For the deflection analyses of thin-walled Timoshenko laminated composite beams with the mono- symmetric I-, channel-, and L-shaped sections, the stiffness matrices are derived based on the solutions of the simultaneous ordinary differential equations. A general thin-walled composite beam theory considering shear deformation effect is developed by introducing Vlasov’s assumptions. The shear stiffnesses of thin-walled composite beams are explicitly derived from the energy equivalence. The equilibrium equations and force-deformation relations are derived from energy principles. By introducing 14 displacement parameters, a generalized eigenvalue problem that has complex eigenvalues and multiple zero eigenvalues is formulated. Polynomial expressions are assumed as trial solutions for displacement parameters and eigenmodes containing undetermined parameters equal to the number of zero eigenvalues are determined by invoking the identity condition to the equilibrium equations. Then the displacement functions are constructed by combining eigenvectors and polynomial solutions corresponding to nonzero and zero eigenvalues, respectively. Finally, the stiffness matrices are evaluated by applying the member force-displacement relations to the displacement functions. In addition, the finite beam element formulation based on the classical Lagrangian interpolation polynomial is presented. In order to verify the validity and the accuracy of this study, the numerical solutions are presented and compared with the finite element results using the isoparametric beam elements and the detailed three-dimensional analysis results using the shell elements of ABAQUS. Particularly the effects of shear deformations on the deflection of thin-walled composite beams with the mono-symmetric I-, channel-, and L-shaped sections with various lamination schemes are investigated.