Energy Drinks Research Articles

Assessing the Impact of Pomegranate Juice on Liver Tissue in Male Rats Exposed to a Commercial Energy Drink

The current study was designed to investigate the effect of pomegranate juice on some biochemical and histological variables resulting from dosing male rats with the energy drink Red Bull. In this study, 28 white male rats were used, divided into four groups, each involving seven animals, according to the following: G1 was given a physiological solution (5 ml/kg) and a standard diet for 120 days; G2 was given a dose of the energy drink Red Bull (volume 1 ml per 100 g); G3 was given pomegranate juice (5 ml/kg) first; then Red Bull; and G4 was given Red Bull first; and after six weeks, he was given pomegranate juice. The results showed that Red Bull consumption significantly elevated RBS, AST, ALT, TC, and TG levels. Pomegranate juice has the ability to reduce the concentration of RBS, AST, ALT, TC, and TG. Histological changes in the liver of animals that were given the Red Bull energy drink were represented by congestion of the central vein, degeneration, and necrosis of liver cells, with severe infiltration of inflammatory cells and a thick wall of the central vein (TW), as well as amyloid (AM) deposition, compared to the negative control group. The results of the histological examination showed that pomegranate juice could repair cellular tissue in the liver and make it similar to the tissue of a control normal group, denoting the possibility of using it in the diet and in treating atherosclerosis and heart disease. Keywords: Pomegranate juice, liver, rats, energy drink, rat model, atherosclerosis, heart disease.

A Green Liquid Chromatography Method for Simultaneous Quantification of Caffeine and its Three Major Metabolites in Urine, Drinks, and Herbal Products

ABSTRACTA green reversed‐phase liquid chromatography method was developed to determine caffeine (CF), theobromine (TB), theophylline (TP), and paraxanthine (PX). The stationary phase was a silica‐based C18 monolithic column (100 × 3.0 mm2 i.d.), eluted with a gradient from 6% to 10% (v/v) of ethanol in water, at a flow rate of 0.75 mL/min (35°C) and UV detection at 272 nm. The compounds were separated in less than 6 min, with a total time of 10 min for column reconditioning. The effect of gradient composition, flow rate, injection volume, and column temperature was evaluated in the separation efficiency, especially regarding the critical pair PX/TP, for which the best resolution was 1.37. The linear range was between 0.5 and 100 µg/mL. The LODs were 0.065, 0.057, 0.040, and 0.044 mg/mL for TP, TB, PX, and CF, respectively. The method was applied to green tea and yerba mate infusions, soft drinks, energy drinks, and soluble coffee. Urine samples were analyzed before and after solid‐phase extraction. The accuracy was evaluated by the spiking/recovery strategy standing in the 79.6% to 116.4% range. The separation performance and the mobile phase consumption were compared to those of another method using a gradient of methanol in water.

A Path Model of Factors Associated with Hypertension and Disease: Analysis of Indonesian Basic Health Survey Year 2018

Non-communicable diseases are estimated to account for 73% of all deaths in Indonesia, and cardiovascular disease contributes 35%. Unhealthy dietary behavior leads to several NCDs, such as Diabetes Mellitus, obesity, hypertension, cardiovascular disease, and stroke. This cross-sectional study used secondary data from the Indonesian Basic Health Survey Year 2018. The study selected 10,171 samples aged ≥15 years from West Borneo Province. The dependent variable was coronary disease. The independent variables were hypertension, age, gender, education, smoking, vegetables, fruit consumption, fat intake, alcohol consumption, instant noodles consumption, soft drink consumption, physical activity, and residence. A path analysis was conducted data analysis. The coronary disease was directly increased by hypertension (b=1.19; 95% CI=0.90 to 1.48; p 0.001), age ≥43 years (b=0.88; 95% CI=0.55 to 1.21; p 0.001), and high physical activity (b= -0.49; 95% CI= -0.81 to -0.17; p=0.003). It was directly decreased by alcohol consumption, but it was statistically non-significant (b=-0.71; 95% CI=-1.54 to 0.11; p=0.088). Coronary disease was indirectly affected by males, smoking, vegetable consumption, fruit consumption, fat intake, grilled food consumption, energy drink consumption, instant noodles consumption, soft drink consumption, residence, and education.

The possible protective effect of querectin and omega 3 on cardiac muscle changes caused by energy drinks in adult male albino rats: Histological and immunohistochemical study

The possible protective effect of querectin and omega 3 on cardiac muscle changes caused by energy drinks in adult male albino rats: Histological and immunohistochemical study

Anisaldehyde-based deep eutectic solvent dispersive liquid–liquid microextraction (DES-DLLME) followed by UPLC–MS/MS for simultaneous determination of selected parabens and bisphenols in food products: Method development and assessment of the ecological implications

A rapid and environmentally friendly method based on dispersive liquid–liquid microextraction (DLLME) technique, coupled with UPLC–MS/MS analysis was employed for simultaneous determination of selected bisphenols and parabens residues in food products. In this research, anisaldehyde and decanoic acid with a molar ration of 3:1 was prepared as a natural deep eutectic extracting solvent. Various extraction parameters such as the volume of the natural deep eutectic solvent, vortex duration, centrifugation duration, sample volume and pH were systematically explored and optimized. Determination coefficients of ≥0.9990 were achieved for the analyzed compounds under the optimal conditions. The limits detection were in the range of 0.0005 to 0.02 µg/L, while extraction recoveries ranged from 97.8 % to 100.2 % with relative standard deviations (%RSD) not exceeding 6.8 %. To assess the practical applicability of the proposed method, it was employed for the determination of five parabens and three bisphenols in eighty food products including soft drinks, energy drinks, sauces and juices collected from the Saudi market. The environmental implications of the proposed method were assessed using the following evaluation tools, Eco-Scale Assessment (ESA), Green Analytical Procedure Index (GAPI) and Analytical GREEnness metric (AGREE). To the best of our knowledge, this is the first study to determine the investigated analytes using anisaldehyde based deep eutectic solvent.

Research on the level of awareness and consumption of energy drinks among students aged 18-25

Introduction and purpose: The article focuses on the analysis of young people's awareness of the consumption of energy drinks, especially those containing caffeine. The aim of the study is to understand the level of knowledge about the awareness and consumption of energy drinks among students aged 18-25. This study was conducted using surveys and involving 202 participants. Material and Methods: The study was conducted using online anonymous surveys, involving 202 participants. The participants were students aged 18-25 both women and men. The results were analyzed according to the age and gender. Moreover, based on the available literature examining aspects related to the consumption of energy drinks.Results: The study shows that the vast majority of respondents (94.06%) are aware of the impact of energy substances on the body. The charts also illustrate that caffeine, as the main ingredient in energy drinks, is well understood in terms of cardiovascular effects. Moreover, the student community is aware of the link between excessive sugar consumption and the risk of cancer development. Discussion: The study shows that young people are aware of the negative impact of energy drinks on health. Nevertheless, not all areas of knowledge are clear to them. The continuous increase in the level of education can undoubtedly contribute to the introduction of healthy eating habits among students. Most respondents declare to drink energy drinks again, it should draw our special attention.Conclusions: It is worth noting the need for further education, especially in the field of metabolic syndrome. It also highlights the role of research and education in shaping public health awareness. Finally, the article suggests that further research and targeted educational programs are critical to improving public health, especially in the context of energy drink consumption.

Toxicological impact of energy drinks on the heart and liver tissues of nursing female rats and their neonates

Toxicological impact of energy drinks on the heart and liver tissues of nursing female rats and their neonates

Analysis of Caffeine Content in Energy Drink and Its Impact on Students Health (by using UV-Visible Spectroscopy, HPTLC and Microscopy)

Analysis of Caffeine Content in Energy Drink and Its Impact on Students Health (by using UV-Visible Spectroscopy, HPTLC and Microscopy)

Sudden cardiac arrest occurring in temporal proximity to consumption of energy drinks

BackgroundEnergy drinks potentially can trigger life-threatening cardiac arrhythmias. It has been postulated that the highly stimulating and unregulated ingredients alter heart rate, blood pressure, cardiac contractility, and cardiac repolarization in a potentially proarrhythmic manner. ObjectiveThe purpose of this study was to describe our experience regarding sudden cardiac arrest (SCA) occurring in proximity to energy drink consumption in patients with underlying genetic heart diseases. MethodsThe electronic medical records of all SCA survivors with proven arrhythmias referred to the Mayo Clinic Windland Smith Rice Genetic Heart Rhythm Clinic for evaluation were reviewed to identify those who consumed an energy drink before their event. Patient demographics, clinical characteristics, documented energy drink consumption, and temporal relationship of energy drink consumption to SCA were obtained. ResultsAmong 144 SCA survivors, 7 (5%; 6 female; mean age at SCA 29 ± 8 years) experienced an unexplained SCA associated temporally with energy drink consumption. Of these individuals, 2 had long QT syndrome and 2 had catecholaminergic polymorphic ventricular tachycardia; the remaining 3 were diagnosed with idiopathic ventricular fibrillation. Three patients (43%) consumed energy drinks regularly. Six patients (86%) required a rescue shock, and 1 (14%) was resuscitated manually. All SCA survivors have quit consuming energy drinks and have been event-free since. ConclusionOverall, 5% of SCA survivors experienced SCA in proximity to consuming an energy drink. Although larger cohort studies are needed to elucidate the incidence/prevalence and quantify its precise risk, it seems prudent to sound an early warning on this potential risk.

Consumption Rate of Energy Drinks among Kabul University Students: Caffeine Contents and Microbiological Examinations of their Available Varieties at Kabul City

Energy drinks (EDs) have gained popularity as a beverage all around the world, but as their usage has grown, questions have been raised concerning their high caffeine concentration and potential for microbial contamination. The objective of this study was to evaluate EDs. consumption rate among Kabul University students, the determination of caffeine contents, microbiological quality, and pH of EDs available in Kabul City. 184 Kabul University students were selected using simple random selection to assess the types, amounts of consumption, and awareness about the side effects of EDs. The caffeine levels in EDs were quantified using a UV/Vis spectrophotometer. The EDs were cultured in various culture media for microbiological studies. 23 of the 184 students were regular users of EDs. The mean concentration of caffeine was 277.43 ppm with an SD of 137.49 to 143.10. The EDs had no microbiological contamination, and their pH ranged from 2.64 to 3.64. 12.5% of the students regularly used EDs, Caffeine levels in all of the evaluated EDs were below recommended levels, and none of them contained any bacteria contamination.

ФАКТОРЫ РИСКА РЕЗИСТЕНТНОСТИ ЮНОШЕСКОЙ МИОКЛОНИЧЕСКОЙ ЭПИЛЕПСИИ

Abstract. Introduction. Juvenile myoclonic epilepsy is the most common form of genetic generalized epilepsy, which is included in the group of idiopathic generalized epilepsies. The etiology of drug-resistant forms of juvenile myoclonic epilepsy, which represent a serious clinical problem, is currently a controversial issue. Aim. To reveal the most significant risk factors of resistance in juvenile myoclonic epilepsy. Materials and Methods. The observational retrospective study included 56 patients, 46 (82%) women and 10 (18%) men. Inclusion criteria: patients diagnosed with juvenile myoclonic epilepsy based on the criteria of the International League Against Epilepsy, treatment for at least 2 years. The type and frequency of epileptic seizures, the onset and course of the disease, concomitant diseases, family history, all available results of 1-2 hours video electroencephalographic monitoring with sleep, and magnetic resonance imaging results were analyzed. Depending on the treatment effect, all patients were divided into two groups: 1 – without seizures, 2 – with seizures. Lack of complete seizure control for at least 2 years was considered intractable epilepsy. Risk ratios as outcomes for dichotomous variables in two compared groups for various characteristics and their confidence intervals were calculated using the Review Manager program (v5.3). Differences were considered significant at P<0.05. Results and Discussion. As a result, there were obtained data on the resistance of juvenile myoclonic epilepsy depending on the age of disease onset, the type of epileptic seizures, changes in the electroencephalogram, and the type of antiepileptic treatment. Conclusions. The most significant risk factors of resistance in juvenile myoclonic epilepsy have been identified: frequent generalized tonic-clonic seizures (more than 5 times a year) accompanying the basic disease, depression, anxiety, and lack of response to the treatment with valproic acid. At the same time, non-compliance, sleep disturbances, consumption of alcohol and energy drinks during therapy are factors that can be controlled during the physician’s individual work with the patient aimed at the explanation and prevention of risks in treatment.

Evaluation of Toxic Effects of Caffeinated Energy Drinks on Thyroid Gland of Adult Male Albino Rats: Assessment of Apoptosis and Regeneration

Evaluation of Toxic Effects of Caffeinated Energy Drinks on Thyroid Gland of Adult Male Albino Rats: Assessment of Apoptosis and Regeneration

Evaluation of the effectiveness of pomegranate juice on some biochemical and tissue variables in male rats treated with Red Bull energy drink

Evaluation of the effectiveness of pomegranate juice on some biochemical and tissue variables in male rats treated with Red Bull energy drink

Randomized study to assess colonic microbiome changes in response to energy drink consumption (ROSANNA trial).

Randomized study to assess colonic microbiome changes in response to energy drink consumption (ROSANNA trial).

Beyond the buzz: the fatal consequences of caffeine overconsumption.

Caffeine is a naturally occurring stimulant present in dozens of plant species including Coffea arabica and Camellia sinensis, from which we obtain coffee and tea, respectively. It is one of the world's most widely consumed psychoactive substances frequently used to increase alertness, elevate mood, and ward off fatigue. In traditional preparations, caffeine is generally well-tolerated by the consumer. However, complications can arise with the addition of caffeine to products like energy drinks, medications, and supplements. Furthermore, with pure caffeine accessible online, a consumer may unknowingly or inadvertently consume caffeine in dangerous amounts. Symptoms of caffeine toxicity include classic central nervous system stimulation side effects, such as agitation, insomnia, gastrointestinal distress, tachycardia, seizures, and death in extreme cases. To evaluate concentrations of toxicological significance, caffeine cases were assessed at a large reference laboratory (NMS Labs). From 2019 to 2023, 406 blood cases underwent confirmation testing via LC-MS-MS; the mean and median caffeine concentrations were 35 and 4.8 µg/mL, respectively. While most caffeine-containing cases indicate traditional use in the general population with concentrations <25 µg/mL (62%, N = 254), 10% (N = 42) of the cases were >100 µg/mL, indicating levels which may contribute to a fatal outcome. To gain insight into the significance of caffeine in determining the cause and manner of death, cases with various manners of death are presented. Despite being one of the most common toxicological findings in medicolegal death investigations, caffeine is often overlooked. Screening results should undergo scrutiny, and confirmation testing should be considered in cases where caffeine intoxication is prominently featured in the case history or scene investigation.

Immunogystochemical (Sd-138 Marker) Results Of Pathomorphological Changes In The Uterus When Drinking Energy Drinks Chronically Under Experimental Conditions

In this study, the experimental method examined the morphological changes that occur in uterine tissue and glands when white non-breeding rats are ingested in chronic energy drinks in an immunogystochemical manner. From micropreparations made from uterine tissue structures isolated from white non-breeding bats, immunogystochemical testing was performed using the CD-138 marker to produce data on the results obtained based on the degree of expression of the same marker

Validation of an Arabic version of the short form of the health literacy in dentistry scale: a cross-sectional study

BackgroundThe Arabic version of the short form of the Health Literacy in Dentistry scale (HeLD) had not yet been developed in previously published studies. This study aims to test the reliability and validity of an Arabic version of the short form of the HeLD questionnaire.MethodsThe short version of HeLD was translated into Arabic and the psychometric properties were evaluated in a sample of 1,889 female students in their first year of secondary school. Test–retest reliability was assessed using the intra-class correlation coefficient (ICC), and internal consistency reliability was assessed using Cronbach’s alpha. Content validity was investigated by creating a correlation matrix between the individual items of the HeLD-14, and criterion validity was determined using Pearson’s correlation between the HeLD-14 score and an overall oral health rating. Sensitivity analysis was assessed by testing the associations of the HeLD-14 score with oral health-related behaviours and residential area.ResultsThe Arabic version of HeLD-14 (A-HeLD-14) had acceptable ICC (0.54) and excellent internal consistency (Cronbach’s alpha: 0.92). The correlations between the items of the A-HeLD-14 varied from 0.3 to 0.9. The A-HeLD-14 showed a statistically significant correlation with the overall oral health rating (r = 0.37, p < 0.001). The median A-HeLD-14 score was significantly higher in participants who brushed their teeth frequently (51.31), visited the dentist regularly (52.00), consumed fresh fruit frequently (51) and consumed soda or energy drinks infrequently (51.00) than participants who brushed their teeth infrequently (41.50), visited the dentist irregularly (49.00), consumed fresh fruit infrequently (47) and consumed soda or energy drinks frequently (48.00).ConclusionThe A-HeLD-14 instrument demonstrates sufficient validity, reliability, and sensitivity for measuring oral health literacy among the Arabic-speaking population.

The Relationship Between Energy Drinks A and B with Blood Pressure and Pulse Rate Increase Among College Students

The Relationship Between Energy Drinks A and B with Blood Pressure and Pulse Rate Increase Among College Students

Association between Low Calorie Sweetener Consumption Pattern, Anthropometric Indices, and Nutritional Status among Adults’ Males and Females in Calabar Municipality, Nigeria

Aim: This study aimed to investigate the association between low-calorie sweetener (LCS) consumption patterns, anthropometric indices, and nutritional status among adults and women in Calabar Municipality, Nigeria. Study Design; A cross-sectional Analytical design was conducted among 335 participants, selected by multistage sampling technique. Methodology: Dietary assessment using 24hrs recall, anthropometrics measures and socio-demographic characteristics data were collected using pretested questionnaire. The collected data were entered in to Microsoft Excel and exported to SPSS for further statistical analysis. Chi-square test at 95% confidence interval (CI) was used to quantify independent variables of nutritional indices. Using WHO-reference-2010, height, weight and BMI were measured Results: Over 40 products containing LCS were classified into five groups. Carbonated beverages were most consumed, while energy drinks were least consumed. Aspartame was the most commonly used LCS. Young single adults aged 20-35, mostly students, consumed LCS-containing products more frequently than other adults. The overall prevalence of LCS consumption was 58.1%, (moderate), with participants consuming an LCS containing beverage, at least three times a week at an estimated quantity of 1 liter. There was statistical significance (P=.05) differences in weight, waist circumference and hip circumference between men and women. The BMI for men and women were of 22.6 ± 0.9 and 21.0 ± 1.0 kg/m2, respectively. The BMI categories of the study participants were as follows; underweight (3.9%), normal weight (65.7) overweight (28.1) and obese (2.3%). The prevalence of abnormal waist-to-hip ratio was reported, with a significant (P=.05) association between LCS consumption and BMI. Bivariate analysis of LCS consumption and socio-demographic variables found significant association between variables such as gender, age, monthly income and educational level at P=.05. Multivariate logistic regression analysis identified age, income, and BMI as predictors of LCS intake. Conclusion: The study showed that the consumption pattern exists among the adults in terms of age category and gender. Consumption of LCS beverages was mostly moderate, on average around thrice per week and was influenced by demographic factors such as gender, age, and socioeconomic status.

Selective separation of lead (II) from foodstuffs and environmental samples by adsorption onto modified truffle peels with nanoparticles [TPs/TiO2(NP)] as a novel biomass

ABSTRACT This study aims to examined truffle peel powder modified with nanoparticles [TPs/TiO2(NP)] as a solid adsorbent for the removal of lead ions Pb(II) from environmental media and foodstuffs. The characterisation of TPs/TiO2(NP) was studied by a Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and energy dispersive X-ray (EDX). Through the application of several adsorption isotherm models, the Langmuir at 25°C revealed to be most appropriate, having the highest R2 (0.961), and the maximum Pb(II) adsorption capacity was found 0.908 mg g−1. In addition, the various kinetics models were applied, and the data was well-fitted by the pseudo-second order kinetics model (R2 = 0.9997). Thermodynamic parameters indicated that the technique of adsorption had been endothermic (∆H = 30.79 J mol−1), The value of ΔG indicates the spontaneous and physical nature (- 29.26 KJ mol−1) and the positive value of ∆S indicates a moderate step of sorption of the Pb(II) complex ion associate (98.32 J). Foodstuffs (energy drinks and ice pops) and environmental samples (tap water) were successfully confirmed with a good recovery.