Bioaccumulation of trace elements in aquatic environments can be influenced by local environmental conditions such as temperature fluctuations, pH levels, sediment composition, dissolved organic matter content, and the presence of other chemical substances. We analyzed the differences in trace elements accumulation (S, Cl, K, Ca, Ti, Cr, Mn, Fe, Cu, Zn, Rb, Sr, Mo, Ba, and Pb) between two trophic guilds-scrapers (Ephemeroptera) and predators (Plecoptera)-of freshwater benthic macroinvertebrates collected from mountain streams in Kazakhstan and Slovakia. Trace elements in dried insect bodies were analyzed using an X-ray spectrometer, and physicochemical parameters of stream water were investigated at each sampling site. Our results showed significant differences in Fe, Ti, and Sr levels in predators from Kazakhstan and Cu levels in predators from Slovakia. Despite some trace elements showing higher concentrations in one group over another, the overall differences between regions were more pronounced. Principal component analysis (PCA) revealed that the primary factors influencing trace elements variability were associated with environmental conditions such as temperature, oxygen levels, and total dissolved solids (TDS). PCA components indicated a higher load of trace elements in the warmer, less oxygenated streams, particularly in Kazakhstan. These findings suggest that both biotic (feeding strategies) and abiotic (geographical and environmental conditions) factors significantly influence trace elements dynamics in freshwater ecosystems.
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