In the present study brain laterality, hemispheric communication, and mental rotation performance were examined. A sample of 33 women were tested for a possible linear relationship of testosterone level and mental rotation with structural background of the brain. Subjects with a smaller splenial area of corpus callosum tended to have lower levels of testosterone (r =.37, p<.05). However, there were no significant differences in mean scores of mental rotation of object and hand between groups with high and low levels of testosterone. There was a significant difference in relative size of the 6th area (slice) of the corpus callosum between groups with good and poor scores on mental rotation of an object and also in relative size of the 4th and 5th slices of the corpus callosum between groups on mental rotation of the hand. The good and poor scorers' show different relations with the measures of the corpus. The mental rotation of hand was associated with the parietal areas of the corpus callosum, while the mental rotation of object was associated only with the occipital area. These observations suggest that higher testosterone levels may be associated with a larger splenial area, which represents an important connection between the parieto-occipitocortical areas involved in activation of mental images. Further srudy is encouraged.