Biomechanical energy harvesting from elastically-suspended load carriage is a promising source of power for Soldiers, who often march with heavy loads at varying speeds on various terrains. An energy harvesting backpack (EHB) has been developed which generates power from vertical oscillations during locomotion. Ideally, the EHB should not increase psycho-physiological burden compared to the standard military assault pack (AP). PURPOSE: To compare ratings of perceived exertion (RPE) while walking with an EHB and an AP at different speeds on different grades. METHODS: 16 subjects (M±SD; 28.6±4.9 years; 173.4±10.6 cm; 78.7±16.4 kg) walked on a treadmill with each pack for 5 minutes at each of three grades (+5%, 0%, and -5%) and each of two speeds (1.34 m/s and self-selected faster speed). Both the AP and EHB contained a 15.9 kg load, but the design of the EHB made it 4.4 kg heavier than the AP. A Borg RPE score was taken during the last 10 seconds of walking at each grade and speed. A within-subjects ANOVA was used to determine effects of pack, grade, and speed on log-transformed RPE. Alpha level was set a priori at p<0.05. Post-hoc comparisons were explored using Bonferroni corrections. RESULTS: There were main effects for pack, speed, and grade (F1,152=14.3, F1,152=100.1, and F1,152=346.3, respectively; p<0.001) with no interaction effects. Subjects reported a greater sense of exertion with the EHB (11.9±2.8) than with the AP (11.2±2.6) regardless of speed and grade. Faster speeds elicited higher RPE scores than slower speeds (13.1±2.4 and 10.1±2.1, respectively), while the incline grade produced higher RPE scores (13.1±2.5) than decline and level grades (10.3±2.3 and 11.2±2.5, respectively). CONCLUSIONS: The EHB caused greater levels of perceived exertion that were not altered by walking speed or grade. This may be due to the extra stabilization required or the extra weight cost of the EHB. Kinematic variables (trunk lean) related to this research suggest potential non-linear effects of EHB use, which may also be related to the increased perceptions of exertion found here. This may affect trade-offs between power generation, perceived exertion, and metabolic cost that warrants further research and may ultimately affect user-acceptance of suspended-load energy harvesting systems in the field.