Structural Elements Research Articles

Bio-Based Epoxy Vitrimers with Excellent Properties of Self-Healing, Recyclability, and Welding

The development of more recyclable materials is a key requirement for a transition towards a more circular economy. Thanks to exchange reactions, vitrimer, an attractive alternative for recyclable materials, is an innovative class of polymers that is able to change its topology without decreasing its connectivity. In this work, a bisphenol compound (VP) was prepared from saturated cardanol, i.e., 3−pentadecylphenol and vanillyl alcohol. Then, VP was epoxidized to obtain epoxide (VPGE). Finally, VPGE and citric acid (CA) were polymerized in the presence of catalyst TBD to prepare a fully bio−based vitrimer based on transesterification. The results from differential scanning calorimetry (DSC) showed that the VPGE/CA system could be crosslinked at around 163 °C. The cardanol−derived vitrimers had good network rearrangement properties. Meanwhile, because of the dynamic structural elements in the network, the material was endowed with excellent self−healing, welding, and recyclability.

Optimized analysis and reinforcement design model for RC/SCC box-section bridges using developed advanced design framework

In recent years, there has been a significant rise in the construction of reinforced concrete (RC) and steel-concrete composite (SCC) box-section bridges. However, design codes do not provide specific design methods for the RC plates of such bridges where the plates experience both intricate forces and moments. The current design approaches are primarily based on conventional one-dimensional beam element theory, which neglects the complex spatial force characteristics of the structural elements, potentially leading to unsafe design. Therefore, it is imperative to address this issue. To bridge this gap, a comprehensive analysis and reinforcement design procedure named as the developed advanced design framework (DADF) is proposed in this study. The spatial analysis of bridges was performed using a refined numerical model, called the spatial grid model (SGM), to consider the structural spatial effects with limited model scales. Subsequently, the internal force corresponding matrix (IFCM) was implemented through Python scripts to determine the most critical internal force combination for an RC plate element. Additionally, the three-layered sandwich model (T-LSM) was employed with limit analysis to automatically design reinforcement for all elements, serving as a practical tool for design. This research demonstrates that the implementation of the DADF can significantly reduce steel reinforcement in the RC plates of such bridges by up to 48 %, and 23 % in total, showcasing the efficiency of the developed framework. Overall, the findings emphasize that the proposed DADF offers a robust procedure for steel reinforcement design of RC/SCC box-section bridges.

Prepositional case constructions with the preposition <i>ot</i> (English <i>from</i>) in the poetry of F. I. Tyutchev

Function words, for instance, simple prepositions, have significant potential for meaning making and are an important element of the grammatical structure of the text, which, along with the lexical one, is involved in creating the imagery of an artwork. The insufficient knowledge of the semantic charge of simple prepositions in F. I. Tyutchev’s poetic texts determined the relevance of this study. The research aims to develop a typology of the prepositional-case constructions with the preposition ot (English from) and determine their place in the authors’ dictionary of the poet’s vocabulary. To analyse the 118 uses of phrases with the preposition ot obtained from the "Concordance of the Complete Poems of F. I. Tyutchev. A–Z", we employed the descriptive method in conjunction with observation techniques; generalisation of the material; componential analysis (identification of semantic components of the meaning of the preposition ot); distributional analysis that enabled us to identify the combinability of the preposition ot with other linguistic units. When describing the meanings of prepositional-case constructions with the preposition ot, we considered the morphological and semantic features of the head and dependent words included in the phrases with case government. The study identified and analysed the object, adverbial (locative, temporal, causal, of manner), and attributive relations realised in prepositional-case constructions. Additionally, we specified additional meanings acquired by prepositional combinations in poetic texts. The research shows that constructions with the preposition ot form a fragment of the authors’ dictionary of F. I. Tyutchev’s language.

Практики хозяйствования народов Республики Хакасия в современных условиях

In today’s limited conditions of the Russian rural labour market, the role of economic activity of the population as an additional or even the main means of subsistence gains an increasing importance. This problem is relevant for people who live primarily in rural areas. The specifics of the existing management of local ethnic communities are established in the culture and presented in the form of ethno-economic structures. Today, their preservation and development is considered to be an opportunity for the growth of regional economies. To what extent have peoples preserved elements of the traditional economic structure at the present stage? The given study was conducted in the Republic of Khakassia, where the Khakass (the titular ethnic group of the republic), the Shors (small-numbered indigenous people), and the Russians (the ethnic majority in the region) live. The article compares the economic practices of these ethnic communities. The results of the study showed that indigenous peoples retain their orientation towards traditional types of economic activities. Khakassians are actively involved in livestock breeding. Compared to Russians and Shors, they have a more numerous and more varied livestock farmstead. Shors are focused primarily on wild capture activities; for many, it is the main type of employment. Russians are less involved in economic activities, but in villages where the Khakass population predominates, they keep a lot of livestock. They are less inclined to consider economic activity as a significant source. Khakassians are oriented towards economic activity; they mainly associate their life strategies with the village. Shors try to preserve traditional crafts, which are the most accessible source of subsistence for them in taiga conditions. In the current conditions, it is difficult to talk about the significant influence of the ethno-economic structures of indigenous peoples on the development of the economy of Khakassia, since these ethnic groups constitute a numerical minority on the territory of the republic.

Magnetic Nitrogen-Doped Fe<sub>3</sub>C@ c Catalysts for Efficient Activation of Peroxymonosulfate for Degradation of Organic Pollutants

Recoverable and stable nanocatalysts are essential for peroxymonosulfate - based advanced oxidation processes (AOPs) in wastewater purification treatment. In this paper, Fe3C nanorods @ nitrogen-doped carbon composites (N-Fe3C@C) with core-shell architecture were fabricated by the co-precipitation and calcination methods, and characterized and analyzed in terms of their crystal structure, microscopic morphology, and surface chemical elements. In addition, N-Fe3C@C-4 degraded 85.36% of tetracycline in 10 min under PMS, which was much higher than the catalytic ability of Fe3O4 (42.03% in 10 min). Both the active radical trapping and EPR experiments verified that 1O2 played a key role for degradation of organic dyes in PMS system. The investigation on the degradation mechanism revealed that the presence of the carbon layer facilitated to adsorb TC, accelerate free radical generation and promote the redox cycle of Fe2+/Fe3+ in the nanocatalyst. This study offers novel ideas for multifunctional catalysts for advanced wastewater purification treatment.

Structural Catalytic Core of the Members of the Superfamily of Acid Proteases

The superfamily of acid proteases has two catalytic aspartates for proteolysis of their peptide substrates. Here, we show a minimal structural scaffold, the structural catalytic core (SCC), which is conserved within each family of acid proteases, but varies between families, and thus can serve as a structural marker of four individual protease families. The SCC is a dimer of several structural blocks, such as the DD-link, D-loop, and G-loop, around two catalytic aspartates in each protease subunit or an individual chain. A dimer made of two (D-loop + DD-link) structural elements makes a DD-zone, and the D-loop + G-loop combination makes a psi-loop. These structural markers are useful for protein comparison, structure identification, protein family separation, and protein engineering.

High-Throughput Sequencing Reveals New Viroid Species in Opuntia in Mexico

In the main cactus pear (Opuntia ficus-indica)-producing region in the State of Mexico, fruit production occupies the largest cultivated area with 15,800 ha, while 900 ha are cultivated for edible young Opuntia pads (“nopalitos”) which are consumed as vegetables. Two composite samples consisting of cladodes of plants for fruit production (n = 6) and another of “nopalitos” (n = 6) showing virus-like symptoms were collected. Both sample sets were subjected to high-throughput sequencing (HTS) to identify the viruses and viroids. The HTS results were verified using RT-PCR and Sanger sequencing. Subsequently, 86 samples including cladodes from “nopalitos”, plants for fruit production, xoconostles, and some wild Opuntia were analyzed via RT-PCR with specific primers for the viruses and viroids previously detected via HTS. Three viruses were discovered [Opuntia virus 2 (OV2), cactus carlavirus 1 (CCV-1), and Opuntia potexvirus A (OPV-A)], along with a previously reported viroid [Opuntia viroid 1 (OVd-1)]. Additionally, two new viroids were identified, provisionally named the Mexican opuntia viroid (MOVd, genus Pospiviroid) and Opuntia viroid 2 (OVd-2, genus Apscaviroid). A phylogenetic analysis, pairwise identity comparison, and conserved structural elements analysis confirmed the classification of these two viroids as new species within the Pospiviroidae family. This is the first report of a pospiviroid and two apscaviroids infecting cactus pears in the world. Overall, this study enhances our understanding of the virome associated with cactus pears in Mexico.

Investigating the Catalytic Site of Human 15-lipoxygenase-1 via Marine Natural Products.

Human 15-lipoxygenase-1 (15-LOX-1) is a key enzyme that possesses an important role in (neuro)inflammatory diseases. The pocket of the enzyme plays the role of a chiral catalyst, and therefore chirality could be an important component for the design of effective enzyme inhibitors. To advance our knowledge on this concept, we developed a library of the identified chiral 15-LOX-1 inhibitors and applied cheminformatic tools. Our analysis highlighted specific structural elements, which we integrated them in small molecules, and employed them as "smart" tools to effectively navigate the chemical space of previously unexplored regions. To this purpose, we utilized the marine derived natural product phosphoeleganin (PE) among with a small library of synthetic fragment derivatives, including a certain degree of stereochemical diversity. Enzyme inhibition/kinetic and molecular modelling studies has been performed in order to characterize structurally novel PE-based inhibitors, which proved to present a different type of inhibition with low micromolar potency, according to their structural features. We demonstrate that different warheads work as anchor, and either guide specific stereochemistry, or causing a time-depended inhibition. Finally, we prove that the positioning of the chiral substituents or/and the favorable stereochemistry can be crucial, as it can lead from active to completely inactive compounds.

Stereoscopic Microscopy of Snow Crystals

Abstract Snow crystals (snowflakes) are composed of an enormous diversity of shapes that have attracted significant scientific attention over the past decades. Under the microscope, these natural structures are frequently characterized by high complexity, the detailed exploration of which requires the use of enhanced magnifications. In the work presented here, an insight into the microcosm of snow crystals is presented by application of specific light microscopy techniques. Micrographs were produced by means of a low-temperature (LT) device that prevented premature melting of the structures. Additionally, single crystals were imaged as to a stereoscopic visualization allowing the detailed spatial investigation of specific structural elements.

An Improved Diabatization Scheme for Computing the Electronic Circular Dichroism of Proteins.

We advance the quality of first-principles calculations of protein electronic circular dichroism (CD) through an amelioration of a key deficiency of a previous procedure that involved diabatization of electronic states on the amide chromophore (to obtain interamide couplings) in a β-strand conformation of a diamide. This yields substantially improved calculated far-ultraviolet (far-UV) electronic circular dichroism (CD) spectra for β-sheet conformations. The interamide couplings from the diabatization procedure for 13 secondary structural elements (13 diamide structures) are applied to compute the CD spectra for seven example proteins: myoglobin (α helix), jacalin (β strand), concanavalin A (β type I), elastase (β type II), papain (α + β), 310-helix bundle (310-helix) and snow flea antifreeze protein (polyproline). In all cases, except concanavalin A and papain, the CD spectra computed using the interamide couplings from the diabatization procedure yield improved agreement with experiment with respect to previous first-principles calculations.

Genome-Wide Identification and Characterization of Sucrose Metabolism Genes Involved in Actinidia rufa and Their Expression Profiling during the Fruit Developmental Stages

Soluble sugars, as an indispensable source of energy, play crucial roles in plant growth and development. However, to date, scant information about the sucrose metabolism-related gene families is available in kiwifruit (Actinidia rufa Planch). Here, we systematically identified the members of various gene families encoding crucial enzymes or transporters involved in sucrose metabolism, re-analyzed their expression patterns under different fruit development stages, and determined the regulatory mechanism involving key transcription factors. A total of sixty-two genes from six gene families (thirty-one ArINVs, two ArSPPs, four ArSPSs, nine ArSUSs, six ArSUCs/SUTs, and ten ArSWEETs) were identified in the A. rufa genome. The characterization of these members, including gene structure, motifs, conserved domains, and cis-acting elements, were analyzed. Phylogenetic analysis revealed that these gene families could be categorized into six distinct subgroups. These six gene families have undergone strong purifying selection during domestication. In addition, expression analysis of the 62 genes indicated that differential expression patterns are highly regulated by developmental processes. Specifically, we identified 11 transcription factors that were rigorously correlated (r > 0.98) with key gene expression profiles. This represents a comprehensive analysis of the genes involved in sucrose metabolism in kiwifruit, which provides useful information for further functional analysis of these genes.

Seismic retrofitting optimization model using fiber-reinforced polymer jacketing and NSGA-III

Fiber-reinforced polymer (FRP) is widely used for retrofitting structural elements due to its easy application. However, establishing a retrofit strategy is challenging due to conflicting objectives, such as cost and performance level, requiring optimization for effective decision-making. This study proposes a many-objective optimization model for seismic retrofitting using FRP and the Non-dominated Sorting Genetic Algorithm (NSGA)-III. The model's efficacy was demonstrated through two numerical examples: a reinforced concrete building retrofitted with FRP jackets and a masonry-infilled reinforced concrete building retrofitted with FRP bracings. Each example included three objective functions and multiple constraints. Nonlinear static pushover analysis provided optimal strategies to enhance base shear and energy dissipation while minimizing costs and retrofitting locations. Among the Pareto-optimal solutions, the optimal solution with the minimum Euclidean distance was selected. NSGA-III offered a wider distribution and more Pareto-optimal solutions compared to NSGA-II, demonstrating its potential in addressing many-objective problems related to retrofit decision-making.

Geological controls of mineralization occurrences in the Egyptian Eastern Desert using advanced integration of remote sensing and magnetic data

This study presents a comprehensive analysis of mineralization exploration in the Egyptian Eastern Desert (ED), one of the most sought-after areas for those interested in mining industry, by integrating Landsat-9 images and geophysical magnetic data. Employing advanced techniques like Principal Component (PC) analysis, Minimum Noise Fraction (MNf) transform, and Band-Ratio (B-Ratio), the research focuses on mapping lithological units, hydrothermal alteration regions, and structural elements. Composite images derived from specific PC, and MNf bands, and B-Ratio exhibit superior lithological unit identification. The findings emphasize that there are significant variations in the types of rocks extend from the southern to the northern parts of the ED. Hydrothermal alteration mapping, guided by B-Ratio results, aids qualitative lithological discrimination. A novel false color composite image optimizes Landsat-9 B-Ratios, enhancing rock unit discrimination. Correlation analyses reveal associations between mineralization types and major lithological units, while exploration of the magnetic anomaly map highlights its role in correlating mineralization sites. Structural features, analyzed through Center for Exploration-Targeting Grid-Analysis (CET-GA) and Center for Exploration-Targeting Porphyry-Analysis (CET-GA) with Tilt Derivative of RTP (TDR) techniques, contribute to a robust association between regions with medium to high structural density and porphyry intrusions and mineralization. The study significantly supports the advanced exploration geoscience, providing insights into the geological structures and dynamics governing mineralization in the Egyptian ED.

Impact of Digital Technologies on Implementing Individual Elements of the Tax Structure

Impact of Digital Technologies on Implementing Individual Elements of the Tax Structure

Earthquake Performance Analysis of a Masonry School Building's Retrofitted State by the Equivalent Frame Method

Nonlinear analyses of masonry structures are frequently used in both engineering practice and academic studies. Due to the dominant nonlinear behaviour of masonry structures, complex and extensive finite element models are required to obtain accurate analysis results. While masonry walls are usually modelled using fine-meshed shells or solid elements in such structures, high computing power in modelling, analyzing, and post-processing results is necessary for the analyses of large structures. In recent years, the equivalent frame method, as a solution to this problem has been developed and presented in the literature. In this study, the equivalent frame method is used in a masonry structure modelling, and the axial force-bending relationship is represented by force-based fiber elements. The multi-linear load-deformation relationship reflects the shear behaviour of the walls. Within the scope of the study, an existing masonry school building is modelled using the equivalent frame elements with OpenSees software. Seismic performance analyses are done considering the existing and retrofitted states of the structure, and the results are discussed in a comparative manner.

Hydrogen-Bonding and Hydrophobic Interaction Networks as Structural Determinants of Microbial Rhodopsin Function.

Microbial pump rhodopsins are highly versatile light-driven membrane proteins that couple protein conformational dynamics with ion translocation across the cell membranes. Understanding how microbial pump rhodopsins use specific amino acid residues at key functional sites to control ion selectivity and ion pumping direction is of general interest for membrane transporters, and could guide site-directed mutagenesis for optogenetics applications. To enable direct comparisons between proteins with different sequences we implement, for the first time, a unique numbering scheme for the microbial pump rhodopsin residues, NS-mrho. We use NS-mrho to show that distinct microbial pump rhodopsins typically have hydrogen-bond networks that are less conserved than anticipated from the amino acid residue conservation, whereas their hydrophobic interaction networks are largely conserved. To illustrate the role of the hydrogen-bond networks as structural elements that determine the functionality of microbial pump rhodopsins, we performed experiments, atomic-level simulations, and hydrogen bond network analyses on GR, the outward proton pump from Gloeobacter violaceus, and KR2, the outward sodium pump from Krokinobacter eikastus. The experiments indicate that multiple mutations that recover KR2 amino acid residues in GR not only fail to convert it into a sodium pump, but completely inactivate GR by abolishing photoisomerization of the retinal chromophore. This observation could be attributed to the drastically altered hydrogen-bond interaction network identified with simulations and network analyses. Taken together, our findings suggest that functional specificity could be encoded in the collective hydrogen-bond network of microbial pump rhodopsins.

Role of WAVE3 as an of actin binding protein in the pathology of triple negative breast cancer.

Breast cancer, a prevalent global health concern, has sparked extensive research efforts, particularly focusing on triple negative breast cancer (TNBC), a subtype lacking estrogen receptor (ER), progesterone receptor, and epidermal growth factor receptor. TNBC's aggressive nature and resistance to hormone-based therapies heightens the risk of tumor progression and recurrence. Actin-binding proteins, specifically WAVE3 from the Wiskott-Aldrich syndrome protein (WASP) family, have emerged as major drivers in understanding TNBC biology. This review delves into the intricate molecular makeup of TNBC, shedding light on actin's fundamental role in cellular processes. Actin, a structural element in the cytoskeleton, regulates various cellular pathways essential for homeostasis. Its dynamic nature enables functions such as cell migration, motility, intracellular transport, cell division, and signal transduction. Actin-binding proteins, including WAVE3, play pivotal roles in these processes. WAVE3, a member of the WASP family, remains the focus of this review due to its potential involvement in TNBC progression. While actin-binding proteins are studied for their roles in healthy cellular cycles, their significance in TNBC remains underexplored. This review aims to discuss WAVE3's impact on TNBC, exploring its molecular makeup, functions, and significance in tumor progression. The intricate structure of WAVE3, featuring elements like the verprolin-cofilin-acidic domain and regulatory elements, plays a crucial role in regulating actin dynamics. Dysregulation of WAVE3 in TNBC has been linked to enhanced cell migration, invasion, extracellular matrix remodeling, epithelial-mesenchymal transition, tumor proliferation, and therapeutic resistance. Understanding the role of actin-binding proteins in cancer biology has potential clinical implications, making them potential prognostic biomarkers and promising therapeutic targets. The review emphasizes the need for further research into actin-binding proteins' clinical applications, diagnostic value, and therapeutic interventions. In conclusion, this comprehensive review explores the complex interplay between actin and actin-binding proteins, with special emphasis on WAVE3, in the context of TNBC. By unraveling the molecular intricacies, structural characteristics, and functional significance, the review paves the way for future research directions, clinical applications, and potential therapeutic strategies in the challenging landscape of TNBC.

TECTONIC AND NON-TECTONIC MESO-STRUCTURES IN THE LISAN FORMATION AND EQUIVALENT RECENT SEDIMENTS OF THE JORDAN RIFT VALLEY-CHARACTERIZATION AND FORMATION MECHANISMS

In this study, the structural elements portrayed in the recent sediments of the Dead Sea, which became exposed at its shores due to the drop in its level are described, and the mechanisms leading to their formation are elaborated. These structures include Plumes and density inversions, subaquatic gliding, seismites, undulations and wavy structures, flexures, and overpressure structures. The formation of these structures is found to be the result of different triggering factors such as Earthquakes, tectonic activity, instability of slopes, density inversion, pore fluids overpressure resulting from gas production by biochemical processes within the sediments (sulfur bacteria reduction of sulfates and oxidation of organic matter (petroleum residues)), submarine groundwater discharge, and volume changes associated with the transformation of gypsum to basinite and anhydrite, and the reversal of that transformation. Each of these triggering factors can produce more than one type of structure, depending on the prevailing composition of the sediments, its layering attitudes, grain-size distribution, porosities, and differential pressure situations within the sediment packages. The main finding of the study is the clarification of the role of gas production within the sediments as a result of sulfur bacteria activity and of the volume change processes associated with the transformation of gypsum to anhydrite and the reversal of that transformation in the formation of the mesostructures of the Dead Sea sediments.

Exploring Damage Patterns in CFRP Reinforcements: Insights from Simulation and Experimentation.

Carbon Fiber Reinforced Polymers (CFRP) have become increasingly significant in real-world applications due to their superior strength-to-weight ratio, corrosion resistance, and high stiffness. These properties make CFRP an ideal material for reinforcing concrete structures, particularly in scenarios where weight reduction is crucial, such as in bridges and high-rise buildings. The transformative potential of CFRP lies in its ability to enhance the durability and load-bearing capacity of concrete structures while minimizing maintenance costs and extending the lifespan of the infrastructure. This research explores the impact of reinforcing structural elements with advanced composite materials on the strength and durability of concrete and reinforced concrete structures. By integrating Carbon Fiber Reinforced Polymer (CFRP) reinforcements, we subjected both rectangular and T-section concrete beams to comprehensive three-point bending tests, revealing a substantial increase in flexural strength by 45% and crack resistance due to CFRP reinforcement. The study revealed that CFRP reinforcement increased the flexural strength of concrete beams by 45% and improved crack resistance significantly. Additionally, the load-bearing capacity of the beams was enhanced by 40% compared to unreinforced specimens. These improvements were validated through finite element simulations, which showed a close alignment with the experimental data. Furthermore, an innovative simulation study was conducted using a finely tuned finite element numerical model within the Abaqus calculation code. This model accurately replicated the laboratory specimens in terms of shape, dimensions, and loading conditions. The simulation results not only validated the experimental observations but also provided deeper insights into the stress distribution and failure mechanisms of the reinforced beams. Novel aspects of this study include the identification of specific failure patterns unique to CFRP-reinforced beams and the introduction of an enhanced interaction model that more accurately reflects the composite behavior under load. In CFRP-reinforced beams, specific failure patterns were identified, including flexural cracks in the tension zone and debonding of the CFRP sheets. These patterns indicate the points of maximum stress concentration and potential weaknesses in the reinforcement strategy. The study revealed that while CFRP significantly improves the overall strength and stiffness, careful attention must be given to the bonding process and the quality of the adhesive used to ensure optimal performance. These findings contribute significantly to the understanding of material interactions and structural performance, offering new pathways for the design and optimization of composite-reinforced concrete structures. This research underscores the transformative potential of composite materials in elevating the structural integrity and longevity of concrete infrastructures.

The Cerebral Aqueduct Compliance: A Simple Morphometric Model.

This work aimed to identify different configurations of the adytum of the cerebral aqueduct suggesting its safe neuroendoscopic navigation. This concept is intimately connected to the physiological aqueductal dilatability or compliance, which is relatively ignored in the literature. A better knowledge of the extent of physiological aqueductal dilatability might better define the ideal diameter and safer features of dedicated flexible endoscopes. The study includes 45 patients operated on using a flexible scope with a 3.9-mm diameter, where the structural elements of the adytum of the cerebral aqueduct are clearly visible. Patients were grouped according to the pathology (colloid cyst/normal anatomy, intraventricular hemorrhage, tetraventricular obstructive hydrocephalus, normal pressure hydrocephalus, and distal membranous aqueductal stenosis). A simple geometrical scheme was applied to the endoscopic anatomy of the aqueductal adytum in relation to the posterior commissure to measure its pathologic deformations. Eventual damages to the aqueduct walls caused by the endoscope were also reported. Proceeding from normal anatomy to hydrocephalic condition, the ratio between the commissure and the aqueductal access area progressively decreases, while the vertex angle increases. Interestingly, the entity of the ependymal damages due to the passage of the endoscope correlates with such measures. The cerebral aqueduct, excluding atrophic processes, is provided with a certain degree of dilatability, which we estimate to be around a diameter of 4 mm. This represents the maximum size for a flexible neuroendoscope for a safe aqueductal neuronavigation. The schematic model of the aqueductal adytum as a triangle defines 3 different aqueductal patterns and can be helpful when an intraoperative decision on whether to navigate the aqueduct must be taken.