We present an electronic tongue system composed of an analog front-end (AFE) with embedded calibration and temperature compensation for interfacing with an array of Ion-Sensitive FETs (ISFETs) or Enzyme – Extended Gate FETs (EEGFET). The AFE consists of a floating bridge type constant voltage constant current (CVCC) structure, and transmission gates as sensor enable. It also includes a temperature readout circuit and an instrumentation amplifier for temperature compensation. The calibration algorithm is synthesized into a Field Programmable Gate Array (FPGA) and implemented onto an Application Specific Integrated Circuit (ASIC). The system shall be used to measure the pH and the concentration of calcium ions in a solution alongside a conductometric sensor system. The ISFETs used are from ITE-Poland and shall be utilized to measure pH; meanwhile, to measure the Ca2+ concentration, EEGFETs with calcium ionophore on polyvinyl chloride (PVC) on SnO2/ITO/Glass thin film have been fabricated. The performance of the system using pH buffer solutions of pH 4–10, and CaCl2 buffer concentrations of 0.001–1M has been verified. The sensors and the AFE readout exhibited a Nernstian response (~50mV/pH) with a very stable output having a relative standard deviation of just ~−0.4%, and a Ca2+ sensitivity of 25.02mV/pCa, with a drift of −6.82mV/M-s. An on-chip version of the AFE using TSMC 0.35µm CMOS 2P4M Technology is also presented as well as the ASIC implemented on TSMC 0.18µm CMOS 1P6M technology.