Background: The World Health Organization defines alcohol-related diseases as the third major cause of death and disability worldwide accounting for approximately 2.5 million deaths per year. Immune dysregulation has possible role for pathogenesis of alcohol liver injury. We aimed to study lymphocyte subsets in alcoholic liver disease (ALD). Materials and Methods: A prospective cross-sectional study was done over 2 years in a tertiary care hospital in Western Maharashtra. The cases were classified into four groups: nonalcoholics (n = 18), fatty liver (n = 13), alcoholic hepatitis (n = 11), and alcoholic cirrhosis (n = 51). ALD was confirmed by abdominal ultrasonography and liver function tests. Blood sample collected in ethylenediaminetetraacetic acid was run on electronic cell counter for total leukocyte count and on flow cytometer for CD45, CD3+, CD4+, and CD8+ counts. Results: Hemoglobin and platelet count showed a statistically significant reduction (P Conclusion: In this study, we found a decrease in the concentration of lymphocytes and its subpopulation (CD3+, CD4+, and CD8+) while as CD4+/CD8+ ratio was comparatively increased in ALD patients in comparison to nonalcoholics. These findings signify an immune dysregulation in ALD.