The object of this study was concrete samples of the cathedral and wall frescoes. The study solved the problem related to the destruction of concrete and wall frescoes under the long-term influence of biochemical and climatic factors. Samples of concrete for research and wall murals were obtained from a historic listed building. Using microbiological studies and scanning electron microscopy, damage to wall murals and concrete by microscopic fungi was established: Aspergillus fumigatus, Penicillium brevicompactum, Aspergillus niger, Cladosporium sphaerospermum. The study of concrete samples by the TPD-MS method showed the presence of an increased level of moisture and carbon compounds by 20 % in the test samples, compared to control. The sulfur content in all concrete samples was not significant. Determination of the mineral composition of concrete by X-ray diffraction showed the presence of Al2O3, 36–44 %, which indicates a significant clay content. The presence of NiTi, 53 %, and СoMg7O8, 46 %, in the concrete sample indicates the probable migration of the chemical elements of the paint pigments used to decorate the cathedral. The concrete control sample contained a significant amount of SiO2, up to 51 %, which is the main component of sand. A feature of the work is the determination of the corrosion effect on concrete under prolonged exposure to climatic and biological factors. The present study is distinguished by the use of non-destructive methods: microbiological studies, scanning electron microscopy, TPD-MS and X-ray diffraction to determine the destruction of concrete and wall frescoes of the building, which is a cultural heritage. The results of the study could be applied to the development and planning of restoration works for the restoration of buildings that have historical value