2) knockout mice demonstrated reduced food intake and energy expenditure along with modestly dysregulated metabolic parameters, suggesting a causal link between the absence of SIK2 activity in POMCs and the observed phenotype. To test this hypothesis, we conducted a comparative secretomics study from POMC neurons following pharmacologically induced endoplasmic reticulum (ER) stress induction, a hallmark of metabolic inflammation and POMC dysregulation in diet-induced obese (DIO) mice. Our data provide significant in vitro evidence for the POMC-specific SIK2 activity in controlling energy metabolism and feeding in DIO mice by regulating the nature of the related POMC secretome. Our data also suggest that under physiological stress conditions, SIK2 may act as a gatekeeper for the secreted inflammatory factors and signaling molecules critical for cellular survival and energy homeostasis. On the other hand, in the absence of SIK2, the gate opens, leading to a surge of inflammatory cytokines and apoptotic cues concomitant with the dysregulation of POMC neurons.