Endemic Plant Species Research Articles

α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.

Obesity, defined as New World Syndrome, causes global health problems and big economic losses. Natural products have gained increasing importance because of their antiobesity potency. The genus Jurinea Cass. with approximately 200 described species worldwide has been traditionally used as a therapeutic agent for colic, fever, gout and rheumatism. The aim of this study was to analyze the volatile components, to determine phenolic compounds and to evaluate α-amylase, α-glucosidase, and lipase inhibitory activities of the endemic plant species Jurinea brevicaulis Boiss. The widely used solid-phase microextraction technique (SPME) was employed for Gas Chromatography-Mass Spectrometry (GC/MS) analysis of the volatile components. Quantitative analysis of phenolic compounds was performed using Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). A total of 19 volatile components were specified and o-cymene (10.60 %), β-bisabolene (9.30 %), and sesquicineole (57.5 %) for different terpenes were described as major components. According to the RP-HPLC analysis, sinapic acid, p-coumaric acid and quercetin were determined for the species. IC50 values of the species were determined as 36.59 ± 2.37 μg/mL and 42.56 ± 2.83 μg/mL for α-amylase and α-glucosidase assays, respectively. IC50 value was found as 50.31 ± 3.75 μg/mL with the lipase inhibition analysis. In conclusion, it has been determined that J. brevicaulis included diverse volatile components, three phenolic compounds with antiobesity effect potential, which highlights J. brevicaulis as the up-and-coming candidate of natural product source to be used against obesity.

Intraspecific divergence of diploid grass Aegilopscomosa is associated with structural chromosome changes.

Aegilopscomosa Smith in Sibthorp et Smith, 1806 is diploid grass with MM genome constitution occurring mainly in Greece. Two morphologically distinct subspecies - Ae.c.comosa Chennaveeraiah, 1960 and Ae.c.heldreichii (Holzmann ex Boissier) Eig, 1929 are discriminated within Ae.comosa, however, genetic and karyotypic bases of their divergence are not fully understood. We used Fluorescence in situ hybridization (FISH) with repetitive DNA probes and electrophoretic analysis of gliadins to characterize the genome and karyotype of Ae.comosa to assess the level of their genetic diversity and uncover mechanisms leading to radiation of subspecies. We show that two subspecies differ in size and morphology of chromosomes 3M and 6M, which can be due to reciprocal translocation. Subspecies also differ in the amount and distribution of microsatellite and satellite DNA sequences, the number and position of minor NORs, especially on 3M and 6M, and gliadin spectra mainly in the a-zone. Frequent occurrence of hybrids can be caused by open pollination, which, along with genetic heterogeneity of accessions and, probably, the lack of geographic or genetic barrier between the subspecies, may contribute to extremely broad intraspecific variation of GAAn and gliadin patterns in Ae.comosa, which are usually not observed in endemic plant species.

Effects of Environmental and Disturbance Factors on Plant Community Distribution in Tropical Moist Afromontane Forests, South-West Ethiopia

This study was carried out to investigate the effects of environmental and disturbance factors on plant community distribution in the Majang Forest Biosphere Reserve (MFBR) in south-west Ethiopia. A systematic sample design was conducted to collect vegetation and environmental factors in four study sites. In a nested plot design, the vegetation data were collected from 140 main plots, i.e., 400 m2 (trees), 25 m2 subplots (shrubs, lianas, seedlings, and saplings), and 1 m2 (herbs), respectively. The plant community classification was performed using agglomerative hierarchical cluster analysis (Ward’s Linkage method) in R software (version 4.0.1). The distribution of plant communities along an environmental gradient was computed using canonical correspondence analysis (CCA). In this study, a total of 15 (9.5%) endemic plant species were recorded in MFBR. Four plant community types were identified, and these were Celtis zenkeri-Blighia unijugata, Pouteria altissima-Lecaniodiscus fraxinifolius, Antiaris toxicaria-Celtis toka, and Dracaena afromontana-Cyathea manniana. Environmental and disturbance factors, such as elevations, slopes, harvesting indexes, soil pH, silt, and herbaceous cover, were the most important for determining plant community distribution in the area. Elevation and slope were found to have a significant (P < 0.05) negative and positive relationship with species diversity and richness, respectively. Therefore, the finding of this study provides baseline information that could be necessary for making further conservation and management in MFBR.

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study.

The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L-1), Naphthaleneacetic Acid (NAA) (1 mg L-1), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L-1) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L-1) and NAA (1 mg L-1). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L-1) and NAA (0.25, 0.50, and 1 mg L-1). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L-1 of BAP and 1 mg L-1 of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L-1. These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat.

Harmonizing multi-stakeholder interests to improve forest conservation in Southern Kenya

The cloud forests of the Taita Hills are valuable refuges for numerous endemic and rare animal and plant species. They also provide various ecosystem services that support subsistence farming. Due to the large-scale destruction and conversion of these species-rich natural forests into subsistence agriculture and plantations of exotic tree species, most of the natural cloud forest habitats have disappeared and ecosystem services have been significantly diminished. Despite numerous conservation initiatives, the destruction of the Taita Hills forest ecosystem continues, which also lowers livelihood quality of the local people. During a workshop with representatives of GOs, NGOs, researchers and representatives of the local community we identified potential factors which are essential to reverse this negative trend. We found that governance structures urgently need to be strengthened, and that the traditional conservation system needs to get transformed and revitalised. The basic prerequisite is a vital communication among generations, especially between the youth and the elders, as well as and improved communication of scientific knowledge to policy makers and the society. Furthermore, it is essential to harmonize stakeholders’ mandates, policies and actions to efficiently restore this unique forest biodiversity hotspot, and to secure livelihood needs for the local people.

Antioxidant and antimicrobial activities of methanol extracts from Adonis paryadrica (Ranunculaceae) – a critically endangered endemic species growing in the Turkish flora

This study was conducted to determine the antioxidant and antimicrobial activities of methanol extract obtained from flower, leaf and root sections of endemic Adonis paryadrica (Boiss.) Kandemir & Aytaç stat. nova. naturally growing in the Turkish flora. The most efficient total phenolic compounds and flavonoid contents were obtained from leaf extract at 21.24 mg GAEs (gallic acid equivalent)/g dw and 54.97 mg REs (rutin equivalent)/g dw, respectively. Among the three different sections of this plant, leaf extracts showed the highest Cupric Reducing Antioxidant Power (CUPRAC) effect with 80.28 µmol TEs (trolox equivalent)/g dw. From the three different sections, the methanol extract of the leaf parts demonstrated strong antibacterial activity against Bacillus subtilis with a 16.1 mm zone diameter. These valuable and current findings from these precious plants, which constitute natural resources in terms of biodiversity, contribute innovative information to the literature on endemic plant species.

Comparative phylogeography reveals the demographic patterns of neotropical ancient mountain species.

Mountains are renowned for their bountiful biodiversity. Explanations on the origin of such abundant life are usually regarded to their orogenic history. However, ancient mountain systems with geological stability also exhibit astounding levels of number of species and endemism, as illustrated by the Brazilian Quartzitic Mountains (BQM) in Eastern South America. Thus, cycles of climatic changes over the last couple million years are usually assumed to play an important role in the origin of mountainous biota. These climatic oscillations potentially isolated and reconnected adjacent populations, a phenomenon known as flickering connectivity, accelerating speciation events due to range fragmentation, dispersion, secondary contact, and hybridization. To evaluate the role of the climatic fluctuations on the diversification of the BQM biota, we estimated the ancient demography of distinct endemic species of animals and plants using hierarchical approximate Bayesian computation analysis and Ecological Niche Modelling. Additionally, we evaluated if climatic oscillations have driven a genetic spatial congruence in the genetic structure of codistributed species from the Espinhaço Range, one of the main BQM areas. Our results show that the majority of plant lineages underwent a synchronous expansion over the Last Glacial Maximum (LGM, c. 21 thousand years ago), although we could not obtain a clear demographic pattern for the animal lineages. We also obtained a signal of a congruent phylogeographic break between lineages endemic to the Espinhaço Range, suggesting how ancient climatic oscillations might have driven the evolutionary history of the Espinhaço's biota.

Phytochemical and biological activity profiles of Thymbra linearifolia: An exclusively native species of Libyan Green mountains

Thymbra linearifolia, a Green Mountain endemic Libyan plant species belonging to the family Lamiaceae, was investigated as the plant's first documented phytochemical and biological evaluation report.The plant is used for bronchitis, cough, asthma, and gastrointestinal ailments, including dyspepsia, colic pain, and diarrhea.The phenolic and flavonoid contents of T. linearifolia were analyzed qualitatively and quantitatively by LC-MS analysis and spectrophotometric assays. These analyses revealed the presence of 60.67 mg/g of GAE (Gallic Acid Equivalents) and 26.79 mg/g of RE (Rutin Equivalents) of the phenolics and flavonoids, respectively, in the aerial parts of the plants’ ethanolic extract. Cyanidin-3-O-rutinoside (2.26 %), pelargonidin-3-O-rutinoside (2.36 %), gallocatechin (2.94 %), taxifolin (1.22 %), kaempferol-3-O-rutinoside (0.81 %), quercetin-3-O-retinosie (1.20 %), luteolin (0.99 %), apigenin (2.0 %), dimethoxy luteolin (2.97 %), and pinobanksin-3-O-acetate (2.56 %) were detected to be the major flavonoids, whereas the syringic acid hexoside (1.38 %), and rosmarinic acid (2.54 %) were among the most abundant phenolic acids in the plant’s extract. The DPPH scavenging activity was measured at an IC50 value of 34.48 μg/mL. In addition,potential reducing, metal chelating, and free radical scavenging potentials were exhibited in the in vitro assays, i.e., ABST, ORAC, FRAP, and MC. The remarkable antioxidant activity of the plant was attributed to its phenolic and flavonoid contents. The plant extract had strong cytotoxic effects on three types of cancer cell lines, i.e., MCF-7, HepG2, and Panc-1, with IC50 values of 24.023, 22.94, and 33.30 μg/mL, respectively. Furthermore, the plant extract also exhibited cytotoxic effects greater than the standard anticancer drug, doxorubicin, against all the tested cell lines. The in-silicobinding studies also demonstrated that the phytochemical constituents of T. linearifolia have a high binding affinity against the epidermal growth factor receptor (EGFR) protein, which supported the anticancer potentials of the plant.The antimicrobial assay conducted with the well-diffusion method revealed the moderate effects of the plant’s extract against Bacillus subtilis, Staphylococcus aureus, Saccharomyces cerevisiae, and Candida albicans. However, weak antimicrobial effects were recorded against the Gram-negative bacteria, Escherichia coli, and Salmonella typhimurium. These findings corroborated the immense health benefits of the plant, and substantiatedthe medicinal value claims of the plant, which is currently under use in different forms as part of the locals’ folk-medicinal chest.

Phytosociological and edaphic parameters of Actaea kashmiriana assemblages in Kashmir Himalaya

Phytosociological analysis and soil physicochemical properties provide the baseline data to understand the overall vegetation composition and structure. In this study, we investigated the species composition and diversity of vegetation assemblages of an endemic plant species Actaea kashmiriana along with the edaphic parameters. We laid randomly selected sampling quadrats across five different sites in Kashmir Himalaya. We employed multiple Pearson's correlation and principal component analysis to investigate the interaction between phytosociological and edaphic parameters. The results revealed that 90 plant species are associated with A. kashmiriana, and Asteraceae, Rosaceae and Lamiaceae were the dominant families. Fragaria nubicola (36.59), Dryopteris blanfordii (34.72), Viola odorata (39.83), Galium aparine (23.70), Trifolium pratense (16.19), Cynodon dactylon (16.94), Urtica dioica (15.29) and Taraxacum officinale (14.25) showed higher Importance Value Index (IVI) and were found to be dominant in A. kashmiriana assemblages. The IVI of A. kashmiriana ranged from 11.24 to 13.76 across the study sites. We found a positive co-occurrence of A. kashmiriana with Viburnum grandiflorum, Arisaema jacquemontii, Bistorta amplexicaulis, Taraxacum officinale, Fragaria nubicola, and Urtica dioica. The soil physicochemical properties significantly correlated with the species diversity indices, with the site having nutrient-rich soils and less disturbance possessing higher species richness and diversity. The findings of our study can help in developing better strategies for effective conservation and habitat restoration of this plant species endemic to Kashmir Himalaya, with learnings for elsewhere in the world.

Exploring soil bacterial diversity in different micro-vegetational habitats of Dachigam National Park in North-western Himalaya

Dachigam National Park (DNP), in Zabarwan mountains of north-western Himalaya constitutes a region of high biodiversity with greater endemism. DNP is known for its unique micro-climate together with distinct vegetational zones providing home to variety of threatened and endemic plant, animal, and bird species. However, studies on soil microbial diversity in fragile ecosystems of north-western Himalaya in general and DNP in particular are lacking. This was thus a maiden attempt to study variations in soil bacterial diversity of DNP with respect to changing soil physico-chemical properties, vegetation, and altitude. Soil parameters depicted significant variations among different sites with highest values for temperature, OC, OM and TN being 22.2 ± 0.75 °C, 6.53 ± 0.32%, 11.25 ± 0.54%, 0.545 ± 0.04% from site-2 (low altitudinal grassland site) in summer and lowest of 5.1 ± 0.65 °C, 1.24 ± 0.26%, 2.14 ± 0.45% and 0.132 ± 0.04% at site-9 (high altitudinal mixed pine site) in winter. Bacterial CFU showed significant correlations with soil physico-chemical attributes. This study led to the isolation and identification of 92 morphologically varied bacteria with the highest (15) from site-2 and lowest (04) from site-9 which post BLAST analysis (via 16S rRNA analysis) depicted presence of only 57 distinct bacterial species under taxonomic phylum, Firmicutes and Proteobacteria. Nine species were widely spread (i.e., isolated from > 3 sites), however, most bacteria (37) were restricted to a particular site. Diversity indices ranged between 1.380 to 2.631 (Shannon–Weiner’s index); 0.747 to 0.923 (Simpson’s index) with highest values for site-2 and lowest for site-9. Index of similarity was highest (47.1%) between riverine sites (site-3 and site-4) whereas two mixed pine sites (site-9 and site-10) showed no similarity.

Plant Species Composition and Conservation Values at Dilla University Botanical and Ecotourism Garden, Dilla, Ethiopia

Ethiopia has a diverse topography with higher plant species composition and estimated to the higher proportion of endemic plant species. Currently, several factors drive natural forest destruction in the country, extensive agricultural land expansion triggered by increasing human population is probably the dominant force. The Dilla University Botanical and Ecotourism Garden was established targeting to rescue threatened flora from extinction, contribute towards conservation, and research on biodiversity and sustainable education as well as ecotourism development. The study was aimed to generate basic scientific information by identifying and documenting the formerly available plant species in to the garden and provide information for farther plant collection planning and research. Systematic sampling method was used to collect the vegetation data from 52 plots of 20 m × 20 m (400 m2) quadrats. To collect data for herbaceous plants, five 1 m × 1 m subplots were laid in each of the main plot, where four were at the corners and one at the centre. Vegetation classification was performed using R-programme version 3.6.1 software packages. Shannon–Wiener Diversity Index was used to calculate species diversity, richness, and evenness. A total of 408 plant species, including trees (30%), shrubs (25%), herb (30%), grass (6%), and 9% of other species, were collected. The identified species belong to 287 genera and 105 families. Out of this, 27 species of plants are endemic to Ethiopia and 72 plant species were screened as a medicinal plant used for the treatment of human diseases. Based on IUCN Red Data List, among the endemic plant species nineteen species were least concern; two were near threatened; two were endangered while four were vulnerable. Three vegetation community types were identified from the hierarchical clustering analysis. The result designates that Dilla University Botanical and Ecotourism Garden has high plant species composition and diversity with a good distribution. The higher composition of this ecologically, economically, and socially important plant species at its early stage makes the garden a unique garden in the country and realize to be a centre for research, education, and tourist destination.

Is the Endangered Species Act living to its full potential? The reassessment of the conservation status and recovery of Macbridea alba Chapm. as a case study

Since 1988, the Cooperative Endangered Species Conservation Fund or “Section 6” fund facilitates partnerships between the U.S. Fish and Wildlife Service and state agencies that aim to provide data pertinent to the recovery of Endangered Species Act (ESA) protected species. Despite the success of these efforts, research for rare plants is chronically underfunded and many species experience long periods of research inactivity that hinders their conservation. One example is Macbridea alba Chapm. (white birds-in-a-nest, Lamiaceae, M. alba from hereon), a federally threatened and state endangered mint endemic to four counties within the Florida panhandle. The species is a candidate for delisting after 30 years of protection under the ESA, however a lack of up-to-date data associated with the species has continually challenged the implementation of effective conservation programs and prolonged the recovery process. The focus of this paper is to review the timeline of recovery goals for M. alba, present a summary of recent research findings (i.e., species distribution models, habitat associations, reproductive ecology), and identify achievements as well as persistent obstacles to recovery and delisting. Our research focused on 5 of 10 recovery actions listed in the recovery plan for M. alba. Our findings provide updated data and make novel contributions to the protection of M. alba that will prioritize and improve management efforts. Overall, our work highlights frequent barriers to the recovery and delisting of rare species, using an endemic plant species as a case-study. Importantly, we outline effective methods for the rapid assessment of at-risk plant species that due to enduring data gaps, face an uncertain future in listing and recovery. We hope our work provides a convincing case demonstrating the critical need for current and expanded ESA funding and encourages a diversity of individuals and institutions to participate in critical rare plant research to swiftly fill research gaps and expedite recovery of some of the rarest plant species across the United States.

Evaluation of Germination under Different Storage Conditions of Four Endemic Plant Species from Ethiopia: Implications for Ex Situ Conservation in Seed Banks

The conservation of endemic plant species is a major concern, as the species are with restricted distribution range. Since in situ conservation alone will not guarantee their maintenance, ex situ conservation measures must be undertaken to support the conservation of these species. Investigation of the impact of the storage environment of seeds gives baseline information. Therefore, this study was conducted to investigate the effect of different storage conditions (room temperature, 4 °C and −10 °C) and different storage periods over one year. Four Ethiopian endemic plant species, namely Euryops pinifolius, Kniphofia foliosa, Lobelia rhynchopetalum, and Solanecio gigas, were considered. Multivariate analyses revealed a significant (p < 0.05) effect of storage condition and period on seed germination. The storage of seeds at room temperature before drying may not be recommended for short-term storage of the studied species, except K. foliosa. After drying of seeds, E. pinifolius, K. foliosa, and L. rhynchopetalum seeds showed high germination percentage (above 80%) after storage even at sub-zero temperatures for one year. The germination percentage of S. gigas stored at room temperature before drying for thirteen months was 60% and that stored at 4 °C was 6%, which indicates that the seeds may be categorized under intermediate storage behavior. The three species can be grouped in the orthodox seed storage category. Research on desiccation screening should be undertaken to predict an optimal combination of storage moisture content and suitable storage temperature to determine storage category with certainty.

Agroforestry as Rehabilitation Strategy System in Orang Kayo Hitam Peat Swamp Park Forest, Jambi - Indonesia

Tropical peat forest ecosystems are unique and have multiple social, economic, and ecological benefits. Factors such as excessive drainage and frequent fires cause peatland degradation. Rehabilitation of deforested and drained tropical peat swamp forests is globally relevant in the context of deforestation and forest degradation. One management system that can be implemented in these conditions combines crops and forest plants (agroforestry). The challenges of the agroforestry application strategy in forest parks are the status of the land, which is a conservation area, and the characteristics of the surrounding community and field conditions. Based on these conditions, it is necessary to conduct this research to support the Orang Kayo Hitam (OKH) forest park development strategy appropriately. The research was conducted in May – June 2021 and used Force Field Analysis based on the opinions of 231 respondents. Based on the research results, it is known that respondents stated that the highest internal driving force is motivation to improve family’s economic from agroforestry products (score 52) and the external one is availability of endemic plants (score 45). The respondent’s opinion about the highest internal inhibiting factor is related to public knowledge of benefits from endemic plant species (score 39), while for external is related to market networks for Non Timber Forest Products (NTFP) market (score 43).

Warming drives poleward range contractions of Beringian endemic plant species at high latitudes

AbstractAimSpecies are expected to disperse poleward in response to climate change. For species that are endemic to the high latitudes, this implies that many in the future would face a “no‐where‐to‐go” situation as they are currently occupying the northernmost portion of the continent. Further, because endemism may arise from a combination of physical barriers, climate and geological history, the persistence of many species may require spatial matching of multiple environmental factors within a limited dispersal space. Thus, it is not clear how endemic species might spatially adjust their distributions in response to climate change and whether there are future climate change refugia for these species.LocationNorthwest North America.TaxaPlants.Time PeriodCurrent and the future (2040).MethodsWe used ensemble bioclimatic models to evaluate drivers and directional patterns of future change in the distributions of 66 North American Beringian and amphi‐Beringian species currently occurring in Alaska and the Yukon. We explored the spatial pattern of species richness, losses and climate change refugia across the region.ResultsMore than 80% of the species showed northward shifts in their latitudinal centroids under intermediate warming and are expected to shift their range northward by more than 140 km on average by 2040. Additionally, more than 60% were projected to experience range contractions and up to 20% of the species would have the potential to expand their ranges by more than 100%.Main ConclusionsSuitable habitat for endemic species in northwest North America is expected to decline significantly, especially for species occupying the Arctic tundra. Although the models identified several potential refugia from future climate change, especially at high latitude and elevation, whether the species would be able to colonize new habitats on their own and/or capitalize sufficiently on in situ refugia remains a pertinent conservation question.

Identifying native endemic plant species in Nilgiris using the interval type 2 q-rung orthopair fuzzy Bonferroni mean operator

Identifying native endemic plant species in Nilgiris using the interval type 2 q-rung orthopair fuzzy Bonferroni mean operator

Non-Native Plants Species and Biodiversity of Socotra Island, Yemen

Socotra Island has significant ecological and economic importance at local, national and global scales. Invasive plant species are often considered as potential competitors of native species due to their usual greater capacity for colonization and expansion. The spread of alien plant species has long been recognized as one of the most significant environmental changes due to its ability to decrease biodiversity and alter ecosystem processes. In areas with homogeneity population of wild invasive species especially Argemone mexicana, 32 random sites were randomly detected. Ninety six Quadrates plots were laid down to study the interaction between the invasive plant and the native species especially with conservation attention of endemic plant species. The main aims is to provide base informatics data to establish comprehensive habitat surveillance programs to enable early detection and rapid response of IAS, prevent both intentional or accidental introductions of harmful invasive alien species to the Socotra Island and reduce the likelihood of alien species invasions by decreasing stresses on vulnerable at-risk ecosystems and species. This study was conducted to identify the different types of invasive species present, the rate and pattern of invasion, its intensity, association of invaders and their habitat preference in the Socotra ecosystem. Vegetation sampled in 96 quadrats, each 10 m _ 10 m; through a combination of random and systematic sampling recorded 42 plant species of 36 genera belonging to 24 families with around 10% was recorded as invasive species. Diversity indices like richness, evenness, Simpson and Shannon-Wiener have been analyzed.

Trail running events contribute microplastic pollution to conservation and wilderness areas

Clothing and footwear designed for trail running shed microplastics (MPs) during use. Trail running events may therefore present a significant source of MP pollution in conservation and wilderness areas. Microplastics may present long-term risks to biodiversity and endemic plant and animal species in such areas. In this study, we used a before-after-control-impact approach to quantify and characterise MP emissions from clothing and shoe outsoles during trail running events. Microplastic deposition on trail surfaces was assessed using both a controlled study and during two public trail running events in New South Wales, Australia (the Duval Dam Buster and the Washpool World Heritage Trail Race). Microplastics were present on trails after all events and included fibres and rubber fragments. Microplastic counts varied considerably depending on trail surface hardness and gradient, and clothing and footwear properties. The controlled study showed running tights (leggings) and shoes with soft rubber outsoles produced more MPs than shirts and hard rubbers. In the trail running events, abrasive wear to shoe outsoles produced an average of 0.3 ± 0.1 to 0.9 ± 0.2 MPs/linear metre/runner, and clothing produced 0.7 ± 0.3 to 2.0 ± 0.3 fibres/linear metre/runner, with fibres accounting for 63–69% of MPs. Microplastic deposition from both footwear and clothing was higher on sloped and rock trail surfaces than flat and soil surfaces. Laser Direct Infrared (LDIR) Imaging indicated the main types of MPs present on trails were polyurethane, polyethylene terephthalate and polyamide. Trail running is increasing in popularity and large-scale events may cause a rapid and significant input of MPs in protected areas. Land managers, event coordinators and outdoor apparel manufacturers could mitigate MP impacts however, by diverting foot traffic around ecologically sensitive areas, capping participant numbers, and developing abrasion resistant clothing and footwear.

Reproductive biology of Actaea kashmiriana, an endemic medicinal plant species from the Himalaya: Implications for conservation and sustainable utilisation

In prioritising conservation and sustainable use of medicinal plants, endemic species merit immediate research priority. Towards this endeavour, understanding the basic reproductive biology of endemic medicinal species is crucial to their successful conservation and sustainable utilisation. In this study, the reproductive biology of Actaea kashmiriana, an endemic medicinal plant species from the Himalaya has been investigated for the first time. The results reveal that species is herkogamous and protoandrous in nature. The pollen grains are prolate spheroidal, and pollen viability ranges from 79.4% to 80%. Under in vitro pollen germination, sucrose, boric acid and calcium nitrate were found to be the key nutrients. Stigma receptivity was maximum on 5th – 7th days of anthesis. Higher pollen-ovule ratio and outcrossing index reflects that Actaea kashmiriana is predominantly outbreeder. The results of pollination experiment show that the species is self-compatible and cross-fertile. Higher fruit set in xenogamous and open pollination treatments under natural conditions suggests cross-pollination as primary mechanism in the species. Presence of sweet nectar, aroma, floral display, and copious amount of pollen serve as main attraction and reward in the species and thereby attracting diverse pollinators, including 13 insect visitors belonging to 7 families and 10 genera. Based on the insect visitation efficiency and the pollen load, Bombus tunicatus, Bombus simillimus, Calliphora vomitoria and Eristalis tenax were found to be the dominant pollinators. Overall, the findings of present study have immediate implications in informing the conservation and sustainable utilisation of this medicinally prized endemic species, and also guide strategies for its habitat restoration in high-altitude Himalaya.

集成生态系统服务与生物多样性的系统保护规划

PDF HTML阅读 XML下载 导出引用 引用提醒 集成生态系统服务与生物多样性的系统保护规划 DOI: 10.5846/stxb202112023396 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金项目（32071650，31670539）；云南省中青年学术和技术带头人后备人才项目（202105AC160070）；云南大学研究生科研创新基金项目（2021Y039） Systematic conservation planning by integrating ecosystem services and biodiversity Author: Affiliation: Fund Project: The National Natural Science Foundation of China (grants 32071650, 31670539); Young and Middle-aged Academic and Technical Leaders Reserve Talent Program of Yunnan Province (202105AC160070); Graduate Research innovation project of Yunnan University (2021Y039). 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:生物多样性和生态系统服务的保护与可持续管理是当今世界面临的重大挑战之一，但如何判识与优化集成生态系统服务与生物多样性保护对象的保护优先区网络，相关研究还很有限。针对"三江并流"区，选取珍稀濒危与特有动植物物种和自然植被类型作为生物多样性保护对象，以调节服务（碳存储、固碳和土壤保持）、文化服务（自然游憩）和供给服务（水源供给）为生态系统服务保护对象。应用系统保护规划方法，首先判识出单一生物多样性和生态系统服务保护优先区；然后，分析这些保护优先区间的相关关系，并选择与生物多样性正相关的生态系统服务类型，判识集成生态系统服务与生物多样性的保护优先区；最后，评估了集成生态系统服务与生物多样性保护优先区在六类已建保护地中的保护状况。结果表明：（1）"三江并流"区多情景规划得到的生物多样性与生态系统服务保护优先区之间均呈正相关关系；（2）与分别针对生态系统服务和生物多样性的规划情景相比，集成生态系统服务与生物多样性保护优先区能够同时对两类保护对象提供最高的保护覆盖率；（3）集成生态系统服务和生物多样性保护优先区占研究区总面积的48.9%，其已建保护地覆盖率为32.5%，说明现有保护地体系仍存在保护空缺。 Abstract:Conservation and sustainable management of biodiversity and ecosystem services are one of the major challenges faced by the world today. However, there is still limited research on how to identify and optimize the portfolio of priority conservation areas (PCAs) that integrate ecosystem services and biodiversity as conservation targets. Using the Three Parallel Rivers Region (TPRR) as a case, this study selected representative conservation features for both biodiversity and ecosystem services; biodiversity conservation features included the rare, endangered and endemic plant and animal species and natural vegetation types; ecosystem-service conservation features included regulation services (carbon storage, carbon sequestration and soil retention), cultural services (natural recreation) and supply services (water supply). We firstly identified the individual portfolio of PCAs for biodiversity and each type of ecosystem services by applying a systematic conservation planning approach, respectively. Then, we analyzed the relationship between these different portfolios of PCAs. We identified the PCAs by integrating biodiversity and ecosystem services that had a portfolio of PCAs being positively correlated with biodiversity PCAs. Finally, we assessed the conservation status of the integrated PCAs within six categories of the existing protected areas. The results showed that:(1) there was a positive relationship between different portfolios of the PCAs identified for biodiversity and each type of ecosystem service in TPRR; (2) In comparison with planning scenarios for ecosystem services and biodiversity separately, the PCAs integrating ecosystem services and biodiversity could simultaneously provide the highest coverage for all biodiversity features and ecosystem services; (3) The PCAs integrating ecosystem services and biodiversity covered 48.9% of the study area, and received 32.5% coverage from the protected areas, indicating that conservation gaps still existed in the existing protected-area system. 参考文献 相似文献 引证文献