Tianshan is one of the world’s largest gold provinces; however, the relationship between gold mineralization and metasomatized subcontinental lithospheric mantle (SCLM) remains poorly understood. To improve our understanding, we present new bulk-rock geochemistry and platinum group element (PGE) concentrations of the SCLM-sourced Aksu Neoproterozoic diabase dykes in Chinese South Tianshan. These data, combined with in situ laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) analyses of hydrothermal pyrite grains in the diabase dykes, are used to discuss the SCLM source characteristics in the region and their potential links to formation of gold deposits. The diabase dykes exhibit high Th/Yb (0.47–0.62) and low Nb/U (13.4–16.3) ratios, indicating that magma evolution involves subduction-related fluid metasomatism and limited contamination of the continental crust. This is consistent with little variation in whole-rock Pd/Zr, Cu/Zr, and Ni/MgO ratios, suggesting that no sulfide segregation was caused by crustal contamination and magma mixing. In addition, the diabase dykes show low PGE and Au contents, with high Cu/Pd (>105) and low Cu/Zr (<0.5) ratios, indicating that magmas were derived from low-degree partial melting of the SCLM under S-saturated conditions. Such source characteristics indicate residual sulfides and chalcophile elements (e.g., PGEs, Au, and Cu) were concentrated at the SCLM reservoir in South Tianshan. Hydrothermal pyrite in the studied dykes has similar Au/Ag ratios and trace element distribution patterns to gold-bearing pyrite of lode gold deposits in Chinese South Tianshan, indicating that metasomatized SCLM may have contributed ore metals during the formation of these gold deposits. Adding to the available data, our study highlights that the SCLM may be a potential metal source reservoir, and it may have contributed to formation of the lode gold deposits in Chinese South Tianshan.