To investigate the effect of exercise intensity on standing shooting performance and related technical variables in elite biathletes performing roller skiing and live shooting outdoors. Nineteen male biathletes performed two 5-shot series in the following order of exercise intensity: rest, low (%heart rate max 73 ± 4; blood lactate 1.5 ± 0.3 mmol·L-1), moderate (84 ± 3; 2.4 ± 0.6), 'race-pace' (90 ± 2; 4.5 ± 0.8), and 'final-lap' (i.e., near-maximal effort: 93 ± 3; 8.7 ± 1.4). Except for rest, each shooting series was preceded by 1 km roller ski skating on a competition track. Rifle movements and triggering were determined from marker-based motion capture and accelerometer data. The primary variables were shooting outcome (hit/miss) and distance from center (dC), determined from an electronic target, and barrel velocity. Mediation analyses for shooting outcome and dC were conducted with barrel velocity (mean over last 0.25 s before triggering) as mediator and intensity as predictor. Exercise intensity increased the likelihood of miss at 'race' (odds ratio (OR) 2.2, 95% CI 1.0-4.7) and 'final-lap' (OR 2.8, 95% CI 1.4-5.8) intensities compared to rest, with no meaningful differences between rest, low, and moderate intensities. Further, intensity affected dC (~32 ± 15 mm at rest, low, and moderate, 36 ± 20 mm at 'race', and 40 ± 23 mm at 'final-lap'; p < 0.001). Barrel velocity was a partial mediator of both shooting outcome and dC, explaining some, but not all, of the effect of intensity. Exercise intensity seems to have a clear negative effect on standing shooting performance in biathlon, which is partially explained by an increase in barrel velocity. Deteriorating effects were mainly seen at the two highest (race-like) intensities. Accordingly, for specificity reasons, more shooting practice should perhaps be performed at higher, competition-like exercise intensities than what is currently the norm.
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