Elephantopus Scaber Research Articles

Callus induction and secondary metabolite profile from <i>Elephantopus scaber</i> L.

Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol. Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.

Uji Aktivitas Antibakteri Ekstrak Etanol Daun Tapak Liman (Elephantopus Scaber L ) Terhadap Bakteri Shigella Dysenteriae Dengan Metode Difusi Cakra,

Dysentery is an inflammation of the intestine caused by shigella bacteria which can lead to physiological disorders and becomes a health problem that needs to be considered. Liman Tread Leaves ( Elephantopus scaber ) contains high flavonoid compounds, phenols and saponins which are reported to have antioxidants, antibacterial, antiviral and anti-inflammation. This study aims to find out the activity of ethanol extract of liman tread leaves ( Elephantopus Scaber L ) as an antibacterial and to find out the minimum microbial death rate value for Shigella spp bacteria. This research uses the True Experimental Post-Test Only Control Group Design where Liman Tread Leaves are extracted by maceration method and placed on MHA media overgrown by bacteria. Based on the statistics test results using regression test, the t-count value is 4,336 > 2,178 showing an influence of Liman Tread Leaf extract with a bland zone of 65.4%.

Three new sesquiterpene lactones from the whole plants of Elephantopus scaber

Three new sesquiterpene lactones, named scabertopinolides H − J (1 − 3), along with four known ones, desacylisodeoxyelephantopin 2-methylbutyrate (4), iso-17,19-dihydrodeoxyelephantopin (5), scabertopinolide D (6) and (2R,6R,7R,8S)-8-tigloyloxy-1(10),4(5),11(13)-germacratrien-2,15,6,12-diolide (7) were isolated from the whole plants of Elephantopus scaber. Their structures were elucidated by extensive analysis of spectroscopic data (including IR, UV, HRESIMS, 1 D and 2 D NMR) and single-crystal X-ray. These isolated compounds showed effective anti-inflammatory effects on LPS-stimulated RAW 264.7 cells with IC50 values of 6.27 ± 0.18 to 18.31 ± 1.38 μM.

Extract of Elephantopus scaber as Therapy for Insulin Resistance by Decreasing Damage of Heart and Liver in STZ-Na Induced Diabetic Rats (Rattus novergicus)

Extract of Elephantopus scaber as Therapy for Insulin Resistance by Decreasing Damage of Heart and Liver in STZ-Na Induced Diabetic Rats (Rattus novergicus)

Targeted isolation of cytotoxic germacranolide sesquiterpenes from Elephantopus scaber L. using small molecule accurate recognition technology

Small molecule accurate recognition technology (SMART) is an emerging method for the rapid structural prediction of major constituents from crude extracts and fractions. In the present study, a targeted isolation of an Elephantopus scaber extract by SMART resulted in the obtention of 15 new (1–15) and five known germacranolide sesquiterpenes (16–20). Their structures were assigned by extensively analyzing HRESIMS, NMR, X-ray crystallographic analyses, modified Mosher’s method results, and quantum chemical calculate electronic circular dichroism (ECD) spectra. All germacranolide sesquiterpenes were screened to determine their inhibitory effects with two hepatoma cell lines (HepG2 and Hep3B), and compounds 14, 16, 18, 19 and 20 showed significant cytotoxic activities against the HepG2 (IC50, 3.3–9.9 μM) and Hep3B (IC50, 4.5–8.6 μM) cell lines. Further study suggested that 18 can induce the apoptosis of hepatoma cells via mitochondrial dysfunction.

Isodeoxyelephantopin Inactivates Thioredoxin Reductase 1 and Activates ROS-Mediated JNK Signaling Pathway to Exacerbate Cisplatin Effectiveness in Human Colon Cancer Cells.

Colon cancer is one of the leading causes of cancer-related death in the world. The development of new drugs and therapeutic strategies for patients with colon cancer are urgently needed. Isodeoxyelephantopin (ESI), a sesquiterpene lactone isolated from the medicinal plant Elephantopus scaber L., has been reported to exert antitumor effects on several cancer cells. However, the molecular mechanisms underlying the action of ESI is still elusive. In the present study, we found that ESI potently suppressed cell proliferation in human colon cancer cells. Furthermore, our results showed that ESI treatment markedly increased cellular reactive oxygen species (ROS) levels by inhibiting thioredoxin reductase 1 (TrxR1) activity, which leads to activation of the JNK signaling pathway and eventually cell death in HCT116 and RKO cells. Importantly, we found that ESI markedly enhanced cisplatin-induced cytotoxicity in HCT116 and RKO cells. Combination of ESI and cisplatin significantly increased the production of ROS, resulting in activation of the JNK signaling pathway in HCT116 and RKO cells. In vivo, we found that ESI combined with cisplatin significantly suppressed tumor growth in HCT116 xenograft models. Together, our study provide a preclinical proof-of-concept for ESI as a potential strategy for colon cancer treatment.

Deoxyelephantopin, a novel naturally occurring phytochemical impairs growth, induces G2/M arrest, ROS-mediated apoptosis and modulates lncRNA expression against uterine leiomyoma

Deoxyelephantopin (DOE), a phytochemical, extracted and purified from Elephantopus scaber, has been shown to exhibit antitumor activities. Objective of the present study was to investigate anti-tumor and apoptosis-inducing properties of DOE against uterine leiomyoma (UL) and to explore their molecular mechanisms. Primary cell cultures from fresh UL tissue were established and maintained up to 12 passages. The cells exhibited continuous proliferation with 24 -h doubling time until 12 passages and was then subjected to molecular characterization. The growth inhibitory effect of DOE on UL cells was confirmed by colony formation, cellular senescence, AO/PI and DAPI staining. Fluorescent-activated cell sorting (FACS) assay for apoptosis and cell cycle arrest analysis revealed that DOE significantly (p < 0.05) inhibited the UL cell proliferation via cell cycle arrest at G2/M phase and induced apoptosis via ROS production by lowering mitochondrial membrane potential. Subsequently, the DOE induced ROS was markedly attenuated by co-treatment of N-Acetyl-Cysteine (NAC). Our quantitative RT-PCR and western blot results showed up-regulation of Bax, Caspase-3 and down-regulation of Bcl2, P53, αSMA, COL4A2, VEGF, PCNA, Cyclin B1 and oncogenic lncRNAs (H19, HOTAIR, BANCR and ROR) in DOE treated UL cells which further strengthen our findings. In conclusion, DOE inhibits growth of UL cells via cell cycle arrest at G2/M phase, induces ROS-dependent caspase-3-mediated mitochondrial intrinsic apoptotic pathway and down-regulation of oncogenic lncRNA in UL cells. Our findings suggest that DOE deserves for further systematic investigation in the uterine leiomyoma animal model as a novel apoptosis inducer for potential applications in the prevention or treatment of uterine leiomyoma.

The hypoglicemic effects of tapak liman (Elephantopus scaber L) plant extract on albino rat (Rattus novergicus) models of diabetes mellitus

Background: Diabetes mellitus (DM) is among the diseases with high morbidity and mortality The pathogenesis of DM involves pancreatic s-cell damage or insulin sensitivity disorder that result in hyperglycemia. Tapak liman (Elephantopus scaber L) is known to have high flavonoid content. Flavonoids are antioxidants that play a role in reducing pancreatic s-cell damage or damage to other tissues, which potentially reduce blood glucose levels. Objective: To determine the hypoglycemic effects of tapak liman using the DM rat models. Methods: Twenty-eight Wistar albino rats (Rattus novergicus) were divided into four groups: the normal control group (KKn), not induced by streptozotocin-nicotinamide (STZ-NA) intraperitoneal; negative control group (KK-), induced by STZ-NA; treatment group 1 (KP1), induced by STZ-NA and 150 mg/kg body weight of tapak liman plant extract; and the treatment group 2 (KP2), induced by STZ-NA and 300 mg/kg body weight of tapak liman. Blood glucose levels were measured on the 7th day after STZ-NA induction and the 28th day after the administration of tapak liman plant extract. The results were analyzed for statistical significance. Results: There was a significant decrease in blood glucose levels in KP1 and KP2 (p= 0.05). The decrease in blood glucose in KP1 had not reached normal levels compared to KP2. Conclusion: Administration of tapak liman plant extract at a dose of 300 mg/kg body weight in DM rat models reduced blood glucose levels to normal.

Germacranolides from Elephantopus scaber L. and their cytotoxic activities

Seven undescribed germacranolides, named as scabertopinolide A-G were obtained from whole herbs of Elephantopus scaber L. The determination of their structures was conducted via comprehensive spectroscopic analyses combined with experimental electronic circular dichroism (ECD) spectroscopic data and quantum mechanical ECD calculations. The absolute configuration of scabertopinolide A was determined by X-ray crystallography data analysis. The cytotoxicity of all compounds was evaluated against three human cancer cell lines HepG2, Hep3B (human hepatocellular carcinoma cell lines), and MCF-7 (human breast adenocarcinoma cell line). Scabertopinolide G exhibited the most significant cytotoxic activities against the three cancer cell lines with IC50 values between 7.0 and 10.3 μM. Furthermore, flow cytometry analysis has suggested that scabertopinolide G may cause death of cancer cells through apoptosis induction.

Simultaneous RP-HPLC Quantification of Four Phenolics in Elephantopus scaber L. and their in vitro Pharmacological Validation

Simultaneous RP-HPLC Quantification of Four Phenolics in Elephantopus scaber L. and their in vitro Pharmacological Validation

Effectiveness of herbs containing Curcuma xanthorrhiza, Elephantopus scaber L and Amaranthus tricolor L in iron deficiency anemia patients

Objective: this study aimed to evaluate the effectiveness of herbs containing Curcuma xanthorrhiza, Elephantopus scaber L and Amaranthus tricolor L compared with iron supplement in Iron Deficiency Anemia (IDA) subjects.&#x0D; Materials and Methods: a total of 128 subjects with IDA were recruited and randomly assigned to one of the two groups: Anemia herbs (AH) (n=64) and Iron supplement (IS) (n=64). The AH group received herbs containing Curcuma xanthorrhiza, Elephantopus scaber L and Amaranthus tricolor L two times daily and the IS group received Hufabion capsul for 4 weeks. Outcome measures were assesed 4 weeks after intervention. The outcomes of this study were hemoglobin (Hb), mean corpuscular volume (MCV), serum iron (SI), total iron banding capacity (TIBC) and quality of life (QOL) by using short form-36 (SF-36). Ethical Committee of NIHRD approval was obtained for this study.&#x0D; Results: A significant QOL effect in score of SF-36 of AH group was found after 4 weeks intervention (p&lt;0,05). In contrast, no significant effect were found for the value of Hb, MCV , SI and TIBC (p&gt;0,05) in the AH group. In the IS group, there were significant differences for value of Hb, MCV, SI, TIBC and SF-36 scores (p=0,000). Significant group effects were found for the value of Hb, MCV, SI and TIBC when comparing the IS group to AH group. There were also no significant differences between groups for QOL scores after 4 weeks intervention.&#x0D; Conclusion: Herbs containing Curcuma xanthorrhiza, Elephantopus scaber L and Amaranthus tricolor L proved to be an effective complementary therapy by improve QOL in the IDA patients.

Elephantopinolide A-P, germacrane-type sesquiterpene lactones from Elephantopus scaber induce apoptosis, autophagy and G2/M phase arrest in hepatocellular carcinoma cells

Chromatographic purification of Elephantopus scaber led to 16 new germacrane-type sesquiterpene lactones (1–16), named elephantopinolide A-P, along with a known analogue (17). Their structures were confirmed by comprehensive spectroscopic analyses, single-crystal X-ray diffraction, and comparison between the experimental and calculated ECD spectra. Their hepatocellular inhibition activities against Hep3B and HepG2 cells were screened by MTT assay, and the structure-activity relationships were examined. The results revealed that 10 (IC50 value of 2.83 μM and 1.98 μM) is more potent than sorafenib. The underlying mechanism study demonstrated that 10 could markedly induce apoptosis accompanied by increased ROS production and decreased mitochondrial membrane potential, resulting in the autophagy and G2/M phase cell arrest in Hep3B and HepG2 cells. Furthermore, signal pathways including MAPKs and AKT may play important roles in 10-induced hepatocellular carcinoma cells death.

Isodeoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber Linn., inhibits pro-inflammatory mediators’ production through both NF-κB and AP-1 pathways in LPS-activated macrophages

Isodeoxyelephantopin (IDET) has been identified as an anti-tumor natural constituent whose anti-tumor activity and mechanism have been widely investigated. Since the occurrence and development of cancer usually accompany with inflammation, and tumor signaling shares many components with inflammation signaling, the agents with anti-tumor activity are likely to possess anti-inflammation potential. Thus, the current study aims to demonstrate the anti-inflammatory activity along with the underlying mechanism of IDET in lipopolysaccharide (LPS)-primed macrophages. By using Griess method and ELISA, we found that in both bone marrow derived macrophages and alveolar macrophage cell line, IDET, at relatively low concentrations (0.75, 1.5 and 3 μM), could inhibit LPS-induced expression of various pro-inflammatory mediators including nitric oxide (NO) generated by inducible nitric oxide synthase (iNOS), interleukin (IL)-6, monocyte chemotactic protein-1 (MCP-1) and IL-1β. Meanwhile, in activated MH-S cells, the inhibitory action of IDET on mRNA expression levels of these cytokines was also detected using qPCR. Mechanistically, the effects of IDET on two key inflammatory signalings, nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) pathways, were determined in LPS-activated MH-S cells by reporter gene along with western blot assays. On the one hand, IDET suppressed NF-κB signaling via down-regulating phosphorylation and degradation of inhibitor of NF-κB (IκB)-α and the subsequent p65 translocation. On the other hand, IDET dampened AP-1 signaling through attenuating phosphorylation of both c-jun N-terminal kinase 1/2 (JNK1/2) and extracellular signal regulated kinase 1/2 (ERK1/2). Our study indicates that IDET might be a promising constituent from the anti-inflammatory herb Elephantopus scaber Linn. in mitigating inflammatory conditions, especially respiratory inflammation.

New phenolic acids from the whole herb of Elephantopus scaber Linn. and their anti-inflammatory activity

Two new phenolic acids, ethyl 3,3′,4,4′-tetrahydroxy-δ-truxinate (1), 3-O-p-coumaroyl-4-O-caffeoyl quinic acid methyl ester (2), together with three known compounds (3–5) were isolated from the whole plant of Elephantopus scaber Linn. The structures of the new compounds were elucidated using detailed spectroscopic analysis. Compound 3 was obtained and given its NMR data for the first time. All isolates were evaluated for their anti-inflammatory activity via inhibiting the production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells, and 1, 4 and 5 showed a moderate inhibition with IC50 values ranging from 11.85 to 20.62 μM.

Đa dạng hình thái và giải phẫu thực vật của hai loài cúc chỉ thiên Elephantopus mollis H.B.K. và Elephantopus scaber L. tại Bảy Núi, tỉnh An Giang

Elephantopus mollis and Elephantopus scaber were collected from Bay Nui region (An Giang province) for three years (2016-2019). Their morphological characteristics, anatomical structures and medicinal powder were analyzed and compared with purpose of supplementing data to Elephantopus genus in Vietnam. The results showed that the morphological similarities of these two species occur at the sapling stage. At the mature stage, E. scaber has underground stem, purple flowers, and E. mollis has aerial stem, white flowers. In addition, these two species are able to be distinguished based on the size, thickness, tips shape or color of all leaves. The anatomical characteristics and the structure of the medicinal powder also showed differences in number and arrangement of xylem, and in covering trichomes of the two species at two stages (sapling and adult stages). Some by-products such as calcium oxalate crystals, essential oil-secreting cells and inulin crystals were found in the vegetative organs of E. mollis and E. scaber collected from An Giang province.

ANTIPROLIFERATIVE ACTIVITY OF ELEPHANTOPUS SCABER MEDIATED SILVER NANOPARTICLES AGAINST MCF-7, A-549, SCC-40, AND COLO-205 HUMAN CANCER CELL LINES

Objective: To unearth the applications of nanotechnology in medicine has become imperative with all the advancements in the technique. In the current study, we have attempted to exploit the anticancer ability of the green synthesized silver nanoparticles (AgNPs).&#x0D; Methods: The AgNPs were synthesized using 60% methanol (H-MeOH) Elephantopus scaber leaf extract, characterized, and discussed priorly. The effect of AgNPs was studied on the human breast (MCF-7), lung (A-549), oral (SCC-40), and colon (COLO-205) cancer cell lines through sulforhodamine B assay. We also carried out the synergistic activity with standard drug adriamycin (ADR).&#x0D; Results: According to the results obtained, AgNPs showed good antiproliferative activity with GI50 &lt;10 μg/ml on MCF-7, A-549, and SCC-40 cell lines when compared with the standard drug ADR. However, for COLO-205 cell line, the impact was 17.4 μg/ml and thus the treatment was less effective.&#x0D; Conclusion: The synergistic effect of AgNPs+ADR was even better for all the four cell lines than that of the AgNPs alone.

Ethanol extract of Elephantopus scaber Linn. Attenuates inflammatory response via the inhibition of NF-κB signaling by dampening p65-DNA binding activity in lipopolysaccharide-activated macrophages.

Elephantopus scaber Linn. (E.scaber) is a widely-used traditional herb whose use has been documented for various inflammatory diseases such as fever, sore throat, dysentery, carbuncle and so on. However, the effect and mechanism of E.scaber in LPS-activated macrophages remain unclear. This study aims to investigate the anti-inflammatory mechanism of the ethanol extract of E.scaber (ESE) in lipopolysaccharide (LPS)-induced inflammatory models. Griess reagent was used to determine NO production, and the levels of TNF-α, IL-6, MCP-1 and IL-1β were determined by ELISA kits. The molecular mechanism research was performed by RT-PCR, Western blot, and electrophoretic mobility shift assay (EMSA). LPS-induced endotoxemia mouse model was used for evaluating the in vivo anti-inflammatory action of ESE. ESE suppressed LPS-induced iNOS, TNF-α, IL-6, MCP-1 and IL-1β transcription as well as supernatant NO, TNF-α, IL-6, MCP-1 and IL-1β production in macrophages. Although ESE inhibited NF-κB activation, it did not affect the IκBα phosphorylation and degradation and the NF-κB p65 nuclear translocation. The result of EMSA revealed that ESE inhibited the NF-κB p65-DNA binding activity. Additionally, ESE also decreased the proinflammatory cytokines in serum and peritoneal lavage fluid of LPS-induced endotoxemic mice. ESE has a potently anti-inflammatory effect through inhibiting the NF-κB p65-DNA binding activity in LPS-activated macrophages.

Deoxyelephantopin decreases the release of inflammatory cytokines in macrophage associated with attenuation of aerobic glycolysis via modulation of PKM2

Growing evidence suggests that activated immune cells undergo metabolic reprogramming in the regulation of the innate inflammatory response. Remarkably, macrophages activated by lipopolysaccharide (LPS) induce a switch from oxidative phosphorylation to aerobic glycolysis, and consequently results in release of proinflammatory cytokines. Pyruvate Kinase M2 (PKM2) plays a vital role in the process of macrophage activation, promoting the inflammatory response in sepsis and septic shock. Deoxyelephantopin (DET), a naturally occurring sesquiterpene lactone from Elephantopus scaber, has been shown to counteracts inflammation during fulminant hepatitis progression, but the underlying mechanism remains unclear. Here, we studied the function of the DET on macrophage activation and investigated the anti-inflammatory effects of DET associated with interfering with glycolysis in macrophage. Our results first demonstrated that DET attenuates LPS-induced interleukin-1β (IL-1β) and high-mobility group box 1 (HMGB1) release in vitro and in vivo and protected mice against lethal endotoxemia. Furthermore, DET decreased the expression of pyruvate dehydrogenase kinase 1 (PDK1), glucose transporter 1(GLUT1), lactate dehydrogenase A (LDHA), and reduced lactate production dose-dependently in macrophages. Moreover, we further revealed that DET attenuates aerobic glycolysis in macrophages associated with regulating the nuclear localization of PKM2. Our results provided a novel mechanism for DET suppression of macrophages activation implicated in anti-inflammatory therapy.

Analysis Activity of Elephantopus scaber Leaves Extract Against Quantitative Changes of Lymphocytes Cells in BALB/c Mice After Induction of DMBA and Estrogen

The carcinogenic compounds such as DMBA known for having the ability to promote the DNA damage that affects the growth regulator genes. Therefore, uncontrolled cell growth will occur and change the immune system. Elephantopus scaber extract is considered as an alternative way of handling breast cancer since it capable to affect the work of the immune system. Hence, this study aims to determine the effect of E. scaber extract on quantitative changes of CD4 + , CD8 + T cells and proinflammatory cytokines CD4 + IFN-γ + and CD4 + TNF-α + after induction of DMBA and estrogen. In vivo experiments were carried out by using 5-6 weeks old of female mice BALB/c strain. The extract of E. scaber leaf powder was obtained from Material Medica Batu, Malang. The experimental animals were divided into five groups such as negative control group (K-), positive control group (K+), mice treated with E. scaber extract for a week (group 1), mice treated with E. scaber extract for 2 weeks (group 2), and mice treated with E. scaber extract for 3 weeks (group 3). Induction of DMBA and estrogen was conducted by injection of DMBA (7.12 dimetylbenz (α) anthracene) 0.56 mg.kgBW -1 and estradiol hormone 0.0504 mg.kgBW -1 . Mice were treated with E. scaber extract with a dose of 50 mg.kgBW -1 . Lymphocytes were isolated from spleen and observed the CD4 + , CD8 + T cells and proinflammatory cytokines CD4 + IFN-γ + and CD4 + TNF-α + T cells through flow cytometry analysis. One-way ANOVA (p <0.05) and SPSS were used to analyze this data. The results showed that the induction of DMBA and estradiol hormone affected mice fur loss. It also decreased the immune system by lowering the number of CD4 and CD8 T cells. However, E. scaber extracts increased the number of pro-inflammatory cytokines TNF-α. Furthermore, the DMBA and estradiol induction also promoted the number of CD4 + IFN-γ + in the first week, but decreased the number of CD4 + IFN-γ + at weeks 2 and 3, then increase the number of CD4 + IFN-γ + . So, the treatment of E. scaber extracts demonstrated the ability to restore the homeostasis of the immune system in the first and second weeks. Keywords: CD4 + , CD8 + , DMBA, estrogen, E.scaber, IFN-γ + , TNF-α +

The Effect of Ethanol Extract of Elephantopus scaber Linn in Decreasing Blood Uric Acid Levels of Hyperuricemic Male Rats

Background: Gout causes attacks of pain and swelling in one or more joints and control of serum uric acid level has been used as one of the therapeutic methods for gout. Inhibition method of xanthine oxidase (XO) activity which can oxidize hypoxanthine to uric acid has been commonly used to decrease serum uric acid level. On the other hand, Elephantopus scaber Linn leaf has been used for the treatment of rheumatoid arthritis as folk medicine by some people in Indonesia.&#x0D; Aims: This study was to determine the hypouricemic effect of ethanol extract of Elephantopus scaber leaf by in vivo study in caffeine (PO)-induced hyperuricemic male rats.&#x0D; Methodology: The E. scaber leaf was obtained from Research Institute for Spices and Medicinal Plants, Bagor, Indonesia. Preparation of E. scaber leaf extract was done by cold maceration extraction technique using ethanol 70%. Male rats (Sprague-Dawley) were induced by using caffeine with dose 27 mg/200 g b.w until the levels in the blood of male rats become hyperuricemic. Equipment (Easy Touch) was used by detecting the level of uric acid in a rat blood. Equipment (Easy Touch) was used by detecting the level of uric acid in a rat blood. Rats were divided into 6 groups, as a positive control was used allopurinol 36 mg/200 g b.w, test preparation were used 3 doses, i.e, 175 mg/200 g b.w, 350 mg/200 g b.w and 700 mg/200 b.w which suspended in Na-CMC 0.5%.&#x0D; Results: The results from a decrease in blood uric acid levels in rats, on the 15th days showed that a dose of 350 mg / 200 g b.w gave decrease in the highest percentage ie 43%. Statistical analysis on the 15th day showed that all of the test preparation groups of E. scaber had effects decrease uric acid in blood serum of rats and significantly different from negative controls (p ≤ 0.05). The dose of 350 mg/200 g b.w of E. scaber had the same effect with a dose of 36 mg/200 g b.w allopurinol in reducing uric acid in experimental rats.&#x0D; Conclusions: E. scaber is a plant that is quite potential to be used in the treatment of gout.