Elevated Concentrations Research Articles

Factors related to elevated serum immunoglobulin G4 (IgG4) levels in a Japanese general population

BackgroundElevated serum immunoglobulin G4 (IgG4) concentrations are one of the characteristic findings in IgG4-related disease (IgG4-RD). This study investigated the frequency of elevated serum IgG4 levels and associated factors in a general Japanese population.MethodsSerum IgG4 concentrations were measured in 1,201 residents of Ishikawa prefecture who underwent general medical examinations. Factors associated with elevated serum IgG4 concentrations were assessed by logistic regression analysis. Participants with elevated serum IgG4 were subjected to secondary examinations.ResultsThe mean serum IgG4 concentration was 44 mg/dL, with 42 (3.5%) participants having elevated serum IgG4 levels. Age- and sex-adjusted logistic regression analyses showed that male sex, older age, and lower intake of lipids and polyunsaturated fatty acids and higher intake of carbohydrates in daily diet were associated with elevated serum IgG4 concentration. Subgroup analyses in men showed that older age, lower estimated glomerular filtration rates based on serum cystatin C (eGFR-cysC) levels, and higher hemoglobin A1c (HbA1c) levels were associated with elevated serum IgG4 concentration. Analyses in women showed that lower intake of lipids and fatty acids and higher intake of carbohydrates were significantly associated with elevated serum IgG4 concentration. One of the 15 participants who underwent secondary examinations was diagnosed with possible IgG4-related retroperitoneal fibrosis.ConclusionsElevated serum IgG4 levels in a Japanese general population were significantly associated with older age, male gender, and dietary intake of nutrients, with some of these factors identical to the epidemiological features of IgG4-RD.

Ameliorative Effect of Lycopene on Follicular Reserve Depletion, Oxidative Damage, Apoptosis Rate, and Hormonal Profile during Repeated Superovulations in Mice.

Superovulation is a crucial step in assisted reproductive technology that involves the administration of gonadotrophins. Repeated superovulations result in severe ovarian damage. The present study investigated the effect of in vivo administration of lycopene on ovarian damage induced by four successive cycles of superovulation. Superovulated mice were simultaneously administered intraperitoneally with saline (R4) or 5 mg/kg lycopene (R4-Lyc). The evaluated parameters were the count of different types of follicles, expression of ovarian antioxidant- and apoptosis-related genes, and serum concentrations of estradiol, progesterone, and inhibin-B. Increased numbers of healthy follicles and a decreased count of atretic follicles were observed in mice of the R4-Lyc group compared to those of the R4 group. Moreover, significantly higher mRNA levels of Sod3, Cat, and Nrf2 and lower mRNA levels of Keap1, Tnf, Nfkb, and Casp3, together with decreased H2O2 concentrations and increased total antioxidant capacity, were detected in the ovaries of lycopene-treated mice. Regarding serum reproductive hormones, elevated concentrations of estradiol, progesterone, and inhibin-B were evident in lycopene-administered mice. The present study reports a significant role of lycopene in alleviating the ovarian damage induced by multiple hormonal superstimulations, which could help to improve the outcomes of in vitro embryo production.

Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice.

Chlamydia muridarum (Cm) has reemerged as a moderately prevalent infectious agent in research mouse colonies. Despite its' experimental use, few studies evaluate Cm's effects on immunocompetent mice following its natural route of infection. A Cm field isolate was administered (orogastric gavage) to 8-week-old female BALB/cJ (C) mice. After confirming shedding (through 95d), these mice were cohoused with naive C57BL/6J (B6), C, and Swiss (J:ARC[S]) mice (n=28/strain) for 30 days. Cohoused mice (n=3-6 exposed and 1-6 control/strain) were evaluated 7, 14, 21, 63, 120, and 180 days post-cohousing (DPC) via hemograms, serum biochemistry analysis, fecal qPCR, histopathology, and Cm MOMP immunohistochemistry. Immunophenotyping was performed on spleen (B6, C, S; n=6/strain) and intestines (B6; n=6) at 14 and 63 DPC. Serum cytokine concentrations were measured (B6; n=6 exposed and 2 control) at 14 and 63 DPC. All B6 mice were shedding Cm by 3 through 180 DPI. One of 3 C and 1 of 6 S mice began shedding Cm at 3 and 14 DPC, respectively, with the remaining shedding thereafter. Clinical pathology was nonremarkable. Minimal-to-moderate enterotyphlocolitis and gastrointestinal associated lymphoid tissue (GALT) hyperplasia was observed in 15 and 47 of 76 Cm-infected mice, respectively. Cm antigen was frequently detected in GALT-associated surface intestinal epithelial cells. Splenic immunophenotyping revealed increased monocytes and shifts in T cell population subsets in all strains/timepoints. Gastrointestinal immunophenotyping (B6) revealed sustained increases in total inflammatory cells and elevated cytokine production in innate lymphoid cells and effector T cells (large intestine). Elevated concentrations of pro-inflammatory cytokines were detected in the serum (B6). Results demonstrate that while clinical disease was not appreciated, 3 commonly utilized strains of mice are susceptible to chronic enteric Cm infection which may alter various immune responses. Considering the widespread use of mice to model GI disease, institutions should consider excluding Cm from their colonies.

A comparative study of the efficacy of alginate lyases in the presence of metal ions elevated in the cystic fibrosis lung milieu

Pseudomonas aeruginosa, a common cause of morbidity in cystic fibrosis, chronically infects the patient's lungs by forming an alginate-containing biofilm. Alginate lyases are polysaccharide lyases that lyse alginate and are, therefore, potential biofilm-disruptive agents. However, cystic fibrosis sputum contains high levels of metals such as iron and zinc. The efficacy of alginate lyases under these conditions of elevated metal concentrations has not been categorically determined. Here, we have assessed the enzyme activity of two exolytic and five endolytic alginate lyases in the presence of metal ions (Fe2+, Zn2+, Mn2+, Mg2+, Ca2+, Ni2+, Cu2+) elevated in the cystic fibrosis lung milieu. Several of these alginate lyases exhibited increased activity in the presence of Ca2+, and the polysaccharide lyase family 7 members studied here exhibited decreased activity in the presence of Zn2+. The enzyme activity of the PL7 alginate lyases from Cellulophaga algicola (CaAly/CaFLDAly) and Vibrio sp. (VspAlyVI) was not affected in the presence of a mix of all the above-mentioned metal ions at the elevated concentrations found in the cystic fibrosis lung milieu. Specific alginate lyases might, therefore, retain the ability to degrade the alginate-containing Pseudomonas biofilm in the presence of metal ions elevated in the cystic fibrosis lung milieu.

Rare skin color changes in an acute pancreatitis patient undergoing maintenance hemodialysis

BackgroundSkin conditions are common in patients on maintenance hemodialysis and those with pancreatitis. However, there is a lack of research on dermatological issues in patients who have both hemodialysis and pancreatitis concurrently.Case presentationA 62-year-old male patient with a 4-year history of maintenance hemodialysis (MHD) presented with pain and was diagnosed with acute pancreatitis and gallbladder stones. Markedly elevated blood amylase, creatine kinase, and myoglobin were noted, alongside a purplish-red skin discoloration. Treatment included inhibition of digestive fluid secretion, anti-infection measures, blood purification, fasting, rehydration, and symptomatic care. Notably, continuous renal replacement therapy (CRRT) combined with hemoperfusion (HP) was employed. The patient’s dialysis effluent initially appeared red. Upon examination of the patient’s peripheral blood smear, red blood cell debris was not observed. The dialysis effluent (on Day 0) was analyzed, revealing no hemoglobin (0 g/L) but an elevated myoglobin concentration of 80.4 U/L. After the therapeutic intervention, the indicators, including the blood amylase, C-reactive protein, total bilirubin, creatine kinase, and myoglobin were improved. The patient experienced resolution of sternal and upper abdominal pain within two days. After four consecutive days of CRRT and HP treatment, the skin color returned to normal, alongside improved clarity of the dialysis effluent. Subsequently, the patient’s method of blood purification was reverted to conventional hemodialysis. On the eighth day of hospitalization, the patient resumed normal diet and was discharged.ConclusionsIn the case of the current patient with acute pancreatitis undergoing MHD, it is noteworthy to report the observation of a unique purplish-red skin discoloration. This phenomenon may be attributable to inflammation resulting from acute pancreatitis, and the retention of myoglobin within the body.

Analyzing Trends in Saharan Dust Concentration and Its Relation to Sargassum Blooms in the Eastern Caribbean

This study investigates the temporal trends and correlations between Saharan dust mass concentration densities (DMCD) and Sargassum concentrations (SCT) in the tropical North Atlantic. Average DMCD data for June, July, and August from 1980 to 2022, alongside SCT data for the same months from 2012 to 2022, were analyzed using Mann–Kendall tests for trends and lagged regression models to assess whether higher Saharan dust levels correlate with Sargassum outbreaks in the region. A comprehensive analysis reveals a significant upward trend in Saharan dust quantities over the study period, with the summer months of June, July, and August exhibiting consistent increases. Notably, 2018 and 2020 recorded the highest mean DMCD levels, with June showing the most significant increasing trend, peaking in 2019. These findings are consistent with previous studies indicating a continuous elevation in Saharan dust concentrations in the tropical atmosphere of the North Atlantic. Simultaneously, Sargassum concentrations also show a notable increasing trend, particularly in 2018, which experienced both peak SCT and elevated DMCD levels. Mann–Kendall tests confirm statistically significant upward trends in both Saharan dust and Sargassum concentrations. Simple linear regression and lagged regression analyses reveal positive correlations between DMCD and SCT, highlighting a temporal component with stronger associations observed in July and the overall June–July–August (JJA) period. These results underscore the potential contribution of elevated Saharan dust concentrations to the recent surge in Sargassum outbreaks in the tropical North Atlantic. Furthermore, the results from forward stepwise regression (FSR) models indicate that DMCD and chlorophyll (CHLO) are the most critical predictors of SCT for the summer months, while sea surface temperature (SST) was not a significant predictor. These findings emphasize the importance of monitoring Saharan dust and chlorophyll trends in the Eastern Caribbean, as both factors are essential for improving Sargassum modeling and prediction in the region. This study provides valuable insights into the climatic factors influencing marine ecosystems and highlights the need for integrated environmental monitoring to manage the impacts on coastal economies.

Elevated blood-ethanol concentration promotes reduction of aliphatic ketones (acetone and ethyl methyl ketone) to secondary alcohols along with slower oxidation to aliphatic diols.

Many people convicted for drunken driving suffer from an alcohol use disorder and some traffic offenders consume denatured alcohol for intoxication purposes. Venous blood samples from people arrested for driving under the influence of alcohol were analyzed in triplicate by headspace gas chromatography (HS-GC) using three different stationary phases. The gas chromatograms from this analysis sometimes showed peaks with retention times corresponding to acetone, ethyl methylketone (2-butanone), 2-propanol, and 2-butanol in addition to ethanol and the internal standard (1-propanol). Further investigations showed that these drink-driving suspects had consumed an industrial alcohol (T-Red) for intoxication purposes, which contained > 90% w/v ethanol, acetone (~ 2% w/v), 2-butanone (~ 5% w/v) as well as Bitrex to impart a bitter taste. In n = 75 blood samples from drinkers of T-Red, median concentrations of ethanol, acetone, 2-butanone, 2-propanol and 2-butanol were 2050mg/L (2.05g/L), 97mg/L, 48mg/L, 26mg/L and 20mg/L, respectively. In a separate GC analysis, 2,3-butanediol (median concentration 87mg/L) was identified in blood samples containing 2-butanone. When the redox state of the liver is shifted to a more reduced potential (excess NADH), which occurs during metabolism of ethanol, this favors the reduction of low molecular ketones into secondary alcohols via the alcohol dehydrogenase (ADH) pathway. Routine toxicological analysis of blood samples from apprehended drivers gave the opportunity to study metabolism of acetone and 2-butanone without having to administer these substances to human volunteers.

Verification and application of qPCR and viability-qPCR for Legionella monitoring in evaporative cooling systems complementing the conventional culture method

To date, in many countries the only legally valid method for evaporative cooling system (ECS) monitoring is the culture method. However, a duration of up to 14 days and a risk of underestimation of Legionella concentrations are seen as limitations of cultivation methods. Rapid cultivation-independent methods are an important step towards a more practicable monitoring of ECS to quickly control interventions if elevated concentrations of Legionella are found.Two commercial kits for quantitative polymerase chain reaction (qPCR) and viability-qPCR (v-qPCR) were studied, comprising sample filtration and DNA extraction. Cryopreserved Legionella pneumophila were established as calibration standard with intact (ILC) and total Legionella count (TLC) determined by flow cytometry before conducting spiking experiments in commercial mineral water and artificial process water. Final assessment was carried out using real ECS samples.Recovery and robustness ranged from 86 to 108 % for qPCR with a drop to 40–60 % for v-qPCR when compared to direct extraction, possibly attributable to cell damage during sample concentration. All methods including culture did perform well regarding linearity with R2 ≥ 0.95 for most trials. Detected concentrations in comparison to spiked Legionella counts differed with culture averaging 25 ± 7 % of spiked ILC and v-qPCR being closest to spiked concentrations with 65–144 %. In comparison, qPCR was several fold above spiked TLC concentrations. For real ECS samples Legionella spp. were detected in concentrations above 103 GU/100 mL by v-qPCR in 70–92 % of samples, depending on the kit used. Most of these samples were either culture-negative or not evaluable on agar plates.This study showed that a cryopreserved bacterial standard based examination is applicable and can be used for future v-qPCR verification. For assessment of differences in results between culture and v-qPCR/qPCR in ECS samples expert knowledge about the operating mode and used analytical methods is required. Guidelines addressing this issue could be a solution.

ADLM Guidance Document on the Measurement and Reporting of Lipids and Lipoproteins.

The accurate measurement of blood lipids and lipoproteins is crucial for the clinical management of atherosclerotic disease risk. Despite progress in standardization, there are still significant variations in pre-analytical requirements, methods, nomenclature, and reporting work flows. The guidance document aims to improve standardization of clinical lipid testing work flows. It provides recommendations for the components of the lipid panel, fasting requirements, reporting of results, and specific recommendations for non-high-density lipoprotein cholesterol (non-HDL-C), low-density lipoprotein cholesterol (LDL-C), lipoprotein(a) [Lp(a)], apolipoprotein B (apo B), point-of-care lipid testing, and LDL subfraction testing. Lipid panels should always report non-HDL-C and LDL-C calculations if possible. Fasting is not routinely required except in specific cases. Modern equations should be utilized for LDL-C calculation. These equations allow for LDL-C reporting at elevated concentrations of triglycerides and obviate the need for direct measured LDL-C in most cases.

Elevated osmol gaps in patients with alcoholic ketoacidosis.

The use of the osmol gap as a surrogate marker of toxic alcohol poisoning is common. Unfortunately, many patients with alcoholic ketoacidosis have elevated osmol gaps and are misdiagnosed with toxic alcohol poisoning. We aimed to characterize the range of osmol gaps in patients with alcoholic ketoacidosis. This was a retrospective poison center study. Data from 24 years were reviewed using the following case definition of alcoholic ketoacidosis: (1) documented alcohol use disorder; (2) presence of urine or serum ketones or an elevated blood beta-hydroxybutyrate concentration; (3) an anion gap ≥14 mmol/L. Potential cases of alcoholic ketoacidosis that failed to fulfill all three criteria were adjudicated by three toxicologists. Exclusion criteria included (1) detectable toxic alcohol concentration, (2) hemodialysis and/or multiple doses of fomepizole, (3) no osmol gap documented, (4) other diagnoses that lead to a metabolic acidosis. Demographics, pH, anion gap, lactate concentration, and osmol gap were extracted. Of 1,493 patients screened, 55 met criteria for alcoholic ketoacidosis. Sixty-four percent were male, and their median age was 52 years. The median osmol gap was 27 [IQR 18-36]. The largest anion gap was 57 mmol/L, and the lowest pH was 6.8. Forty-five (82%) of the patients with alcoholic ketoacidosis had osmol gaps >10; 38 (69%) had osmol gaps >20; 24 (44%) had osmol gaps >30; 11 (20%) had osmol gaps > 40. The large range of osmol gaps in patients with alcoholic ketoacidosis often reaches values associated with toxic alcohol poisoning. The study is limited by the potential for transcribing errors and the inability to identify the cause of the osmol gap. In this retrospective study, patients with alcoholic ketoacidosis had a median osmol gap of 26. Given that alcoholic ketoacidosis is easily and inexpensively treated, proper identification may prevent costly and invasive treatment directed at toxic alcohol poisoning.

The effect of pre-treatments on atrazine removal from source water by microbubble ozonation.

Ozone-based advanced oxidation processes (AOPs) have emerged a promising avenue for water treatment, offering effective removal of micropollutants. Recent research underscores the potential of ozone microbubbles to enhance ozone mass transfer during water treatment, particularly when combined with pre-treatment steps. This study aimed to evaluate the efficacy of three different combined processes (chlorine/KMnO4/PAC pre-treatment followed by ozonation) in removing atrazine, a common micropollutant from natural source water. Results revealed that all combined processes achieved higher atrazine removal rates compared to individual pre-treatment or ozonation methods. Notably, the highest atrazine removal rates were observed under alkaline pH conditions, with treatment outcomes influenced by oxidant dose and pH levels. Among the combined processes, chlorine pre-treatment followed by ozonation emerged as the most effective approach, achieving a removal rate of 59.7% that exceeded the sum of individual treatments. However, this treatment efficacy was affected by water quality parameters, particularly the presence of organic matter and elevated ammonia nitrogen concentration (> 0.5mg/L). This study highlights the potential for utilizing ozone micro/nanobubbles to enhance ozone mass transfer and offers valuable insights for optimizing the combined application of pre-treatment and ozonation strategies for efficient atrazine removal from natural water sources.

Engineering Corrosion Resistance in Magnesium Alloys for Biomedical Applications: A Synergy of Zn/Ca Atomic Ratio and Texture-Based Approach

Magnesium (Mg) and Magnesium-Zinc-Calcium alloys present a compelling option for biodegradable implant materials. Utilizing Vacuum Induction Casting, Mg–2.5Zn-xCa (with x = 0.3, 0.5, 0.9, 1.15 wt%) alloys were fabricated and subjected to hot-rolling for thermo-mechanical processing. The hot-rolled Mg–2.5Zn-0.3Ca alloy exhibits the lowest corrosion rate along with the highest basal texture. Increasing the Zn/Ca atomic ratio intensifies the basal texture and enhances corrosion resistance. Elevated Zn concentration improves corrosion resistance via Ca2Mg6Zn3 phase formation, while increased Ca content diminishes corrosion resistance due to the Mg2Ca phase. Advancement of this alloy is poised to extend Mg alloy use in innovative biomedical bone implants.

Realizing the Kinetic Origin of Hydrogen Evolution for Aqueous Zinc Metal Batteries

AbstractThe commercialization of zinc metal batteries (ZMBs) for large‐scale energy storage is hindered by challenges such as dendrite formation, the hydrogen evolution reaction (HER), and passivation/corrosion, which lead to poor stability of zinc metal anodes. HER is a primary contributor to this instability, and despite efforts to enhance ZMB cyclability, a significant knowledge gap remains regarding the origin of HER in these systems. Prior works, based primarily on theoretical calculations with minimal experimental support, suggest that HER originates from Zn2⁺‐solvated water. For the first time, by employing scanning electrochemical microscopy (SECM), and electrochemical mass spectrometry (ECMS), in real‐time the inherently intertwined nature of Zn electrodeposition and H₂ liberation is revealed, both exhibiting the same onset potential in voltammetry. The findings show that water molecules surrounding Zn2⁺ ions undergo reduction simultaneously during Zn2⁺ deposition. Additionally, ECMS conducted under chronopotentiometric/galvanostatic conditions at battery‐relevant current densities elucidates why elevated electrolyte concentrations enhance the prolonged cyclability of ZMBs. Understanding the origin of HER opens avenues for developing high‐performance, reliable aqueous ZMBs, addressing key challenges in their commercialization and advancing their technological capabilities.

Comprehensive analysis of benzothiazoles (BTHs), benzotriazoles (BTRs), and benzotriazole ultraviolet absorbers (BUVs) in the western South China Sea: Spatial distributions, migration tendencies and ecotoxicological relevance

Benzothiazoles (BTHs), benzotriazoles (BTRs), and benzotriazole ultraviolet absorbers (BUVs) have garnered significant attention owing to their persistent nature in the environment and adverse impacts on aquatic organisms. However, there remains a dearth of investigations and studies conducted in tropical marine environments. In this study, we undertook the inaugural distributional survey and ecotoxicological relevance of BTHs, BTRs, and BUVs in seawater and sediments of the western South China Sea (WSCS). Elevated concentrations of BTHs, BTRs, and BUVs in the seawater and suspended particulate matter (SPM) were primarily observed in the Pearl River Estuary (PRE) and the western region of the WSCS, attributed to terrestrial runoff and hydrodynamic processes. Moreover, the transport of these compounds at the seawater–SPM interface was influenced by both the intrinsic properties of the contaminants and temperature variations. Spatially, concentrations of BTHs, BTRs, and BUVs in surface sediments exhibited a diminishing trend with increasing distance from the coast to offshore areas, reflecting notable anthropogenic impacts. Concentration profiles of these compounds in sediment cores displayed a bottom-up increasing trend, with total organic carbon (TOC) identified as the primary determinant governing their accumulation within sediment cores in the WSCS. Terrestrial runoff inputs and atmospheric deposition as major contributors to the occurrence of BTHs, BTRs, and BUVs in the WSCS. Simultaneously, the study underscores the non-negligible moderate mixture risk quotient associated with BTHs, BTRs, and BUVs in the sediments.

Stage-Specific Plasma Metabolomic Profiles in Colorectal Cancer.

Background/Objectives: The objective of this study was to investigate the metabolomic profiles of patients with colorectal cancer (CRC) across various stages of the disease. Methods: The plasma samples were obtained from 255 subjects, including patients with CRC in stages I-IV, polyps, and controls. We employed capillary electrophoresis time-of-flight mass spectrometry and liquid chromatography triple quadrupole mass spectrometry to analyze hydrophilic metabolites comprehensively. The data were randomly divided into two groups, and consistent differences observed in both groups were analyzed. Results: Acetylated polyamines, such as N1-acetylspermine and N1, N12-diacetylspermine, consistently showed elevated concentrations in stage IV compared to stages I-III. Non-acetylated polyamines, including spermine and spermidine, exhibited increasing trends from polyp to stage IV. Other metabolites, such as histidine and o-acetylcarnitine, showed decreasing trends across stages. While acetylated polyamines have been reported as CRC detection markers, our findings suggest that they also possess diagnostic potential for distinguishing stage IV from other stages. Conclusions: This study showed stage-specific changes in metabolic profiles, including polyamines, of colorectal cancer.

Investigating the role of photochemistry and impact of regional and local contributions on gaseous pollutant concentrations (NO, NO2, O3, CO, and SO2) at urban and suburban sites

Gaseous pollutant concentrations were monitored for one year in urban (NO, NO2, O3, CO, and SO2) and suburban (NO, NO2, and O3) sites. Common characteristics were identified at both sites, such as higher concentrations during typical winter months for all pollutants except O3. Increased NO, NO2, CO and SO2 concentrations during wintertime were linked to residential heating emissions, with pairwise correlations showing CO as marker for the study site. O3 production was lower in wintertime but higher in summertime at both sites based on its seasonal cycle and the impact of sunlight. Furthermore, diurnal variations showed that traffic emissions during rush hours most profoundly affected CO and NO. Nevertheless, NOx were characteristically higher at the urban site based on the burdened environment, whereas O3 was higher at the suburban site due to the lower destruction rates. O3 was the dominant oxidant at both sites, with a linear regression between OX and NOx revealing a negative relationship. This observation suggested a dominant regional contribution to oxidant concentrations. In addition, abnormally high O3 concentrations in relation to the season were reported for the first time at the study sites. Elevated concentrations were measured in parallel with Sahara dust events indicating noteworthy atmospheric conditions that require further assessment. Other O3 burdened periods were driven by regional transport of polluted plumes from the northeast. Lastly, nocturnal increases in O3 were observed and associated with enhanced vertical mixing.

Trace organic contaminants in U.S. national park surface waters: Prevalence and ecological context

Surface water samples were collected from 264 sites across 46 U.S national parks during the period of 2009–2019. The number of sites within each park ranged from 1 to 31 and the number of samples collected within each park ranged from 1 to 201. Samples were analyzed for up to 340 trace organic contaminants (TrOCs), including pharmaceuticals, personal care products, pesticides, and various contaminants indicative of anthropogenic influence (e.g., fragrances, surfactants, flame retardants). A total of 155 TrOCs was detected in at least one sample with concentrations ranging from the reporting level of 10 ng/L (multiple contaminants) to 11,900 ng/L (p-cresol). Except for bisphenol A, DEET, theobromine, and gabapentin, TrOCs were detected in <20% of samples. Despite the relatively low detection frequencies, when TrOCs were detected, concentrations were similar to those reported from other regional or national studies. We compared detected concentrations to bioactivity concentrations and water quality benchmarks, when available, to identify occurrences of elevated concentrations and to estimate the potential for biological effects to aquatic biota. Elevated concentrations of 27 TrOCs, mostly pesticides, were detected throughout the study. To gain insight regarding potential sources, we related watershed characteristics (e.g., land cover, presence of point sources) to the number of TrOCs detected at each site. We found that the presence of wastewater treatment plants and the proportion of the watershed classified as agricultural land were the most influential variables for describing the number of pharmaceuticals and the number of pesticides present, respectively. This study represents the largest-scale study characterizing the presence and magnitude of TrOCs in U.S. national park surface waters, to date. These data provide a baseline that can be used to inform future monitoring within the parks and to assess changes in water quality.

Antibodies and complement are key drivers of thrombosis

Venous thromboembolism (VTE) is a common, deadly disease with an increasing incidence despite preventive efforts. Clinical observations have associated elevated antibody concentrations or antibody-based therapies with thrombotic events. However, how antibodies contribute to thrombosis is unknown. Here, we show that reduced blood flow enabled immunoglobulin M (IgM) to bind to FcμR and the polymeric immunoglobulin receptor (pIgR), initiating endothelial activation and platelet recruitment. Subsequently, the procoagulant surface of activated platelets accommodated antigen- and FcγR-independent IgG deposition. This leads to classical complement activation, setting in motion a prothrombotic vicious circle. Key elements of this mechanism were present in humans in the setting of venous stasis as well as in the dysregulated immunothrombosis of COVID-19. This antibody-driven thrombosis can be prevented by pharmacologically targeting complement. Hence, our results uncover antibodies as previously unrecognized central regulators of thrombosis. These findings carry relevance for therapeutic application of antibodies and open innovative avenues to target thrombosis without compromising hemostasis.

Size sieving and electrostatic exclusion synergetic strategy for high efficiency recovery of superbase-derived ionic liquids via nanofiltration from the spinning process

Superbase-derived ionic liquids (SILs) as the promising green solvents for cellulose spinning process should be urgently and deeply evaluated with respect to their recyclability. Herein, size sieving and electrostatic exclusion synergetic strategy was adopted for recycling four SILs ([DBUH][CH3CH2OCH2COO], [DBUH][CH3OCH2COO], [DBNH][CH3CH2OCH2COO], and [DBNH][CH3OCH2COO]) from aqueous solutions by the nanofiltration membranes (NF270 and NF90) at the first time. NF270 with larger pore and more negative charge was conducive to recycle SIL on account of a higher volume flux and the remarkably high IL rejection (＞95 %) compared to NF90. Rising the pressure and temperature played the positive role in the permeate flux of the membranes, whereas the elevated SIL concentration markedly decreased the electrostatic exclusion, leading to a decline in the recovery efficiency. Benefiting from the size sieving effect, the [DBNH][CH3OCH2COO] with small cation and anion structure could be recovered efficiently due to the high energy gap and big IL aggregates. Conversely, the strong electrostatic attraction was appeared between [DBUH][CH3CH2OCH2COO] (low energy gap and high positive charge density) and the membranes, leading to a decline in recovery efficiency. Furthermore, the combination of nanofiltration and evaporation to recover SIL from spinning wastewater effectively reduced the total recovery cost (1.51 $/Kg) in comparison with the evaporation only. Insight gained from this work suggested a high-efficiency and economical approach could be used in the recovery of SILs with small cations and anions size via large pore nanofiltration membranes during the industrialized cellulose spinning process.

Utilizing bypass cement dust in the production of radiation shielding bismuth borate glass

Using the melting-quench technique, a new method for removing bypass cement dust (BCD) was developed in this study using an active environmental glassy system with the chemical formula (65–x)B2O3–5Li2O–5CdO–25BCD–xBi2O3, where x varied from 0 to 30 mol%. A glass density of 3.137 g/cm³ is attained without bismuth, while a density of 5.345 g/cm³ is obtained with 30 mol% Bi2O3. The unit peaks BO3 and BO4 in the FTIR spectra signify the presence of the BLCDBi-series structure. Elevated Bi2O3 concentrations augment BO4 units. Defects influence the optical absorbance of glass within the wavelength range of 190 to 1000 nm. These defects in the glass network are created by silicon electrons, silicon hole centers, and BCD as well as Bi3+ or Bi6+ after the addition of bismuth oxide to the glassy system. Eg values were found to decrease as Bi2O3 concentration increased, whereas EU and n values exhibited the opposite behavior. A gamma spectroscopy system and the Phy-X/PSD software are used to calculate the linear attenuation coefficient (LAC), mass attenuation coefficient (MAC), half-value layer (HVL), tenth-value layer (TVL), effective atomic number (Zeff), and effective electron density (Neff) of samples. In addition, the Phy-X/PSD software demonstrated good agreement between experimental and theoretical interpretations. Overall, the results showed that the BLCDBi-30 sample is suitable for use in radiation protection.