Elution Solution Research Articles

Application of recycled ferric chloride for alkalinity regulation of bauxite residue

Bauxite residue is the biggest environmental issue for the alumina industry, and alkalinity regulation is the key step to treat this kind of solid waste. In this work, recycled ferric chloride, generated from the resource recycling process of bauxite residue, was applied to regulate the alkalinity of bauxite residue. The results show that the pH value of the leachate decreased from 11.38 to 7.06 under the optimal condition (solid-to-liquid ratio 400 g/L, Fe3+ concentration 10 g/L, reaction temperature 25 °C and reaction time 1 h). Most alkaline components in the bauxite residue can be neutralized after two dealkalization stages. Fe3+ showed a preferential exchange capability to Na + than Ca2+, and the exchanged Ca2+ was also beneficial for the alkalinity regulation process. The elution solution may be used as the raw material for chlor-alkali industry. Furthermore, evaluation of the revegetation potential showed that the dealkalized bauxite residue was suitable for the growth of Lolium perenne. In summary, utilization of recycled ferric chloride to regulate the alkalinity of bauxite residue offers an economical and environmentally friendly way for large-scale bauxite residue disposal.

Determination of caffeine content in Ginkgo Folium by ultra high performance liquid chromatography-triple quadrupole mass spectrometry

In this experiment, an ultra-high performance liquid chromatographytandem triple quadrupole mass spectrometry was established for the determination of caffeine in commercially available Ginkgo Folium. The samples were extracted by ultrasonic method with methanol, and separated on Waters CORTECS T3 column(2.1 mm×100 mm, 2.7 μm), with mobile phase of 0.1% formic acid solution-0.1% formic acid acetonitrile solution for gradient elution, at flow rate of 0.3 mL·min~(-1); column temperature of 30 ℃, and injection volume of 2 μL. Mass spectrometry was conducted at ESI~+ multiple reaction monitoring(MRM) mode; quantitative analysis was conducted with external standard method. The results showed that in the range of 0.099 6-9.96 ng·mL~(-1), there was a good linear relationship between the mass concentration of caffeine and the peak area, R~2=0.999; the average recovery was 84.51%, with RSD of 6.2%. The results of precision, repeatability and stability showed that the RSD was 5.1%, 5.9%, 7.2%, respectively. The content range of caffeine in 10 batches of Ginkgo Folium was 1.52-60.86 μg·kg~(-1). In conclusion, this method is accurate, reliable and reproducible, which provides a reference for the safety study of Ginkgo Folium.

Facile and simple purification method for small extracellular vesicles obtained from a culture medium through cationic particle capture.

Although small extracellular vesicles (sEVs) carry DNA, miRNA, and proteins, and they play an important role in long-distance intercellular communication, their generation and circulation mechanisms are unclear. sEVs can be used as biomarkers for the early diagnosis of diseases (e.g., cancer, Alzheimer's disease, melanoma, and cardiovascular diseases) and as drug delivery carriers to the target tissues. Hence, sEVs are attracting considerable attention from scientists and medical professionals. In the present study, we investigated four different commercially available cationic particles (two silica particles modified with diethylaminopropyl or trimethylaminopropyl groups, and two agarose particles modified with diethylaminopropyl or trimethylaminopropyl groups) for the purification of sEVs obtained from a cell culture medium. All the cationic particles captured the sEVs well. The NaCl concentrations required for elution of the captured sEVs differed for the different cationic particles. sEVs were most efficiently captured by silica particles modified with diethylaminopropyl groups, and they were eluted from these particles using 200mM NaCl as the elution solution. Because the developed method can be used to easily purify sEVs obtained from a culture medium, it is expected to facilitate the functional analysis of sEVs, as well as early diagnosis and treatment of diseases using sEVs.

Nuclear magnetic resonance immunoassay of tetanus antibodies based on the displacement of magnetic nanoparticles.

A nuclear magnetic resonance (NMR) immunoassay based on the application of carbon-coated iron nanoparticles conjugated with recognition molecules was designed. The principle of the assay is that ELISA plates are coated with a capture element, and then an analyte is added and detected by conjugating the magnetic nanoparticles with recognition molecules. Afterwards, the elution solution (0.1-M sodium hydroxide) is added to displace the magnetic nanoparticles from the well surfaces into the solution. The detached magnetic nanoparticles reduce transverse relaxation time (T2) values of protons from the surrounding solution. A portable NMR relaxometer is used to measure the T2. Magnetic nanoparticles conjugated with streptavidin, monoclonal antibodies, and protein G were applied for the detection of biotinylated albumin, prostate-specific antigen, and IgG specific to tetanus toxoid (TT). The limit of detection of anti-TT IgG was 0.08-0.12 mIU/mL. The reproducibility of the assay was within the acceptable range (CV < 7.4%). The key novelty of the immunoassay is that the displacement of the nanoparticles from the solid support by the elution solution allows the advantages of the solid phase assay to be combined with the sensitive detection of the T2 changes in a volume of liquid.

Antibiotics in Bone Cements Used for Prosthesis Fixation: An Efficient Way to Prevent Staphylococcus aureus and Staphylococcus epidermidis Prosthetic Joint Infection.

Prosthetic joint infections (PJIs) are one of the most frequent reasons for arthroplasty revision. These infections are mostly associated with the formation of biofilm, notably by staphylococci, such as Staphylococcus aureus and Staphylococcus epidermidis. To minimize the rates of PJIs following primary or revision total joint arthroplasty, antibiotic-loaded bone cements (ALBCs) can be used for prosthesis fixation. However, its use is still debated. Indeed, various studies reported opposite results. In this context, we aimed to compare the prophylactic anti-biofilm activity of ALBCs loaded with two antibiotics with ALBC loaded with only one antibiotic. We compared commercial ready-to-use cements containing gentamicin alone, gentamicin plus vancomycin, and gentamicin plus clindamycin to plain cement (no antibiotic), investigating staphylococcal biofilm formation for 10 strains of S. aureus and S. epidermidis with specific resistance to gentamicin, vancomycin, or clindamycin. Firstly, we performed disk diffusion assays with the elution solutions. We reported that only the cement containing gentamicin and clindamycin was able to inhibit bacterial growth at Day 9, whereas cements with gentamicin only or gentamicin and vancomycin lost their antibacterial activity at Day 3. Then, we observed that all the tested ALBCs can inhibit biofilm formation by methicillin-susceptible staphylococci without other antibiotic resistance ability. Similar results were observed when we tested vancomycin-resistant or clindamycin-resistant staphylococci, with some strain-dependent significant increase of efficacy for the two antibiotic ALBCs when compared with gentamicin-loaded cement. However, adding vancomycin or clindamycin to gentamicin allows a better inhibition of biofilm formation when gentamicin-resistant strains were used. Our in vitro results suggest that using commercially available bone cements loaded with gentamicin plus vancomycin or clindamycin for prosthesis fixation can help in preventing staphylococcal PJIs following primary arthroplasties, non-septic revisions or septic revisions, especially to prevent PJIs caused by gentamicin-resistant staphylococci.

Sensitive fluorometric determination of gold in geological samples using fire assay pre-concentration coupled with microfluidic paper-based analytical device

The present study was conducted to develop a simple, low-cost and sensitive micro fluidic paper-based analytical device (µPAD) for analysis of gold in geological samples. This µPAD was fabricated employing eyeliner pencil method. It consists of different zones, including detection, pH adjustment, reaction and elution. We injected 29 µL elution solution in the center of µPAD, direct Au3+, after adjusting pH, to make it react with rhodamine B. The formation of RB-Au3+ complex led to quench of rhodamine B fluorescence. The image of each detection zone was captured using a cell phone camera under a UV lamp, and luminosity intensity was subsequently measured with Photoshop software. Various parameters were studied in order to achieve the most efficient performance of the paper sensor. Under the optimized condition, a wide linear range (1–40 mg L−1, R2 = 0.996) was obtained with detection limit of 0.15 mg L−1. Ultimately, the developed µPAD was applied for the determination of gold in ore samples. The obtained results were in good agreement with those obtained from standard method. The combination of fire assay and µPAD in the determination of gold in geological samples improved the detection limit based on enrichment factor of 75.

SEPARATION OF REBAUDIOSIDE A FROM Stevia rebaudiana EXTRACT USING LOW POLARITY RESIN AB-8

There are many methods to separate or purify the rebaudioside A compound from Stevia rebaudiana extract. However, the ion-exchange chromatography using macroporous resin is still the most popular among those methods. The separation of rebaudioside A from stevia crude extract by macroporous resin AB-8 was optimised in this adsorption separation study. This approach was applied to evaluate the influence of four factors such as the adsorption temperature, desorption time, elution solution ratio, and adsorption volume on rebaudioside A yield of the purified stevia extract. Theresults showed that the low polarity resin AB-8 is able to separate rebaudioside A from stevia extract with 0.601 in yield compared to the high polarity resin HPD 600 with 0.204 in yield used in Anvari and Khayati study. The best conditions for rebaudioside A separation by macroporous resin AB-8 were at 35°C of adsorption temperature, 30 min of desorption time, elution solution ratio 2:1, and 50 mL of adsorption volume.

Preparation and Characterization of scFv-Coupled Immunoaffinity Column for Purification of Fibrinolytic Enzyme from Bacillus subtilis Natto-89

Background: The focus of this study was to prepare and characterize the single-chain variable fragment antibody (scFv)-coupled immunoaffinity column for purification of subtilisin BRC. Methods: The scFv against subtilisin BRC was immobilized onto CNBr-activated Sepharose 4B. Adsorption isotherm for subtilisin BRC on scFv-BRC-coupled Sepharose 4B was obtained and calculated the maximum binding capacity. The extraction conditions, including eluting solution, the concentration of eluting solution and flow rate, were optimized. Under the optimized eluting conditions, the dynamic binding capacity of the immunoaffinity column was determined. Results: The scFv-BRC-coupled Sepharose 4B for immunoaffinity purification of subtilisin BRC was prepared. The coupling efficiency was about 78.4%, e.g. about 8 mg of scFv-BRC was covalently coupled to 1 g CNBr-activated Sepharose 4B. The maximum equilibrium binding capacity (qm) and dissociation constant (Kd) of the immunoaffinity column for subtilisin BRC were 3.01 mg/mL and 0.465 mg/mL, respectively. The immunoaffinity chromatography conditions were optimized and the subtilisin BRC was purified 3.29-fold with 55.6%. Conclusion: The subtilisin BRC was effectively purified with high purity using scFv-BRC-coupled Sepharose 4B and the dynamic binding capacity of the column was determined. These results suggested that scFv-BRC can be used as a ligand for affinity purification of subtilisin BRC.

Cu‐Mediated Radiofluorination of Aryl Pinacolboronate Esters: Alcohols as Solvents with Application to 6‐L‐[18F]FDOPA Synthesis

Cu‐mediated radiofluorination of arylboronic acid pinacol esters (ArylBPin) using Cu(OTf)2(Py)4 complex is a useful approach for the introduction of [18F]fluorine into non‐activated arenes and heteroarenes. Owing to the complexity of the mechanism and wide variation in substrate reactivity the choice of reaction conditions must be made individually for every precursor. Careful selection of phase‐transfer catalysts also plays a role, as Cu‐catalyst and ArylBPin precursors are known to be unstable in basic conditions. Using tetrabutylammonium triflate alcohol solution for elution of [18F]fluoride from ion‐exchange cartridge and employing same alcohol as a co‐solvent in the following labelling step, we have developed a general protocol resulting in &gt;80 % fluorination efficiency of ArylBPin precursors for 4‐[18F]fluoro‐D,L‐phenylalanine and 6‐L‐[18F]FDOPA. Radiofluorination in neat alcohol was particularly effective for the synthesis of 6‐L‐[18F]FDOPA, allowing for substantial increase of yield and decrease of synthesis time compared to current methods (activity yield 14.5 ± 0.5 %, 70 min synthesis time).

Evaluation of antibacterial and cytocompatible properties of multiple-ion releasing zinc-fluoride glass nanoparticles.

Zinc-fluoride glass nanoparticles (Zinc-F) release several ions, such as fluoride, zinc and calcium ions, through acid-base reactions. The aim of this study was to evaluate the antibacterial and cytotoxic properties of Zinc-F. Antibacterial tests showed that a Zinc-F eluting solution significantly reduced the turbidity and colony-forming units of Streptococcus mutans and Actinomyces naeslundii, compared to that of calcium-fluoroaluminosilicate glass nanoparticles without zinc ions. In live/dead staining, Zinc-F eluate significantly decreased green-stained bacterial cells, indicating live cells, compared with the control (no application). Human dentin coated with Zinc-F showed suppressed S. mutans and A. naeslundii biofilm formation. Additionally, Zinc-F eluate showed low cytotoxic effects in osteoblastic and fibroblastic cells. Therefore, our findings suggested that Zinc-F exhibits antibacterial and biocompatible properties through multiple-ion release.

Ion-exchange medium coated with abundant small zwitterions for the purification of soluble proteins

A new ion-exchange medium was prepared from magnetic particles of ∼1.0 µm through coating with small zwitterions and then functionalizing with ampholytic groups for the isoelectric point of ∼6.4 and denoted MSP-ZEWB. With Meyerozyma guilliermondii uricase (MGU) as the model of soluble proteins, the purification of a protein via ion-exchange was compared between MSP-ZEWB through elution in discontinuous mode and Toyopearl SP-650C as a classical ion-exchange medium through elution in continuous mode. MGU was adsorbed at pH 7.6 or 8.0 and eluted via competitive displacement by NaCl or electrostatic repulsions with an elution buffer at pH 10 to reverse the type of net charges of MGU. From MSP-ZEWB, MGU was eluted more rapidly with the elution percentages higher than those from Toyopearl SP-650C. For yielding a unit of MGU activity, MSP-ZEWB gave the elution solution volumes that were ∼50% of those obtained with Toyopearl SP-650C. The yields of MGU of the highest purity from MSP-ZEWB were higher than those from Toyopearl SP-650C, but the highest purification folds with both media were comparable. MSP-ZEWB regenerated for 16 times still showed the consistent purification efficacy. Therefore, the ion-exchange media bearing small zwitterion coats showed great promise for the purification of soluble proteins.

Highly sensitive determination of amanita toxins in biological samples using β-cyclodextrin collaborated molecularly imprinted polymers coupled with ultra-high performance liquid chromatography tandem mass spectrometry.

In this work, a β-cyclodextrin functional vinyl monomer was synthesized and the common moiety of five amanita toxins was used as the template for preparing molecularly imprinted polymers (MIPs). Chemical calculation was used to evaluate and describe the binding interactions between the template and the functional monomer. The preparation conditions were optimized and the resultant MIPs were characterized and employed as solid-phase extraction (SPE) sorbents. The SPE conditions including the amount of sorbent, extraction solution, and eluting solution were also optimized for the enrichment of the five toxins. Using an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), detection limits ranging from 0.34-0.42µg/L, 0.16-0.33µg/L, and 0.035-0.056µg/kg were achieved for the five toxins in serum, urine and liver samples, respectively. The proposed method was further applied to the determination of the amanita toxins in suspected samples and showed great potential in the diagnosis of mushroom poisoning.

Concentration of anionic surfactants from aqueous solutions on sorbents of various nature

The sorption isotherms of anionic surfactants (AS) from the aqueous solution on eight polar and nonpolar sorbents were obtained. The equilibrium characteristics of sorption were determined. The SV-50 activated carbon is recommended for the effective recovery of AS based on the values of maximum adsorption and concentration equilibrium constants. It has been established that, when the content of AS is much higher than the maximum permissible concentrations, adsorption proceeds via a monomolecular mechanism, and the onset of multilayer formation corresponds to half of the critical micelle concentration. A sorbent layer consisting of activated carbon SV-50 and ion exchange fiber FIBAN AK-22-1 is proposed for the concentration of AS from aqueous solutions. 0.1 M Solution of sodium sulfate is selected as a solution for elution. The concentration was carried out using sodium dodecyl sulfate as an example. An elution curve was obtained, and the characteristics of the concentration were calculated for AS. The proposed sorption extraction allows to reduce the detection limit of AS for the determination by ion-selective electrodes.

Facile preparation of metal organic framework-based laboratory semi-automatic micro-extraction syringe packed column for analysis of parabens in vegetable oil samples

A simple and efficient integrated, laboratory-made, semi-automatic microextraction packed in a syringe (Semi-automated MEPS) was first assembled and validated for the extraction of parabens in vegetable oils. Several representative metal–organic frameworks were prepared and used as sorbents for the direct extraction of analytes from diluted vegetable oils in a sealed syringe. The sorbent and adsorbed analytes were collected by an organic filter after syringe injection. After extraction, a simple device was used for the elution of analytes, which could greatly simplify the operation and reduce the total pretreatment time for oil matrix. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to separate and quantify parabens. Key factors affecting the extraction efficiency were investigated, such as volume ratio of oil sample to n-hexane, ultrasound extraction time, type and amount of sorbent, as well as the type, volume, and flow rate of elution solution. Under optimal conditions, the proposed method achieved low limits of quantification (1.97–4.57 ng/mL, S/N = 10) and good linearity (r > 0.9959) for all analytes recovered from various real samples. Relative standard deviations were calculated for all herbicides at two concentrations levels and were below 14.0%.

Metal-organic framework assisted matrix solid-phase dispersion microextraction of saponins using response surface methodology.

An efficient and simple metal-organic framework (MOF) assisted matrix solid-phase dispersion (MSPD) microextraction was developed for the extraction of the five saponins in P. ginseng leaves. The target analyses were detected by ultra high performance chromatography coupled with time-of-flight MS. Experimental conditions for MSPD microextraction were optimized by the Box-Behnken design of the response surface methodology. The optimal conditions were as follows: 20 mg adsorbent, 80% methanol-water solution for elution, 60 s grinding time, and the MOF-808 as the adsorbent. With the final optimized method, the calibration curves for five saponins showed good linearity (R2 > 0.998) within range of 0.01-100 μg/mL. In addition, analytical recoveries ranged from 87.04 to 103.78%, with the RSD below 5%. The limit of detection and LOQ range from 0.087 to 0.114 μg/mL and 0.292 to 0.379 μg/mL, respectively. Compared with the traditional extraction method and published methods, the newly MOF-assisted MSPD extract exhibited higher extraction efficiency, simpler operation, and provided a cleaner extract with low consumption of organic reagents that was applied for rapid evaluation and quality control of active compounds from plants.

Application of Fusarium sp. immobilized on multi-walled carbon nanotubes for solid-phase extraction and trace analysis of heavy metal cations

In the present work, for the first time, the filamentous fungus Fusarium sp. was utilized for devising a novel method for pre-concentration and determination of trace amounts of Pb(II), Cu(II), Cd(II), and Zn(II) ions, using a mini-column packed with Fusarium-coated multi-walled carbon nanotubes and inductively coupled plasma-optical emission spectrometry. Optimal analytical conditions including pH, ionic strength, elution solution, sample and eluent flow rates, and sample volume were determined. The detection limits were 0.39, 0.060, 0.021, and 0.025 ng mL−1 for Pb(II), Cu(II), Cd(II), and Zn(II) cations, respectively. This new method demonstrated a high performance for the analytes, and their adsorption was not affected by the different co-existing ions. The present procedure was validated by the analysis of standard reference materials, since the obtained data were in close agreement with reference values. Finally, this new procedure was successfully applied to analysis of heavy metal cations in natural food and water samples.

Simple, rapid, and cost-effective microextraction by the packed sorbent method for quantifying of urinary free catecholamines and metanephrines using liquid chromatography-tandem mass spectrometry and its application in clinical analysis.

Pheochromocytoma and paraganglioma (PPGL) are rare neuroendocrine tumors arising from adrenal and extra-adrenal chromaffin cells. They produce excessive amounts of catecholamines and their metabolites. A newly analytical procedure based on the semi-automated microextraction by packed sorbent (MEPS) technique, using a digitally controlled syringe (eVol) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS), was developed to quantify free urinary catecholamines and metanephrines. The important parameters affecting MEPS performance, namely the type of sorbent material (porous graphitized carbon (PGC), polar enhanced polymer (PEP), cation-exchange (CX) and C18), number of extraction cycles, and elution solvent system, were evaluated. The optimal experimental conditions involved the loading of sample mixture in seven extraction cycles through a C18 sorbent in a MEPS syringe, followed by using elution solutions (water/acetonitrile/formic acid, 95/4.75/0.25). The entire sample preparation took about 4min. Chromatographic separation was well achieved with an HSS PFP column using the gradient elution. The linearity range of the method was 0.167-33.4ng/mL for epinephrine, 0.650-130ng/mL for norepinephrine, 1.53-306ng/mL for dopamine, 1.34-268ng/mL for metanephrine, 3.43-686ng/mL for normetanephrine, and 1.33-265ng/mL for 3-methoxytyramine. The intra- and interassay precisions were ≤ 12.8%, and the respective accuracies were 88.4-112.0% and 89.0-109.5%. The carryover and sample stability without acidification were also investigated. Validation using clinical urine specimens showed that the proposed method had higher sensitivity compared with other urinary biochemical tests. The developed MEPS-LC-MS/MS method was simple, fast, and cost-effective; it helped to obtain information about multiple metabolites. It is applicable in routine clinical laboratories for the screening of PPGL. Graphical abstract.

The role of particle size and molar ratio of Ca2+/Na+ in the retention and remobilization of colloids in saturated porous media

The paper examined the effects of colloid size and molar ratio of Ca2+/Na+ on the retention and release of colloids under transients in ionic strength (IS). Column experiments were conducted to elucidate the transport behavior of three-sized polystyrene latex microspheres (300 nm, 500 nm, and 1000 nm) by varied molar ratios of Ca2+/Na+. Colloid concentration breakthrough curves showed the retention of colloids enhanced with the increase of molar ratio and the decrease of colloid size, which can be explained by Derjaguin–Landau–Verwey–Overbeek theory and the existence of nanoscale surface roughness. When the IS of the eluting solution was reduced from 10 to 1 mM, and when the eluting solution changed to ultrapure water, peak concentrations of colloid release were observed. Colloid recovery rates during the injection of ultrapure water were low, and substantial proportion of colloid still retained in the column even after the injection of ultrapure water, which suggested a stronger influence caused by the primary minimum compared with the secondary minimum on the retention of colloid. A modified numerical model considering the processes of Langmuirian dynamics blocking associated with a conservative tracer equation was successfully developed to simulate the transport process of all the experiments.

Sensitive determination of urinary muconic acid using magnetic dispersive-solid-phase extraction by magnetic amino-functionalised UiO-66

ABSTRACT In this study, a magnetic-dispersive solid-phase extraction (DSPE) was developed for the extraction and desorption of muconic acid from real urine sample. For this purpose, Fe3O4-SiO2-NH2@ UiO-66 was used as a Metal-Organic Framework (MOF) in the DSPE for extraction of the analyte of interest followed by High-Performance Liquid Chromatography (HPLC) analysis. The synthesised magnetic-MOF was characterised by XRD, VSM, FT-IR, and FE-SEM techniques. The experimental parameters influencing the magnetic-DSPE procedure, such as solution pH, initial sample volume, extraction and desorption time, type and volume of elution solution, and sorbent amount were examined. Under the optimal conditions, the UiO-magnetic DSPE-HPLC procedure provided a good limit of detection (0.001 µg mL−1), the limit of quantification (0.003 µg mL−1), wide linear dynamic range (0.003–25 µg mL−1) and high correlation coefficient (r2: 0.997). Also, the Fe3O4- SiO2- NH2@ UiO-66 exhibited an acceptable accuracy (2.0–4.0%), reproducibility (3.7–6.1%), and per cent recovery (96–98%) for the extraction and desorption of the desired analyte. It can be concluded that the proposed method was efficient for the extraction and analysis of muconic acid from real urine samples. Therefore, the Fe3O4- SiO2- NH2@ UiO-66 – DSPE-HPLC method can be used as a simple, fast, sensitive, reusable, and user-friendly extraction technique to determine trace levels of urinary muconic acid.

Soft-Templating of Sulfur and Iron Dual-Doped Mesoporous Carbons: Lead Adsorption in Mixtures.

Lead pollution in drinking water is one of the most common problems worldwide. In this research, sulfur and iron dual-doped mesoporous carbons are synthesized by soft-templating with sulfur content 4.4–6.1 atom% and iron content 7.8–9 atom%. Sulfur functionalities of the carbons are expected to enhance the affinity of the carbon toward lead whereas iron content is expected to separate the carbon from water owing to its magnetic properties. All the carbons were characterized by pore textural properties, x-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and energy dispersive x-ray (EDX). In order to study the Pb(II) removal efficiently of this carbon in competitive mode and to mimic the real-world use, one additional heavy-metal, including Cr(III), and four other commonly occurring metals—Na(I), K(I), Ca(II) and Fe (III)—are added with lead prior to adsorption experiments. It was observed that Pb(II) adsorption capacity of this carbon was not influenced by the presence of other metals. A highly elevated concentration of Na(I), K(I), Ca(II) and Fe(III) in the eluting solution compared to the initial dose suggested possible leaching of those metals from other salts as impurities, water source or even from the carbon itself, although the XPS analysis of the carbon confirmed negligible adsorption of those metals in carbon. From the equilibrium and kinetic data of adsorption, few parameters have been calculated, including distribution coefficient, diffusive time constant and pseudosecond order rate constant. The overall results suggest that these iron and sulfur dual-doped mesoporous carbons can serve as potential adsorbents for removal of lead from drinking water in the presence of other competing metals.