This study was conducted to determine the effects of 4-year nursing education on self-esteem and emotional intelligence levels of nursing students. This descriptive longitudinal study was conducted with 73 nursing students between 2014 and 2018 at a foundation university in Istanbul. The nursing students' self-esteem and emotional intelligence levels increased at the end of the 4-year nursing education. Nursing students' self-esteem scores measured in the 2nd and 4th years, their emotional intelligence scores measured in the 3rd, 1st years, and 4th years were statistically significantly higher. In the light of these results, in addition to the routine educational content, it is recommended that the courses related to self-esteem and emotional intelligence be added to the curriculum or trainings should be organized.