A carbon nanofiber functionalized with ruthenium(II)-tetrasulfonato phenyl porphine (CNF/Ru-TSPP) is shown to be viable sensor for amperometric determination of the antipsychotic drug chlorpromazine (CPZ). The hollow platelet structured Ru-TSPP combines with the hollow cylindrical tube-like structure of the CNF via Ï stacking interaction. The morphological and electro conductive properties of the electrode were characterized by spectrophotometric techniques. The CNF/Ru-TSPP modified electrode displays a large surface-to-volume ratio, good electron transport and good electrocatalytic activity. The amperometric sensor, typically operated at a potential 0.63V (vs. Ag/AgCl) exhibits a linear response in the 0.6nM to 1.1mM CPZ concentration range, has a 0.2nM detection limit, and a remarkably good electrochemical sensitivity (2.405ÎŒAÎŒM-1cm-2). The sensor is selective, repeatable and reproducible. It was successfully applied to the determination of CPZ in spiked serum samples. Graphical abstract Schematic presentation of carbon nanofiber/ tetrasulfonatophenyl Ruthenium(II)porphine (CNF/Ru-TSPP) nanocomposite synthesis and application for the electrochemical determination of chlorpromazine(CPZ).