Notropis Atherinoides Research Articles

Network connectivity contributes to native small‐bodied fish assemblages in the upper Mississippi River system

Abstract Effective management and conservation of fishes requires understanding habitat use across multiple life stages while ensuring necessary habitats are both available and accessible. Tributary habitats may play an important role in recruitment and dispersal of fishes in anthropogenically modified rivers such as the Mississippi and Illinois Rivers of the Midwest U.S.A. Identifying source locations that contribute to recruitment of fish populations can determine the importance of connectivity within river networks and pinpoint critical habitats that sustain fish populations. In the Upper Mississippi River System (UMRS), the environments that fish use in early life stages (i.e., natal origin) can be identified using otolith trace element analysis due to stability and distinctness in water chemistry (strontium: calcium [Sr:Ca]) among water bodies that is reflected in otoliths. Here, we used trace element analysis to determine natal origin of six small‐bodied fishes including bullhead minnow (Pimephales vigilax), emerald shiner (Notropis atherinoides), gizzard shad (Dorosoma cepedianum), bluegill (Lepomis macrochirus), orangespotted sunfish (Lepomis humilis), and yellow perch (Perca flavescens) across six reaches of the UMRS (Pools 4, 8, 13, 26, the Open River of the Middle Mississippi River, and the La Grange Pool of the Illinois River). Otolith core Sr:Ca for fishes was quantified using laser ablation inductively coupled plasma mass spectrometry. Using the resulting Sr:Ca chemical signatures of otolith cores, natal origin (tributary, immigrant, or potential resident) was determined for individual fish based on family‐specific relationships between otolith and water chemistries. We found that all species originated from tributaries and other reaches (i.e., were immigrants) to varying extents, which acted as evidence for network connectivity. Specifically, tributaries contributed up to 48% of individuals at a given reach. In certain reaches, Pool 26 and the Open River reach, up to 80% of individuals in a species immigrated from another mainstem river reach. Network connectivity was also important in both upstream and downstream directions. Contributions from network connectivity varied among species: bullhead minnow used less whereas orangespotted sunfish used more network connectivity than when all species were combined. Further, the use of network connectivity varied spatially where individuals captured in Pool 8 and the La Grange Pool less often and those from Pool 26 and the Open River more often originated from network connectivity compared to the whole assemblage across reaches. These results indicate that species' life history traits probably interacted with the physical environment, which differs spatially, to yield observed recruitment source patterns. Our results show that network connectivity contributes to established assemblages of native small‐bodied fishes throughout the UMRS and underscore the role of interjurisdictional management in maintaining network connectivity to sustain fish populations.

Comparisons of oxbow lake fish assemblages in relation to bigheaded carp establishment in the lower White River, Arkansas

BackgroundSince the late 1990s, bigheaded carps (largely silver carp [Hypophthalmichthys molitrix] but also bighead carp [H. nobilis]) have established throughout the lower Mississippi River basin. Using previously studied oxbow lakes in the lower White River basin, Arkansas, we compared current (2017, “post-carp” establishment) fish assemblages to historical (2002, “pre-carp” establishment) fish assemblages. Fish assemblages were comprehensively assessed using multiple gears, including boat electrofishing, mini-fyke nets, and experimental small-mesh gill nets.ResultsT-tests suggested that fish assemblage indices of richness, diversity, evenness, and dominance were often greater (P < 0.05) during the post-carp period as reflected by boat electrofishing and experimental gill nets. However, all indices were generally similar (P > 0.05) between the pre-carp and post-carp period with fish assemblages depicted using mini-fyke nets. Nonmetric multidimensional scaling analyses indicated that fish assemblages differed structurally between pre-carp and post-carp periods. Assemblage differences were linked to both small and large abundance changes for more than 20 species. Abundances of gizzard shad (Dorosoma cepedianum), emerald shiner (Notropis atherinoides), pugnose minnow (Opsopoeodus emiliae), crappies (Pomoxis spp.), bluegill (Lepomis macrochirus), orangespotted sunfish (L. humilis), and yellow bass (Morone mississippiensis) declined between the pre-carp and post-carp periods. Conversely, abundances of weed shiner (N. texanus), pallid shiner (Hybopsis amnis), longear sunfish (L. megalotis), buffalofishes (Ictiobus spp.), and gars (Lepisosteus spp.) generally increased during the same period.ConclusionsAlthough not possible to conclude assemblage shifts were entirely related to bigheaded carps due to the absence of an appropriate reference system where carps did not establish, the wide establishment of these carps is one of the most pervasive changes to have occurred in the lower White River ecosystem during the past two decades. Thus, it is probable to conclude that post-carp establishment observations from this study were at least, in part, attributable to bigheaded carp establishment. Impacts of further range expansions by bigheaded carps in the White River and other lower Mississippi River sub-basins are unclear, though this study suggests probable effects on native fish assemblages, underscoring the need for further research and monitoring.

Unconfined fishway design, implementation, and assessment for the emerald Shiner (Notropis atherinoides) in the Upper Niagara River, New York

An engineered seawall in need of repair along the Upper Niagara River in Buffalo, NY, was previously identified as a hydrodynamic barrier to the migration of a small but important prey fish, the emerald shiner (Notropis atherinoides), and its connection to the upstream environs of Lake Erie. This study proposed a novel unconfined fishway design to be incorporated into existing plans for rehabilitation of the seawall at Broderick Park. The proposed fishway was developed using a two-dimensional depth-averaged numerical model, it was explored by placing the target fish into a physical model, and the constructed demonstration project was assessed using field measurements and observations. The numerical model showed that modular baffles attached to the seawall reduced the nearshore flow velocities to about 0.4 to 0.6 m s−1, which was within the acceptable range of velocities for the upstream migration of the emerald shiners. The physical experiments demonstrated that most emerald shiners chose to swim toward, within, or close to the proposed fishway in the presence of higher flow velocities within the main channel region. The proposed fishway design was integrated into the seawall repair as part of a demonstration project supervised by the US Army Corps of Engineers-Buffalo District. Post-construction assessment of the installation showed that (1) the modular baffles reduced time-averaged flow velocities near the seawall and water surface, (2) while very few shiners were observed swimming along the pre-construction seawall, large numbers were observed swimming upstream within this reduced flow velocity corridor created by the baffles, and (3) the structural integrity of the installation proved to be durable and resilient to extremely harsh environmental conditions. This study explored a new approach to fishway design for the passage of relatively small fish in large rivers, and it effectively melded fishway requirements into seawall restoration in concert with the expectations of multiple stakeholders.

Phylogenomics and classification of Notropis and related shiners (Cypriniformes: Leuciscidae) and the utility of exon capture on lower taxonomic groups.

North American minnows of the Shiner Clade, within the family Leuciscidae, represent one of the most taxonomically complex clades of the order Cypriniformes due to the large number of taxa coupled with conserved morphologies. Species within this clade were moved between genera and subgenera until the community decided to lump many of the unclassified taxa with similar morphologies into one genus, Notropis, which has held up to 325 species. Despite phylogentic studies that began to re-elevate some genera merged into Notropis, such as Cyprinella, Luxilus, Lythrurus, and Pteronotropis, the large genus Notropis remained as a taxonomic repository for many shiners of uncertain placement. Recent molecular advances in sequencing technologies have provided the opportunity to re-examine the Shiner Clade using phylogenomic markers. Using a fish probe kit, we sequenced 90 specimens in 87 species representing 16 genera included in the Shiner Clade, with a resulting dataset of 1,004 loci and 286,455 base pairs. Despite the large dataset, only 32,349 bp (11.29%) were phylogenetically informative. In our maximum likelihood tree, 78% of nodes are 100% bootstrap supported demonstrating the utility of the phylogenomic markers at lower taxonomic levels. Unsurprisingly, species within Notropis as well as Hudsonius, Luxilus, and Alburnops are not resolved as monophyletic groups. Cyprinella is monophyletic if Cyprinella callistia is excluded, and Pteronotropis is monophyletic if it includes Hudsonius cummingsae. Taxonomic changes we propose are: restriction of species included in Alburnops and Notropis, elevation of the subgenus Hydrophlox, expansion of species included in Miniellus, movement of Hudsonius cummingsae to Pteronotropis, and resurrection of the genera Coccotis and Paranotropis. We additionally had two specimens of three species, Notropis atherinoides, Ericymba amplamala, and Pimephales vigilax and found signficant differences between the localities (1,086, 1,424, and 845 nucleotides respectively).

Nonhost species reduce parasite infection in a focal host species within experimental fish communities.

The dilution effect describes the negative association between host biodiversity and the risk of infectious disease. Tests designed to understand the relative roles of host species richness, host species identity, and rates of exposure within experimental host communities would help resolve ongoing contention regarding the importance and generality of dilution effects. We exposed fathead minnows to infective larvae of the trematode, Ornithodiplostomum ptychocheilus in minnow‐only containers and in mixed containers that held 1–3 other species of fish. Parasite infection was estimated as the number of encysted worms (i.e., brainworms) present in minnows following exposure. The results of exposure trials showed that nonminnow fish species were incompatible with O. ptychocheilus larvae. There was no reduction in mean brainworm counts in minnows in mixed containers with brook sticklebacks or longnose dace. In contrast, brainworm counts in minnows declined by 51% and 27% in mesocosms and aquaria, respectively, when they co‐occurred with emerald shiners. Dilution within minnow + shiner containers may arise from shiner‐induced alterations in minnow or parasite behaviors that reduced encounter rates between minnows and parasite larvae. Alternatively, shiners may act as parasite sinks for parasite larvae. These results highlight the role of host species identity in the dilution effect. Our results also emphasize the complex and idiosyncratic effects of host community composition on rates of parasite infection within contemporary host communities that contain combinations of introduced and native species.

Nearshore Fish Species Richness and Species–Habitat Associations in the St. Clair–Detroit River System

Shallow water riparian zones of large rivers provide important habitat for fishes, but anthropogenic influences have reduced the availability and quality of these habitats. In the St. Clair–Detroit River System, a Laurentian Great Lakes connecting channel, losses of riparian habitat contributed to impairment of fish populations and their habitats. We conducted a seine survey annually from 2013 to 2019 at ten sites in the St. Clair and Detroit rivers to assess riparian fish communities, and to identify habitat attributes associated with fish species richness and catches of common species. We captured a total of 38,451 fish representing 60 species, with emerald shiner Notropis atherinoides composing the largest portion of the catch. We used an information-theoretic approach to assess the associations between species richness and catches of 33 species with habitat variables (substrate, shoreline vegetation types, and aquatic macrophyte richness). Sand, cobble, and algal substrates and shoreline vegetation were important predictors of species richness based on a multimodel inference approach. However, habitat associations of individual species varied. This work identified manageable habitat variables associated with species richness, while identifying potential tradeoffs for individual species. Further, this work provides baselines for development and evaluation of fish community and shoreline habitat restoration goals.

Hydroacoustic survey standardization: Inter-vessel differences in fish densities and potential effects of vessel avoidance

Hydroacoustics is used broadly to assess fish populations in marine and freshwater systems. Large-scale surveys often employ multiple vessels to complete a survey. Vessels can be a source of variation in multi-vessel surveys, and accounting for this variation is critical to precise and accurate assessments, whether as indices or measures of absolute abundance. We examined areal and volumetric density estimates, area backscattering coefficient (ABC), and mean target strength (TS) produced by three vessels in Lake Erie along three cross-lake transects in July 2014. Our study showed positive correlation in areal ABC, mean TS, and density among vessels; however, there was an almost 30 % higher density obtained with the largest vessel, on average. As a result, vessel choice could contribute to variation and/or bias in forage fish surveys deploying multi-vessels conducted on Lake Erie. Additionally, in some situations, differential depth patterns of fish contributed to vessel related differences in survey results suggesting a difference in vessel avoidance behaviors among targeted forage species, primarily emerald shiner Notropis atherinoides and rainbow smelt Osmerus mordax. This work demonstrates the potential magnitude of differences among vessels and highlights the need to identify and account for or otherwise control for these effects when monitoring or managing fisheries through standardized hydroacoustic assessments surveys.

Spatial trends and temporal declines in tissue metals/metalloids in the context of wild fish health at the St. Clair River Area of Concern

The St. Clair River, which flows from Lake Huron to Lake St Clair, receives discharges and emissions from numerous petroleum refineries, petrochemical facilities, and other sources. We used a multi-factor ANOVA to test for spatial and temporal changes in concentrations of trace metals/metalloids in homogenates of fish from Lake Huron (reference site) and two river sites - an industrialized region (Stag Island) and downstream Walpole Island/Chenal Écarte. In 2002/2003 and in 2014, we sampled 20 adult fish of each sex of the following species: shorthead redhorse (Moxostoma macrolepidotum), yellow perch (Perca flavescens); we added emerald shiner (Notropis atherinoides; unsexed) in 2014. There was a temporal decline of most metals/metalloids in shorthead redhorse at the river sites. Linear Discriminant Analysis separated the 2014 shorthead redhorse by site. Emerald shiners had higher concentrations of metals/metalloids, apart from mercury, than the other species; those concentrations were highest at Chenal Écarte, which warrants further research. Tissue concentrations of mercury, which declined temporally at Stag Island and Walpole Island, were above the protective guidance for wildlife consumers at all sites, but were lower than predicted to affect fish health. Apart from increased liver size (ANCOVA) in shorthead redhorse at Walpole Island (2014), the health variables in the riverine fish were stable or differed slightly from reference, which indicated good health and reproductive potential. In shorthead redhorse, tissue metals correlated inconsistently with health indices. Further research could ascertain if temporal declines in metals/metalloids in riverine SHRH are benefits of remediation actions or fluctuations with other causes.

Temporal variations in the pelagic fish community of Lake Winnipeg from 2002 to 2019

Lake Winnipeg has undergone extensive changes in environmental conditions and fish community structure during recent decades. The presence of cyanobacteria and invasive species as well as eutrophication, flow regulation, fishing pressure, and climate change all have potential impacts on the native fish community. Since 2002, pelagic forage fish species and early life stages of large-bodied fish species in the pelagic zone in Lake Winnipeg (Manitoba, Canada) have been monitored. Catch data were used to study the temporal variation in species-specific occurrence and catch-per-unit-effort (CPUE) and to analyse the effects of lake condition on species occurrence and CPUE estimates. To account for the temporal variation observed for commonly caught prey species in Lake Winnipeg, we used a Bayesian approach, Integrated Nested Laplace Approximation (INLA), to compare the effects of environmental variables on occurrence and CPUE. The pelagic fish community composition varied among years, and CPUE declined by 2.2-fold between 2005–2008 and 2015–2018. Emerald shiner was the most dominate prey fish species but decreased in the catches since the early 2000’s, while rainbow smelt have almost entirely disappeared from the catches in recent years. Overall, we observed a decrease in CPUE in four of six most caught, pelagic species. Important environmental variables explaining occurrence and CPUE of the six most caught species in the survey were temperature and Secchi, trawl, and water depths. The trawl survey serves as a valuable long-term monitoring tool to study trends in responses of both native and non-native species to ecosystem changes in Lake Winnipeg.

Evidence of continued exposure to legacy persistent organic pollutants in threatened migratory common terns nesting in the Great Lakes

Persistent organic pollutants (POPs) accumulate in the food web of the Great Lakes ecosystem, causing concern that these chemicals play a role in the decline of avian species such as colonial-nesting waterbirds. In this study, samples from four life stages of the common tern (Sterna hirundo), a threatened species in New York State, were collected post-mortem in the Buffalo-Niagara region (United States). Brains (n = 26) and livers (n =27) were analyzed for polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and the insecticide p,p’-dichlorodiphenyltrichloroethane (DDT) and its two metabolites p,p’-dichlorodiphenyldichloroethylene (DDE) and p,p’-dichlorodiphenyldichloroethane (DDD). Detectable concentrations of PCBs and PBDEs were observed in all samples; concentrations of total PCBs ranged from 5.59 to 2,490 ng/g wet weight (ww), total PBDEs ranged from 1.09 to 494 ng/g ww, and DDE metabolites ranged from 0.56 to 637 ng/g ww. Analysis of the primary food source for terns in the Buffalo-Niagara region, emerald shiners (Notropis atherinoides), revealed that all three classes of POPs had similar contaminant profiles in the fish to those in the brains and livers of tern samples. Overall, small chicks contained greater concentrations of pollutants than medium chicks and juveniles, likely from maternal loading to eggs. These results underline the persistence of these legacy contaminants in the Great Lakes, despite their banning, and their biomagnification in threatened species through food web interactions.

Hypoxia’s impact on pelagic fish populations in Lake Erie: a tale of two planktivores

Whether bottom hypoxia has long-lasting consequences for pelagic fish populations remains speculative for most ecosystems. We explored hypoxia’s influence on two pelagic zooplanktivores in Lake Erie that have different thermal preferences: cold-water rainbow smelt (Osmerus mordax) and warm-water emerald shiners (Notropis atherinoides). To assess acute effects, we combined predictive bioenergetics-based modeling with field collections made across the hypoxic season in central Lake Erie during 2005 and 2007. To assess chronic effects, we related fishery-independent and fishery-dependent catches with hypoxia severity and top predator (walleye, Sander vitreus) abundance during 1986–2014. As our modeling predicted, hypoxia altered rainbow smelt movement and distributions, leading to avoidance of cold, hypoxic bottom waters. In response, diets shifted from benthic to pelagic organisms, and consumption and energetic condition declined. These changes were lacking in emerald shiners. Our long-term analyses showed rainbow smelt abundance and hypoxia to be negatively related and suggested that hypoxia avoidance increases susceptibility to commercial fishing and walleye predation. Collectively, our findings indicate that hypoxia can negatively affect pelagic fish populations over the long term, especially those requiring cold water.

Accumulation of trace elements in selected fish and shellfish species from the largest natural carp fish breeding basin in Asia: a probabilistic human health risk implication.

Intake of fish contaminated with non-essential hazardous trace elements poses a significant risk to human health. In this study, trace elements (As, Pb, Cd, Cu, Ni, and Zn) were measured in edible tissues of seven commercially important fish and shellfish species (Otolithoides pama, Pseudapocryptes elongatus, Macrobrachium rosenbergii, Liza parse, Notropis atherinoides, Apocryptes bato, and Rhinomugil corsula) from a natural carp breeding basin, Halda river, Bangladesh. The elements were detected by inductively coupled plasma-mass spectrometry (ICP-MS), and the hierarchy of elemental levels (mg/kg) was Zn (61.56) > Pb (30.45) > Ni (26.81) > Cu (21.09) > As (1.49) > Cd (0.24). Among the analyzed elements, Pb, Ni, and Zn for some fish species exceeded the permissible dietary limit, suggested by national and international agencies. In addition, results of bioaccumulation factor (BAF) indicated that most of the studied species were bioaccumulative in nature (BAFs > 1000), and the mean BAF of elements were found in the following order: Zn (3156.74) > Ni (1629.30) > Cu (1566.77) > As (997.14) > Pb (259.98) > Cd (216.52). However, the species, L. parse being omnivorous represented the highest BAF (stored higher concentrations of metals) as compared to other species. The growth pattern of all the species was negatively allometric, and the health condition of the species varied from poor to good state revealed from the estimated Fulton's condition factor (FC). For the evaluation of health hazards, estimated weekly (EWI), target hazard quotient (THQ), and carcinogenic risk (CR) were calculated for both adults and children. Results of EWI showed As, Pb, and Ni surpassed provisional tolerable weekly intake (PTWI)-recommended guidelines. The non-carcinogenic health effect (TTHQ) might not appear for both types of consumers (as TTHQ < 1), and CRs of all consumers were also in acceptable range (10-6 to 10-4). However, the probabilistic distribution through Monte Carlo simulation revealed that children were more vulnerable to non-carcinogenic (67.3%) and carcinogenic risk effect (47.3%) for Pb. Meanwhile, adults obtained the probability of 0.7% and 36% for THQ and CR effect, respectively, interpreting less vulnerable.

Factors Related to Age-0 Native Fish Catch per Unit Effort in the South Dakota Missouri River Reservoirs

AbstractThe Missouri River in South Dakota was dammed in the 1950s and 1960s, altering the biotic and abiotic factors that potentially affect native species in the mainstem reservoirs. Little research has been conducted regarding what factors regulate age-0 catch per unit effort (CPUE) of native fish species since the damming occurred. Thus, we examined age-0 CPUE for 13 native fish species in the four Missouri River mainstem reservoirs. We analyzed data from 1995 to 2015 in Lake Oahe, 2002 to 2016 in Lake Sharpe, 1998 to 2008 in Lake Francis Case, and 2000 to 2013 in Lewis and Clark Lake. Not all species were sampled in all reservoirs. We evaluated potential environmental (inflow, gauge height, peak flow, precipitation, air temperature) and biological (CPUE of other native species, nonnative species, and predators) factors that have documented impacts on age-0 native fish. Significant relationships existed between age-0 native fish CPUE and both biotic and abiotic factors in all four reservoirs, but were species- and reservoir-dependent. Age-0 CPUE was either stable or increasing in all species examined in all reservoirs except age-0 Emerald Shiner Notropis atherinoides in Lewis and Clark Lake. Age-0 Emerald Shiner CPUE in Lewis and Clark Lake was most supported by biological models (e.g., total age-0 nonnative and adult White Bass Morone chrysops CPUE), and a positive relationship existed between age-0 Emerald Shiner and both factors. We believe our findings provide valuable insight into successful management of native fish populations.

Enhanced inference of ecological networks by parameterizing ensembles of population dynamics models constrained with prior knowledge

BackgroundAccurate network models of species interaction could be used to predict population dynamics and be applied to manage real world ecosystems. Most relevant models are nonlinear, however, and data available from real world ecosystems are too noisy and sparsely sampled for common inference approaches. Here we improved the inference of generalized Lotka–Volterra (gLV) ecological networks by using a new optimization algorithm to constrain parameter signs with prior knowledge and a perturbation-based ensemble method.ResultsWe applied the new inference to long-term species abundance data from the freshwater fish community in the Illinois River, United States. We constructed an ensemble of 668 gLV models that explained 79% of the data on average. The models indicated (at a 70% level of confidence) a strong positive interaction from emerald shiner (Notropis atherinoides) to channel catfish (Ictalurus punctatus), which we could validate using data from a nearby observation site, and predicted that the relative abundances of most fish species will continue to fluctuate temporally and concordantly in the near future. The network shows that the invasive silver carp (Hypophthalmichthys molitrix) has much stronger impacts on native predators than on prey, supporting the notion that the invader perturbs the native food chain by replacing the diets of predators.ConclusionsEnsemble approaches constrained by prior knowledge can improve inference and produce networks from noisy and sparsely sampled time series data to fill knowledge gaps on real world ecosystems. Such network models could aid efforts to conserve ecosystems such as the Illinois River, which is threatened by the invasion of the silver carp.

Tissue contaminants and wild fish health in the St. Clair River Area of Concern – Part 2: Spatial trends and temporal declines in organics

We explored tissue concentrations of polychlorinated biphenyls (PCBs), chlorinated pesticides, and relevant organochlorines and fish health in the following adult wild fish in the St. Clair River Area of Concern (Ontario, Canada): shorthead redhorse (Moxostoma macrolepidotum), yellow perch (Perca flavescens), and emerald shiner (Notropis atherinoides). We collected adult fish from sites within the river's industrial zone (Stag Island), a downstream site adjacent to Walpole Island (Chenal Écarte), and an upstream reference site in Lake Huron in 2002/2003 and 2014. We tested for trends in tissue concentrations of organic contaminants across sites and over time; we assessed the potential effects of contaminants on morphological indicators of fish health across sites by year. Over the 12-year period, the tissue concentrations of most PCBs declined at the river sites, except for some non-legacy PCBs (PCB11 and 185), which increased in yellow perch at Stag Island, a new observation for fish in the St. Clair River AOC. There was little difference between the concentrations of calculated toxic equivalents (TEQs) of the Lake Huron and the St. Clair River fish in 2014, except for emerald shiners from Stag Island which had elevated ΣPCB and TEQs. Each fish species at all sites exceeded the Canadian tissue residue guideline for PCBs for the protection of mammalian wildlife consumers of aquatic biota, but fish-derived TEQs indicated little potential health risk to fish. Over time, hexachlorobutadiene and hexachlorobenzene concentrations increased in some fish at Stag Island by about 8- and 4-fold, respectively, whereas they decreased at other sampling locations. Principal Component Analysis followed by Linear Discriminant Analysis of the 2014 SHRH data suggested that although the fish separated by site, tissue concentrations of PCB and organochlorine contaminants did not have consistent relationships to the morphological health indicators, including egg production in females, which implied the absence of causative relationships.

Elevated algal and sedimentary turbidity alter prey consumption by emerald shiner (Notropis atherinoides)

AbstractAnthropogenic increases to turbidity (suspended particulates) in the water column can alter the underwater visual environment, resulting in disruptions to visual signals in fishes exposed to these conditions. However, dissimilar turbidity types (e.g. sedimentary or algal particles) are expected to influence the visual environment in different ways as they exhibit differing physical characteristics. The main objective of this study was to determine the influence of elevated turbidity on prey consumption in emerald shiner (Notropis atherinoides). A secondary goal was to determine the relationship between visual morphological structures (i.e. eyes and optic lobe of brain) and prey consumption in different visual environments. We tested emerald shiner consumption of Daphnia magna in three turbidity types (sedimentary, algal and sedimentary + algal) at two different levels (20 NTU and 40 NTU) as well as in a clear (&lt;5 NTU) control. We found that prey consumption decreased in high turbidity (40 NTU) across turbidity types relative to the control treatment. Further, consumption was lower in each treatment relative to the control with the exception of the moderate (20 NTU) sedimentary turbidity treatment. This study indicates that for emerald shiner, while high levels of turbidity are likely to suppress foraging, moderate levels of sedimentary turbidity may be slightly beneficial for foraging success. Further, it is likely that increases in algal turbidity via cultural eutrophication are likely to result in reductions in prey consumption.

Diet and isotopic niche of eastern sand darter (Ammocrypta pellucida) near the northern edge of its range: a test of niche specificity

Niche specificity can predispose species to population declines during periods of resource limitation, yet trophic niche specificity is poorly known for many small-bodied freshwater fishes. Applying a two-tiered approach involving stomach content and stable isotope analyses, we examined the diet and trophic niche of the threatened eastern sand darter (Ammocrypta pellucida (Putnam, 1863)) and co-occurring fishes in the Thames River, Ontario, Canada. As with previous studies, stomach content analysis revealed that eastern sand darter consumed a variety of benthic organisms including Chironomidae, Cladocera, Ostracoda, Oligochaeta, and Ephemeroptera; however, proportional contributions of prey groups differed based on stable isotope analysis, highlighting the potential for seasonal variation in prey consumption. Despite evidence of a generalist strategy, stable isotope analysis indicated eastern sand darter exhibited a relatively narrow trophic niche relative to co-occurring fishes. Trophic niche overlap was relatively minor between eastern sand darter and drift-feeding fishes (spotfin shiner (Cyprinella spiloptera (Cope, 1867)), emerald shiner (Notropis atherinoides Rafinesque, 1818), and buffalo sp. (genus Ictiobus Rafinesque, 1820)), but was more evident between eastern sand darter and benthic and benthopelagic fishes (johnny darter (Etheostoma nigrum Rafinesque, 1820) and blackside darter (Percina maculata (Girard, 1859))), indicating that competition with these species may be more likely during periods of prey scarcity.

Visual performance impaired by elevated sedimentary and algal turbidity in walleye Sander vitreus and emerald shiner Notropis atherinoides.

The objectives of this study were to determine the effects of different forms of elevated turbidity on the visual acuity of two native Lake Erie fishes and to assess the response of fishes from different trophic levels to elevated turbidity. Additionally, the role of visual morphology (e.g., eye and optic lobe size) on visual acuity was evaluated across visual environments. Reaction distance, a behavioural proxy for measures of visual acuity, was measured for a top predator, walleye Sander vitreus and a forage fish, emerald shiner Notropis atherinoides. In both S. vitreus (n = 27) and N. atherinoides (n = 40) reaction distance across all types of turbidity (sedimentary, algal, sedimentary + algal; 20 NTU) was approximately 50% lower relative to the clear treatment. Reaction distance was further reduced in algal compared with sedimentary turbidity for wild-caught S. vitreus. Eye and brain morphology also influenced reaction distance across turbidity treatments, such that larger relative eye and brain metrics were positively correlated with reaction distance. This study provides evidence for disrupted visual acuity as a potential mechanism underlying fish responses, such as decreased foraging efficiency, to increased turbidity and further indicates that algal turbidity will probably be more detrimental to visual processes than sedimentary turbidity. With the increasing occurrence and severity of harmful algal blooms due to cultural eutrophication globally, this could have significant implications for predator-prey relationships in aquatic systems.

Morphometric differentiation and gene flow in emerald shiners (Notropis atherinoides) from the lower Great Lakes and the Niagara River

Abstract Differences in habitat (e.g., water velocity, prey, and predator regimes) are a driving force causing adaptive divergence among fish populations. This study used geometric shape analysis to assess morphological differences among emerald shiner (Notropis atherinoides) populations inhabiting the Niagara River, Lake Erie, and Lake Ontario. It was expected that emerald shiners inhabiting the two lakes would have more robust bodies and smaller heads, while river emerald shiners were expected to display more fusiform bodies with larger heads. The results of this study indicate that emerald shiners from Lake Erie and the Niagara River had a more robust form on average than individuals from Lake Ontario. Specifically, emerald shiners collected from Lake Ontario displayed more streamlined bodies and larger heads than emerald shiners collected from Lake Erie and the Niagara River. In addition, this divergence in body shape has apparently occurred despite the lack of distinct genetic differentiation as measured with microsatellite variation. Our results suggest that differences in water velocity alone may not be responsible for phenotypic variation in body shape among these emerald shiner populations, and other factors such as differences in prey or predator regimes are likely involved.

Prey fish communities of the Laurentian Great Lakes: A cross-basin overview of status and trends based on bottom trawl surveys, 1978-2016

Annual bottom trawl surveys were initiated in the 1970s in Laurentian Great Lakes Superior, Huron, Michigan and Ontario and in 1990 in Erie to provide annual assessments of the status and trends of prey fish communities. Native Cisco Coregonus artedi and Bloater C. hoyi dominated the prey fish community of Lake Superior. Prey fish communities in lakes Huron and Michigan were dominated by nonnative Rainbow Smelt Osmerus mordax and Alewife Alosa pseudoharengus for much of 1978-2016, but Bloater was an important species during the 1980-1990s and more recently has become the dominant prey species in these lakes. Alewife dominated the prey fish community of Lake Ontario during all 1978-2016. While nonnatives dominated the prey fish community in Lake Erie, native Emerald Shiner Notropis atherinoides was an important species and occasionally the dominant prey fish after the establishment of Round Goby Neogobius melanostomus in the late 1990s. During the 1980s-1990s Bythotrephes cederstroemi, Dreissena polymorpha, and Dreissena bugensis caused profound changes in Laurentian Great Lakes ecosystems and likely contributed to declines in fish community biomass in lakes Michigan and Huron. The impacts of these invaders were more muted in lakes Erie and Ontario. Lake Superior stands out as the Laurentian Great Lakes success story: Lake Trout Salvelinus namaycush was restored, and native prey fishes dominate and support a viable fishery. Although the abundance of Bloater has increased recently in lakes Huron and Michigan, recovery of native prey fishes remains uncertain. The absence of native species among the principal prey fish in Lake Ontario indicates a lack of progress in native fish recovery. Recovery of native prey fishes remains unclear in Lake Erie. The ever-changing state of the Laurentian Great Lakes caused by the impacts of invasive species and ongoing climate and ecosystem change will continue to challenge restoration of native fish communities in the 21st Century.