Ion mobility spectrometry (IMS) coupled with mass spectrometry (MS) enables the investigation of protein folding in solution. Herein, a proof‐of‐concept for obtaining structural information about the folding of a protein in dependency of the amount of an organic cosolvent in the aqueous medium by means of this IMS‐MS method is presented. By analyzing the protein with native nano‐electrospray ionization IMS‐MS, the impact of acetonitrile as a representative organic cosolvent and/or pH values on the folding of an enzyme was successfully evaluated in a fast and straightforward fashion, as exemplified for an ene reductase from Gluconobacter oxydans. The IMS‐MS results are in agreement with findings from the nicotinamide adenine dinucleotide phosphate (NADPH)‐based spectrophotometric enzyme activity tests under analogous conditions, and thus, also rationalizing these “wet” analytical data. For this ene reductase, a higher tolerance against CH3CN in the presence of a buffer was observed by both analytical methods. The results suggest that this IMS‐MS methodology could be a useful complementary tool to existing methods in process optimization and fine‐tuning of solvent conditions for biotransformations.