Electrowetting-on-dielectric Research Articles

Emerging Trends in Integrated Digital Microfluidic Platforms for Next-Generation Immunoassays

Technologies based on digital microfluidics (DMF) have made significant advancements in the automated manipulation of microscale liquids and complex multistep processes. Due to their numerous benefits, such as automation, speed, cost-effectiveness, and minimal sample volume requirements, these systems are particularly well suited for immunoassays. In this review, an overview is provided of diverse DMF manipulation platforms and their applications in immunological analysis. Initially, droplet-driven DMF platforms based on electrowetting on dielectric (EWOD), magnetic manipulation, surface acoustic wave (SAW), and other related technologies are briefly introduced. The preparation of DMF is then described, including material selection, fabrication techniques and droplet generation. Subsequently, a comprehensive account of advancements in the integration of DMF with various immunoassay techniques is offered, encompassing colorimetric, direct chemiluminescence, enzymatic chemiluminescence, electrosensory, and other immunoassays. Ultimately, the potential challenges and future perspectives in this burgeoning field are delved into.

Optimizing Bipolar Reset Waveform to Improve Grayscale Stability in Active Matrix Electrowetting Displays.

The electrowetting display (EWD) device is a new type of electrowetting-on-dielectric (EWOD) equipment that can achieve a paper-like display effect under the control of an electric field. In this microfluidic system, the stability of grayscale can be affected by various factors, such as the physicochemical properties of the materials, the device structure, and electric field distribution. To improve the grayscale stability of active matrix electrowetting displays (AM-EWDs), the impact of different polarities of driving voltage on oil backflow was investigated in this study. Based on the driving principles of AM-EWD, an optimized inter-frame bipolar reset driving waveform was designed to overcome oil backflow. The proposed driving waveform maintained the stability of the oil state by periodically and rapidly releasing trapped charges in the dielectric layer through a reverse driving voltage. Additionally, the effect of feed-through voltage on pixel driving voltage was eliminated by compensating for the driving voltage on a common electrode. Finally, the performance of the designed driving waveform was evaluated with a 6-inch AM-EWD driving platform. Compared to the conventional unipolar reset driving waveform, the backflow speed decreased by 2.70 a.u./s. The standard deviation of the display luminance was also reduced by 11.24 a.u. Experimental results indicated that both the oil backflow speed and the fluctuation range of luminance were effectively suppressed by the proposed driving waveform.

Dielectric constant enhancement of BaTiO3/SU-8 for low-voltage droplet actuation

Digital microfluidics (DMF) technology, which called lab-on-a-chip will bring technological innovations to the field of biochemistry. Electro-wetting-on-dielectric (EWOD) is one of the forms of DMF which actuate independent droplets through electric field enabling more accurate and efficient actuation. Nowadays, the high driving voltage required for droplet actuation remains a major obstacle to the practical application of this technology. Dielectric layer is the main factor affecting the driving voltage. In this study, we fabricate a dielectric layer film that can reduce the driving voltage by doping BaTiO3 nanoparticles with high dielectric constant in SU-8 at room temperature. The volume ratio of dopant particles and spin coating speed are studied to determine the optimal processing conditions for low-voltage droplet actuation. As the volume ratio of the dopant particles increased, the dielectric layer dielectric constant firstly increases and then decreases. When the volume ratio of BaTiO3:SU-8 is 1:10, the maximum dielectric constant is achieved. The dielectric wetting properties follow the same trend as the change in dielectric layer dielectric constant. Thus, the parameters of a volume ratio of 1:10 and spin coating speed of 7500 rad/min are finally selected for the preparation of the dielectric layer. These measures resulted in lower threshold voltages for droplet actuation, which contributes to advancing the application and widespread adoption of DMF technology.

Non-mechanical beam steering for high-speed optical wireless communications via electrowetting on dielectric.

Electrowetting on dielectric (EWOD) is used for non-mechanical optical beam steering (OBS) in optical communication systems. High-capacitance ion gel is used to construct an efficient electrowetting interface that facilitates dynamic OBS. This integration facilitates precise control of beam steering and data transmission efficiency in optical wireless communication systems. An EWOD-based liquid prism (LP) manipulates beam direction via electrowetting. The theoretical framework is supported by the Young and Young-Lippmann equations for precise optical path control. We present a theoretical and experimental demonstration of a two-dimensional beam steering system using an EWOD-based LP, with beam steering up to 14.82° and 14.39° along the X and Y axes, respectively. The system achieves data rates of 1.9 Gbps in free-space optics (FSO) and 1.7 Gbps in underwater wireless optical communication (UWOC) systems, with a measured bit error rate that adheres to the standard threshold of the forward error correction limit. Our results suggest that the EWOD-based LP system offers a compact, efficient, and versatile design for optical devices in both FSO and UWOC systems.

Instability and breakup of rayleigh-plateau jets with capillary wave induced by electrowetting-on-dielectric

This paper discusses the lateral disturbances of vertical liquid flow enhancement using electrowetting-on-dielectric (EWOD) technique, which effectively reducing the breakup length of the liquid flow and minimizing the formation of satellite droplets. The EWOD technology is characterized by its minimal space occupation and low energy consumption. The successful implementation of the EWOD phenomenon relies on the formation of a three-phase contact line (TPCL) between the electrodes and the liquid, and the novel EWOD tube structure design provides an extended TPCL, enhancing the amplitude of capillary waves. Through experimental and theoretical analysis, this paper identifies key parameters affecting the liquid breakup process, such as the gap depth, angle of the EWOD tube, EWOD voltage amplitude, and frequency of the AC electrical signal. The research results indicate that the proposed EWOD tube responds rapidly and can consistently and stably manage liquid breakup and reduce the number of satellite droplets.

A Novel EWOD Platform for Freely Transporting Droplets in Double and Single-Plate Structures.

This study developed a novel dielectric wetting microfluidic operation platform combining parallel-plate and coplanar-plate regions with a curved surface structure as the connection structure. With the new electrowetting on dielectric (EWOD) platform, "droplet pull-out" has been successfully achieved and viewed as an essential new operation for microfluidics with the dielectric wetting technique. The EWOD system is divided into a PDMS substrate top plate and an indium tin oxide (ITO) glass substrate as a bottom layer on this chip. In the parallel-plate region, the droplets can be generated and transported through the square parallel electrodes; in the single-plate area, the droplets can be pulled out from the parallel structure, transported and mixed through the common grounded coplanar electrodes. In dielectric wetting performance testing, coplanar electrodes can apply a maximum driving force of 31.22 µN to DI water and 13.38 µN to propylene carbonate (PC). This driving force is sufficient to detach the sample from the top cover and pull the sub-droplet from the parallel plate structure for DI water, PC and polyethylene glycol diacrylate (PEGDA) buffer. The novel EWOD system also possesses the advantage of precise volume control for liquid samples; the volume error of the generated droplet can be controlled within 0.1% to 2%.

Orbital Electrowetting-on-Dielectric for Droplet Manipulation on Superhydrophobic Surfaces.

Electrowetting-on-dielectric (EWOD), recognized as the most successful electrical droplet actuation method, is essential in diverse applications, ranging from thermal management to microfluidics and water harvesting. Despite significant advances, it remains challenging to achieve repeatability, high speed, and simple circuitry in EWOD-based droplet manipulation on superhydrophobic surfaces. Moreover, its efficient operation typically requires electrode arrays and sophisticated circuit control. Here, a newly observed droplet manipulation phenomenon on superhydrophobic surfaces with orbital EWOD (OEW) is reported. Due to the asymmetric electrowetting force generated on the orbit, flexible and versatile droplet manipulation is facilitated with OEW. It is demonstrated that OEW droplet manipulation on superhydrophobic surfaces exhibits higher speed (up to 5 times faster), enhanced functionality (antigravity), and manipulation of diverse liquids (acid, base, salt, organic, e.g., methyl blue, artificial blood) without contamination, and good durability after 1000 tests. It is envisioned that this robust droplet manipulation strategy using OEW will provide a valuable platform for various processes involving droplets, spanning from microfluidic devices to controllable chemical reactions. The previously unreported droplet manipulation phenomenon and control strategy shown here can potentially upgrade EWOD-based microfluidics, antifogging, anti-icing, dust removal, and beyond.

A low-cost electrowetting on dielectric semi-continuous pump for application to microfluidic reconfigurable devices

An electrowetting on dielectric (EWOD) pump is a microfluidic pump that uses electrowetting to manipulate liquid droplets in a channel, offering an alternative approach to traditional mechanical pumps. EWOD-based pumps have significant potential for various microfluidic applications. For instance, by integrating microfluidic pumps with a radio frequency (RF) device, it is possible to create a microfluidic reconfigurable RF device. However, the current microfluidic-based devices have been primarily designed based on mechanical micropumps which require expensive clean-room fabrication methods. Here, we present an analytical model for an EWOD semi-continuous pump that can offer a promising alternative actuation for microfluidic actuation. However, a literature survey of recent advances in EWOD pumping has highlighted a gap in the modeling of the relationship between the fluid mechanics and the actuation dynamics. This paper presents an analytical model of an EWOD pump that determines the flow rate and pressure generated by taking into account the competing electrowetting force, friction force, and fluid inertia force in a one-degree-of-freedom channel. The analytical model is valuable for designing and optimizing EWOD-based pumps, as it provides insights into the dominant physical processes and could enable better control. The model is validated with an EWOD experiment and the data demonstrates less than a 6 % error between the measured and predicted maximum droplet velocity and a maximum 7.4 % error in the EWOD pump static pressure.

Electrowetting-on-dielectric behavior of aqueous droplets and gold nanofluid on an electrospun poly(vinyl chloride) microfiber layer

Morphology and wettability of dielectric layers are crucial properties that affect the electrowetting-on-dielectric (EWOD) performance of a working liquid. In this work, the use of a poly(vinyl chloride) (PVC) microfiber-modified PVC dielectric layer as an electrowetting-on-dielectric (EWOD) substrate is explored. Imaging with scanning electron and atomic force microscopy revealed intertwined microfibers on the original PVC film after PVC deposition via electrospinning. Hydrophobicity of the PVC layer was enhanced by the presence of microfibers, with the contact angle (CA) for a water droplet increasing from 84.9° to 123.9°. EWOD behavior of various liquids on the microfiber-modified PVC layer was investigated within a DC voltage range of 0 to 200 V DC. Gold nanofluid exhibited the largest change in CA of 57°, while smaller changes were observed for KOH (19.6°), KCl (21.2°), and water (21°). A mechanism explaining the observed reduction in contact angle for a gold nanofluid droplet is presented. Our results suggest the promising potential of PVC film-PVC microfiber as a hydrophobic dielectric material for EWOD applications involving diverse liquids, including gold nanofluids.

A Review of Nanostructures in Electrowetting-on-dielectric Systems: From Nanostructured Dielectric Layers to Nanofluids

Abstract: The extensive interest in electrowetting-on-dielectric (EWOD) as a key in advancing the efficiency and controllability of fluid-based microelectromechanical and actuator systems has resulted in a deluge of technological research, especially in the area of microfluidics, liquid lenses, and fluid-based lab-on-chips. More recently, the integration of nanostructures into EWOD-driven devices has shown promising improvement in these devices’ performance, design, and miniaturization. Due to the exceptional properties, availability, versatility, and tunability of nanostructures, they are being utilized as components of EWOD systems for various applications. Utilization ranges from fabricating nanodimensional dielectric layers to incorporating nanoparticles in fluid droplets. With the current trend in improving the performance and functionality of EWOD-driven devices at low voltage operations, it is timely to revisit the fundamental principle of EWOD phenomena and how it is extended experimentally using nanostructures. In this paper, we present the different nanostructures investigated as dielectric materials in various EWOD experiments focusing on metal oxide and silicon nitride layers. Notes on the structure of these dielectric layers are also presented. Furthermore, various EWOD experiments employing nanofluid droplets are also described. This paper provides a clear picture of nanostructures’ diverse impact on the advancement of EWOD technology. The insights presented in this paper may also serve as a guidepost for future exploration and development of the role of nanostructures in EWOD-driven devices.

Electric-field induced droplet stable vertical vibration: Experiments and numerical simulations

In this study, stable vertical vibration of a single droplet was stimulated through Electrowetting on Dielectric (EWOD) on a Polydimethylsiloxane-coated Indium Tin Oxide glass (PDMS-ITO) surface in an oil atmosphere. The mechanism of the vibration is clarified as a self-adjusted energy earning and losing equilibrium, and a small friction at the contact line is crucial for this stable vibration. We investigated the impact of needle tip electrode height, different voltage forms (AC and DC voltages), and voltage magnitude on the droplet vibration state. As decreasing the needle tip electrode height, there is a transition of the droplet from a vibrating state to a static equilibrium state. During the vibration, the vibration period remains constant regardless of tip height, but the contact angle changes synchronously with the voltage. The electric signal in the circuit is also measured to provide solid evidence for this synchronicity. Finally, the saturation voltage of the contact angle and amplitude is about 120 V. The intrinsic connection between contact angle saturation and amplitude saturation is clarified by studying the surface energy of the droplet. A theoretical model is constructed to numerically simulate the shape and amplitude of the droplet’s vibrations.

Switchable Optical Properties of Dyes and Nanoparticles in Electrowetting Devices.

The optical properties of light-absorbing materials in optical shutter devices are critical to the use of such platforms for optical applications. We demonstrate switchable optical properties of dyes and nanoparticles in liquid-based electrowetting-on-dielectric (EWOD) devices. Our work uses narrow-band-absorbing dyes and nanoparticles, which are appealing for spectral-filtering applications targeting specific wavelengths while maintaining device transparency at other wavelengths. Low-voltage actuation of boron dipyromethene (BODIPY) dyes and nanoparticles (Ag and CdSe) was demonstrated without degradation of the light-absorbing materials. Three BODIPY dyes were used, namely Abs 503 nm, 535 nm and 560 nm for dye 1 (BODIPY-core), 2 (I2BODIPY) and 3 (BODIPY-TMS), respectively. Reversible and low-voltage (≤20 V) switching of dye optical properties was observed as a function of device pixel dimensions (300 × 900, 200 × 600 and 150 × 450 µm). Low-voltage and reversible switching was also demonstrated for plasmonic and semiconductor nanoparticles, such as CdSe nanotetrapods (abs 508 nm), CdSe nanoplatelets (Abs 461 and 432 nm) and Ag nanoparticles (Abs 430 nm). Nanoparticle-based devices showed minimal hysteresis as well as faster relaxation times. The study presented can thus be extended to a variety of nanomaterials and dyes having the desired optical properties.

Electrowetting hysteresis on a deformable dielectric film.

Electrowetting on dielectric (EWOD) involves actuating droplets through electrical stimulus while the droplets lie on a dielectric layer that covers the electrode. In order to activate the droplet, a higher threshold voltage is required due to electrowetting hysteresis. The effect of a deformable dielectric layer and its thickness on electrowetting hysteresis has not been studied. We demonstrate in this work that electrowetting hysteresis increases with decreasing elasticity of the dielectric layer. This is attributed to the formation of wetting ridges along the three-phase contact line and relies on electro-elastic capillary interactions. Furthermore, we demonstrate that the thickness of the dielectric film can affect electrowetting hysteresis on soft materials. In addition, we present a unique mechanism of electrowetting hysteresis reduction on soft solids by the addition of a minute amount of anionic surfactant (sodium dodecyl sulfate, SDS) to the liquid. We also demonstrate that the voltage cycle affects the electrowetting hysteresis of sessile drops on a soft dielectric layer. Dielectric layer elasticity can be used in various engineering applications, such as optofluidic displays, liquid lenses, lab-on-a-chip devices, and energy harvesting systems.

Democratizing digital microfluidics by a cloud-based design and manufacturing platform.

Akin to the impact that digital microelectronics had on electronic devices for information technology, digital microfluidics (DMF) was anticipated to transform fluidic devices for lab-on-a-chip (LoC) applications. However, despite a wealth of research and publications, electrowetting-on-dielectric (EWOD) DMF has not achieved the anticipated wide adoption, and commercialization has been painfully slow. By identifying the technological and resource hurdles in developing DMF chip and control systems as the culprit, we envision democratizing DMF by building a standardized design and manufacturing platform. To achieve this vision, we introduce a proof-of-concept cloud platform that empowers any user to design, obtain, and operate DMF chips (https://edroplets.org). For chip design, we establish a web-based EWOD chip design platform with layout rules and automated wire routing. For chip manufacturing, we build a web-based EWOD chip manufacturing platform and fabricate four types of EWOD chips (i.e., glass, paper, PCB, and TFT) to demonstrate the foundry service workflow. For chip control, we introduce a compact EWOD control system along with web-based operating software. Although industrial fabrication services are beyond the scope of this work, we hope this perspective will inspire academic and commercial stakeholders to join the initiative toward a DMF ecosystem for the masses.

Chip-integrated non-mechanical microfluidic pump driven by electrowetting on dielectrics.

A microfluidic pump is presented that generates its pumping action via the EWOD (electrowetting-on-dielectric) effect. The flow is generated by the periodic movement of liquid-vapor interfaces in a large number (≈106) of microcavities resulting in a volume change of approx. 0.5 pl per cavity per pump stroke. The total flow resulting from all microcavities adds up to a few hundred nanolitres per cycle. Passive, topologically optimized, non-mechanical Tesla valves are used to rectify the flow. As a result, the micropump operates without any moving components. The dimensioning, fabrication, and characterization process of the micropump are described. Device fabrication is done using conventional manufacturing processes from microsystems technology, enabling cost-effective mass production on wafer-level without additional assembly steps like piezo chip-level bonding, etc. This allows for direct integration into wafer-based microfluidic or lab-on-a-chip applications. Furthermore, first measurement results obtained with prototypes of the micropump are presented. The voltage- and frequency-dependent pump performance is determined. The measurements show that a continuous flow rate larger than 0.2 ml min-1 can be achieved at a maximum pump pressure larger than 12 mbar.

Discretised microfluidics for noninvasive health monitoring using sweat sensing.

Using sweat instead of blood for monitoring chemical biomarker concentrations of hospitalised patients offers several advantages for both the patients and healthcare workers. Unlike blood, sweat can be noninvasively and continuously sampled without direct involvement of a professional, and sweat contains a rich composition of biomarkers. However, patients in resting state have extremely low sweat rates and they produce correspondingly small sweat volumes, which makes sweat sensing of hospitalised patients highly challenging. We propose a unique solution that enables the use of sweat as a viable biofluid for noninvasive health monitoring, by actively transporting the sweat in a discretised manner. Our device uses electrowetting-on-dielectrics (EWOD) to create and move sweat droplets with a volume of around 1 nanolitre from a sweat gland to sensors integrated in the device. We present the first wearable device with integrated EWOD, and we show that it can collect and transport sweat on-body, while measuring sweat rate, under conditions typical for individuals at rest.

Absolute quantification of nucleic acid on digital microfluidics platform based on superhydrophobic–superhydrophilic micropatterning

Digital microfluidics (DMF) shows great potential in the manipulation of individual droplets. However, the limited number of electrode control wirings for droplet control intrinsically restrains the massive generation of pL/nL individual droplets, fundamentally hindering the realization of high throughput applications on DMF such as digital nucleic acid amplification and analysis. This work demonstrates on-chip high throughput digital loop-mediated isothermal amplification (dLAMP) on a DMF platform based on a functional substrate with super-wettability contrast. Exploiting the passive dispensing technique as empowered by electrowetting on dielectric (EWOD) effect, a total of 1818 individual droplets with high uniformity are generated on the super-hydrophilic microarray within 1 min. Separation within the oil phase without tubes and valves can avoid evaporation, ensuring a precise total reaction volume. Absolute quantification of λDNA with a dynamic range of 1–1000 copies/μL is achieved by counting the amount of positive microdroplets after on-chip incubation, demonstrating the strong potential for rapid, low-cost, reliable, and quantitative nucleic acid analysis with high accuracy.

Flow Analysis and Structural Optimization of Double-Chamber Parallel Flexible Valve Micropumps

In the current study, a two-dimensional numerical study is carried out to investigate the performance of a novel Double-Chamber Parallel Flexible Valve micropump, which utilized the electrowetting-on-dielectrics (EWOD) effect to drive the microfluid flow. By observing the flow fields, the internal circulations are seen on both the left and right sides of the pump. The generation of the backflow is discussed as well by tracking the movement of the vortices. Only slight flow fluctuations are seen in the micropump. Based on the simulation results, the structural parameters including the width of the inlet and the outlet, the width of the pumping channel and the diverging angle in the micropump are analyzed, and the influence of these parameters on the pumping volume and the maximum pressure are discussed. Eventually, a group of optimal parameter combinations is given according to the simulation results to extend the operating potential of the micropump.

Triboelectric nanogenerator-based self-powered two-dimensional microfluidic system for biochemical reaction

Microfluidic systems for biochemical reactions require efficient microfluidic actuation. However, current microfluidic devices are facing challenges in terms of self-powering and precise transport of microfluidics. This study demonstrates a novel approach to fabricating a self-powered and precise microfluidic manipulation system based on triboelectric nanogenerator (TENG) and electrowetting-on-dielectric (EWOD) technologies. The TENG provides high pulsed voltage and electrical wetting capabilities, enabling the manipulation system to drive microfluidic transport through electric field forces. The self-powered microfluidic device is systematically investigated by the effect of various parameters, including TENG power, TENG structures, EWOD/microfluidic device structures and surface hydrophobic properties. By optimizing the parameters, optimal performances are achieved in microfluidic motion, particularly in terms of precise two-dimensional movement. The movable volume range spans from 20 nl to 1.4 ml, representing one of the most notable achievements of TENG in the field so far. Additionally, the self-powered microfluidic system successfully synthesized multiple morphological ZnO nanoparticles, a rarely reported accomplishment. Finally, the novel self-powered system demonstrates the capability to detect chloride in disinfected water and COVID-19 antigen detection. This work proposes a novel TENG-EWOD microfluidic control technology that shows promise in various applications, including chemical processes, biological health testing and microdevice fabrication.

Inkjet-printed disposable electrowetting-on-dielectric chip for nervous necrosis virus detection using immuno Raman sensing beads and magnetic nanoparticle trapping

BackgroundDroplet-based electrowetting-on-dielectric (EWOD) methods avoid the problem of requiring pumps or valves in the lab-on-a-chip technology. However, current EWOD chips are expensive, hindering their applications to disposable use. In this work, a cost-effective inkjet-printed EWOD paper chip was created to lower costs. Its application to detect nervous necrosis virus (NNV) in aquaculture has been demonstrated. MethodsUsing an office inkjet printer, an EWOD paper chip patterned with conducting pads on glossy label paper was integrated into a biosensing platform, facilitating NNV detection via EWOD sample preparation prior to Raman analysis. The method involved a sandwich assay, initiating binding between antibody-functionalized magnetic nanoparticles and NNV through droplet vibration, magnetic trapping the NNV-bound nanoparticles and supernatant removal, and further binding of Raman sensing beads with NNV to form sandwich-like nanocomplexes for Raman micro-spectrophotometer detection. FindingsAn inkjet-printed paper chip, costing less than US $0.2, achieves a remarkable detection limit of 15,600 TCID50/mL for NNV, two orders of magnitude superior to existing immunoassays with similar cost. While a fiber optic nanoplasmonic immunosensor offers similar sensitivity, it comes at a threefold higher per-test cost. These findings support the cost-effective use of the paper-based EWOD method for routine pond water monitoring.