Embryonic Chick Intestine Research Articles

Differentiation of Argyrophil and Argentaffin Cells in Organotypic Cultures of Embryonic Chick Intestine

ABSTRACT Argyrophil, Bodian positive cells, and argentaffin, Masson-Hamperl positive cells differentiate in vitro in organotypic cultures of intestine of chick embryos explanted before argyrophil and argentaffin cells are known to differentiate, which is at the 14th and the day of incubation, respectively. During culture in vitro, argyrophil and argentaffin granules differentiate simultaneously; the number of cells demonstrable with either method does not show any significant difference throughout the duration of the cultures, which were incubated up to a maximum of 6 days. This result is at variance with what occurs in vivo, where argyrophil cells differentiate earlier than argentaffin cells and are in earlier stages 3 or 4 times as frequent as the latter, becoming only twice as numerous at a later stage. As in development in vivo, argyrophil and argentaffin cells differentiating in the intestine of the chick embryo are mainly situated in the epithelium. Only very few are found in the connective tissue of the lamina propria. Simard & van Campenhout (1932) and Ghidini (1940) showed that enterochromaffin granules differentiate in chorioallantoic grafts of intestine of chick embryos and they disproved in this way any kind of derivation of the enterochromaffin substance (enteramine) from the lumen of the gastrointestinal tract, as well as its formation under the influence of normal nervous connexions. It is now shown that the specific granules differentiate also in intestine cultivated in vitro and therefore in conditions which exclude any other possible mode of correlation with the whole organism, and particularly any uptake of enteramine from the blood, thus strongly supporting the conclusion that it is synthesized in situ.

Functional Differentiation of Small Intestine. VI. Transient Accumulation of Glycogen in Intestinal Epithelium of Chick Embryo under Normal Conditions and under Influence of Hydrocortisone

Previous articleNext article No AccessFunctional Differentiation of Small Intestine. VI. Transient Accumulation of Glycogen in Intestinal Epithelium of Chick Embryo under Normal Conditions and under Influence of HydrocortisoneFlorence Moog and Edgar R. ThomasFlorence Moog Search for more articles by this author and Edgar R. Thomas Search for more articles by this author PDFPDF PLUS Add to favoritesDownload CitationTrack CitationsPermissionsReprints Share onFacebookTwitterLinkedInRedditEmail SectionsMoreDetailsFiguresReferencesCited by Volume 30, Number 4Oct., 1957 Article DOIhttps://doi.org/10.1086/physzool.30.4.30152210 Citations: 14Citations are reported from Crossref Journal History This article was published in Physiological Zoology (1928-1998), which is continued by Physiological and Biochemical Zoology (1999-present). PDF download Crossref reports the following articles citing this article:Betty L Black Development of glucose active transport in embryonic chick intestine. Influence of thyroxine and hydrocortisone, Comparative Biochemistry and Physiology Part A: Physiology 90, no.33 (Jan 1988): 379–386.https://doi.org/10.1016/0300-9629(88)90205-8Phyllis M. Wise, B. E. Frye Functional development of the hypothalamo-hypophyseal-adrenal cortex axis in the chick embryo,Gallus domesticus, Journal of Experimental Zoology 185, no.33 (Sep 1973): 277–291.https://doi.org/10.1002/jez.1401850302D.E. Hart, T.W. Betz On the secretory ability of the grafted pars distalis in chick embryos, General and Comparative Endocrinology 19, no.22 (Oct 1972): 225–241.https://doi.org/10.1016/0016-6480(72)90101-3 REFERENCES, Acta Physiologica Scandinavica 83 (Apr 1971): 88–94.https://doi.org/10.1111/j.1748-1716.1971.tb05126.xRobert M. Pratt, Charles Terner Development of amino acid transport by the small intestine of the chick embryo, Biochimica et Biophysica Acta (BBA) - Biomembranes 225, no.11 (Jan 1971): 113–122.https://doi.org/10.1016/0005-2736(71)90290-2Dorothea L. Baxter-Grillo Enzyme histochemistry and hormones of the developing gastrointestinal tract of the chick embryo, Histochemie 19, no.11 (Mar 1969): 31–43.https://doi.org/10.1007/BF00305960Peter G. Toner Cytology of Intestinal Epithelial Cells, (Jan 1968): 233–343.https://doi.org/10.1016/S0074-7696(08)61401-1W.O. Dobbins, J.C. Hijmans, K.S. McCarty A Light and Electron Microscopic Study of Duodenal Epithelium of Chick Embryos Cultured in the Presence and Absence of Hydrocortisone, Gastroenterology 53, no.44 (Oct 1967): 557–574.https://doi.org/10.1016/S0016-5085(19)34180-0Lewis W. Taylor, G. Oscar Kreutziger The Gaseous Environment of the Chick Embryo in Relation to Its Development and Hatchability, Poultry Science 45, no.55 (Sep 1966): 867–884.https://doi.org/10.3382/ps.0450867David L. Stocum The effects of thiourea and cortisone acetate on duodenal length and diameter and on morphogenesis of duodenal villi in chick embryos, Journal of Morphology 118, no.22 (Feb 1966): 183–195.https://doi.org/10.1002/jmor.1051180204Robert C. Thommes, John J. Just Endocrine control of yolk sac membrane glycogen levels in the developing chick embryo, General and Comparative Endocrinology 4, no.66 (Dec 1964): 614–623.https://doi.org/10.1016/0016-6480(64)90071-1 REFERENCES, Acta Physiologica Scandinavica 62 (Dec 1964): 82–91.https://doi.org/10.1111/j.1748-1716.1964.tb04025.xJohn B. Hinni, Ray L. Watterson Modified development of the duodenum of chick embryos hypophysectomized by partial decapitation, Journal of Morphology 113, no.33 (Nov 1963): 381–425.https://doi.org/10.1002/jmor.1051130306Florence Moog The functional differentiation of the small intestine IX. The influence of thyroid function on cellular differentiation and accumulation of alkaline phosphatase in the duodenum of the chick embryo, General and Comparative Endocrinology 1, no.5-65-6 (Dec 1961): 416–432.https://doi.org/10.1016/0016-6480(61)90006-5