This study aims to investigate and compare the establishment of dentinal cracks during root canal preparation using a stereomicroscope and a scanning electron microscope (SEM). Sixty removed human mandibular premolars were separated into three groups of 20: two experimental and one control. Within the randomized controlled experimental groups, root canals were constructed. Group I: the Waldent walflex file and Group II: the Trunatomy (TRN) file. Group III: the control group received no preparations. The surfaces of the roots werechecked for dentinal cracksusing a stereomicroscope and SEM following sectioning at 3, 6, and 9 mm from the apex.The Chi-square test was used to examine the data. In the control group, no fissures appeared. Cracks in Waldent Walflexwere almost 66.7%between the 3mm and 6mm range. At both the 6 mm and 9 mm levels, there was no statistically significant difference between the experimental groups (P > 0.05).Defects were found to be substantially higher in the apical region of samples than in the median and coronal sections. Dentinal fissures were created by every rotary file used in the tests. There were more flaws in the Waldent Walflex file group than in the Trunatomy file group.