Structural Elements Research Articles

Images, Patterns and Audio: A Search for an Identifier of Genre Inclusive of Science Fiction

Genre is any style or form of communication in any mode (written, spoken, digital, artistic, etc.) with socially agreed-upon conventions developed over time. The need to understand what movie is for whom persists despite the blurring the genres in modern film. This paper analyses part of Susan Sontag’s essay “The Imagination of Disaster”, which deals with the structure of sci-fi movies, and Vivian Sobchack’s “Images of Wonder: The Look of Science Fiction”, which deals with the iconography of science fiction, through three movies – Ridley Scott’s Alien, Andrei Tarkovsky’s Solaris and Howard Hawks’ El Dorado. Beyond the structural elements revealed by Sontag, or the iconographic approach of Sobchack, audio is found to be a far reliable element that defines science fiction movies. Keywords: Science fiction, Alien, Solaris, western, genre, audio

Переходные участки бесстыкового пути, примыкающие к стрелочным переводам

Purpose: consider the problem of interaction between turnouts and adjacent sections of a seamless railway track. Methods: statistical data analysis. Results: an analysis of the results of domestic and foreign studies has been carried out, which show that in such areas there is a jump with subsequent extrusion of material of less rigidity and an increase in the slope of the elastic unevenness, i. e., an undesirable phenomenon occurs — a shock wave. The impact force depends on the mass of the rolling stock and the speed of movement, the geometric evenness of the rail track and the chosen approaches to designing the transition zone as a whole. Jump and mainly occurs due to compression and pushing out of structural layers and materials of lower rigidity in the under-rail area. The impact force depends on the mass of the rolling stock and the speed of movement, the geometric evenness of the rail track and the chosen approaches to designing the transition zone as a whole. Jump and mainly occurs due to compression and pushing out of structural layers and materials of lower rigidity in the under-rail area. Shock waves caused by uneven rigidity of sections have negative consequences (the appearance of deviations in level, subsidence, distortions, increased intensity of rail wear, the appearance of rail defects), which increase the cost of maintenance of the railway track. An analysis of the accumulation of residual deformations has been carried out, which shows the systematic formation of geometric irregularities in adjacent areas in the turnout area. Practical importance: from the point of view of the design of the transition zone, the expected advantage will be the differentiation of structural materials and elements and their influence on achieving the optimal gradation of railway track rigidity in the vertical and horizontal (transverse, longitudinal directions), selection of the optimal ratio of the mechanical properties of individual elements of the superstructure structure. It should also be noted that it is necessary to conduct an additional feasibility study of the feasibility of constructing transition sections in the area where the track and switches meet, taking into account the operational features of individual railway lines.

Improving the functional and technological properties of minced pork using a proteolytic enzyme

The use of pork obtained from intensively growing hybrid animals as the main raw material in meat processing, in particular sausage production, is accompanied by undesirable variability of consumer characteristics of sausage products and a decrease in the economic indicators of enterprise performance. The main technological disadvantages of processed pork are reduced water-binding capacity and significant loss of meat fluid, which in practice is usually compensated by the increased use of food additives and non-meat ingredients. The aim of the study was to assess the possibility of targeted improvement of the technological properties of minced pork using a proteolytic enzyme of animal origin. Model samples of minced meat were developed and cured for 24 hours at a temperature of 4 °C. The following control samples were used: minced meat with the addition of 2% edible salt and minced meat with the addition of 2% edible salt and 0.2% sodium bicarbonate. Moreover, 0.0001% chymotrypsin was added to the test samples containing similar curing ingredients. During electrophoretic study, in samples with the enzyme, an increase in low-molecular fractions (20 kDa, 15 kDa and lower) was observed, which indicated the manifestation of proteolytic activity of chymotrypsin in model systems. Enzymatic treatment led to an increase in pH and water-binding capacity. Cooking loss was reduced by 3 to 6 times, compared to Control 1. After cooking, histological studies of model systems showed that the test samples subjected to enzymatic treatment were characterized by a denser arrangement of structural elements, less pronounced cellular components of muscle tissue and the presence of glutin formed as a result of protein breakdown, filling the microcapillaries. Thus, the use of an enzyme preparation provides an opportunity for targeted improvement of the technological properties of pork obtained from intensively growing hybrid animals.

Productivity of Alternative Barley Genotypes under Variable Intraspecific Competition Resulting from Increasing Sowing Density

Sowing density and row spacing of barley affect the crop efficiency, resource use and final yield, with different genotypes likely to respond differently to this agrotechnical factor. The effect of sowing density on the barley yield, as shaped by structural yield elements such as the number of ears, number of grains per ears and thousand grain weight, depends on the interaction of this factor with the genotype and the growing conditions. Two spring barley genotypes with a black grain color (H. vulgare L. var. nigricans and H. vulgare L. var. rimpaui), differing in ear structure and affiliation to the original wild forms, were studied. Two independent, two-year field experiments were conducted in 2019–2020 and 2021–2022 at two locations with contrasting soil conditions. The effects of genotype and sowing density interactions on the yield, harvest index and structural elements of the yield were assessed. The arley yield was dependent on the interaction of genotype and sowing density but also varied by location. H. v. var. nigricans yielded better at higher densities, while H. v. var. rimpaui showed greater tillering potential at low densities. Environmental factors such as rainfall, temperature and soil composition affected the number of fertile ears, number of grains per ear and thousand grain weight.

Research on Coordinated Control of Vehicle Inertial Suspension Using the Dynamic Surface Control Theory

In the process of driving, the steering, braking, and driving conditions and different road conditions affect the vibration characteristics of the vehicle in the vertical, roll, and pitch directions. These factors greatly impact the riding comfort of the vehicle. Among them, the uneven distribution of vertical load between the left and right or the front and rear suspension is one of the important factors affecting the performance indicators of the vehicle’s roll angle acceleration and pitch angle acceleration. In order to improve the ride comfort of the vehicle in vertical, roll, and pitch motion, the inerter is introduced in this paper to form a new type of suspension structure with the “spring-damping” base element, inertial suspension. It breaks away from the traditional “spring-damping” base element of the inherent suspension structure. In this paper, the mechatronic inerter is taken as the actual controlled object, and the inertial suspension structure is considered as the controlled model based on the dynamic surface control theory and the pseudo-inverse matrix principle. Thus, the coordinated control of the inertial suspension can be achieved. Under random road input, compared with passive suspension, the ride comfort performance indicators of the vehicle with inertial suspension based on dynamic surface control are significantly improved. Finally, a Hardware-in-the-Loop (HiL) test of the controller based on dynamic surface control is carried out to verify that the performance of the vehicle inertial suspension using the dynamic surface control algorithm had improved in terms of vehicle ride comfort. The error between the experimental results and the simulation results is about 8%, which verifies the real-time performance and effectiveness of the dynamic surface controller in the real controller.

Constructing a Semantic System of Facade Elements for Religious Architecture from a Regional Perspective: A Case Study of Jingzhou

The application of semantics in facade elements mainly involves the association between architectural elements and their cultural, historical, or functional significance. By analyzing the shape, layout, and decoration of various elements (such as windows, doors, decorative patterns) in facades, semantics helps us understand the symbolic meanings and cultural implications behind these design choices. This study selects twenty-eight pavilions and buildings from five temples and Taoist sites in Jingzhou City as the research objects, exploring the composition and patterns of religious architectural facades in Jingzhou through the extraction of structural and decorative elements. The study establishes the “Semantic System of Façade Elements in Jingzhou Religious Architecture”, from which the distinctive characteristics of Jingzhou religious building façades are identified. The study finds that side halls predominantly feature hard gable roofs, while the main halls use double-eave hip-and-gable roofs, reflecting differences in architectural hierarchy. The sack with three arrows pattern is the most widely used in door and window decorations, demonstrating the aesthetic preferences of the Jingchu region. Both side halls and main halls commonly adopt high podiums, with the main hall podiums typically exceeding twenty steps in height, which is closely related to Jingzhou’s climatic conditions and architectural hierarchy. This study provides scientific evidence for the preservation, new design, and harmonious integration of traditional culture and architectural features in regional religious architecture.

THE SITUATION OF COMMITTING FRAUD UNDER EXTRAORDINARY LEGAL REGIMES

In the article, based on the analysis of forensic literature, the situation of committing criminal offenses is defined as a system of physical, material and social conditions of the environment chosen by the criminal or objectively surrounding him, in which the criminal offense occurs. It was noted that the quantitative and qualitative composition of the structural elements of the situation of committing criminal offenses depend on the types of criminal offenses. It is noted that fraudsters often adapt to typical factors in a similar environment, as a result of which a typical pattern of criminal activity appears. Regarding the situation and conditions of criminal offenses committed by fraud, it is noted that they are committed under the influence of certain economic, legal, social, political, psychological and other factors that create the basis for the unimpeded implementation of deception and contribute to the concealment of illegal actions. It was emphasized that the effectiveness of the entire investigation in the criminal proceedings as a whole often depends on how the investigator correctly analyzes the spatio-temporal characteristics and conditions in which the fraudsters operated.

Efficacy of a new customized navigation template for placement of transpedicular screws for unilateral access in children with congenital spinal deformity and thoracic malformation

BACKGROUND: Surgical treatment of congenital spinal deformities combined with thoracic development anomalies in children is an urgent and a complex problem in surgical practice. The surgical method of correcting congenital deformities caused by segmentation disorder of the lateral surfaces of vertebral bodies and unilateral rib synostosis is aimed at correcting the existing deformity using a transpedicular system. This technique is effective for treating this group of patients. However, it requires precise and accurate installation of the supporting elements of the metal structure owing to the high risk of damage to the neurovascular structures in the area of surgical intervention. A solution to this problem is the use of an individualized guide template with rib support for unilateral access in children with congenital deformities of the thorax and spine. AIM: To perform a comparative analysis of the results of using an individual rib-supported guide template for transpedicular screw placement and the “free hand” method in children during surgical correction of congenital spinal deformities accompanied by thoracic development anomalies. MATERIALS AND METHODS: The study included 14 patients who underwent surgical treatment for congenital deformity of the thoracic spine against the background of segmentation disorder of the lateral surfaces of the vertebral bodies in combination with thoracic anomalies. The patients were divided into two groups. Group 1 included six patients operated on using a new navigation template guide, and group 2 consisted of eight patients who underwent installation of support elements using the “free hand” method. The following parameters were compared: time spent at the stage of formation of bone canals for the support elements of the metal structure and accuracy and correctness of support element installation. Clinical data included demographic information and the size of the scoliotic arch, number of support elements, and presence of complications. Statistical analysis using Student’s t-test for unpaired samples or Mann–Whitney test was used to compare results. RESULTS: Data obtained in the course of the study confirmed the high efficiency and accuracy and showed that the use of the template reduces the time of bone canal formation, increases the accuracy, and provides greater correctness of the position of the supporting elements compared to the “free hand” method, which increases the efficiency and safety of surgical treatment. CONCLUSIONS: The developed template guide with support on the rib for the installation of the supporting elements of the metal structure in the surgical treatment of children with congenital deformity of the thoracic spine in combination with thoracic development anomaly showed better results compared to the use of the “free hand” method.

Identification of an osteopontin structural element for the restoration of a normal endometrial epidermal growth factor (EGF) profile determined by the EGF concentration on day 3 of estrous cycle and pregnancy outcome in repeat breeder dairy cows

The loss of a cyclic change with two peaks of increased endometrial epidermal growth factor (EGF) concentration on days 2−4 and 13−14 during the estrous cycle has been linked to low fertility in repeat breeder (RB) cows. We have shown that an intravaginal infusion of osteopontin (OPN) restored the EGF profile in RB cows. The present study aimed to determine a structural element of OPN to restore the normal EGF profile and fertility. Holstein RB cows were diagnosed the EGF profile by a single examination of the endometrial EGF concentration on day 3 of the estrous cycle. Those with an altered EGF profile were intravaginally infused with OPN and its fragments on the day of insemination (day 0); the concentration of endometrial EGF was measured on day 3, and pregnancy was diagnosed on days 30−35. In Study 1, recombinant OPN (rOPN) (16 nmol), thrombin-cleaved N- and C-terminal fragments of rOPN (N-rOPN and C-rOPN, respectively), and a combination of these fragments (Th-rOPN) were infused (n = 13−20). The restoration rate of the normal EGF profile of the N-rOPN group (25.0%) was a level in between the C-rOPN group (7.7%) and both the rOPN (55.6%) and Th-rOPN (64.3%) groups. In Study 2, PBS (n = 47), rOPN (9.5 nmol, n = 83), and peptides of integrin binding motifs, GRGDSVAYGLK (peptide 1; 32, 320, and 1,600 nmol), GRGDS (peptide 2; 320 and 1,600 nmol), and SVAYGLK (peptide 3; 320 and 1,600 nmol), were infused (n = 20−25). Restoration rates of the normal EGF profile of peptide 1 (320 and 1,600 nmol) and peptide 3 (1,600 nmol) groups (44.0−56.3%) were comparable with those of the rOPN group (63.9%) and higher than those of the PBS group (15.6%). Restoration rates of the other groups were similar to those of the PBS group. Additional cows received infusions to determine the effect on fertility. Conception rates of the peptide 1 (320 and 1,600 nmol; n = 50 each), peptide 3 (1,600 nmol; n = 55), and rOPN (n = 111) groups (41.8−50.0%) were comparable and higher than that of the PBS group (21.6%, n = 75). In Study 3, PBS (n = 24), peptide 1 (320 nmol; n = 78), and GRGESVAYGLK peptide (peptide 4; 320 and 1,600 nmol; n = 50 and 26, respectively) were infused. Restoration rates of the normal EGF profile of peptide 4 and PBS groups (16.0−19.2%) were comparable and lower than those of the peptide 1 group (44.9%). Thus, the SVAYGLK motif may be an OPN structural element to restore the normal EGF profile and fertility in RB cows, and the RGD motif may enhance its effect.

Optimising the embodied carbon of laterally loaded piles using a genetic algorithm and finite element simulation

With the need to cut embodied carbon from the construction industry, there has been a growing interest in optimising the design of different structural elements. This paper, for the first time, minimises the embodied carbon of laterally-loaded piles using a hybrid genetic algorithm. The research is split into four stages. Firstly, the optimisation algorithm is set and run to produce the optimal piles of lateral capacity 0.5 MN at a 25 mm lateral displacement limit, the embodied carbon of solid cylindrical and hollow cylindrical optimised pile designs is compared showing a significant reduction in the embodied carbon when the hollow geometry is used. Then an analysis is presented to test the effect of different displacement limits on the resulting embodied carbon, showing that the lateral displacement limit has a significant effect on the overall embodied carbon of the pile designs, highlighting a scope for reducing the embodied carbon if more pile displacement is permissible. A finite element model is built using ABAQUS simulation package to investigate the soil-pile interaction and compare the two optimal pile designs. Finally, the lateral load capacity of four different pile geometries is calculated to demonstrate the effect of pile geometry on its lateral load capacity, hollow cylindrical piles, showed to have the highest lateral load capacity compared to the other pile types. We conclude that the opportunity for significant carbon savings exists in laterally loaded piles through both optimisation and careful consideration of performance criteria.

You're More Resilient Than You Give Yourself Credit For: Building Resilience in Times of Change and Challenge

This article examines the concept of resilience and strategies for building resilience at the individual and organizational levels. It defines resilience as the ability to withstand challenges and adapt to change. The article explores how cultivating certain cognitive habits and support systems can enhance individual resilience. These include maintaining an adaptive mindset, developing strong social networks, finding purpose in one's work, and prioritizing self-care, especially during stressful periods. It also analyzes how structural elements and cultural dynamics within an organization can build its resilience. Attributes like flexible structures that enable adaptation, learning-oriented cultures, a clear sense of shared purpose, and resilient leadership styles are linked to an institution's capacity to manage challenges. The article provides practical recommendations for developing resilience through practices such as strengthening social connections, rewarding risk-taking and growth, openly acknowledging uncertainties, and role modeling resilient behaviors. It argues that proactively building resilience reserves empowers individuals and organizations to survive difficulties and emerge stronger.

Mechanical properties of pure elements from a comprehensive first-principles study to data-driven insights

Unraveling mechanical properties from fundamental is far from complete despite their vital role in determining applicability and longevity for a given material. Here, we perform a comprehensive study related to mechanical properties of 60 pure elements in bcc, fcc, hcp, and/or diamond structures by means of pure alias shear and pure tensile deformations via density functional theory (DFT) based calculations alongside a broad review of existing literature. The present data compilation enables a detailed correlation analysis of mechanical properties, focusing on DFT-based ideal shear and tensile strengths ( and ), stable and unstable stacking fault energies ( and ), surface energy (), and vacancy activation energy (); and experimental hardness (), ultimate tensile strength (), fracture toughness (), and elongation (). The present work examines models, identifies outliers, and provides insights into mechanical properties, for example, (i) is correlated by , by or , and by ; (ii) data outliers are identified for Cr (related to , , , and ), Be (, , , and ), Hf ( and ), Yb (all properties), and Pt ( vs. ); and (iii) , , , , , , and are highly correlated to elemental attributes, while , , and especially are less correlated due mainly to experimental uncertainty. In particular, the present data compilation provides a solid foundation to model properties such as and of multicomponent alloys and of unstable structures like bcc Ti, Zr, and Hf.

Footwear for Diabetics – Structural and Material Elements for the Prevention and Alleviation of Foot Lessions

Abstract Diabetic foot syndrome is a syndrome of specific conditions affecting the foot. It is a complication of diabetes. It occurs in 12–25% of patients with diabetes. Untreated, it leads to irreversible deformities and necrosis of the foot, often resulting in amputation. In this study the statistics and consequences of diabetic foot syndrome are described. Patients with diabetes need to take care of their lower limbs. Proper footwear can prevent foot wounds. Available solutions for the prevention and treatment of diabetic foot syndrome are presented herein: footwear, insoles and requirements for footwear materials. Appropriate equipment for a person with diabetes, especially one who has been diagnosed with diabetic foot syndrome or is at risk of such a condition, includes footwear and replaceable insoles. The parameters of footwear, insoles and footwear materials that are most optimal for patients with diabetes and diabetic foot syndrome were defined. The effect of a pulsed electromagnetic field and pulsed ultrasound on diabetic foot problems was evaluated.

Blue-button: A tool for institution-agnostic, EHR-integrated regional clinical trial matching.

65 Background: Research indicates that most people with cancer are willing to enroll in clinical trials when offered the chance, but only 7% actually participate in cancer treatment trials. Barriers to screening and enrollment include time and effort for providers to screen patients for trials, and lack of awareness of available regional trials. Stakeholders recommended creation of free, automated screening tools and processes integrated into provider workflows, directly interfacing with site electronic health records (EHRs), that would search for regional trial eligibility. The need for such tools was independently identified in a large national survey of oncology providers. Methods: The Blue-button tool was developed to automatically identify relevant, regional clinical trials. A collaboration between ACS CAN and MITRE, a not-for-profit organization working in the public interest, created the tool’s functionality. Clinical workflow and research protocol were developed in collaboration with University of Texas Southwestern Simmons Comprehensive Cancer Center. This SMART-on-FHIR application works within existing EHR systems by automatically extracting and sending limited, deidentified patient data elements to third-party trial matching services via the FHIR mCODE standard, a core set of non-proprietary, open-source structured data elements that establishes minimum standards for the structure and content of oncology data in health records. Potential trial matches within a specified radius of the practice are returned in a standard format using the FHIR Research Study resource. Results: Blue-button extracts limited patient data from EHR and provides it in an interface allowing users to update data before sending to trial matching services. Only non-identifiable information is sent to the external clinical trial matching services, which then return prescreen trial matches for manual review to confirm patient eligibility. Thus, with a few clicks in a medical record, the tool quickly identifies comprehensive trial availability within a specified distance of the patient’s location. When used by providers, Blue-button reduces the manual burden that often prevents consideration of clinical trials and identifies regional opportunities beyond the treating institution. Conclusions: Successful feasibility testing demonstrated the effectiveness of automated regional trial screening as a model. We have launched a clinical trial at two sites to assess the effectiveness of the Blue-button tool with patients in healthcare settings. We expect patient enrollment to continue through part or all of 2025. This trial of the Blue-button tool will provide additional information on provider accessibility and uptake and will demonstrate whether EHR-integrated regional trial screening leads to increased opportunities and enrollment in cancer clinical trials. Clinical trial information: NCT05885880 .

Quantifying the Vices and Virtues of Snakes and Ladders Through Time

Abstract The game of Gyan Chaupar or ‘Snakes and Ladders’ exists in many forms throughout history as a board game of varying size, structure, and game elements of snakes and ladders associated with various vices and virtues inscribed within the board. Three boards were analyzed via simulation in Python, including the 1998 Milton Bradley version, the 72-square Vaisnava board, and the 84-square Jaina board, with the goal of understanding the relationships between board design and associated behaviors and spiritual concepts. Game play on each board was simulated 100,000 times with variations that included individual removal of a snake or ladder, thereby quantifying the importance of that element towards achieving victory. Comparison of the weighted importance of each game element and associated vice and virtue permitted quantification of their importance for the game designer and their associated culture, with each game board component being assigned a contribution towards victory. Historical values inscribed within the class of Gyan Chaupar games were interpreted quantitatively via simulation allowing for comparison of different variations of the game and their associated cultures in different locations and time. The hypothesis that game element contribution towards victory could not be assessed by players a priori via element board length was supported by simulation data that identified many snakes and ladder game elements whose importance towards victory deviated significantly from proportionality with their game element length.

Genome-wide analysis and expression characteristics of Small Auxin-up RNAs genes in flax (Linum usitatissimum L.)

Plant auxin is crucial in regulating many aspects of plant growth and development. Small Auxin-up RNAs (SAURs) genes act as the main early auxin response genes and are involved in regulating plant growth and development. Flax is a well-known fiber and oil crop; thus, our study provided a systematic bioinformatics analysis of the SAUR family in flax, including basic parameters, gene structure, chromosomal location, phylogenetic analysis, and cis-acting element analysis. Based on genomic information, 86 LusSAUR genes were identified and separated into eight groups. The gene structure analysis revealed that only two LusSAUR genes have introns. The cis-acting elements related to hormones and stress were abundant in the promoter region of LusSAUR members. Based on the gene expression oligo microarray, LusSAUR genes had tissue-specific expression characteristics in eight different tissues. Quantitative real-time PCR analysis indicated that 10 selected LusSAUR genes could be quickly upregulated following treatment with IAA. The 10 genes displayed distinctive expression levels under hormone treatment (MeJA, ABA, SA) and abiotic stress (PEG, NaCl, CdCl2). This study provides a foundation for further investigations for functional analysis of LusSAURs in flax. Furthermore, it will provide useful insights for further research into the molecular mechanism of regulating hormones and environmental signals in flax growth and development.

Slow Conformational Exchange between Partially Folded and Near-Native States of Ubiquitin: Evidence for a Multistate Folding Model.

The mechanism by which small proteins fold, i.e., via intermediates or via a two-state mechanism, is a subject of intense investigation. Intermediate states in the folding pathways of these proteins are sparsely populated due to transient lifetimes under normal conditions rendering them transparent to a majority of the biophysical methods employed for structural, thermodynamic, and kinetic characterization, which attributes are essential for understanding the cooperative folding/unfolding of such proteins. Dynamic NMR spectroscopy has enabled the characterization of folding intermediates of ubiquitin that exist in equilibrium under conditions of low pH and denaturants. At low pH, an unlocked state defined as N' is in fast exchange with an invisible state, U″, as observed by CEST NMR. Addition of urea to ubiquitin at pH 2 creates two new states F' and U', which are in slow exchange (kF'→U' = 0.14 and kU'→F' = 0.28 s-1) as indicated by longitudinal ZZ-magnetization exchange spectroscopy. High-resolution solution NMR structures of F' show it to be in an "unlocked" conformation with measurable changes in rotational diffusion, translational diffusion, and rotational correlational times. U' is characterized by the presence of just the highly conserved N-terminal β1-β2 hairpin. The folding of ubiquitin is cooperative and is nucleated by the formation of an N-terminal β-hairpin followed by significant hydrophobic collapse of the protein core resulting in the formation of bulk of the secondary structural elements stabilized by extensive tertiary contacts. U' and F' may thus be described as early and late folding intermediates in the ubiquitin folding pathway.

Structural determinants of tendon multiscale mechanics and their sensitivity to mechanical stimulation during development in an embryonic chick model

There is an abrupt increase in the multiscale mechanical properties and load-bearing capabilities of tendon during development. While prior work has identified numerous changes that occur within the collagenous structure during this developmental period, the primary structural elements that give rise to this abrupt increase in mechanical functionality, and their mechanobiological sensitivity, remain unclear. To address this knowledge gap, we used a shear lag model along with ultrastructural imaging, biochemical/thermodynamic assays, and multiscale mechanical testing to investigate the dynamic structure-function relationships during late-stage embryonic chick development and to establish their sensitivity to mechanical stimulation. Mechanical testing and modeling suggested that the rapid increase in multiscale mechanics can be explained by increases in fibril length, intrafibrillar crosslinking, and fibril area fraction. To partially test this, we inhibited collagen crosslinking during development and observed a drastic reduction in multiscale mechanical behavior that was explained by a reduction in both fibril modulus and length. Using muscle paralysis to investigate mechanosensitivity, we observed a significantly impaired multiscale mechanical response despite minimal changes in fibril diameter and fibril area fraction. Additionally, the shear lag model found a trend toward lower fibril lengths with paralysis and experimental data found decreased crosslinking and fibril modulus values following flaccid paralysis. Together, these data suggest that both intrafibrillar crosslink formation and fibril elongation are critical to the formation of load-bearing capabilities in tenogenesis and are sensitive to mechanical loading. These findings provide critical insights into the biological and structural mechanisms that give rise to tensile load-bearing soft tissue. Statement of SignificanceDespite prior work investigating the structural and mechanical changes that occur during tendon development, there has not been a comprehensive analysis of how these simultaneous changes in structure and function are connected. In this study, we performed a comprehensive battery of mechanical and structural assessments of embryonic chick tendons and input these data into a shear lag model to estimate the individual importance of each structural change to the tendon mechanical properties. Additionally, we inhibited muscle activity in the embryos to evaluate the impact of mechanical stimulation on these evolving structure-function relationships during tendon development. These data provide insight into the primary structural elements that produce the tensile load-bearing capabilities of tendon, which will inform efforts to produce tissue engineered tendon replacements.

Comprehensive assessment of mercury contamination in bees, bee products and moss and lichen bags

Twenty-eight beekeepers around Slovakia were included in the research to evaluate the presence of mercury in honeybee bodies and selected bee-related products: bee pollen and honey. The samples were collected in May, June, and July (honeybee bodies only in May and June). During this period, moss and lichen bags for air quality assessment were exposed around the beehives and relative accumulation factor (RAF) was used for its evaluation. Mercury content in evaluated bioindicators was determined using AMA 254 analyser. Percentage of provisional tolerable intake (%PTWI) and target hazard quotient (THQ) were used to determine health risks related to bee pollen and honey consumption. Around the beehives the proportion of landscape structure elements was determined for each sampling locality, using a geographical informational system QGIS. The aim of the study was a comprehensive evaluation of the mercury content in the environment around beehives using several bioindicators and an assessment of the relationship between the presence of mercury pollution and the proportion of landscape structure elements in the vicinity of the beehives. The study also aimed to evaluate the transfer of contaminants between bee bodies and bee-related products and the health risks resulting from their consumption.

Structural adaptations for carboxypeptidase activity in putative S9 acylaminoacyl peptidase from Bacillus subtilis

Prolyl oligopeptidase, or S9 (MEROPS) family enzymes are crucial drug targets due to their association with various diseases, neurological disorders, cell growth, and survival. These implications render them an exceptionally fascinating field of research. Despite sharing similar structural features, they exhibit diverse enzyme activities, including endopeptidase, dipeptidyl peptidase, and acylaminoacyl peptidase. Additionally, a few members of the S9 family demonstrate carboxypeptidase activity. A recent study showed that the S9 peptidase of Bacillus subtilis (S9bs) possesses the conserved sequence feature necessary for carboxypeptidase activity despite being annotated as an acylaminoacyl peptidase in the UniProt database. However, the mechanism of action and identity of S9bs as carboxypeptidase remain unclear. Consequently, we focused our studies on thoroughly investigating S9bs for its carboxypeptidase activity. In the present study, we report biochemical and biophysical analyses of S9bs, confirming its identity as a carboxypeptidase. Further, structural analysis reveals the molecular basis of S9bs' carboxypeptidase activity, highlighting the crucial structural elements like the “cavity loop” and the “two-arginine” residues essential for this activity. Additionally, our studies confirmed that S9bs forms a stable tetrameric assembly and established its quaternary molecular arrangement, which reveals the presence of an oligomeric pore. Altogether, these structural features play a are crucial role in substrate selection for S9 carboxypeptidases. Overall, our findings reveal a distinct carboxypeptidase within the S9 family and significantly enhance our understanding of these enzymes. Moreover, this study sheds light on the mechanisms underlying carboxypeptidase activity, offering valuable insights that could contribute to therapeutic and drug design.