AbstractThe technique of radioimmunoassay following sample resolution by HPLC was used to assay the amounts of the cytokinins zeatin (Z), zeatin riboside (ZR) and isopentenyladenine (IPA), the combined amounts of gibberellins1+3(GA1+3), and the amounts of indole acetic acid (IAA) and abscisic acid (ABA) during germination in grains of sorghum. Concentrations of GA1+3were low throughout germination and did not appear to be related to the time of germination. In the mature, non-germinated grain, the concentration of each of the other plant growth regulators was much higher in the smaller component comprised of the embryonic axis and scutellum than in the much larger endosperm tissue. During the germination period studied (64 h), these concentrations declined, with a peak in the amount of the cytokinin IPA and a small peak in Z+ZR (24 h) in the embryo following the first visible signs of root protrusion and coincident with a large enhancement in amylase activity. The high concentration of ABA in the embryo tissue prior to germination was noteworthy. It is suggested that the interaction of ABA and the cytokinins IPA and Z+ZR may play a significant role in controlling sorghum germination.