Electrophoretic Patterns Research Articles

Improving Interpretation Consistency of Serum Capillary Electrophoresis by Development of Quantitative Graphic Indexes.

Capillary zone electrophoresis-immunosubtraction (CZE-IS) is an essential laboratory test in diagnosing plasma cell neoplasms. However, the current interpretation of the test results is subjective. To evaluate CZE-IS in a more precise manner, this study proposed five key indexes, namely sharpness index, light chain index, immunoglobulin G index, immunoglobulin A index, and immunoglobulin M index. The reference intervals of these indexes were established using CZE-IS curve data from a clinical laboratory of a referral medical center. A total of 1000 cases with normal electrophoretic patterns were sampled for reference intervals establishment, and an additional 20 cases were included for validation. The following reference intervals in the γ zone were established: 1-6 (sharpness index), 1.06-2.71 (light chain index), 37-454 (immunoglobulin G index), (-9)-41 (immunoglobulin A index), and (-16)-46 (immunoglobulin M index). For the β2 zone, the reference intervals were 3-17 (sharpness index), 0.44-1.90 (light chain index), (-7)-61 (immunoglobulin G index), 2-117 (immunoglobulin A index), and (-12)-35 (immunoglobulin M index). The diagnostic performance of reference intervals of the proposed indexes in validation ranged from 95% to 100%. CZE-IS indexes provide the objective quantification of key characteristics of CZE-IS curves and improve the precision of CZE-IS interpretation.

Prevalence of monoclonal proteins in patients with isolated hypogammaglobulinemia on serum protein electrophoresis

Isolated hypogammaglobulinemia (IH) is an electrophoretic pattern that can be encountered on serum protein electrophoresis (SPEP) and is defined as a decreased but morphologically normal γ-globulin fraction with normal α- and β-globulin fractions. SPEP is mainly used to detect monoclonal proteins which are usually observed as additional peaks in the electropherogram. However, they may also be more discretely present in a significant proportion of patients presenting with IH. Therefore, we aimed to evaluate i) via both retrospective and prospective analysis to what extent paraproteins as identified by immunofixation are present in patients demonstrating IH on SPEP and ii) whether other parameters may predict their presence in IH-patients. For this purpose, we first reviewed historic SPEP- and immunofixation results in our tertiary hospital and determined paraprotein prevalence in this retrospective cohort. This analysis showed immunofixation was requested in only 519/3938 (13.2%) historic IH-results with 52/519 (10%) patients demonstrating paraproteins. Next, various laboratory parameters were compared between paraprotein-positive and -negative patients and subjected to logistic regression models but regrettably, no parameter could be retained as promising predictor of paraproteins. Lastly, to confirm paraprotein prevalence seen in the historical query, we conducted a six-month prospective analysis during which immunofixation was requested more frequently in IH-cases during routine diagnostics and which showed paraproteins to be present in 20/83 (24.1%) of IH-patients. Hence, as up to 24% of patients with IH may harbour paraproteins, one should consider performing follow-up analyses (e.g. immunofixation, urine electrophoresis and/or free light chain analysis) for all IH-cases identified via SPEP.

Toxicological effects and potential reproductive risk of microplastic-induced molecular changes in protamine-like proteins and their DNA binding

Today, plastic pollution is a widespread problem in all ecosystems and has a particularly severe impact on marine ecosystems and external fertilisers such as the mussel Mytilus galloprovincialis. The present study aims to assess the toxicological reproductive health effects in this organism following exposure to two concentrations of polystyrene microplastics (PS-MPs) (0.5 and 1 μg/L), representative of conditions in the Mediterranean Sea. After exposure, the electrophoretic pattern of protamine-like (PL) proteins, the major basic protein component of Mytilus galloprovincialis sperm chromatin, was analysed. Compared to the unexposed condition, differences were observed by SDS-PAGE and an increased ability of PL to bind and protect DNA from oxidative damage was then measured, particularly for PL from mussels exposed to 1 μg/L PS-MPs. At this dose of PS-MPs, a reduced release of all PLs from the sperm nuclei was also observed, whereas the digestion by micrococcal nuclease did not show any significant differences between the exposed and the unexposed conditions. Finally, the possibility of poly(ADP)-ribosylation of the PLs was investigated. PL-II showed an increase in poly(ADP)-ribosylation after PS-MPs exposure, which may account for the difference in the ability of the PLs to bind DNA. In conclusion, while all the results might suggest a molecular mechanism of gametic plasticity occurring upon exposure of mussels to PS-MPs 1 μg/L, they also indicate that this dose of exposure could be extremely detrimental to the reproductive health of Mytilus galloprovincialis because it could prevent the release of basic nuclear proteins from the sperm DNA at fertilisation.

B-057 Evaluating the Use of Unconcentrated Urine for Protein Electrophoresis and Immunofixation: An Alternative Approach for Efficient Analysis

Abstract Background Protein electrophoresis is a widely employed technique for separating and analyzing proteins based on their charge and size. Traditionally, concentrated urine samples have been utilized to enhance protein detection sensitivity. However, this study explores the feasibility of using unconcentrated urine as an alternative sample type for protein electrophoresis (UEL) and immunofixation (UIF) to streamline the analysis process and reduce costs and turnaround time in clinical laboratories. It offers a more efficient and resource-friendly alternative. Methods Both UEL and UIF were performed on routinely ordered 80 urine specimens (random or 24 Hr.) for patient management and performed by both concentrated (X40) by Helena and unconcentrated using Sebia (HYDRAGEL 9IF gels using HYDRAGEL BJ dynamic mask procedure. The electrophoretic patterns obtained from unconcentrated urine were compared with those from concentrated urine. Various electrophoretic parameters, including resolution, sensitivity, and reproducibility, were evaluated to determine the reliability of unconcentrated urine in protein electrophoresis. In addition, retrospective analysis was performed before and after implementation on all urine specimens ordered to analyze if there were any differences in the monoclonal band distributions in the population. Results All the protein concentrations were correlated well with 95-100% agreement between the methods (Figure 1) clear separation of protein fractions. Serial dilution studies of monoclonal band analyses showed that these bands can be detected at 1.25 mg/dL using an unconcentrated method. Retrospective analysis of patient data before and after the implementation of the new method shows no differences in the identification of monoclonal proteins (Helena [69 of 872 total patients tested (8.0%)] vs. Sebia [90/800 (11.0%)]. Conclusions These results indicate that unconcentrated urine can be successfully utilized for UEL and UIF with comparable outcomes to traditional concentrated samples. Utilizing unconcentrated urine simplifies the sample preparation process and presents a promising alternative.

Antioxidant analysis of protein from fresh and dry leaf of Orthosiphon aristatus (Blume) Miq.

Orthosiphon aristatus (Blume) Miq. is a folklore plant consumed as brewed tea for various health benefits. The white variety of the plants leaf and stem are predominantly sold in the market in dried form, blooming as a natural herbal product. To date, no proteomics and antioxidant studies are available on the fresh and dried leaf protein extract of O. aristatus since most studies take an interest in the crude extracts of plants. Thereupon, this study focuses on the One-dimensional (1D) electrophoretic pattern of the fresh and dry leaf determined via sodium dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Additionally, antioxidant proteins are currently being focused on for their link to controlling disease through their potential to destroy free radicals that are present in excess. Hence, an antioxidant assay was conducted for the fresh and dry leaf protein extract using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical assay and ferric reducing antioxidant power (FRAP) assay. The electrophoretic pattern obtained resulted in approximately 16 and 10 bands for the fresh and dry leaves respectively. As for the antioxidant activity, the dry leaf had higher radical scavenging activity and higher reducing power compared to the fresh leaf. The findings obtained provides insight in to the protein content and antioxidant activity found in the protein extract of fresh and dry leaves of O. aristatus. Hence, further detailed study of the protein extracts could further unveil their therapeutic potential.

Chitosan-collagen biopolymer biofilm derived from cephalopod gladius; Evaluation of osteogenesis, angiogenesis and wound healing for tissue engineering application

Chitosan (Ch) and acid-soluble collagen (ASC) from Doryteuthis singhalensis gladius were isolated to test their osteogenic, angiogenic, and wound healing capabilities in male Wistar rats. The results of the study showed that the ASC yield was 18.58 %, the total protein content was 86.43 % ± 0.18 %, and the amino acid composition was as follows: glycine, 15.68 %; proline, 13.84 %. A, B, I, II, and III show FT-IR amide regional bands at 3392, 2959, 1652, 1471, and 1237 cm−1 respectively. The electrophoretic pattern of ASC validated its molecular weights of 105 kDa and 96 kDa. The 1H NMR spectra showed pure singles at 1.99 ppm, and the UV–Vis spectrum showed a particular absorbance between 238 and 220 nm. The DSC showed two endothermic peaks: one with an To value of 119.72 °C and TP of 126.28 °C, and the other with 147.42 °C and 148.47 °C. Initially, we fabricated Ch and ASC biofilms at an 8.5:1.5 ratio for tissue engineering applications. A cellular-level study demonstrated good biocompatibility and enhanced osteoblastic differentiation of collagen chitosan films (CChF). Additionally, the biofilm exhibited increased angiogenic potency, as observed in the chick embryo chorioallantoic membrane (CAM) assay. The experimental animal model demonstrated that in wound healing, the CChF treated rats (95.75 ± 2.28 %) had a greater decrease in the diameter of the wound than the control rats (22.25 ± 2.45 %), followed by the CF (collagen film) treated rats (63.25 ± 2.08 %) and ChF (chitosan film) (52.67 ± 1.58 %). Rats treated with CChF had 48.82 ± 1.25 mg/g of hydroxyproline in NFGT and 75.25 ± 1.56 mg/g of overall protein. The higher hydroxyproline levels in the CChF-treated groups corroborated these histopathological findings. These results imply that by promoting the development of scars, inflammation, and proliferation, CChF accelerates the healing process.

DELINEATING GENETIC DIVERSITY IN SOYBEAN (GLYCINE MAX L.) GENOTYPES: INSIGHTS FROM A-PAGE ANALYSIS OF GLOBULIN RESERVE PROTEINS

This study explores the genetic diversity of soybean (Glycine max) genotypes using polyacrylamide gel electrophoresis (A-PAGE) to analyze globulin reserve proteins. The research utilized 51 soybean genotypes from various origins, including Canada, Turkey, Uzbekistan and others. The A-PAGE method was employed to extract and analyze the globulin storage proteins in the grains of soybean samples. The proteins were categorized into ω, γ, β and α zones, revealing distinct electrophoretic patterns among the genotypes. A total of 22 spectra and 107 patterns were identified, demonstrating polymorphism based on the frequency of occurrence. The genetic diversity index, calculated using Nei’s formula, indicated varying levels of genetic diversity across the different zones.

GENETIC DISI'ANCE MEASURES AMONG FIVE INDIGENOUS CATTLE BREEDS IN NIGERIA

Genetic variation is the basis of effective improvement in farm animals. Population differentiation is used for objective choice of parental genotypes that constitutes new hybrids in crossbreeding. In Nigeria, population characteristics of selected indigenous cattle breeds have not been fully documented. Therefore, blood protein electrophoretic patterns of selected indigenous cattle breeds in Nigeria were assessed. Blood samples (5mL) were taken underneath the tail by venipuncture from 200 cattle (40/breed) randomly selected from the five indigenous cattle breeds. The samples were subjected to cellulose acetate electrophoresis to determine the genetic variants of haemoglobin (Hb), carbonic anhydrase (CA) and transferrin (TO. Data were analysed using Euclidean genetic distance and cluster analysis. Two main clusters from the dendrogram were observed for each of Hb, CA and TE Euclidean genetic distance at the Hb locus between WF and SG, WF and RB, WF and BK, WF and Muturu were 15.3, 15.7, 23.9 and 35.1 respectively, while the corresponding values at CA locus were 3.9, 3.3, 29.1 and 36.2, similarly, the corresponding values at Tf locus were 22.8, 25.8, 94.3 and 102.7. Genetic distance estimates were found to be higher between non-related breeds than between breeds which had common ancestors.

Changes in RNase activities and in expression of two RNase genes in powdery mildew infected barley, wheat and Brachypodium distachyon leaves

AbstractChanges in RNase activities were investigated in extracts from barley near isogenic lines without or with various powdery mildew resistance genes and were compared to changes in wheat and Brachypodium distachyon leaves after powdery mildew infections. In barley, the compatible interaction with powdery mildew induced the highest increase in RNase activity as measured spectrophotometrically. The incompatible interaction that accompanied with hypersensitive reaction in Mla leaves gave less increase, whereas incompatible interactions in Mlg and mlo barley leaves without visible symptoms gave the least increase of RNase activity. In wheat, the largest RNase activity was found in leaves infected with the compatible wheat powdery mildew or wheat stem and leaf rusts. RNase activity in B. distachyon was higher than that in healthy wheat and especially barley leaves. The electrophoretic RNase enzyme activity patterns were different in barley, wheat and B. distachyon plants, but showed similar activities as determined spectrophotometrically. Barley genes encoding endonuclease 2 and ribonuclease 3-like protein X3 showed the highest expression in the compatible barley - barley powdery mildew interaction as measured by RT-qPCR. This correlated with RNase activities in leaf extracts suggesting that RNases in barley and wheat may act as susceptibility factors of powdery mildew and rust diseases.

Gluten-free crackers using chickpea and pumpkin seed press cake blend: Effects of gastrointestinal digestion

The use of a blend of chickpea and pumpkin seed press cake flours in the production of gluten-free crackers represents a promising strategy to address nutritional gaps and meet the growing demand for healthier gluten-free options. To test this assumption, an in vitro digestion method was employed to assess changes in total phenolic content, antioxidant activity, protein composition, electrophoretic patterns, and mineral content of gluten-free crackers formulated using these raw materials before and after digestion. The crackers were produced substituting 20% and 35% of chickpea flour by using two types of pumpkin seed press cake flour, one from virgin oil pressing (VFC) and the other from cold-pressed oil pressing (CFC). This approach aimed to assess how the treatment history of the pumpkin seed press cake flour affected the tested parameters. All samples displayed a 3- to 4-fold increase in total phenolic content after in vitro digestion. A noticeable enhancement in antioxidant activity is observed, where CFC samples showed higher values compared to VFC samples. The control sample and 35CFC sample present similar protein digestibility values (%), 76.97 and 77.05 respectively, while other crackers showed significantly lower digestibility. Incorporation of pumpkin seed press cake flours leads to a substantial increase in mineral content, notably zinc (up to 2-fold), iron (up to 40%), and magnesium (up to 2.5 fold). Overall, the study provides valuable insights into the utilization of pumpkin seed press cake as a potential source of bioactive compounds, emphasizing its significance beyond its traditional role as a by-product.

Caracterización de Meloidogyne spp. y densidad poblacional de nematodos fitoparásitos asociados a cultivos de aliáceas en la región Arequipa, Perú

Alliaceae crops includes several species of agricultural importance for the Arequipa region, among them the onion is the most produced in the country, however, these crops are affected by phytoparasitic nematodes, including Meloidogyne spp. which causes significant losses in the yield. The aim of the present study was to characterise the root-knot nematode (Meloidogyne spp) and determine the population density of phytoparasitic nematodes in alliaceae crops in the main producing localities of the Arequipa region. Samples of roots and soil were collected in onion, chinese onion, leek and chives crops in the provinces of Arequipa, Castilla, Caylloma and Islay. The identification of nematode genus was found by morphological characteristics and the characterization of Meloidogyne were performed by perineal patterns and electrophoretic pattern of esterase (Est). In the 38 soil samples, different genus of plant-parasitic nematodes was found: Aphelenchus, Dorylaimus, Helicotylenchus, Hemicycliophora, Meloidogyne, Mesocriconema, Mononchus, OGMA, Pratylenchus, Rotylenchus, Tylenchus, Xiphinema and free-living nematodes, in root samples, eight populations of Meloidogyne spp. were found. distributed among three species: (1) M. incognita phenotype Est I1, Rm: 1.0 with 33.33% of frequency and Est I2, Rm: 1.03, 1.07 (50%); (2) M. javanica Est J3, Rm: 1.03, 1.24, 1.37 (16.67%) and an atypical species named Meloidogyne sp. Rm: 0.85 (33.33%). The identification of genus and species phytoparasitic nematodes in alliaceae will allow the adoption of more efficient integrated management measures, because have a distinct parasitism behavior.

Comparative Evaluation of Protein Values and Electrophoretic Patterns of Buffalo Milk and Cow Milk Proteins by the SDS-PAGE Method

Comparative Evaluation of Protein Values and Electrophoretic Patterns of Buffalo Milk and Cow Milk Proteins by the SDS-PAGE Method

Artemia salina Biological Responses to Zinc Oxide Nanoparticles

The Zinc oxide nanoparticles could be entered and separated in various water bodies through the runoff. The aim of this investigation is to evaluate the influences of different concentrations of zinc oxide nanoparticles on Artemia salina (used as live feed in aquaculture) at developmental, biochemical and molecular levels. The homogeneity among the estimated. Artemia salina was estimated using Inter simple sequence repeats and some isozyme polymorphism. The results showed that samples were highly homogeneous. The hatching percentages decreased as the concentration of Zinc Oxide nanoparticles increased, compared to the control group. The maximum hatching percentage (75.88%) was detected at the control followed by ZT1(65.52%), ZT2 (55.87%) and ZT3(55.22%). The percentage of each developmental stage of A. salina under the experimental conditions, including egg, breakage egg, umbrella stage, Instar 1, Instar 2 and Instar 3, was calculated. Also, the impacts of ZnO NPs on A. salina isozyme variations were evaluated. The results showed some different A. salina tissue responses among the various ZnO NPs concentrations. The total number of bands was 15, 12, 10 and 5 for a-Esterase, b-Esterase, Superoxide dismutase and Lactate dehydrogenase isozymes respectively. The electrophoretic pattern of the A. salina Esterases shows remarkable biochemical markers. Understanding such influences could be helpful for the industry to make informed decisions on whether to use such Zinc oxide nanoparticles or find safer alternatives, ultimately affecting production costs and profitability. The industry should pay attention on developing safer nanoparticles and optimizing their concentrations to reduce long-term costs associated with species depletion and environmental damage.

Reversal of Valproate-Induced Major Salivary Gland Changes By Moringa Oleifera Extract in Rats.

This study aimed to explore the potential protective impacts of Moringa oleifera extract on major alteration in salivary glands of rats exposed to sodium valproate (VA). Groups were defined as control, control+moringa extract, sodium valproate, and sodium valproate+moringa extract. Antioxidant and oxidant status, activities of digestive and metabolic enzymes were examined. VA treatment led to various biochemical changes in the salivary glands, including decreased levels of antioxidants like glutathione, glutathione-S-transferase, and superoxide dismutase (except for sublingual superoxide dismutase). Conversely, a decrease in alpha-amylase, alkaline and acid phosphatase, lactate dehydrogenase, protease, and maltase activities were observed. The study also demonstrated that VA induces oxidative stress, increases lipid peroxidation, sialic acid, and nitric oxide levels in the salivary glands. Total oxidant capacity was raised in all glands except in the sublingual gland. The electrophoretic patterns of proteins were similar. Moringa oleifera extract exhibited protective properties, reversing these VA-induced biochemical changes due to its antioxidant and therapeutic attributes. This research suggests that moringa extract might serve as an alternative treatment approach for individuals using VA and experiencing salivary gland issues, although further research is necessary to confirm these findings in human subjects.

A Comparative Study of Electrophoretic Patterns of Proteins in Different Tissues of Fresh Water Cat Fish Heteropneustes fossilis (Bloch)

A Comparative Study of Electrophoretic Patterns of Proteins in Different Tissues of Fresh Water Cat Fish Heteropneustes fossilis (Bloch)

Association of Serum Proteins Electrophoretic Pattern and Serum Hormones in Women with Spontaneous Pregnancy Loss.

Pregnancy is the state of carrying a developing embryo or fetus within a female body. Once pregnancy is established, a range of endocrinological events appear in its maintenance, finally helping in the successful pregnancy. The complications which are usually observed in pregnancy are gestational diabetes, preeclampsia, preterm labor, and spontaneous pregnancy loss or miscarriage, while 10%-15% of clinically recognized pregnancies terminate into spontaneous miscarriage. Thus, many attempts have been made by different researchers for the diagnosis of high-risk pregnancy on altered protein pattern using placental villous tissue or follicular fluid, but these are difficult to obtain and results of different studies are not constant. This study was designed to identify the association (if any) among serum protein(s) electrophoretic pattern and different serum hormones in normal pregnant women (controls) and gestational age-matched women with spontaneous pregnancy loss (cases). This study was carried out for 1½ year from October 2018 to March 2020 and included 120 participants (60 normal pregnant women and 60 women with spontaneous pregnancy loss) between 20 and 45 years of age with no mean age difference. The electrophoresis of serum was carried out using slab gel electrophoretic unit and serum thyroid-stimulating hormone (TSH), total tri-iodothyronine (TT3), total thyroxine (TT4), prolactin, and beta human chorionic gonadotropin (β-hCG) levels were analyzed using TSOSH AIA analyzer at Adesh University, Bathinda. Significant variations in the expression of proteins with molecular weight around ~150 kDa, ~50 kDa, and ~25 kDa were observed in normal pregnant women and women with spontaneous pregnancy loss. However, the protein band of ~50 kDa was found to be highly expressed in the serum of 1st and 2nd trimester women experiencing spontaneous pregnancy loss. Therefore, selected protein band of ~50 kDa was further processed by ECI-mass spectrophotometry QUAD time of flight and 365 different proteins were found, out of these; 34 proteins were found to be unidentified protein products (Verified using NCBI data base). Further, TT3, total proteins, β-hCG, and prolactin level were found to be low, whereas, TSH was found to be high in women experiencing spontaneous pregnancy loss. However, difference in the level of β-hCG in the 1st trimester and TT4 among normal pregnant women and women with spontaneous pregnancy loss was observed to be statistically insignificant. This study indicated that the evaluation of serum protein variations along with hormonal profile may provide valuable information about high-risk pregnancy. Moreover, the differential expression of proteins in women with spontaneous pregnancy loss can be further explored to develop potential biomarker for the early identification of high-risk pregnancy and appropriate preventive measure.

Creating a novel genetic diversity of Trichoderma afroharzianum by γ-radiation for xylanase-cellulase production

Creating novel sources of a microbial strain using induced mutation can increase enzyme production for industrial use. According to this, we have developed a mutant strain of Trichoderma afroharzianum by Co60 gamma irradiation. Trichoderma mutants were isolated from an optimum dose of 250 Gy. The qualitative and quantitative screening were used for evaluating their enzyme production and the DNA barcoding method was used to identify the best Trichoderma mutant isolates. The highest cellulase (exo-glucanase, endoglucanase, β-glucosidase, and total cellulase) and xylanase activities were observed in superior mutant isolates of Trichoderma afroharzianum NAS107-M44 and Trichoderma afroharzianum NAS107-M82, which is approximately 1.6–2.5 times higher than its parent strain, respectively. The electrophoretic pattern of proteins showed that the exo-glucanase I, endo-glucanase III, and the xylanase I enzymes hydrolyzed the corn bran, synergistically. Overall, gamma irradiation-induced mutation could be an expedient technique to access such superior mutants for the bioconversion of corn bran wastes.

Effects of freeze-thaw cycles on the quality of Hot-dry noodles: From the moisture, starch, and protein characteristics

Freeze-thaw cycles (FTC) could cause damage to food during storage. The effects of different FTC on Hot-dry noodles (HDN) in terms of quality, moisture, starch, and protein characteristics were studied. This study showed that FTC decreased the texture properties and water absorption of HDN. Meanwhile, cooking loss was significantly increased after FTC. The water content of HDN was decreased and water migration was increased during FTC. In addition, results showed that FTC destroyed the order structure and increased the crystallinity of starch in HDN. Under FTC, the disulfide bond of HDN was broken, the free sulfhydryl group was increased, and the electrophoretic patterns confirmed the protein depolymerization. The microstructure also showed that the gluten network became incomplete and starch was exposed outside the substrate. This study expounded the mechanism of HDN quality deterioration during FTC, which laid a foundation for the development and improvement of frozen and freeze-thaw noodles.

Biochemical Characterization of the Seed Quality of a Collection of White Lupin Landraces from Southern Italy

Lupin species provide essential nutrients and bioactive compounds. Within pulses, they have one of the highest contents of proteins and fibers and are among the poorest in carbohydrates. The Mediterranean region is an important cradle area of the origin and domestication of cultivated white lupin (Lupinus albus L.). In this work, we present the characterization of 19 white lupin landraces collected from several sites in southern Italy, characterized by different pedoclimatic conditions. The protein contents and electrophoretic patterns, total polyphenols, phytic acid, lipids and phosphorous content, and reducing and anti-tryptic activities have been determined for each landrace. The relationships of the compositional characteristics, the area of origin of landraces and between compositional characteristics and thermo-pluviometric trends that occurred in the genotype comparison field during the two-year period between 2019 and 2020 are compared and discussed. From a nutritional point of view, some of the analyzed landraces differ from the commercial reference. The panel of molecular analyses performed can help in building an identity card for the grain to rapidly identify those varieties with the desired characteristics.

Esterase Inhibition Study in Different Tissues (Intestine, Muscle and Brain) of Fresh Water Cat Fish Heteropneustesfossilis through Polyacrylamide Gel Electrophoresis

Esterase polymorphism was studied in different tissues of stinging catfish (Heteropneustesfossilis) viz; intestine, muscle and brain. Intestine showed ER esterases(Esterases resistant to inhibitors) and CE esterases (Carboxylesterases), muscle showed ChE (Cholinesterases) and CHspesterases (Enzymes which were inhibited by Paraoxon, Eserine, and pCMB) and brain showed ChE(Cholinesterases) and Esdp(Esterases inhibited by Eserine alone) esterases. The present study was carried out to find out tissue specific esterase banding patterns in different tissues of H.fossilis. Electrophoretic banding patterns of esterase of different tissues showed species specific variation which could be successfully used for identification of fish species. Electrophoretic pattern of esterases of different tissues show species specific variation, it could be successfully used for the identification of fish species