The aim of this research was to explore the relations between physical exercises and emotion regulation ability of pilot students in the aftermath of an epidemic, and also to investigate the intermediary role of psychological tolerance, with the aim of further finding measures to prevent and intervene in the development of emotional problems in pilot students, to promote pilot students’ psychological well-being better, and to safeguard aviation safety. This study used a questionnaire to examine 391 flight cadets, and the measurement tools included Physical Exercise Rating Scale, psychological resilience scale, and Emotion Regulation Scale. The results showed that the amount of physical exercise was significantly and positively correlated with emotion regulation ability, and psychological resilience was substantially and positively in correlation to emotion regulation ability; psychological resilience mediated in part by physical activities and pilots' emotion regulation ability, and the effect percentage was 52.63%. In summary, it is concluded that: (1) Physical exercise positively predicts the emotional regulation skills of student pilots, physical exercise has a positive predictive effect on psychological resilience, and psychological resilience has a positive predictive effect on pilot students' emotion regulation ability; and (2) Psychological resilience plays a partially mediating role in the impact between physical exercise and pilot students' emotion regulation ability.