Electron Flow Research Articles

Proteomic analysis of endothelial cells and extracellular vesicles in response to indoxyl sulfate: Mechanisms of endothelial dysfunction in chronic kidney disease

AimsCardiovascular pathology is the main cause of death in chronic kidney disease (CKD) patients. CKD is associated with the accumulation of uremic toxins in the bloodstream, and indoxyl sulfate (IS) is one of the most abundant uremic toxins found in the blood of CKD patients. We conducted an in vitro study to assess the mechanisms underlying the IS-induced endothelial dysfunction that could lead to cardiovascular diseases. We also studied their extracellular vesicles (EVs) owing to their capacity to act as messengers that transmit signals through their cargo. Main methodsEVs were characterized by nanoparticle tracking analysis, transmission electron microscopy, flow cytometry, and tetraspanin expression. Cell lysates and isolated EVs were analyzed using liquid chromatography coupled with mass spectrometry, followed by Gene Set Enrichment Analysis to identify the altered pathways. Key findingsProteomic analysis of endothelial cells revealed that IS causes an increase in proteins related to adipogenesis, inflammation, and xenobiotic metabolism and a decrease in proliferation. Extracellular matrix elements, as well as proteins associated with myogenesis, response to UV irradiation, and inflammation, were found to be downregulated in IS-treated EVs. Fatty acid metabolism was also found to be increased along with adipogenesis and inflammation observed in cells. SignificanceThe treatment of endothelial cells with IS increased the expression of proteins related to adipogenesis, inflammation, and xenobiotic metabolism and was less associated with proliferation. Furthermore, EVs from cells treated with IS may mediate endothelial dysfunction, since they present fewer extracellular matrix elements, myogenesis, inflammatory factors, and proteins downregulated in response to UV radiation.

Using vertical farming systems for propagating everbearing strawberry: Physiological and technological feasibility assessment

BACKGROUND: One of the biggest challenges facing the soft fruit sector is to propagate disease free strawberry plants in a timely manner, financially viable approach and in an eco-friendly fashion. OBJECTIVE: This study sets out to investigate the efficacy of growing everbearing Fragaria×ananassa ‘Malling Ace’ runners at different propagation dates and under Different LED lighting regimes using vertical farming units and glasshouse (as control). METHODS: To evaluate the effects of the treatment factors, some of the main horticultural features including rooting success rate, rooting speed, root and shoot biomass production, chlorophyll content and bud emergence were measured and statistically analysed. In addition, this study aimed to investigate the energy efficiency of a self-sufficient Direct Current (DC) based vertical farming unit, versus a vertical farming unit using the Alternate Current (AC) electricity provided by national grid electricity. RESULTS: light, tipping date, and the interaction between light and tipping date, significantly influenced shoot biomass, total crown diameter, and chlorophyll content in the leaves of ‘Malling Ace’. In terms of energy demand, the DC unit had a higher level of energy efficiency over AC unit with 23% less energy demand. CONCLUSION: In general, this trial demonstrated vertical farming as a potentially sustainable method and efficient tool in propagating strawberry plants.

Experimental demonstration of an electroacoustic transistor

We experimentally demonstrate a topologically protected electroacoustic transistor. We construct a reconfigurable phononic analog of the quantum valley-Hall insulator composed of electrically shunted piezoelectric disks bonded to a patterned plate forming a monolithic structure. The device can be dynamically reconfigured to host one or more topological interface states via breaking inversion symmetry through selective powering of shunt circuits. Above a threshold, the amplitude of wave energy at a chosen location in one topological interface creates a second interface by dynamically switching power between two groups of shunts using relays. This enables the flow of wave energy between two locations in the reconfigured interface analogous to the voltage-controlled electron flow in a field effect transistor. The amplitude of wave energy in the second interface is used for bit abstraction to implement acoustic logic. We illustrate the various states of the transistor and experimentally demonstrate wave-based switching. The proposed electroacoustic transistor is envisioned to find applications in wave-based devices and edge computing in extreme environments and inspire novel technologies leveraging acoustic logic.

Self-Evolving Hierarchical Hydrogel Fibers with Circadian Rhythms and Memory Functions.

The fusion of hierarchical tissues at interfaces, incorporating ultrafast selective transport channels, enables efficient matter exchange and energy transfer across multiscale structures in living organisms. However, achieving these characteristics simultaneously in an artificial multimaterial system is challenging. Here, this work presents a multimaterial hydrogel fiber with a hierarchical structure of interface fusion, which forms spontaneously through in situ hierarchy evolution induced by ionic cross-linking and molecular shear flow. Water transport occurs in the angstrom-scale confined slits created by aligned cellulose nanocrystals (CNCs) by direct Coulomb knock-on, resembling Newton's cradle motion. The fusion of interfaces enables high-efficiency water transport across multiscale layers, combined with Newton's cradle-like collective water motion, creating a highly sensitive negative feedback loop within the fiber. These fibers exhibit integrated behaviors of time-space perception, short-term memory and adaptive changes in shape. Additionally, they demonstrate rhythm characteristics, changing periodically in a 24-h day-night cycle. Composed of natural building blocks, these hierarchical hydrogel fibers exhibit a memristor effect similar to that of an elementary neuron, making them promising for applications in seamless on-skin and implantable bioelectronics.

Integration of Renewable Energy Technologies for Sustainable Development in South Africa: A Focus on Grid-Connected PV Systems

Energy is essential for crucial development in Africa. The current electricity shortages and load shedding in South Africa show that the country faces significant challenges in reaching positive economic growth. For industries to operate sustainably, an innovative mechanism must be tailored to solve the negative impacts of industrial energy use, particularly climate change. This study aims to show how renewable energy technologies can provide new economic opportunities, contribute to higher standards of living, and reduce the impacts of society on ecosystems, among other things. This paper presents a feasibility analysis and optimization of new energy technologies by designing and simulating a grid-connected PV system for sustainable development. PV Syst software (PV Syst 6.8.8) was used to simulate and optimize the PV system. The software was employed to design and model the PV systems, calculating energy production, economic performance, and environmental impact. Using simulation data, the researchers compared PV system performance across three scenarios and identified the optimal system. Scenario A was chosen as the best system, with an energy production of 1720 MWh/year. Overall, the findings of this study suggest that grid-connected PV systems are a feasible and sustainable option for meeting South Africa’s energy needs. By implementing the results and recommendations, the government, investors, and community can work together to develop and deploy a successful PV system that will benefit all.

Dense Polarized Positrons from Beam-Solid Interaction.

Relativistic positron sources with high spin polarization have important applications in nuclear and particle physics and many frontier fields. However, it is challenging to produce dense polarized positrons. Here we present a simple and effective method to achieve such a positron source by directly impinging a relativistic high-density electron beam on the surface of a solid target. During the interaction, a strong return current of plasma electrons is induced and subsequently asymmetric quasistatic magnetic fields as high as megatesla are generated along the target surface. This gives rise to strong radiative spin flips and multiphoton processes, thus leading to efficient generation of copious polarized positrons. With three-dimensional particle-in-cell simulations, we demonstrate the production of a dense highly polarized multi-GeV positron beam with an average spin polarization above 40% and nC-scale charge per shot. This offers a novel route for the studies of laserless strong-field quantum electrodynamics physics and for the development of high-energy polarized positron sources.

First results of multi‐fluid modeling of detached hydrogen plasmas in a linear plasma device using fluid code LINDA‐NU

AbstractIn order to simulate hydrogen (H) plasma in the linear plasma device NAGDIS‐II, we have modified the fluid code LINDA‐NU to allow the simultaneous calculation of multiple ion species consisting of hydrogen atomic ions () and molecular ions (). In this simulation, H and neutrals are assumed to be uniformly distributed in space in order to obtain initial qualitative results. The fraction of ions increases as the molecular density increases, and the recombination process between and electrons is observed to reduce the particle flux to the target plate. With an increase in H density, the electron density increases due to the decrease in ion flow velocity due to the change exchange process, and the electron temperature decreases to less than 1 eV, leading to the detached plasma formation attributed to the electron‐ion recombination process.

Effect of Co9Se8 components on electrochemical and physical-chemical properties of porous CoCHH-Co9Se8 heterogeneous-tubes

Two factors affect the electrochemical performance of electrode materials of supercapacitors, the chemical composition and the structural design. The controllable construction of vacancy defects and porous structures on the surface of electrode materials is a new challenge. Three-dimensional interwoven porous CoCHH-Co9Se8 heterogeneous-tubes structures grow on the carbon cloth as a flexible electrode with abundant active sites and large surface area (154.2 m2 g−1). According to the experimental characterization results and kinetic analysis, the abundant vacancy defects and the porous structure provide plenty effective active sites and channels for ion flow, reaction and ion transport. With increase of Co9Se8 composition, the specific surface area of electrode material increases, and the multiple chemical compositions affect the performance of supercapacitors. The interwoven porous CoCHH-Co9Se8 heterogeneous-tubes electrode has excellent specific capacitance of 2561 F g−1. The energy density of the assembled flexible capacitor is 62.1 Wh kg−1 and the power density is 375.1 W kg−1. This work of controllable construction of pore structure and vacancy defects provides a new method for electrode preparation.

Thermoeconomic analysis of sCO2 power cycles

AbstractThe second‐law analysis evaluates the irreversibilities of a process. Systematic study of the relationship between thermodynamic efficiency and process modifications enhances process synthesis. The Allam cycle is an oxy‐fuel combustion cycle with nearly complete carbon capture that offers greater efficiency than current electricity generating systems. This study applies lost work analysis to the original Allam cycle and three modifications to obtain the distribution of irreversibilities and the effects of different configurations among potential process improvements for more sustainable power generation. The major inefficiencies are from the combustors and heat exchangers. We also examine the economic profitability of the alternatives. The largest equipment costs are for the turbines, compressors, and recuperators. We find that improving efficiency leads to less economic return; a configuration with partial compression has the highest efficiency, while the original Allam cycle has the highest profitability. We discuss how to resolve this apparent conflict between sustainability and profitability.

Pengaruh Media PhET Simulation Terhadap Hasil Belajar Fisika Siswa Kelas 12 MIA/SMAIT Yapira Raudhatut Tauhid

This research aims to determine the effect of PhET simulation on student learning outcomes in direct current electricity material at SMAIT Yapira Raudhatut Tauhid. The research method used in this research is an experimental method with a quantitative approach. The population in this study was class 12 students at SMAIT Yapira Raudhatut Tauhid with the sample size being class 12-3 with a total of 30 students, as the control class and class 12-2 with a total of 30 students as the experimental class. The results of the research show that there is a significant influence of the PhET Simulation media on the physics learning outcomes of class 12 students at MIA/SMAIT Yapira Raudhatut Tauhid. This can be proven by obtaining a value of t.count > t.table = 4.827 > 2.048.

Biogenic Palladium Improved Perchlorate Reduction during Nitrate Co-Reduction by Diverting Electron Flow in a Hydrogenotrophic Biofilm.

Microbial reduction of perchlorate (ClO4-) is emerging as a cost-effective strategy for groundwater remediation. However, the effectiveness of perchlorate reduction can be suppressed by the common co-contamination of nitrate (NO3-). We propose a means to overcome the limitation of ClO4- reduction: depositing palladium nanoparticles (Pd0NPs) within the matrix of a hydrogenotrophic biofilm. Two H2-based membrane biofilm reactors (MBfRs) were operated in parallel in long-term continuous and batch modes: one system had only a biofilm (bio-MBfR), while the other incorporated biogenic Pd0NPs in the biofilm matrix (bioPd-MBfR). For long-term co-reduction, bioPd-MBfR had a distinct advantage of oxyanion reduction fluxes, and it particularly alleviated the competitive advantage of NO3- reduction over ClO4- reduction. Batch tests also demonstrated that bioPd-MBfR gave more rapid reduction rates for ClO4- and ClO3- compared to those of bio-MBfR. Both biofilm communities were dominated by bacteria known to be perchlorate and nitrate reducers. Functional-gene abundances reflecting the intracellular electron flow from H2 to NADH to the reductases were supplanted by extracellular electron flow with the addition of Pd0NPs.

Electron Dynamics in Alkane C-H Activation Mediated by Transition Metal Complexes.

Alkanes, ideal raw materials for industrial chemical production, typically exhibit limited reactivity due to their robust and weakly polarized C-H bonds. The challenge lies in selectively activating these C-H bonds under mild conditions. To address this challenge, various C-H activation mechanisms have been developed. Yet, classifying these mechanisms depends on the overall stoichiometry, which can be ambiguous and sometimes problematic. In this study, we utilized density functional theory calculations combined with intrinsic bond orbital (IBO) analysis to examine electron flow in the four primary alkane C-H activation mechanisms: oxidative addition, σ-bond metathesis, 1,2-addition, and electrophilic activation. Methane was selected as the representative alkane molecule to undergo C-H heterolytic cleavage in these reactions. Across all mechanisms studied, we find that the CH3 moiety in methane consistently uses an electron pair from the cleaved C-H bond to form a σ-bond with the metal. Yet, the electron pair that accepts the proton differs with each mechanism: in oxidative addition, it is derived from the d-orbitals; in σ-bond metathesis, it resulted from the metal-ligand σ-bonds; in 1,2-addition, it arose from the π-orbital of the metal-ligand multiple bonds; and in electrophilic activation, it came from the lone pairs on ligands. This detailed analysis not only provides a clear visual understanding of these reactions but also showcases the ability of the IBO method to differentiate between mechanisms. The electron flow discerned from IBO analysis is further corroborated by results from absolutely localized molecular orbital energy decomposition analysis, which also helps to quantify the two predominant interactions in each process. Our findings offer profound insights into the electron dynamics at play in alkane C-H activation, enhancing our understanding of these critical reactions.

Effects of temporal IFNγ exposure on macrophage phenotype and secretory profile: exploring GMP-Compliant production of a novel subtype of regulatory macrophages (MregIFNγ0) for potential cell therapeutic applications

BackgroundMacrophages are involved in tissue homeostasis, angiogenesis and immunomodulation. Proangiogenic and anti-inflammatory macrophages (regulatory macrophages, Mreg) can be differentiated in-vitro from CD14+ monocytes by using a defined cell culture medium and a stimulus of IFNγ.Aim of the studyTo scrutinize the potential impact of temporal IFNγ exposure on macrophage differentiation as such exposure may lead to the emergence of a distinct and novel macrophage subtype.MethodsDifferentiation of human CD14+ monocytes to Mreg was performed using a GMP compliant protocol and administration of IFNγ on day 6. Monocytes from the same donor were in parallel differentiated to MregIFNγ0 using the identical protocol but with administration of IFNγ on day 0. Cell characterization was performed using brightfield microscopy, automated and metabolic cell analysis, transmission electron microscopy, flow cytometry, qPCR and secretome profiling.ResultsMreg and MregIFNγ0 showed no differences in cell size and volume. However, phenotypically MregIFNγ0 exhibited fewer intracellular vesicles/vacuoles but larger pseudopodia-like extensions. MregIFNγ0 revealed reduced expression of IDO and PD-L1 (P < 0.01 for both). They were positive for CD80, CD14, CD16 and CD38 (P < 0.0001vs. Mreg for all), while the majority of MregIFNγ0 did not express CD206, CD56, and CD103 on their cell surface (P < 0.01 vs. Mreg for all). In terms of their secretomes, MregIFNγ0 differed significantly from Mreg. MregIFNγ0 media exhibited reduced levels of ENA-78, Osteopontin and Serpin E1, while the amounts of MIG (CXCL9) and IP10 were increased.ConclusionExposing CD14+ monocytes to an alternatively timed IFNγ stimulation results in a novel macrophage subtype which possess additional M1-like features (MregIFNγ0). MregIFNγ0 may therefore have the potential to serve as cellular therapeutics for clinical applications beyond those covered by M2-like Mreg, including immunomodulation and tumor treatment.

Passivation Layers in Mg-Metal Batteries: Robust Interphases for Li-Metal Batteries.

In advanced batteries, interphases serve as the key component in stabilizing the electrolyte with reactive electrode materials far beyond thermodynamic equilibria. While an active interphase facilitates the transport of working ions, an inactive interphase obstructs ion flow, constituting the primary barrier to the realization of battery chemistries. Here, a successful transformation of a traditionally inactive passivating layer on Mg-metal anode, characteristic of Mg-metal batteries with typical carbonate electrolytes, into an active and robust interphase in the Li-metal scenario is presented. By further strategically designing magnesiated Li+ electrolytes, the in situ development of this resilient interphase on Li-metal anodes, imparting enduring stability to Li-metal batteries with nickel-rich cathodes is induced. It is identified that the strong affinity between Mg2+ and anions in magnesiated Li+ electrolytes assembles ionic clusters with a bias for reducibility, thereby catalyzing the creation of anion-derived interphases rich in inorganic constituents. The prevalence of ionic clusters induced by magnesiation of electrolytes has brought properties only available in high-concentration electrolytes, suggesting a fresh paradigm of tailing electrolytes for highly reversible LMBs.

Rod-like Mn0.2Cd0.8S wrapped in foliated CeO2 promote efficient photocatalytic hydrogen evolution

The Mn0.2Cd0.8S/CeO2 heterojunction is synthesized by means of hydrothermal coupling. Short rod-like Mn0.2Cd0.8S is wrapped in leaf like CeO2 to construct a tight heterointerface, which stimulated the photocatalytic activity to the great extent. Due to the remarkable catalytic and electrochemical properties of Mn0.2Cd0.8S, the CeO2 surface active site is driven. CeO2 has excellent specific surface area, suitable pore volume aperture and Mn0.2Cd0.8S match to optimize the internal interface charge transfer path. At the same time, the heterointerface can coordinate the interface electron flow and promote the H2 reduction ability. The results show that the optimal hydrogen production of Mn0.2Cd0.8S/CeO2 with S-type heterostructure is 291.8 μmol, and the internal lattice and surface morphology of Mn0.2Cd0.8S/CeO2 are not easily damaged due to good catalytic stability. The photocatalytic mechanism of Mn0.2Cd0.8S/CeO2 is studied by finding the short distance interfacial charge transfer path and enriching the photogenerated electrons required for hydrogen evolution.

Enhanced Ionic Power Generation via Light-Driven Active Ion Transport Across 2D Semiconductor Heterostructures.

2D semiconductor heterostructures exhibit broad application prospects. However, regular nanochannels of heterostructures rarely caught the researcher's attention. Herein, a metal-organic framework (i.e., Cu3(HHTP)2) and transition metal dichalcogenides (i.e., MoS2)-based multilayer van der Waals heterostructure (i.e., Cu3(HHTP)2/MoS2) realized band alignment-dominated light-driven ion transport and further light-enhanced ionic energy generation. High-density channels of the heterostructure provide high-speed pathways for ion transmembrane transport. Upon light illumination, a net ionic flow occurs at a symmetric concentration, suggesting a directional cationic transport from Cu3(HHTP)2 to MoS2. This is because Cu3(HHTP)2/MoS2 heterostructures containing type-II band alignment can generate photovoltaic motive force through light-induced efficient charge separation to drive ion transport. After introducing into the ionic power generation system, the maximum power density under illumination can achieve notable improvement under different concentration differences. In addition to the photovoltaic motive force, type-II band alignment and material defect capture-induced surface charge increase also raise ion selectivity and flux, greatly facilitating ionic energy generation. This work demonstrates that 2D semiconductor heterostructures with rational band alignment can not only be a potential platform for optimizing light-enhanced ionic energy harvesting but also provide a new thought for biomimetic iontronic devices.

Dynamics of Ion and Electron Flows in the Penning Discharge Chamber

Dynamics of Ion and Electron Flows in the Penning Discharge Chamber

Flux Ratios for Effects of Permanent Charges on Ionic Flows with Three Ion Species: Case Study (II)

Flux Ratios for Effects of Permanent Charges on Ionic Flows with Three Ion Species: Case Study (II)

Organic Electrosynthesis: A Promising Green Tool in Solving Key Steps for the Total Synthesis of Complex Natural Products

Abstract: Electro-organic synthesis, an atom-efficient, sustainable, mild process, permits an ecofriendly and elegant green path to synthesize structurally complex, still valuable molecules, avoiding the use of conventional harsh oxidizing and reducing agents and long-route reaction protocols. Being one of the oldest forms of reaction setups in a laboratory, it deals with fundamental redox chemistry through the direct application of electrical potential. Here flow of electrons acts as an oxidizing agent at the anode at the same time reducing agent at the cathode, depending upon the requirement of the reaction. Simultaneously, it minimizes the generation of reagent waste during the reaction. However, electrifying organic synthesis plays more than preventing the waste footprint. This technology provides an alternative roadmap through nonclassical bond disconnections to access desired target molecules by cutting down a number of steps with the formation of apparently looking difficult bonds with excellent regio-, chemo-and stereoselectivity. Hence, it emerges as an alternative and attractive technique for the contemporary synthetic communities. Consequently, in recent years, multiple milestones have been achieved in the electro-organic synthesis of fascinating natural products through oxidative C-C bond formation, C-H/N-H functionalization, very rare oxidative N-N dimerization, RCDA dimerization, etc. Thus, synthesis of extremely complex natural products through finding new electro-synthetic route as a key methodology have become one of the alluring synthetic targets to synthetic chemists because of their versatile utilities in medicine, agriculture, food, and cosmetic industry. This review presents advances in electrochemistry in the total synthesis of 20 complex natural products reported since 2013. Enabling synthetic steps are analyzed alongside innate advantages as well as future prospects are speculated.

A Model of High-Energy Electron Flows in GLONASS Orbits

A Model of High-Energy Electron Flows in GLONASS Orbits