This study describes the opposite charge electrospun method for preparation of polyacrilonitrile / DMF nanofibrous tubular structure . Constant a processing parameters such as high polymer concentration and high rotational speed mandrel collector with varied the applied voltage and flow rate in wide range to display the effect of each one in determining the diameter , alignment, porosity, mean surface area and mean pores size of nanofibers. Tubular structures were produced from highly aligned continuous and uniform nanofibers through the opposite charge electrospinning methods and effect of each regulating parameter on tubular structure properties were evaluated and characterized using scanning electron microscope (SEM) and Fast Fourier Transform (FFT) and thresholding method. Increasing flow rate of polymer solution considerably increased the diameter of the nanofibers , ranges of the average diameters were larger , the nanofibers diameter distribution were gradually border. Nanofibers diameter on the other hand, decreased as the applied voltage increased, ranges of the average diameters were smaller and the nanofibers diameter distribution were gradually narrower . while increased the porosity % , heights intensity(Y%) and mean surface area (nm2) and decreased the mean pores size (nm) with increased both the applied voltage and flow rate of polymer solution