Electron Microscopic Findings Research Articles

Is it really descemetocele? Morphology of extremely thin membrane that remained after severe corneal melting: a case report.

The aim of this study was to report transmission electron microscopic findings of a case with whole corneal descemetocele following infective corneal ulcer for the first time in literature. A 72-year-old male patient presented with infective corneal ulcer. After resolution of the infection, corneoscleral transplantation was performed. The excised very thin corneal membrane was processed for transmission electron microscopic examination. Transmission electron microscopic examination of the specimen revealed many layered structures that consisted of two different types of cells. The first type consisted of lighter staining polygonal cells, while the second consisted of elongated cells with relatively dense staining. All cells were connected with a large number of gap or adherens junctions with intercalation of the cell membranes of adjacent cells. A haphazard distribution of cytoplasmic microfilaments were also observed in all of the cell types. There was no evidence of the presence of endothelial cells throughout the specimen. There was also no evidence of Descemet membrane presence except for a small part adjacent to iris tissue that contained some melanosomes. Although we clinically diagnosed descemetocele, Descemet membrane was not present at the electron microscopic level, and thus, the expression "descemetocele" is inappropriate.

Synthesis, pharmaceutical properties, and in silico study of ZnO@TiO2 nanocomposite

The use of environmentally friendly production of nanoparticles has recently interest of scientists. The primary goal of this study is to create core–shell nanoparticles zinc oxide @ dioxide titanium (ZnO @ TiO2) for some biomedical application by a two-stage Nd: YAG pulsed laser ablation, the first stage involves laser ablation of a Zinc target to produce ZnO nanoparticles (the core) in colloidal form. Subsequently, in the second stage, TiO2 (the shell) is ablated within the colloidal ZnO Nanoparticles. The transmission electron microscope (TEM) findings approve the synthesis of ZnO @ TiO2, displays an average particle size of 13 nm for ZnO particles, 20 nm of TiO2NPs, and 34 nm of the ZnO@TiO2 NPs. The anti-bacterial efficiency was assessed against the bacterial strains' Bacillus cereus, Proteus mirabilis. Our results discovered inhibition zone of 19 mm and12.50 mm for Bacillus cereus, Proteus mirabilis, correspondingly, when by ZnONPs. Whereas inhibition zone of 20.50 mm and 21.50 mm for Bacillus cereus, Proteus mirabilis, correspondingly, when by TiO2NPs. While with ZnO@TiO2NPs, result was better to 27.50 mm for Bacillus cereus and 24 mm for Proteus mirabilis. The antioxidant efficiency was assessed by DPPH, The results show have strong antioxidant activity for ZnO @ TiO2 core–shell than ZnONPs alone, TiO2NPs alone. Cytotoxicity assay established a significant reduction in cancer cell population pole 72hr. Cytotoxicity of ZnO@TiO2NPs on breast cancer cells was 90.23 %. Computational innovation of TiO2, ZnO and ZnO@TiO2 nanoparticles against breast cancer line gave the highest correlation value and RMSD between ZnO and 6NLV receptors from other nanocomposites.

Toxicological assessment of a novel spiro‐derivative, 4‐amino‐1‐azaspiro[4.5]dec‐3‐en‐2‐one for the greater wax moth, Galleria mellonella

AbstractThe greater wax moth, Galleria mellonella L., poses a substantial threat to apiculture, adversely impacting wax quality and potentially acting as a disease vector. There is an urgent need to develop innovative and effective control strategies to address the challenges posed by this pest and ensure the sustainability of the apiculture industry. Spiro‐derivative compounds represent a novel class of environmentally safe compounds with high insecticidal properties. For example, they have demonstrated high efficacy against sap‐sucking insects such as aphids and whiteflies. However, their effect on chewing insects has not yet been well explored. In this study, we hypothesized that 4‐amino‐1‐azaspiro[4.5]dec‐3‐en‐2‐one (4A1AD), a spiro‐derivative compound, would exhibit efficacy against G. mellonella larvae. To test this hypothesis, we conducted dipping and feeding bioassays, incorporating varying concentrations of 4A1AD on fourth instar G. mellonella larvae, and recorded mortality, biochemical parameters including total soluble protein content and enzyme activities, and anatomical abnormalities resulting from treatment. Results revealed a significant increase in larval mortality with increased concentrations and treatment durations across both bioassays. Additionally, a significant decrease in protein content and an increase in phenol‐oxidase activity were observed with alterations in α and β‐esterase activities. Scanning electron microscope findings revealed abnormalities in larval cuticle, spiracular openings and legs. These findings demonstrated the efficacy of 4A1AD, indicating a potential alternative to conventional insecticides in promoting sustainable apiculture practices. Moreover, they provide valuable contributions to the development of novel strategies for effective pest management in apicultural settings.

A case of fulminant cryofibrinogenemia with rapid renal dysfunction and toe necrosis

BackgroundThere are few reports of kidney disease caused by cryofibrinogen (CF). There are still many unknowns regarding its diagnosis, treatment, and prognosis.Case presentationA woman in her 70s experienced gross hematuria without any triggers; no urinary abnormalities had been previously detected. At the same time, the urine protein level was 5 g; therefore, a renal biopsy was performed. Light microscopy revealed a membranoproliferative glomerulonephritis-like pattern. And the electron microscopic findings were extremely characteristic and specific. Development of ischemic lesions was observed in the lower legs. A skin biopsy performed at the sites of toe necrosis showed fibrinoid necrotizing vasculitis and thrombi in the blood vessels. Eventually, the patient was diagnosed with cryofibrinogenemia (CF-emia) by mass spectrometry. The effect of steroids was limited. Subsequently, the patient’s renal function rapidly deteriorated, and toe necrosis progressed. The patient died after initiation of hemodialysis.ConclusionAlthough CF-emia is an unknown disease and has been infrequently reported, no reported cases exhibited rapid worsening of toe necrosis and renal function during the same period. Therefore, this case can be said to be the first case of fulminant cryofibrinogenemia. Due to the development of nephropathy, which is likely to be a factor for poor prognosis, establishment of therapeutic strategies is urgently required.

Sulphuric precipitates in novel titanium-based, sulphur-bearing bulk metallic glass – a BMG composite?

ABSTRACT New titanium-based metallic glasses containing sulphur as a key element have recently been developed and are the subject of ongoing research related to the effects of sulphur introduction into glass-forming alloy systems. Here we report on the formation of sulphuric precipitates during solidification of a Ti40Zr35Cu17S8 alloy and on the occurrence of secondary intermetallic compound crystallization starting in sulphur-depleted zones around the precipitates. The alloy is a promising metallic glass for use as a structural and biomedical material as it shows a high yield strength and good corrosion resistance paired with a low density. The role of sulphur for the glass formation in the Ti-Zr-Cu-S system is discussed and specimens quenched into different structural states are investigated by means of high-energy synchrotron diffraction and electron microscopy. The electron microscopic findings reveal that sulphuric precipitates precede the crystallization of an intermetallic (Ti, Zr)2Cu phase, which is the primary phase when sulphur is not present in the system and thus lead to a higher glass-forming ability. The sulphuric precipitates are found in specimens of various casting thickness and differ in their volume percentages depending on the size of the cast sample, while the secondary phase (Ti, Zr)2Cu crystallizes heterogeneously in the interfaces between the sulphuric precipitates and the local matrix.

Antibacterial activity and biosynthetic potential of Streptomyces sp. PBR19, isolated from forest rhizosphere soil of Assam.

An Actinomycetia isolate, designated as PBR19, was derived from the rhizosphere soil of Pobitora Wildlife Sanctuary (PWS), Assam, India. The isolate, identified as Streptomyces sp., shares a sequence similarity of 93.96% with its nearest type strain, Streptomyces atrovirens. This finding indicates the potential classification of PBR19 as a new taxon within the Actinomycetota phylum. PBR19 displayed notable antibacterial action against some ESKAPE pathogens. The ethyl acetate extract of PBR19 (EtAc-PBR19) showed the lowest minimum inhibitory concentration (MIC) of ≥ 0.195µg/mL against Acinetobacter baumannii ATCC BAA-1705. A lower MIC indicates higher potency against the tested pathogen. Scanning electron microscope (SEM) findings revealed significant changes in the cytoplasmic membrane structure of the pathogen. This suggests that the antibacterial activity may be linked to the disruption of the microbial membrane. The predominant chemical compound detected in the EtAc-PBR19 was identified as phenol, 3,5-bis(1,1-dimethylethyl), comprising 48.59% of the area percentage. Additionally, PBR19 was found to contain the type II polyketide synthases (PKS type II) gene associated with antibiotic synthesis. The predicted gene product of PKSII was identified as the macrolide antibiotic Megalomicin A. The taxonomic distinctiveness, potent antibacterial effects, and the presence of a gene associated with antibiotic synthesis suggest that PBR19 could be a valuable candidate for further exploration in drug development and synthetic biology. The study contributes to the broader understanding of microbial diversity and the potential for discovering bioactive compounds in less-explored environments.

Examination of upper respiratory tracts of Siirt-coloured mohair goats by scanning electron microscopy before and after plastination.

Present study was conducted to determine the changes in the surface structure of the upper respiratory tract of Siirt-coloured mohair goats by the silicone plastination method. Accordingly, the heads of 10 Siirt-coloured mohair goats procured from slaughterhouses were divided into two halves. Half of each head was plastinated. After macro-comparisons were made, the deformations of silicone plastination on the surface were examined by comparing the scanning electron microscope (SEM) findings of both upper respiratory tract tissue samples collected from plastinates and fresh material. When the data from scanning electron microscopy were analysed, cilia, cobblestone patterns, goblet cells and gland ducts on the epithelial surface were identified in areas on the upper respiratory tract. The SEM images of the plastinated tissues showed that the surface structures were degenerated due to the deformation of the surface epithelium. The plastination technique damaged the structures on the surface epithelium. Since the plastination technique and scanning electron microscopy have been studied together for the first time, we believe this would contribute to the scientific literature.

Microscopic Focus on the Thyroid Follicles of the One-Humped Camel (Camelus dromedarius).

The microstructure of the thyroid gland of the one-humped camel (Camelus dromedarius) was described using morphometric, histological, immunohistochemical staining, and ultrastructural standard techniques. The follicular secretory units of the thyroid glands displayed comparable and variable sizes where the large follicles were situated at the peripheral margins; however, the small ones were located in the central region. Semisquamous epithelium (low cuboidal) lined the large thyroid follicles, while high cuboidal (columnar) epithelium lined the small ones. Our electron microscopic findings revealed that the low-sized cuboidal follicular cells lack organelles and are hypoactive. The high cuboidal follicular cells are active cells and rich in cellular organelles such as cisternae of rough endoplasmic reticulum, mitochondria, colloid droplets, scrolled Golgi apparatus, and secretory vesicles. A few degenerate follicular cells appeared on rare occasions. The parafollicular cells appeared with a more prominent and conspicuous nucleus than the follicular cells. The follicular cells were classified as active, inactive, or degenerated using transmission electron microscopy. The follicular and parafollicular cells showed calcitonin-positive immunoreactivity. Overall, the presented results showed particular convergences of the morphostructural aspects of the thyroid gland of C. dromedarius to that of other mammals with some distinctive features to cope with their physiology and harsh niche.

Histopathologic and transmission electron microscopic findings in monkeypox cutaneous lesions.

a few pathologic and ultrastructural findings of monkeypox skin lesions are available in the literature. To integrate such evidence, we aimed to describe the pathologic features of monkeypox skin lesions and to show monkeypox virions by transmission electron microscopy (TEM). we studied the cutaneous biopsies of three patients affected by monkeypox during the 2022 monkeypox outbreak. Skin biopsies have been collected only from body sites with a recent laboratory-confirmed mpox virus infection, defined by a polymerase chain reaction (PCR) positive result in specimens taken through skin swabs. in all the samples the epidermis showed keratinocytes ballooning degeneration; perivascular/periadnexal infiltrates composed of neutrophils and lymphocytes were observed in the deep dermis. Immunohistochemistry showed that the infiltrate was mostly composed of CD3+ T-cells. TEM revealed monkeypox virus-like particles in various stages of morphogenesis in the dermis and epidermis; virions were interspersed among keratinocytes and within their cytoplasm. At the intracellular level, virions showed a biconcaveshaped central core, surrounded by lateral bodies and an external membrane; they also appeared as rectangular, brick-shaped, or oval particles with eccentric nucleoids. The histologic features of our skin samples confirmed the few other studies on this topic, except for the eosinophilic inclusions of the cytoplasm of keratinocytes (Guarnieri's bodies). the role of molecular biology is crucial for monkeypox diagnosis but when it is not disposable and/or in doubtful cases, skin biopsy and TEM may be helpful to establish the diagnosis.

The Effect of Calcium Hydroxide Pastes on Isolated Vital Nerve Fibers

IntroductionCalcium hydroxide pastes (CHPs), commonly used for disinfecting root canals during endodontic treatment, are generally considered safe. However, accidental extrusions result in minimal injuries and little to no discomfort, except when extruded pastes come into contact with nerve bundles, such as the inferior alveolar nerve. Currently, there is a lack of information about the possible role of specific paste vehicles on the extent of nerve injury. The purpose of this study was to compare the role that paste vehicles, such as water or methylcellulose, may play when nerve fibers are exposed to CHP. MethodsIsolated sciatic nerves of Sprague-Dawley rats were exposed to either water-based or methylcellulose-based CHP for varying durations of time (30, 60, or 90 minutes). Histopathological changes, including axonal edema, myelin alterations, and loss of cellular outlines, were assessed, and the degrees of changes were compared using chi-square intraclass correlation coefficient tests. ResultsBoth groups exposed to the pastes demonstrated varying degrees of histopathologic changes, including axonal edema, myelin changes, and loss of cellular outlines, at different exposure times. The water-based calcium hydroxide paste induced these changes more rapidly than the methylcellulose-based paste. Similar patterns were observed in the scanning electron microscopic findings. Exposure time emerged as an important difference in the effects of the 2 pastes. In each of these tests, all observations of water-based paste exposure were rated as moderate to severe, whereas the observed cellular changes (axonal, myelin, and intact cellular outline) were rated as mild to moderate after exposure to methylcellulose-based paste for the same exposure durations. The chi-square tests indicated a statistically significant association between the material and each of the outcomes (axonal changes: χ²15 = 81.0, P < .001; myelin changes: χ²15 = 81.0, P < .001; intact cellular outline, χ²15 = 81.0, P < .001). The intraclass correlation coefficient value was 0.93. ConclusionsThe study demonstrates that axonal and myelin damage increase with longer exposure times, with water-based CHP causing more damage than methylcellulose-based CHP at each time point.

Insulin granule morphology and crinosome formation in mice lacking the pancreatic β cell-specific phogrin (PTPRN2) gene.

We recently reported that phogrin, also known as IA-2β or PTPRN2, forms a complex with the insulin receptor in pancreatic β cells upon glucose stimulation and stabilizes insulin receptor substrate 2. In β cells of systemic phogrin gene knockout (IA-2β-/-) mice, impaired glucose-induced insulin secretion, decreased insulin granule density, and an increase in the number and size of lysosomes have been reported. Since phogrin is expressed not only in β cells but also in various neuroendocrine cells, the precise impact of phogrin expressed in β cells on these cells remains unclear. In this study, we performed a comprehensive analysis of morphological changes in RIP-Cre+/-Phogrinflox/flox (βKO) mice with β cell-specific phogrin gene knockout. Compared to control RIP-Cre+/- Phogrin+/+ (Ctrl) mice, aged βKO mice exhibited a decreased density of insulin granules, which can be categorized into three subtypes. While no differences were observed in the density and size of lysosomes and crinosomes, organelles involved in insulin granule reduction, significant alterations in the regions of lysosomes responding positively to carbohydrate labeling were evident in young βKO mice. These alterations differed from those in Ctrl mice and continued to change with age. These electron microscopic findings suggest that phogrin expression in pancreatic β cells plays a role in insulin granule homeostasis and crinophagy during aging, potentially through insulin autocrine signaling and other mechanisms.

Studying the Structure Properties of Copper Nanoparticles Prepared by Chemical Reduction Method

The chemical reduction method (CRM) can produce high purity nanoparticles, it is a down-top chemical method based on the principle of salt reduction, this method is used in this work to synthesis copper nanoparticals (CuPNs) by use different concentrations of copper nitrate. The properties of the prepared nanoparticles were studied by fourier transform infrared (FTIR) and ultraviolet-visible (UV-Vis) spectroscopy were used to measure the absorbance spectra of the produced particles. According to the scanning electron microscope (SEM), the particles’ diameters range from (61.64 to 49.25) nm. According to X-ray diffraction, the particles exhibit a face monoclinic crystal structure (FCC). According to the transmission electron microscope findings, the particles are asymmetrically spherical in shape.

Synthesis of ZnO Crystal with Micro‐Nano Structures by a Continuous Co‐Precipitation Method: Morphological Evolution and Mechanism Analysis

AbstractZnO crystals are employed in numerous industries, but precisely controlling their morphologies is still challenging. In this work, a continuous flow process for synthesizing ZnO crystals is developed based on the co‐precipitation method. ZnO crystals at different morphologies, including needle‐like, rod‐like, flower‐like, spindle‐like, and nanospheres, are successfully prepared at low temperature. The influence of reaction temperature, pH value, ZnCl2 feeding concentration, and reaction time on the morphology, size, and purity of ZnO crystals is systematically investigated. The results of X‐ray diffraction show that the high‐purity ZnO can only be produced when the reaction temperature is above 75 °C. The band gap energies of ZnO evaluated by UV–vis diffuse reflectance spectroscopy increase with the synthesis temperature. According to scanning electron microscope (SEM) findings, the size of ZnO crystals is regulated by controlling the reaction time. Interestingly, the morphology of ZnO crystals is changed from 1D to 3D structure by controlling the feeding concentration of ZnCl2. Moreover, a concentration difference mechanism of ZnO crystal morphology evolution is proposed by monitoring the real‐time concentration of free Zn2+ ions.

Dermoscopic and Electron Microscopic Findings of Black dot Tinea Capitis by Trichophyton tonsurans

Dermoscopic and Electron Microscopic Findings of Black dot Tinea Capitis by Trichophyton tonsurans

PS-B06-2: IN VIVO MYOCARDIAL EFFECTS OF INTRAPERITONEAL ARGLABIN VIA NLPR3 INFLAMMASOME AND MAPK/IKKB;/NFKB PATHWAYS

Introduction: Myocardial injury is a consequence of multiple underlying events that lead immune response by activated immune cells. Oxidative stress triggers an inflammatory cascade and apoptosis, this exacerbates cardiac injuries and dysfunction. Arglabin is a sesquiterpene lactone, an anticancer compound shown to have potential anti-diabetic effects in in vivo model. Methods: Male Wistar Rats in the 6 out of 9 groups were pre-treated with different doses (2.5 μg/kg, 5 μg/kg and 10 μg/kg of Arglabin) for 21 days. other 3 groups were normal control, vehicle and isoproterenol control. On 22nd day, isoproterenol was injected to induced myocardial injury (85 mg/kg/day). Normal control and isoproterenol control groups. Histopathological examination, electron microscopy, biomarkers (cTnI and LDH), cardiac parameters, oxidative stress (MDA, GSH, SOD, and CAT), inflammatory mediators (IL-6, IL-1β;, and TNF α), and apoptotic markers (BAX, Bcl2, and caspase-3) were evaluated. Results: Histopathological examination revealed increased neutrophilic infiltration in arglabin pretreated groups that correlated with electron microscopic findings. increased activity of IL-6, IL-1β;, TNFα, Caspase-1, caspase-3 and BAX was found in different pre-treated group. increased JNK expression was observed in arglabin pre-treated groups (2.5,5,10). altered expression of NFκB, P38, pP38 and IKKβ; were observed. Conclusion: Arglabin administration altered myocardial structure and modulated myocardial function via NLRP3 and MAPK pathway. Consideration regarding cardiovascular adverse effects must be undertaken when exploring other potential role of arglabin. Also, continuous cardiovascular monitoring is suggested for those on anti-cancer treatment with arglabin.

Electron Microscopic Study in the Rat Model of Electrically Injured Myelopathy: Preliminary Report.

The patient with electrically injured myelopathy showed mild motor weakness without somatosensory pathway abnormalities. Few reports have been reported on the pathophysiological mechanisms of electrically injured myelopathy, and there is controversy about the exact pathological causes. This study aimed to investigate the ultrastructural changes in the electron microscopic findings of electrical spinal cord injury. Nine rats were used in this study. We performed 7 electrical shocks (frequency, 120 Hz; pulse width, 0.9 ms; duration, 3 seconds; current, 99 mA) using an electroconvulsive therapy (ECT) apparatus (57800 ECT unit; UGO BASILE). We used one ear and one contralateral hind limb as entry and exit sites, respectively. We only enrolled rats with hind limb weakness and performed electron microscopy evaluations of the spinal cord on the first day and 4 weeks after injury. On the first day after injury, an electron microscopic examination showed a directly damaged area that appeared to be torn as physical damage, damaged myelin sheath, vacuolated axons in the myelin sheath, swollen Golgi apparatus, and injured mitochondria. Looking at changes in motor and sensory nerves, the sensory neurons showed recovered mitochondria and Golgi apparatus 4 weeks after injury; however, motor neurons still showed injured mitochondria, swollen Golgi apparatus, and endoplasmic reticulum. This study showed that recovery from ultrastructural injury was more rapid in sensory neurons than in motor neurons.

Macroanatomy of Cecum in German Mast Geese (Anser Anser) and Investigation with Scanning Electron Microscope (Sem)

Our study aimed to compare the cecum of German mast geese with other poultry species through macroanatomy and scanning electron microscope (SEM). Adult German geese were used as research material, which were obtained from its breeders and died of various causes. In the study, after determining the macroanatomical features of the German mast goose cecum, it was examined with a scanning electron microscope (JEOL JSM-5600LV) and the general structures were photographed. The craniodorsal of the rectum of German mast geese was shaped with two ceca, giving right and left arms to the cranial symmetrical to each other near the end of the ileum. The macroanatomy of the apex, corpus, and basis cecum parts was examined in the cecum divided into two. In the study, it was determined that villus extensions were sparse in the right and left apex ceci parts in the cecum SEM images of the German mast geese, while the number of the corpus and basis ceci parts was higher. As a result, the macroanatomy and scanning electron microscopic findings of German mast geese cecum were examined in detail and its differences from other poultry species were revealed.

The inhibitory effects of tyrosol on clinical Candida glabrata planktonic and biofilm cells

Biofilm formation is an important problem in the healthcare industry and veterinary medicine and is very common in natural, industrial or hospital environments. Microorganisms can become very resistant to antimicrobials and environmental factors by biofilm forming on biotic or abiotic surfaces. There is a need to develop new, effective and specific antimicrobials that can reduce pathogenicity in biofilm formation that threatens public health due to their role in medical device-related or infectious diseases. Candida species are opportunistic pathogenic yeasts and can cause superficial or disseminated infections. Especially C. glabrata is one of the most common microorganisms causing fungal infections in immunocompromised patients and drug resistance is observed when associated with biofilm. Tyrosol (2-[4-hydroxyphenyl] ethanol) can act as both a quorum sensing molecule and an exogenous agent on Candida species. In this study, the antifungal activity of tyrosol against a clinical C. glabrata isolate was investigated on both planktonic and biofilm forms. Broth microdilution test results demonstrated the inhibitory effect of tyrosol on C. glabrata. Transmission electron microscopic findings showed that tyrosol affected the planktonic C. glabrata cells in a multi targeted manner, and in the groups treated with tyrosol, significant damage was observed in the cell wall, cell membrane, cytoplasm, nucleus and mitochondria. Also, scanning electron microscopic images confirmed biofilm reduction in pre-/post-biofilm applications as a result of tyrosol treatment. In conclusion, tyrosol may be a potential alternative candidate for reducing the C. glabrata biofilm.

Clinical, histopathologic, immunohistochemical, and electron microscopic findings in cutaneous monkeypox: A multicenter retrospective case series in Spain

The worldwide outbreak of monkeypox has evidenced the usefulness of the dermatologic manifestations for its diagnosis. To describe the histopathologic and immunohistochemical findings of monkeypox cutaneous lesions. This is a retrospective histopathologic and immunohistochemical study of 20 patients with positive Monkeypox virus DNA polymerase chain reaction and immunohistochemical positivity for Vaccinia virus in cutaneous lesions. Four cases were also examined by electron microscopy. The most characteristic histopathologic findings consisted of full-thickness epidermal necrosis with hyperplasia and keratinocytic ballooning at the edges. In some cases, the outer root sheath of the hair follicle and the sebaceous gland epithelium were affected. Intraepithelial cytoplasmic inclusion bodies and scattered multinucleated keratinocytes were occasionally found. Immunohistochemically, strong positivity with anti-Vaccinia virus antibody was seen in the cytoplasm of ballooned keratinocytes. Electron microscopy study demonstrated numerous viral particles of monkeypox in affected keratinocytes. Small sample size. Electron microscopic study was only performed in 4 cases. Epidermal necrosis and keratinocytic ballooning are the most constant histopathologic findings. Immunohistochemical positivity for Vaccinia virus was mostly detected in the cytoplasm of the ballooned keratinocytes. These findings support the usefulness of histopathologic and immunohistochemical studies of cutaneous lesions for diagnosis of monkeypox.

Effects of Curcumin on Changes in Spermatogenetic Cells of Rats Treated with Cisplatin

Cisplatin is an effective antineoplastic drug which is used to treat many types of cancer. The most common known side effect of this drug is infertility. Curcumin, also called turmeric, has antioxidant and antitumor activities. This study was designed to investigate the effects of curcumin on the changes in spermatogenetic cells caused by cisplatin, which is used as a chemotherapeutic drug especially in testicular cancers. For this purpose, randomly selected 36 Spraque Dawley rats were seperated to 4 groups that of control, cisplatin, curcumin and curcumin+cisplatin and each group was seperated to 3 subgroups, each of them have 3 rats, so total 12 subgroups were obtained. The testicular tissues obtained from rats were evaluated histochemically, immunohistochemically and ultrastructually. In the results of this study, it is observed that the rate of apoptosis increased significantly in the experimental groups given curcumin together with cisplatin injection, and the transition-1 protein which is involved in DNA packaging in the elongated spermatids belonging to stages of XII, XIII an XIV, immunopositivity is increased statistically in the cisplatin-administered experimental groups compared to control groups. Both light and electron microscopic findings showed that intense degeneration, vacuolization, germ cell loss and spermatogenetic arrest occurred especially in the group given curcumin together with cisplatin. The results of our study showed that cisplatin can cause problems in infertility through DNA packaging and dragging cells to apoptosis and necrosis. Physicians who use cisplatin for cancer treatment are advised not to use curcumin together with cisplatin because it exacerbates the side effects of the cancer drug.