We used postembedding immunocytochemistry with an antiserum against BSA-conjugated GABA to study the inner plexiform layer of a cyprinid teleost, the roach. In this part of the retina, we observed a distinct banding pattern of GABA-positive material. There was a narrow unstained region separating the distal sublamina a from the proximal sublamina b; each sublamina was further subdivided into four ( a) and two ( b) sublayers of heavier staining, respectively. Using three-dimensional reconstruction of series of half-micron tangential sections, we were able to characterize a population of interstitial amacrine cells which contained GABA-like immunoreactive material. These cells had elliptical dendritic fields (area: about 0.04 mm 2) and conspicuous, thick processes (dia. 4–5 μm). In tangential sections, the dendrites of individual cells appeared to be in close contact, occasionally resulting in difficulties in defining the boundaries of a single dendritic field. Two subpopulations of these cells were observed, one in each sublamina. By comparison with a catalogue of Golgi impregnated amacrine cells and cells microinjected with Lucifer Yellow or HRP, the identity of this type of interstitial amacrine cell is established and its possible physiological properties discussed. Apart from this GABA positive type, a second population of interstitial amacrine cell was observed which did not show positive reaction to the GABA antiserum used.