Structural Elements Research Articles

Problems with and Alternatives to Permeable (Open-Graded) Subbases for Use in Jointed Plain Concrete Pavement Structures

Due to their intuitive advantage to quickly remove water from a pavement structure, permeable (open-graded) subbases (subbases with a permeability greater than about 350 ft/day (107 m/day) in laboratory tests) have been a common design element in jointed plain concrete pavement structures since the 1990s. Despite this assumed advantage, permeable subbases are no longer recommended for inclusion in jointed plain concrete pavement structures for various reasons discussed in this paper. Freedraining subbases (subbases with more fines than permeable subbase and, thus, with a permeability less than 350 ft/day (107 m/day) in laboratory tests) that are daylighted are the reasonable alternative to permeable subbases with edge drainage systems.

3D polycatenated architected materials.

Architected materials derive their properties from the geometric arrangement of their internal structural elements. Their designs rely on continuous networks of members to control the global mechanical behavior of the bulk. In this study, we introduce a class of materials that consist of discrete concatenated rings or cage particles interlocked in three-dimensional networks, forming polycatenated architected materials (PAMs). We propose a general design framework that translates arbitrary crystalline networks into particle concatenations and geometries. In response to small external loads, PAMs behave like non-Newtonian fluids, showing both shear-thinning and shear-thickening responses, which can be controlled by their catenation topologies. At larger strains, PAMs behave like lattices and foams, with a nonlinear stress-strain relation. At microscale, we demonstrate that PAMs can change their shapes in response to applied electrostatic charges. The distinctive properties of PAMs pave the path for developing stimuli-responsive materials, energy-absorbing systems, and morphing architectures.

Influence of the Applied WC/C and CrN + WC/C Coatings on the Surface Protection of X2CrNi18-9 Cavitation Generators

The purpose of this paper is to investigate the impact of the applied WC/C and CrN + WC/C protective coatings applied using various PVD methods as protection for cavitation generators operating in an environment of intense cavitation wear. In order to carry out planned tasks, special devices generating a cavitation environment have been designed and manufactured. As part of this study, an analysis of the surface of cavitation generators, both before applying the coatings and with the applied protective PVD coatings, and also before and after operation in a cavitation environment, was carried out using the following research techniques: stereoscopic microscopy, scanning electron microscopy, transmission microscopy, XRD, and confocal microscopy. Despite the use of corrosion-resistant steels as a result of the cavitation environment, this causes surface material wear, especially in the area of the through holes. This is due to the fact that there are no protective coatings inside the through hole. Moreover, it was found that, for the tested steel with multilayer CrN + WC/C coatings, there were significantly fewer cavitation defects both on the surface of the material and on the edge of through holes, which indicates that the use of these multilayer coatings can significantly extend the service life of structural elements operating in such environmental conditions. Based on the conducted research tests, it was proven that the applying protective coatings significantly reduce the wear of the surfaces of the tested cavitation generators, thus allowing the use of cheaper steels, not resistant to corrosion, e.g., P265GH steel, which is five times cheaper than austenitic steel. The P265GH steel is used for structural elements in the heating, petrochemical, energy, food, and chemical industries, as well as for structural elements in the aviation, shipbuilding, and many other industries, and, thus, it is possible to reduce the costs associated with the operation of this construction solution in industrial conditions.

Enhanced RTP method for nonlinear statistical prediction of ship structural response

Abstract In this study, the Enhanced RAO-based Translation Process (ERTP) method, which is an enhanced version of the RAO-based Translation Process (RTP) method proposed in the previous study, is newly proposed. The RTP method approximates the Extreme Value Distribution (EVD) in arbitrary irregular waves from the nonlinear response in regular waves based on the idea of the transformation process, in which the nonlinear process is regarded as a transformation of a linear process. However, it was found that the RTP method is inaccurate when the response spectrum has multiple peaks, which is often the case for stresses in ship structures in waves. To solve this problem, the author developed the ERTP method, which defines a nonlinear transformation based on the L p-norm of the response spectrum. Direct Load and Structure Analysis considering the nonlinearity of wave loads was conducted and confirmed that the proposed method can reasonably estimate the EVD even for structural elements with a multi-peaked response spectrum with respect to the wave direction.

Soybean and avocado unsaponifiables: a review of their potential use in the treatment of osteoarthritis

Recent research has shown that Avocado-Soybean Unsaponifiables (ASU) greatly reduce the symptoms of osteoarthritis (OA). It’s yet unknown exactly how ASU works, however, it has been demonstrated to have analgesic and anti-inflammatory effects. These qualities can potentially lessen the need for non-steroidal anti-inflammatory medicines (NSAIDs) and their secondary effects. This review aims to examine the current literature on ASU, focusing on their efficacy, mechanism of action, and potential utility in treating OA for managing chronic pain associated with this condition. The literature review was conducted manually through Pubmed, Scopus and Web of Science (WOS) databases, covering studies from 2000 to 2022 with terms like “osteoarthritis,” “OA,” “animal models,” “ASU,” and “soy/avocado.” Two reviewers independently screened each article using inclusion and exclusion criteria and categorized the studies into in vitro, preclinical, and clinical groups. According to in vitro research, ASU affect the regulation of molecules related to OA, increasing structural elements like collagen and aggrecan and decreasing pro-inflammatory mediators. Although results vary, pre-clinical research in different animal models has demonstrated positive effects, such as ameliorating histopathological changes and reduced inflammation. Despite some discrepancies regarding structural changes in the joints, clinical trials typically demonstrate symptom relief and slow down the disease progression. While ASU demonstrates significant promise in alleviating OA symptoms and reducing reliance on NSAIDs, further research is essential to fully understand its mechanisms of action. More studies are needed to determine the precise pathways through which ASU exerts its effects and to establish the most effective dosages for its administration, either alone or in combination with other treatments.

Fluorescence Detection of DNA/RNA G-Quadruplexes (G4s) by Twice-as-Smart Ligands.

Fluorescence detection of DNA and RNA G-quadruplexes (G4s) is a very efficient strategy to assess not only the existence and prevalence of cellular G4s but also their relevance as targets for therapeutic interventions. Among the fluorophores used to this end, turn-on probes are the most interesting since their fluorescence is triggered only upon interaction with their G4 targets, which ensures a high sensitivity and selectivity of detection. We reported on a series of twice-as-smart G4 probes, which are both smart G4 ligands (whose structure is reorganized upon interaction with G4s) and smart fluorescent probes (whose fluorescence is turned on upon interaction with G4s). The fine mechanistic details behind the excellent properties of the best prototype N-TASQ remain to be deciphered: to investigate this, we report here on the synthesis and studies of two analogues, TzN-TASQ and AlkN-TASQ, and on a careful analysis of their G4-interacting properties, investigated both in vitro and in silico. Our results show that fine-tuning their constitutive structural elements allows for increasing the efficiency of both their 'off' (i. e., a conformation with a low fluorescence) and 'on' states (i. e., a conformation with a high fluorescence), which opens interesting ways for the design of more efficient fluorogenic G4 probes.

A Finite Element Method orthotropic multi-yield elastic-inelastic model of a wood-adhesive-steel composite

Composite elements made of wood reinforced by adhesively inter-layered steel lamina can be employed as structural elements in building engineering, thanks to their favorable ratios of strength and stiffness to mass and their ductility. The mechanical response of such composite, under loading, can be predicted by employing a Finite Element Method with a proper model of each material. In this work, the Finite Element Method model is formulated within the theories of Continuum Mechanics and irreversible thermodynamics of deformation, finite strains hypothesis, and kinematics of large displacements. For the wood material constituent an orthotropic elastic–plastic-damage constitutive law is adopted, to address the effects of irreversible strains, formulated by a multi-surface yield, both for plasticity and damage, where each yield surface operates disjointedly each other, at the level of stress–strain component. The validity of the proposed model and its computational technique is revealed by analyzing the stress–strain path until the failure of a composite element. Thus, the numerical results are compared with the experimental data obtained in a tension test of that composite element. The proposed model adequately represents the mechanical behavior of the composite.

Challenges and Lessons Learned From Pseudo‐Dynamic Hybrid Simulations on Ductile Steel Braced Frame Systems

ABSTRACTPerformance‐based seismic design, which emerged more than two decades ago, requires accurate numerical models to capture the response of structural elements that undergo inelastic deformations under random loading histories. High‐fidelity benchmark test results under real natural hazards are therefore required to assist researchers and practitioners with this effort. Substructuring pseudo‐dynamic hybrid simulation (PsDHS) is an efficient, yet effective testing method for evaluating the system‐level response of structures under extreme loading scenarios, and for forming a database of high‐fidelity benchmark test results. In PsDHS, the response of the critical structural components is captured in a laboratory through physical testing and is integrated with the numerical response of the remainder of the structure in a numerical model, by establishing a communication framework between the two. The former is referred to as a physical substructure and the latter is often referred to as the integration module. Despite its efficiency and effectiveness, large‐scale hybrid simulation introduces researchers to a range of non‐trivial challenges, especially in laboratories that are new to the methodology. This paper presents challenges and lessons learned from 21 large‐scale pseudo‐dynamic hybrid simulations on different ductile steel braced frame systems including a buckling‐restrained braced frame (BRBF), a special concentrically braced frame (SCBF), a yielding brace system (YBS) equipped with cast steel yielding connectors (YCs), and eccentrically braced frames (EBFs) designed with cast steel replaceable modular yielding links (CMLs). Details for each hybrid simulation including the experimental setups, reference buildings, earthquake records, etc. along with selected results are presented. Challenges that were faced in each hybrid simulation related to hardware, the control system, integration schemes, etc., and attempted solutions are discussed. The findings from each set of hybrid simulations on each braced frame system are summarized. The experimental results are organized as a dataset in an online data repository, which is available for download. The organization of the dataset is presented to facilitate access to the experimental results. In the end, concluding remarks and visions for future research are presented.

Traduction du discours humaniste dans Les Déboires de Dieudonnée de Francis Beng Nyamnjoh

This study stemmed from the observation that English-speaking Cameroonian literature makes use of humanist discourse among many others to denounce the failings of leaders and the misery of peoples in a bilingual and multicultural context. Translating, therefore, texts imbued with humanist discourse could be a challenge for the literary translator, despite being an intercultural mediator. With regard to this skill, does the translator’s strategy succeeded in preserving the essence and richness of the original text destined from the start for a French readership? In this light, the analysis below examined the translation and meaning preservation of the structuring elements of the said discourse in Francis Nyamnjoh's Les Déboires de Dieudonné. Thus, based on Berman’s Translation Critism which convened Touré's Descriptive Translation Studies (DTS) and focusing on few extracts taken from the aforementioned corpus, our analyses aim first to identify the structuring elements of Nyamnjoh's humanist discourse, then to describe the strategy and procedures employed by the translator, and finally to justify in the post-colonial literature framework, the scope of the author's discourse in French. The results obtained after this study shows that the translator used 86% of foreignization, against 14% of naturalization. These results lead us to conclude that literal translation is par excellence, the indispensable procedure in the translation of literary works, in other words it remains the guarantor of a scrupulous morphosyntactic analysis and a correct semantic analysis of the lexemes in the text.

Concrete strengthening with external prestressed metal straps: A critical review

AbstractExternal confinement can be used to strengthen, repair, and rehabilitate concrete buildings by providing stress laterally. Among various confining techniques, prestressed metal strap (PTMS) stands out as a cost‐effective and eco‐friendly active confining material. Numerous experimental and numerical analyses have been carried out to understand the capabilities of PTMS confinements. To systematically report the current research state of PTMS‐confined concrete, this review collected and analyzed the data obtained from previous research. This paper outlines the effects of PTMS on the strength and ductility of different types of structure elements, as well as the parameters that influence its effectiveness. The durability and seismic resistance of PTMS‐confined concrete are reviewed. This paper also examines the confinement models commonly used or proposed for PTMS‐confined specimens, hybrid techniques applied along with PTMS, and finite element analyses of PTMS‐confined structures. The findings have reached a consensus that PTMS is effective in improving or restoring the strength, ductility, and seismic performance of both intact and damaged concrete structures. More experimental and analytical works, such as durability testing, have to be conducted, and finally, problems of insufficient flexural stiffness improvement and limited effectiveness on prismatic specimens should be addressed to ensure the reliability of PTMS confinements. Additionally, service life estimation and life cycle cost analysis are recommended to assess the practicality of PTMS in real‐world construction.

Seismic evaluation and retrofit of reinforced concrete structures

The destructive earthquakes in the world clearly reveal the importance of the seismic behavior of the structures. The current study proposes and numerically investigates a systematic technique for the seismic assessment and retrofit of reinforced-concrete buildings according to both material strain limit approach and Quadrants assessment method. In this study numerical analysis were made for two three-dimensional building models, namely a single-storey 30 years old existing RC building in Koyna-Warna area (Zone-IV) in India, and an earthquake-resistant designed 4-story RC building. The structural analyses were performed for two 3-D models of the selected buildings are analysed. Nonlinear static adaptive pushover analysis method was used in Seismostruct software program for two different buildings. While one of the buildings is an existing building, the other is chosen as a new building. Structural characteristics and deficiencies of the investigated single-storey existing reinforced-concrete structure necessitated seismic evaluation. The deficiencies include pitched roofs, re-entrant corners, and structural distress such as structural element cracking and cover concrete spalling, among others. The single-story RC building is retrofitted with RC jacketing and fiber-reinforced polymer sheet wrapping, while the four-storey RC building does not need to be retrofitted based on the Quadrants assessment method. Also, the significant seismic design parameters are evaluated before and after the retrofit. The results show that the material strain limit technique when combined with the Quadrants assessment method makes a more efficient technique for seismic assessment and retrofit purpose. Also, the ultimate capacity and over strength factor of the retrofitted single-storey RC existing building are found 2.04 times and 1.82 times increased in X and Y direction as compared to the building without retrofit, because of the RC jacketing and FRP sheet wrapping.

Examining the Ability of Digital Visual Art Engagement to Cultivate Empathy and Social Connection

We aimed to impact social connectedness and perspective taking using visual art-based psychoeducational materials and skill-building exercises. Participants ( N = 381) were assigned to one of three conditions. Within the perspective taking condition, people took different viewpoints when viewing art. Within the social connection condition, people considered how art can help in reflecting on their relationships. Within the art education control, people focused on structural elements when viewing art. People completed 4 virtual gallery visits and measures of empathy, social connection, and loneliness at baseline, following each visit, and one-week post intervention. Participants reported their level of immersion and reflectiveness following each visit. Results suggest the conditions did not show changes one week post intervention; however, there were interesting findings post-visit. First, reflection was highest in the perspective taking condition, and the social connection condition showed increases in reflection across time. Second, the social connection condition showed reductions in loneliness.

Методика обучения знакообразованию в контексте овладения навыками устной речи на примере школьного обучения

The article deals with the methodology of teaching sign formation in the context of mastering oral speech skills using the example of school education. A decisive role in the proper organization of sign formation training is played by a preliminary careful selection of structures to be mastered and their location in a certain scientifically based order. In the course of school education, only the absolutely necessary minimum of structures should be selected for activation, without which it is impossible to do in the practice of oral communication. The new structure should be introduced mainly based on the material of already mastered ready-made signs of the language. The structure being introduced may include lower-order structures that have already been mastered; for example, the new sentence structure will include previously worked-out types of syntagmas. The introduction, activation and re-consolidation of structures should be strictly planned. The efficiency of work on mastering the technique of physical reproduction of signs of the sound language increases many times with the rational use of sound recording and cinema tools. A word can appear in two functions: either as a component of a ready-made or typical senior sign, memorized as a whole, or as a wildcard element of a productive structure. Hence, it is clear that for the learner, the word should not exist as a unit of vocabulary or dictionary and not as a caption to a drawing. By memorizing ready-made and typical senior signs of the language, rather than isolated words, we benefit in at least three ways: first, senior signs, as we already know, are those "blocks" that make up language communication; Secondly, it is only together with the older signs of the language and through them that productive grammatical structures are assimilated and consolidated; thirdly, it is only through the use of a word in a syntagma or sentence that its exact meaning is revealed.

Digital Image Correlation and Reliability-Based Methods for the Design of Structural Beams Made from Recycled Concrete Using Aggregates from Precast Rejects

The use of recycled aggregates in the manufacture of concrete is presented as a solution to reduce the consumption of resources and waste in the construction sector and contribute to a lower environmental impact. This work aims to explore the possibility of producing structural beams from recycled concrete using aggregates from precast concrete rejects and to improve their design using advanced characterisation techniques. To this end, the experimental data coming from mechanical test and the use of the digital image correlation approach are combined with a robust reliability-based method. The full-field data provided by the digital image correlation approach allow to determine the probabilistic density functions of the mechanical data. From these data, a predictive analysis of the maximum strength and deflection of flexural beams is carried out based on robust design techniques. This approach uses analytical theoretical models and a Monte Carlo-based simulation strategy that allows the prediction of the behaviour of the beams. This methodology was validated by manufacturing six beams with the previously analysed recycled concrete, HA-30, and testing them in the laboratory. All the beams showed behaviour within the predicted range: around 49.7 kN maximum load and just over 9.3 mm maximum deflection. These results demonstrate the robustness of the approach as well as the feasibility of using precast rejects for the manufacture of structural elements.

Mechanistic determinants and dynamics of cA6 synthesis in type III CRISPR-Cas effector complexes.

Type III clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems (type III CRISPR-Cas systems) use guide RNAs to recognize RNA transcripts of foreign genetic elements, which triggers the generation of cyclic oligoadenylate (cOA) second messengers by the Cas10 subunit of the type III effector complex. In turn, cOAs bind and activate ancillary effector proteins to reinforce the host immune response. Type III systems utilize distinct cOAs, including cyclic tri- (cA3), tetra- (cA4) and hexa-adenylates (cA6). However, the molecular mechanisms dictating cOA product identity are poorly understood. Here we used cryoelectron microscopy to visualize the mechanism of cA6 biosynthesis by the Csm effector complex from Enterococcus italicus (EiCsm). We show that EiCsm synthesizes oligoadenylate nucleotides in 3'-5' direction using a set of conserved binding sites in the Cas10 Palm domains to determine the size of the nascent oligoadenylate chain. Our data also reveal that conformational dynamics induced by target RNA binding results in allosteric activation of Cas10 to trigger oligoadenylate synthesis. Mutations of a key structural element in Cas10 perturb cOA synthesis to favor cA3 and cA4 formation. Together, these results provide comprehensive insights into the dynamics of cOA synthesis in type III CRISPR-Cas systems and reveal key determinants of second messenger product selectivity, thereby illuminating potential avenues for their engineering.

Interplay between Cα Methylation and Cα Stereochemistry in the Folding Energetics of a Helix-Rich Miniprotein.

The α-helix is an abundant and functionally important element of protein secondary structure, which has motivated intensive efforts toward chemical strategies to stabilize helical folds. One such method is the incorporation of non-canonical backbone composition through an additional methyl substituent at the Cα atom. Examples of monomers include the achiral 2-aminoisobutyric acid (Aib) with geminal dimethyl substitution and chiral analogues with one methyl and one non-methyl substituent. While Aib and chiral Cα-Me residues are both established helix promoting moieties, their comparative ability in this regard has not been quantitatively investigated. Addressing this gap would help to inform the use of these building blocks in the construction of peptide and protein mimetics as well as provide fundamental insights into consequences of backbone methylation on folding. Here, we report a quantitative comparison of the impacts of Aib and chiral αMe residues on the high-resolution folded structure and folding thermodynamics of a small helical protein. These results reveal a synergistic stabilizing effect arising from the presence of Cα methylation in conjunction with a Cα stereocenter.

Boosting the energy storage performance of BCZT-based capacitors by constructing a Schottky contact.

Multilayer thin films composed of dielectric Ba0.7Ca0.3Zr0.2Ti0.8O3 (BCZT) and oxygen-deficient BCZT (BCZT-OD) were fabricated on (001)-oriented NSTO substrates using the pulsed laser deposition (PLD) technique. Unlike conventional approaches to energy storage capacitors, which primarily focus on compositional or structural modifications, this study explored the influence of the layer sequence and periodicity. The interface between the NSTO substrate and the BCZT-OD layer forms a Schottky barrier, resulting in electric field redistribution across the sublayers of the BCZT/BCZT-OD//(1P) thin film. This redistribution delays the breakdown of the BCZT layer, significantly enhancing the film's electric breakdown strength. Furthermore, mathematical analysis reveals that the electric field redistribution amplifies dipole polarization, with BCZT-OD-initiated multilayers exhibiting superior polarization compared to those with equivalent periodicity but different starting layers. Consequently, the BCZT/BCZT-OD//(1P) multilayer achieves an exceptional recoverable energy density (Wrec) of 150.22 J cm-3 and an energy efficiency (η) of 83.07%, surpassing typical performance benchmarks for BCZT-based thin films. These findings are corroborated by comprehensive structural characterization studies, performance evaluations, and finite element simulations, which further validate the role of the Schottky barrier in enhancing voltage endurance. Analogous to "Tian Ji's Strategy for Horse Racing", this work achieved high Wrec by sacrificing the ferroelectricity of the negative side of the P-E loop, introducing an innovative paradigm for designing and developing next-generation electronic devices.

Noncanonical RGS14 structural determinants control hormone-sensitive NPT2A-mediated phosphate transport.

The sodium phosphate cotransporter-2A (NPT2A) mediates basal and parathyroid hormone (PTH)- and fibroblast growth factor-23 (FGF23)-regulated phosphate transport in proximal tubule cells of the kidney. Both basal and hormone-sensitive transport require sodium hydrogen exchanger regulatory factor-1 (NHERF1), a scaffold protein with tandem PDZ domains, PDZ1 and PDZ2. NPT2A binds to PDZ1. RGS14 persistently represses hormone action by binding to PDZ2. The RGS14 canonical RGS domain, Ras/Rap-binding domains, and G protein regulatory motif cannot explain its regulatory effects on hormone-sensitive phosphate transport because these actions are mediated not only by the PTH receptor, a G protein-coupled receptor (GPCR), but also by the fibroblast growth factor receptor-1, a receptor tyrosine kinase that is not governed by G protein activity. Here, we identify the structural elements of RGS14 that mutually control the action of PTH and FGF23. RGS14 truncation constructs lacking upstream sequence and the RGS domain were fully functional. Removing the linker sequence between the RGS and RBD1 domains abolished RGS14 action. Examination of the alpha-helical linker region suggested candidate serine residues that might facilitate regulatory activities. RGS14 Ser266 and Ser269 are phosphorylated in response to PTH and FGF23, and replacement of these residues by Ala eliminated the actions of RGS14 on hormone-sensitive phosphate transport. PTH stimulated the phosphorylation of a peptide construct harboring the sites of purported phosphorylation and full-length RGS14. Mutating Ser266Ala and Ser269Ala abolished phosphorylation. The results establish that RGS14 regulation of phosphate transport requires targeted phosphorylation within the linker and an intact PDZ ligand.

Recent Developments in Azetidinone-Azole Conjugates: Emerging Antimicrobial Potentials.

The emergence of multidrug-resistant microbial strains poses a significant challenge to global public health. In response, researchers have been exploring innovative antimicrobial agents with enhanced efficacy and novel mechanisms of action. One promising approach involves the synthesis of hybrid molecules combining azetidinone and azole moieties, capitalizing on the respective antimicrobial properties of both structural elements. Natural and synthetic azetidinone derivatives hold a prominent position among medicinally significant compounds due to their varied and potent antibiotic activities. Interest persists in discovering new synthetic methods and refining existing ones, as well as applying these methods to create novel, biologically active azetidinone derivatives. Additionally, azoles are highly regarded in pharmaceuticals for their broad efficacy, tolerability, and oral availability. By merging these two pharmacophores, researchers aim to create compounds with synergistic or additive antimicrobial effects, potentially overcoming existing resistance mechanisms. Various synthetic strategies, including click chemistry and multicomponent reactions, have been employed to prepare these hybrid molecules efficiently. The antimicrobial potential of azetidinone-azole conjugates has been extensively evaluated against a spectrum of pathogens, including bacteria, fungi, and protozoa. These studies have demonstrated promising results, with several compounds exhibiting potent activity against both Gram-positive and Gramnegative bacteria, as well as clinically relevant fungal strains. Furthermore, SAR studies have provided valuable insights into the key structural features governing the antimicrobial properties of these conjugates, facilitating further optimization and rational design. In conclusion, the development of azetidinone-azole hybrids represents a promising avenue in the quest for novel antimicrobial agents. This study presents a comprehensive overview of recent advancements in synthesis and antimicrobial evaluation of azetidinone-azole conjugates.

Study of the SPL gene family and miR156-SPL module in Populus tomentosa: Potential roles in juvenile-to-adult phase transition and reproductive phase.

Populus tomentosa, a deciduous tree species distinguished by its significant economic and ecological value, enjoys a wide-ranging natural distribution. However, its long juvenile period severely restricts the advancement of breeding work. The SPL gene family, a distinctive class of transcription factors exclusive to the plant kingdom, is critical in various processes of plant growth and development. The miR156-SPL molecular module stands as an indispensable regulatory mechanism in the transition from the vegetative juvenile phase to the adult phase in plants. Consequently, this research endeavored a methodical and exhaustive exploration of the SPL gene family within the P.tomentosa species, synergistically integrating the miR156 family into the analysis. A total of 56 PtSPL genes were identified and subjected to a comprehensive analysis of their gene structure, conserved motifs, collinearity relationships, chromosomal localization, and promoter cis-acting elements. Further analysis of gene expression profiles confirmed the pivotal role of PtSPLs in the reproductive phase and tissue development of P. tomentosa. In addition, 11 members of miR156 in P. tomentosa were identified and their sequences analyzed, elucidating the miR156-SPL regulatory network. The target relationship between miR156k and PtSPLs was further validated by detecting the expression levels of PtSPLs in transgenic poplars overexpressing 35S::MIR156k. This comprehensive study lays a robust theoretical foundation for the continued exploration and application of the SPL genes in P. tomentosa, opening avenues for future research and potential advancements in plant biology and breeding strategies.