This paper presents and experimentally validates a highly sensitive fiber-optic Fabry-Perot interferometer sound sensor based on a fiber-optic spherical structure. The Fabry-Perot interferometer comprises an aluminum foil diaphragm and a fused fiber-optic microsphere end face, both sealed in a structure made of a glass tube. An optical fiber microsphere structure is used for the first time in acoustic sensing. Based on the strong focusing capability of the microsphere, the high coupling efficiency to the reflected beam, and the extensive range of the focused convergent optical field formed, the focused convergent optical fields facilitate the sensing of an extensive range of motion or deformation of the cavity compared to the divergent optical field of a flat fiber end face, i.e., the acoustic signals at more minor sound pressures can also be well sensed, thus compared to a flat fiber end face fiber The acoustic sensor can monitor acoustic signals over a more extensive frequency range and a larger range of acoustic pressure variations, and the output voltage signal is of a larger amplitude at the same acoustic signal, which allows the monitoring of acoustic signals at acoustic frequencies within the hearing range of the human ear. Experimental results show that the sensor shows a flat frequency response in the range of 20 Hz–20 kHz, covering all frequency components of the human ear hearing range, and the signal-to-noise ratio of the proposed acoustic sensor in the range of 20 Hz–20 kHz is greater than that of the flat fiber end-surface based acoustic sensor and the reference electroacoustic sensor, especially The sensor has the advantages of good high sensitivity, excellent linearity, wide planar response range, and simple manufacturing process. It can potentially be used as a highly sensitive, high acoustic quality fiber optic microphone in practical applications.