Spindle Endings Research Articles

Optimization of the Control Algorithm for an Active Bearing Support

This article presents a spindle system with an active bearing support for which the author proposed a control system based on a PID controller. This type of solution improves the mechanical properties of this spindle by changing the stiffness, which results in a wider range of permissible rotational speeds, lower power losses and an increased resistance to vibrations of the entire system. This work describes the mechanism of changing the bearing preload. During tests on a real test stand, the correct functioning of the preload control system was checked for various dynamic conditions. In this work, the author describes the controller used to work in a feedback system, explains how the settings for this controller were selected and their optimization. The assessment of the correct operation of the controller is based on minimizing the amplitude of forced vibrations for the front spindle end by actively controlling the preload of the bearings. This publication is intended to present one of the ways to solve the important problem of limiting the maximum values of the vibration amplitude in the spindle system for its wide range of operating conditions. It also shows how important its optimal settings are in control systems.

Dynamic Modeling of Motorized Spindle System with Unbalanced Mass and Spindle Inclination

The high-speed motorized spindle has been wildly used in the field of aerospace processing, due to its advantages such as high speed, high precision, and high efficiency. CNC machine tools used for processing aerospace products require high machining accuracy, and once the spindle fails, it will seriously affect the quality of product processing. Thus, it is important to study the faults of the spindle, especially the faults caused by subtle errors. In this work, a dynamic model of a spindle with unbalanced mass fault and spindle inclination fault is established, and the natural frequencies and mode shapes of the motorized spindle are calculated by using the whole transfer matrix method (WTMM). The deflections of the spindle initial end in the different situations are discussed when the two faults happen independently. The results show that the spindle end deflection of the same fault has different sensitivity at different speeds. At the third order of natural frequencies, the deflection of a motorized spindle is greatest regardless of the fault that occurs. Although the motorized spindle rotates at the same speed, different faults could cause different mode shapes. At the lower speed, when the unbalanced mass fault happens, the mode shape is in an arched shape, and while the spindle inclination fault happens, the mode shape is in a concave shape.

The contribution of muscle spindles to position sense measured with three different methods

The sense of limb position is important, because it is believed to contribute to our sense of self-awareness. Muscle spindles, including both primary and secondary endings of spindles, are thought to be the principal position sensors. Passive spindles possess a property called thixotropy which allows their sensitivity to be manipulated. Here, thixotropic patterns of position errors have been studied with three commonly used methods of measurement of position sense. The patterns of errors have been used as indicators of the influence exerted by muscle spindles on a measured value of position sense. In two-arm matching, the blindfolded participant indicates the location of one arm by placement of the other. In one-arm pointing, the participant points to the perceived position of their other, hidden arm. In repositioning, one of the blindfolded participant’s arms is placed at a chosen angle and they are asked to remember its position and then, after a delay, reproduce the position. The three methods were studied over the full range of elbow angles between 5° (elbow extension) and 125° (elbow flexion). Different outcomes were achieved with each method; in two-arm matching, position errors were symmetrical about zero and thixotropic influences were large, while in one-arm pointing, errors were biased towards extension. In repositioning, thixotropic effects were small. We conclude that each of the methods of measuring position sense comprises different mixes of peripheral and central influences. This will have to be taken into consideration by the clinician diagnosing disturbances in position sense.

Transient thermal properties investigation for precision bearing-spindle system considering fixed-position preload and lubricant viscosity-temperature effect

Estimating the thermal properties of the precision bearing-spindle system is essential for machine tool operating accuracy and service life. To predict transient temperature field and related heat behavior exactly, an improved thermal network model for the spindle system considering fixed-position preload and lubricant viscosity-temperature effect was proposed. Firstly, the thrust load-axial displacement relationship of angular contact ball bearing was deduced, as well as the difference of bearing inner/outer ring thermal displacement in condition of Double O configuration, and the structural deformation for the spindle system subject to thermal-mechanical effect. Then, the bearing heat generation via the local approach and thermal resistances were calculated, and heat balance equations were derived and solved. Finally, the transient model coupled with heat generation/transfer and structural deformation in service was constructed, and the temperature field and related heat behaviors were obtained. Moreover, the results of theoretical model were compared with those of experiment test, indicating that the bearing temperature rise and spindle end deformation were in good agreement. Meanwhile, the bearing temperature rise, bearing and spindle deformation, and actual preload force were discussed, which had a nonlinear relationship and a positive correlation trend with rotation speed and initial preload. In addition, the assembly status might change with the variation of rotation speed.

A Robotic Spindle End High-Accuracy Positioning Method Based on Eye-in-Hand Vision Active Correction

A Robotic Spindle End High-Accuracy Positioning Method Based on Eye-in-Hand Vision Active Correction

STUDY OF THE INFLUENCE OF THE WORKING LIQUID TEMPERATURE IN THE SPINDLE BEARINGS OF A CYLINDRICAL GRINDING MACHINE ON THE ERRORS OF ITS POSITION RELATIVE TO THE PART BEING MACHINED

The article presents the results of measurements of the ambient temperature around the working cylindrical grinding machines during the working day. The analysis of the behavior of the grinding head spindle of different cylindrical grinding machines during the work shift is performed, namely the results of experimental studies of the displacement of the front and rear ends of the grinding head spindle of cylindrical grinding machines are presented. The analysis of influence of temperature of working fluids in spindle bearings on its displacement is carried out. Possible changes in the geometric shape, namely, conicity, of parts machined on cylindrical grinding machines have been established.

Mitotic spindle disassembly in human cells relies on CRIPT having hierarchical redox signals.

Swift and complete spindle disassembly in late mitosis is essential for cell survival, yet how it happens is largely unknown in mammalian cells. Here we used real-time live cell microscopy and biochemical assays to show that the primordial dwarfism (PD)-related cysteine-rich protein CRIPT dictates the spindle disassembly in a redox-dependent manner in human cells. This previously reported cytoplasmic protein was found to have a confined nuclear localization with a nucleolar concentration during interphase but was distributed to spindles and underwent redox modifications to form disulfide bonds in CXXC pairs during mitosis. Then, it directly interacted with, and might transfer a redox response to, tubulin subunits via a putative redox exchange among cysteine residues to induce microtubule depolymerization. Expression of CRIPT proteins with mutations of these cysteine residues blocked spindle disassembly, generating two cell types with long-lasting metaphase spindles or spindle remnants. Live-cell recordings of a disease-relevant mutant (CRIPTC3Y) revealed that microtubule depolymerization at spindle ends during anaphase and the entire spindle dissolution during telophase might share a common CRIPT-bearing redox-controlled mechanism.

Influences of configuration and coating on the vibration characteristics of spindles

Strong vibration is the main factor leading to the spinning spindle failure, which is closely related to the spindle end configuration and surface coating. In this study, the effects of the spindle end configuration and surface coating on the vibration characteristics of spinning spindles are studied, the vibration tests were conducted on a multifunctional spindle test bench by using three different types of spindles without coating and the YD4203C pointed spindle with surface self-lubricating coating under various high-speed no-load conditions. The results showed that the vibration phenomenon of the YD4203C pointed-head spindle was slighter than that of YD6111FA frustum spindle and YD6100E flat-headed spindle, this was believed to be because the increase in the end surface area could speed up the heat transfer, and the decrease in the bottom area of the spindle end could reduce the impact of the high-speed oil and gas vortex on the vibration. In addition, the vibration characteristics of the coated YD4203C pointed spindles are significantly improved compared with the uncoated spindles. This study provides the experimental basis for the design and manufacture of spindles with low vibration.

Secondary endings of muscle spindles: Structure, reflex action, role in motor control and proprioception.

What is the topic of this review? We describe the structure and function of secondary sensory endings of muscle spindles, their reflex action and role in motor control and proprioception. What advances does it highlight? In most mammalian skeletal muscles, secondary endings of spindles are more or much more numerous than primary endings but are much less well studied. By focusing on secondary endings in this review, we aim to redress the balance, draw attention to what is not known and stimulate future research. Kinaesthesia and the control of bodily movement rely heavily on the sensory input from muscle spindles. Hundreds of these sensory structures are embedded in mammalian muscles. Each spindle has one or more sensory endings and its own complement of small muscle fibres that are activated by the CNS via fusimotor neurons, providing efferent control of sensory responses. Exactly how the CNS wields this influence remains the subject of much fascination and debate. There are two types of sensory endings, primary and secondary, with differing development, morphology, distribution and responsiveness. Spindle primary endings have received more attention than secondaries, although the latter usually outnumber them. This review focuses on the secondary endings. Their location within the spindle, their response properties, the projection of their afferents within the CNS and their reflex actions all suggest that secondaries have certain separate roles from the primaries in proprioception and motor control. Specifically, spindle secondaries seem more adapted than primaries to signalling slow and maintained changes in the relative position of bodily segments, thereby contributing to position sense, postural control and static limb positioning. By highlighting, in this way, the roles of secondary endings, a final aim of the review is to broaden understanding of muscle spindles more generally and of the important contributions they make to both sensory and motor mechanisms.

Automated tracking of S. pombe spindle elongation dynamics.

The mitotic spindle is a microtubule-based machine that pulls the two identical sets of chromosomes to opposite ends of the cell during cell division. The fission yeast Schizosaccharomyces pombe is an important model organism for studying mitosis due to its simple, stereotyped spindle structure and well-established genetic toolset. S. pombe spindle length is a useful metric for mitotic progression, but manually tracking spindle ends in each frame to measure spindle length over time is laborious and can limit experimental throughput. We have developed an ImageJ plugin that can automatically track S. pombe spindle length over time and replace manual or semi-automated tracking of spindle elongation dynamics. Using an algorithm that detects the principal axis of the spindle and then finds its ends, we reliably track the length of the spindle as the cell divides. The plugin integrates with existing ImageJ features, exports its data for further analysis outside of ImageJ and does not require any programming by the user. Thus, the plugin provides an accessible tool for quantification of S. pombe spindle length that will allow automatic analysis of large microscopy data sets and facilitate screening for effects of cell biological perturbations on mitoticprogression.

Design and development of a five-axis machine tool with high accuracy, stiffness and efficiency for aero-engine casing manufacturing

In order to satisfy the machining requirements of aero-engine casing in modern aviation industry, this paper investigates three main issues during the design and development process of a five-axis machine tool with high accuracy, stiffness and efficiency, including whole structure design, key components design, and supporting stiffness design. First, an appropriate structure of five-axis machine tool is determined considering the processing characteristics of aero-engine casing. Then, a dual drive swing head and a compact motorized spindle are designed with enough drive capability and stiffness, and related structure, assembly method, cooling technology, and performance simulation are given in detail. Next, a design method of supporting stiffness of guide is proposed through the deformation prediction of the spindle end. Based on above work, a prototype of machine tool is developed, and some experiments are carried out, including performance tests of swing head and motorized spindle, and machining of a simulated workpiece of aero-engine casing. All experimental results show that the machine tool has satisfactory accuracy, stiffness and efficiency, which meets the machining requirements of aero-engine casing. The main work can be used as references for engineers and technicians, which are meaningful in practice.

Crosstalk opposing view: Independent fusimotor control of muscle spindles in humans: there is little to gain.

Crosstalk opposing view: Independent fusimotor control of muscle spindles in humans: there is little to gain.

Analysis of coupled vibration characteristics of PMS grinding motorized spindle

Field-path-kinetic coupling and the multi-body dynamics models were established and the finite element method (FEM) was used to study the coupled vibrations of motorized spindle. The results show that the frequency is mainly 1 fr at low speeds, fractional-octave and 1fr at high-speed only the bearing force. The orbit of bearing transitions is from a more pronounced circle to an approximately circular shape, while the rotor changes from a rule to a random pattern. The front bearing is more obvious than the rear and the orbits are always round; the radius of the orbit at the front bearing gradually increases as the speed increases. The result is the vibration displacement changes more significantly close to the spindle end and the amplitude increases accordingly. The experimental results show that operating conditions are closer to the normal, the influence of unbalanced magnetic pull can be neglected, the bearing selection and assembly are reasonable.

FABRICATION OF SAND/ZINC OXIDE/TITANIUM DIOXIDE NANOCOMPOSITE AS PHOTOCATALYST

In this work, a nanocomposite photocatalyst was fabricated by growing zinc oxide (ZnO) and titanium dioxide (TiO2) on the sand as a substrate. The initial sand/ZnO was fabricated via sol-gel immersion method for 4 h at 95℃. Furthermore, the sand/ZnO/TiO2 was fabricatedusing hydrothermal method for 5 h at 150℃. Based on field emission scanning electron microscopy (FESEM) analysis, the fabricated sand/ZnO/TiO2 consists of random formation of hexagonal ZnO nanorods and two pyramidal spindle ends of TiO2 nanorods. The addition of TiO2 on top of ZnO nanorods increased the number of active sites which enables more contaminants to be absorbed thus enhanced the photocatalysis process. Moreover, based on the micro-Raman spectra, the synthesized TiO2 was in rutile phase and the ZnO peak was unobservable due to the overlapping with TiO2 peak. Based on its morphological and structural properties, the fabricated sand/ZnO/TiO2 nanocomposite was potential to be applied as photocatalyst.

Firing properties of muscle spindles supplying the intrinsic foot muscles of humans in unloaded and freestanding conditions

We recently developed an approach for recording from muscle spindles in the intrinsic muscles of the foot in freestanding humans by inserting a tungsten microelectrode into the posterior tibial nerve behind the medial malleolus of the ankle. Here we characterize the behavior of muscle spindles in the small muscles of the foot in 1) seated subjects with the leg horizontal and the foot naturally plantarflexed and 2) standing subjects. In the first study, recordings were made from 26 muscle spindle afferents located within flexor digiti minimi brevis ( n = 4), abductor digiti minimi ( n = 3), quadratus plantae ( n = 3), plantar interossei ( n = 4), flexor digitorum brevis ( n = 3), dorsal interossei ( n = 2), and lumbricals ( n = 2), with one each supplying abductor hallucis, adductor hallucis, and flexor hallucis brevis. The identity of another two muscle afferents was unknown. The majority of the units were silent at rest, only seven (27%) being spontaneously active. Because of the anatomic constraints of the foot, some spindles supplying muscles acting on the toes responded to movements of one or more digits. In the second study, 12 muscle spindle afferents were examined during standing. The ongoing discharge of eight spindle afferents covaried with changes in the center of pressure during postural sway. We conclude that the majority of spindle endings in the small muscles of the foot are silent at rest, which may allow them to encode changes in conformation of the foot when it is loaded during standing. Moreover, these muscle spindle afferents can provide useful proprioceptive information during standing and postural sway. NEW & NOTEWORTHY We have characterized the firing properties of muscle spindles in the intrinsic muscles of the human foot for the first time. The majority of the spindle endings are silent in seated subjects, and most fire tonically during standing, their discharge covarying with center of pressure during postural sway. We conclude that spindle endings in the intrinsic muscles of the foot provide useful proprioceptive information during free standing.

Microneurography from the posterior tibial nerve: a novel method of recording activity from the foot in freely standing humans.

The posterior tibial nerve, located behind the medial malleolus of the ankle, supplies the intrinsic muscles of the foot and most of the skin of the sole. We describe a novel approach for recording from this nerve via a percutaneously inserted tungsten microelectrode and provide examples of recordings from presumed muscle spindle endings recorded in freely standing human subjects. The fact that the angular excursions of the ankle joint are small as the foot is loaded during the transition from the seated position to standing means that one can obtain stable recordings of neural traffic in unloaded, loaded, and freely standing conditions. We conclude that this novel approach will allow studies that will increase our understanding of the roles of muscle and cutaneous afferents in the foot in the control of upright posture. NEW & NOTEWORTHY We have performed the first microneurographic studies from the posterior tibial nerve at the ankle. Stability of the recording site allows one to record from muscle spindles in the intrinsic muscles of the foot as well as from cutaneous mechanoreceptors in the sole of the foot during the transition from seated to standing. This novel approach opens up new opportunities for studying the roles of muscle and cutaneous afferents in the foot in the control of upright stance.

Distribution of TTX-sensitive voltage-gated sodium channels in primary sensory endings of mammalian muscle spindles.

Knowledge of the molecular mechanisms underlying signaling of mechanical stimuli by muscle spindles remains incomplete. In particular, the ionic conductances that sustain tonic firing during static muscle stretch are unknown. We hypothesized that tonic firing by spindle afferents depends on sodium persistent inward current (INaP) and tested for the necessary presence of the appropriate voltage-gated sodium (NaV) channels in primary sensory endings. The NaV1.6 isoform was selected for both its capacity to produce INaP and for its presence in other mechanosensors that fire tonically. The present study shows that NaV1.6 immunoreactivity (IR) is concentrated in heminodes, presumably where tonic firing is generated, and we were surprised to find NaV1.6 IR strongly expressed also in the sensory terminals, where mechanotransduction occurs. This spatial pattern of NaV1.6 IR distribution was consistent for three mammalian species (rat, cat, and mouse), as was tonic firing by primary spindle afferents. These findings meet some of the conditions needed to establish participation of INaP in tonic firing by primary sensory endings. The study was extended to two additional NaV isoforms, selected for their sensitivity to TTX, excluding TTX-resistant NaV channels, which alone are insufficient to support firing by primary spindle endings. Positive immunoreactivity was found for NaV1.1, predominantly in sensory terminals together with NaV1.6 and for NaV1.7, mainly in preterminal axons. Differential distribution in primary sensory endings suggests specialized roles for these three NaV isoforms in the process of mechanosensory signaling by muscle spindles.NEW & NOTEWORTHY The molecular mechanisms underlying mechanosensory signaling responsible for proprioceptive functions are not completely elucidated. This study provides the first evidence that voltage-gated sodium channels (NaVs) are expressed in the spindle primary sensory ending, where NaVs are found at every site involved in transduction or encoding of muscle stretch. We propose that NaVs contribute to multiple steps in sensory signaling by muscle spindles as it does in other types of slowly adapting sensory neurons.

Synaptic-like vesicles and candidate transduction channels in mechanosensory terminals.

This article summarises progress to date over an exciting and very enjoyable first 15 years of collaboration with Bob Banks. Our collaboration began when I contacted him with (to me) an unexpected observation that a dye used to mark recycling synaptic vesicle membrane at efferent terminals also labelled muscle spindle afferent terminals. This observation led to the re-discovery of a system of small clear vesicles present in all vertebrate primary mechanosensory nerve terminals. These synaptic-like vesicles (SLVs) have been, and continue to be, the major focus of our work. This article describes our characterisation of the properties and functional significance of these SLVs, combining our complementary skills: Bob’s technical expertise and encyclopaedic knowledge of mechanosensation with my experience of synaptic vesicles and the development of the styryl pyridinium dyes, of which the most widely used is FM1-43. On the way we have found that SLVs seem to be part of a constitutive glutamate secretory system necessary to maintain the stretch-sensitivity of spindle endings. The glutamate activates a highly unusual glutamate receptor linked to phospholipase D activation, which we have termed the PLD-mGluR. It has a totally distinct pharmacology first described in the hippocampus nearly 20 years ago but, like the SLVs that were first described over 50 years ago, has since been little researched. Yet, our evidence and literature searches suggest this glutamate/SLV/PLD-mGluR system is a ubiquitous feature of mechanosensory endings and, at least for spindles, is essential for maintaining mechanosensory function. This article summarises how this system integrates with the classical model of mechanosensitive channels in spindles and other mechanosensory nerve terminals, including hair follicle afferents and baroreceptors controlling blood pressure. Finally, in this time when there is an imperative to show translational relevance, I describe how this fascinating system might actually be a useful therapeutic drug target for clinical conditions such as hypertension and muscle spasticity. This has been a fascinating 15-year journey in collaboration with Bob who, as well as having an astute scientific mind, is also a great enthusiast, motivator and friend. I hope this exciting and enjoyable journey will continue well into the future.

A study of the expression of small conductance calcium-activated potassium channels (SK1-3) in sensory endings of muscle spindles and lanceolate endings of hair follicles in the rat.

Processes underlying mechanotransduction and its regulation are poorly understood. Inhibitors of Ca2+-activated K+ channels cause a dramatic increase in afferent output from stretched muscle spindles. We used immunocytochemistry to test for the presence and location of small conductance Ca2+-activated K+ channels (SK1-3) in primary endings of muscle spindles and lanceolate endings of hair follicles in the rat. Tissue sections were double immunolabelled with antibodies to one of the SK channel isoforms and to either synaptophysin (SYN, as a marker of synaptic like vesicles (SLV), present in many mechanosensitive endings) or S100 (a Ca2+-binding protein present in glial cells). SK channel immunoreactivity was also compared to immunolabelling for the Na+ ion channel ASIC2, previously reported in both spindle primary and lanceolate endings. SK1 was not detected in sensory terminals of either muscle spindles or lanceolate endings. SK2 was found in the terminals of both muscle spindles and lanceolate endings, where it colocalised with the SLV marker SYN (spindles and lanceolates) and the satellite glial cell (SGC) marker S100 (lanceolates). SK3 was not detected in muscle spindles; by contrast it was present in hair follicle endings, expressed predominantly in SGCs but perhaps also in the SGC: terminal interface, as judged by colocalisation statistical analysis of SYN and S100 immunoreactivity. The possibility that all three isoforms might be expressed in pre-terminal axons, especially at heminodes, cannot be ruled out. Differential distribution of SK channels is likely to be important in their function of responding to changes in intracellular [Ca2+] thereby modulating mechanosensory transduction by regulating the excitability of the sensory terminals. In particular, the presence of SK2 throughout the sensory terminals of both kinds of mechanoreceptor indicates an important role for an outward Ca2+-activated K+ current in the formation of the receptor potential in both types of ending.

The Leakage Causes and Improving Measures of CB Series of Gear Oil Pump Based on Mechanical Mechanics

When gear oil pump works, oil chamber is filled with high pressure oil, gear moves between housing and sealing devices and must maintain a certain gap between them, so that leakage is inevitable. In this paper, from structure and working principle of gear pump, the main factors influencing its leakage are spindle end gap, face clearance, radial clearance and the gap of the tooth contact surface. Taking effective measures to control the gear oil pump clearance and improve the working efficiency.